BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	669,635	A→G	V54A (GTG→GCG)	ybeN ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	669,635	0	A	G	80.9%	466.5 / 118.5	247	V54A (GTG→GCG)	ybeN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (23/24); new base G (92/107); total (116/131)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

Only 100 of 252 total aligned reads displayed.

TAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTC  >  NC_000913/669545‑669726

 aaGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTgcgcgc                                                                                          <  2:2086992/93‑1 (MQ=255)

   gagGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTTTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTg                                                                                        >  1:4100194/1‑93 (MQ=255)

   gagGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTTCGCCATATCCCGTTCAAACATGTGTTTACGCTGCGCGCTg                                                                                        >  1:364066/1‑93 (MQ=255)

   gagGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTg                                                                                        <  2:1076467/93‑1 (MQ=255)

   gagGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTg                                                                                        <  2:459259/93‑1 (MQ=255)

   gagGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTg                                                                                        <  1:1719682/93‑1 (MQ=255)

    agGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGt                                                                                       <  1:4929825/93‑1 (MQ=255)

     gggggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGtt                                                                                      >  2:2175301/1‑93 (MQ=255)

      ggggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTc                                                                                     >  2:1521126/1‑93 (MQ=255)

      ggggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTc                                                                                     <  2:1137429/93‑1 (MQ=255)

       gggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTTTTGGCAATCGCCCGGTCAAGCATGTGTTTTCGCTGCGCGCTGTTCg                                                                                    >  1:5148871/1‑93 (MQ=255)

       gggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCg                                                                                    >  2:2607899/1‑93 (MQ=255)

        ggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGc                                                                                   <  1:5269557/93‑1 (MQ=255)

        ggCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGc                                                                                   <  1:4503083/93‑1 (MQ=255)

         gCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGGTGTTCCCt                                                                                  >  2:2329853/1‑93 (MQ=255)

           aTTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTc                                                                                <  2:1901988/93‑1 (MQ=255)

            ttGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTcc                                                                               <  1:1578620/93‑1 (MQ=255)

               cgcTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTTTTGGCAATCGCCAGTTCAAACATGTGTTTACGCTGCGCGCTGTTTGCTTCCGGc                                                                            >  1:7168218/1‑93 (MQ=255)

               cgcTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCAAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGc                                                                            <  1:2253817/93‑1 (MQ=255)

                gcTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCt                                                                           <  2:4191881/93‑1 (MQ=255)

                 cTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTTTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCCGCTg                                                                          >  1:3512308/1‑93 (MQ=255)

                 cTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTg                                                                          >  2:3824460/1‑93 (MQ=255)

                 cTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTg                                                                          >  1:7129303/1‑93 (MQ=255)

                 cTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATACGCAGATTAAGCATGTGGTTACGCTTCGCGCCGTTCGCTTTCGGCTg                                                                          >  1:5318596/1‑93 (MQ=255)

                  tttAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTgg                                                                         >  2:5368300/1‑93 (MQ=255)

                   ttAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTggg                                                                        <  2:810565/93‑1 (MQ=255)

                   ttAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTggg                                                                        <  2:5967961/93‑1 (MQ=255)

                   ttAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTggg                                                                        >  2:634967/1‑93 (MQ=255)

                    tAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTgggg                                                                       <  2:1687195/93‑1 (MQ=255)

                     aGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGa                                                                      <  2:1285794/93‑1 (MQ=255)

                     aGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGa                                                                      <  2:5693152/93‑1 (MQ=255)

                       cTCGCGTTCATCAAGAGTAAATAATGGCTTGTTGGCAATCGCCAGTTTAAGCATGTGTTTTCGCTGCGCGCTGTTTGCTTCCGGCTGGGGGCg                                                                    >  1:5044635/1‑93 (MQ=255)

                       cTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACg                                                                    <  1:2674392/93‑1 (MQ=255)

                        tCGCGTTCATCAAGAGTAAATAATGGCTTTTCGGCAAACGCCCGTTCAAGCATGTGTTTTCGCTGCGCGCTGTTTGCTTCCCGCTGGGGGCgg                                                                   >  1:2796926/1‑92 (MQ=255)

                        tCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGa                                                                   <  1:5658405/93‑1 (MQ=255)

                        tCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGa                                                                   <  1:6383275/93‑1 (MQ=255)

                        tCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGa                                                                   <  2:5410616/93‑1 (MQ=255)

                         cgcgTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGAt                                                                  <  1:1615786/93‑1 (MQ=255)

                          gcgTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATg                                                                 >  2:4957313/1‑93 (MQ=255)

                          gcgTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATg                                                                 >  2:6097474/1‑93 (MQ=255)

                          gcgTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATg                                                                 <  2:2681818/93‑1 (MQ=255)

                           cgTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGc                                                                >  2:4057300/1‑93 (MQ=255)

                             ttCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCgg                                                              <  2:5571376/93‑1 (MQ=255)

                              tcatcaAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCgga                                                             <  1:212888/93‑1 (MQ=255)

                              tcatcaAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCgga                                                             <  1:1084689/93‑1 (MQ=255)

                               catcaAGAGTAAATAATGGCTTGTCGGGAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGGCGGTGCgggg                                                            >  1:141227/1‑91 (MQ=255)

                                 tcaAGAGTAAATAATGGCTTTTCGGCAATCGCCCGTTCAAACATGTGTTTACGCTGCGCGCTGTTTGCTTCCGGCTGGGGGCGGTGCggggga                                                          >  1:6671308/1‑93 (MQ=255)

                                 tcaAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCggagga                                                          <  1:1011059/93‑1 (MQ=255)

                                  caAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGaa                                                         <  2:3376462/93‑1 (MQ=255)

                                   aaGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGGAc                                                        >  2:6672463/1‑93 (MQ=255)

                                    agagTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCCGCTGGGGGCGATGCGGGGGGACa                                                       >  1:5787717/1‑93 (MQ=255)

                                      agTAAATAATGGCTTTTTGGCAATCGCCCGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCCGCTGGGGGCGATGCGGGGGGACatt                                                     >  1:6486099/1‑93 (MQ=255)

                                      agTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACatt                                                     <  2:3523081/93‑1 (MQ=255)

                                       gTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACatta                                                    <  2:5176948/93‑1 (MQ=255)

                                       gTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACatta                                                    >  2:1964897/1‑93 (MQ=255)

                                         aaaTAATGGCTTTTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGGCGATGGGGGGGGACattatt                                                  >  1:779756/1‑93 (MQ=255)

                                         aaaTAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACattatt                                                  <  2:3907441/93‑1 (MQ=255)

                                          aataatGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACAttatta                                                 <  2:4661127/93‑1 (MQ=255)

                                          aataatGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACAttatta                                                 <  1:1288139/93‑1 (MQ=255)

                                           ataatGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGGCGATGCGGGGGGACATTATTAg                                                >  1:2642083/1‑93 (MQ=255)

                                           ataatGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAg                                                <  2:269981/93‑1 (MQ=255)

                                             aatGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCCGCTGGGGGCGATGCGGAGGGACATTATTAggg                                              >  1:4456310/1‑93 (MQ=255)

                                              atGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGga                                             <  1:1881324/93‑1 (MQ=255)

                                               tGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGgat                                            <  1:5743850/93‑1 (MQ=255)

                                                 gCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGgatga                                          <  1:1684409/93‑1 (MQ=255)

                                                 gCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGgatga                                          >  2:6517567/1‑93 (MQ=255)

                                                      tCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATtgtg                                     >  1:96468/1‑93 (MQ=255)

                                                         gCAATCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGAcc                                  <  1:2338416/93‑1 (MQ=255)

                                                            aTCGCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCcgc                               <  1:4658131/93‑1 (MQ=255)

                                                               gCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTc                            >  1:855091/1‑93 (MQ=255)

                                                               gCCAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTc                            <  1:3365042/93‑1 (MQ=255)

                                                                ccAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCa                           >  2:2829034/1‑93 (MQ=255)

                                                                ccAGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCa                           <  1:4394130/93‑1 (MQ=255)

                                                                  aGTTCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGa                         >  1:5474724/1‑93 (MQ=255)

                                                                  aGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGa                         >  2:3195388/1‑93 (MQ=255)

                                                                  aGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGa                         >  2:7134579/1‑93 (MQ=255)

                                                                    ttCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGAcc                       <  1:6928757/93‑1 (MQ=255)

                                                                     tCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCa                      >  2:1594453/1‑93 (MQ=255)

                                                                     tCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCa                      >  2:3119335/1‑93 (MQ=255)

                                                                     tCAAGCATGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCa                      >  2:7024071/1‑93 (MQ=255)

                                                                       aaGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAAt                    <  1:7324542/93‑1 (MQ=255)

                                                                          cATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCaa                 >  1:1314732/1‑93 (MQ=255)

                                                                           aTGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCaaa                <  2:6233823/93‑1 (MQ=255)

                                                                           aTGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCaaa                >  2:6162342/1‑93 (MQ=255)

                                                                           aTGTGTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCaaa                <  2:6809835/93‑1 (MQ=255)

                                                                            tgtgTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGGCGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAAt               >  1:823721/1‑93 (MQ=255)

                                                                            tgtgTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAAt               >  1:4069635/1‑93 (MQ=255)

                                                                            tgtgTTTACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAAt               >  1:6126024/1‑93 (MQ=255)

                                                                            tgtgTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAAt               >  1:6748536/1‑93 (MQ=255)

                                                                                tttACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGc           >  2:6878309/1‑93 (MQ=255)

                                                                                 ttACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGcc          >  1:1331558/1‑93 (MQ=255)

                                                                                 ttACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGcc          >  2:5122758/1‑93 (MQ=255)

                                                                                  tACGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCa         <  1:7203309/93‑1 (MQ=255)

                                                                                    cGCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGc       <  1:6181109/93‑1 (MQ=255)

                                                                                     gCTGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCg      <  2:4863353/93‑1 (MQ=255)

                                                                                      cTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGt     >  1:2945384/1‑93 (MQ=255)

                                                                                       tGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGtt    >  1:3852126/1‑93 (MQ=255)

                                                                                       tGCGCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGtt    >  2:2869405/1‑93 (MQ=255)

                                                                                       tGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGtt    >  2:4135936/1‑93 (MQ=255)

                                                                                       tGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGtt    <  2:731795/93‑1 (MQ=255)

TAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAGAGTAAATAATGGCTTGTCGGCAATCGCCAGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTC  >  NC_000913/669545‑669726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`