BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,801	A→G	D200D (GAT→GAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,801	0	A	G	85.7%	667.9 / 104.2	287	D200D (GAT→GAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base A (22/19); new base G (136/109); total (158/129)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

Only 100 of 292 total aligned reads displayed.

CGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGC  >  NC_000913/697710‑697892

  tAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCg                                                                                          >  2:4418609/1‑93 (MQ=255)

     ggCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAg                                                                                       <  1:1692745/93‑1 (MQ=255)

        acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCg                                                                                    >  2:2000288/1‑93 (MQ=255)

        acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCg                                                                                    >  1:3800971/1‑93 (MQ=255)

         catcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGa                                                                                   >  1:3047901/1‑93 (MQ=255)

          atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGaa                                                                                  >  2:6475739/1‑93 (MQ=255)

             agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCa                                                                               >  2:2401561/1‑93 (MQ=255)

             agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCa                                                                               >  2:5254162/1‑93 (MQ=255)

             agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCa                                                                               <  2:6560077/93‑1 (MQ=255)

              gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAg                                                                              >  1:4356565/1‑93 (MQ=255)

              gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAg                                                                              <  1:3860259/93‑1 (MQ=255)

               acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTTACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGt                                                                             <  2:2605264/93‑1 (MQ=255)

               acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGTTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGt                                                                             >  1:236919/1‑93 (MQ=255)

               acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCCCGTTATCTTTCACCGCGACGTAGACGAACCAGt                                                                             <  1:1021456/93‑1 (MQ=255)

               acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGt                                                                             >  2:187234/1‑93 (MQ=255)

                catcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTc                                                                            <  2:1930955/93‑1 (MQ=255)

                catcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTc                                                                            <  1:5261721/93‑1 (MQ=255)

                 atcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTcg                                                                           >  2:6164065/1‑93 (MQ=255)

                    agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                        >  2:1742403/1‑93 (MQ=255)

                    agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                        >  1:7080726/1‑93 (MQ=255)

                    agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                        <  1:4759955/93‑1 (MQ=255)

                     gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                       >  2:7435830/1‑93 (MQ=255)

                      aTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatg                                                                      >  2:3502510/1‑93 (MQ=255)

                       tGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatga                                                                     <  1:2319542/93‑1 (MQ=255)

                          cTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAg                                                                  >  2:5902357/1‑93 (MQ=255)

                           ttttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGt                                                                 <  1:42855/93‑1 (MQ=255)

                                aCTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGaa                                                            <  2:7274670/93‑1 (MQ=255)

                                 cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                           >  2:5178780/1‑93 (MQ=255)

                                  tGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACg                                                          >  2:6418985/1‑93 (MQ=255)

                                   gAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACgg                                                         <  1:5969125/93‑1 (MQ=255)

                                    aaTCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGa                                                        <  1:2125645/93‑1 (MQ=255)

                                     aTCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt                                                       >  1:3630422/1‑93 (MQ=255)

                                      tCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCTTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAtt                                                      <  1:6225394/93‑1 (MQ=255)

                                      tCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAtt                                                      <  2:166015/93‑1 (MQ=255)

                                       cTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt                                                     >  2:4529057/1‑93 (MQ=255)

                                       cTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt                                                     <  2:1120561/93‑1 (MQ=255)

                                          ccAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGc                                                  <  2:3034085/93‑1 (MQ=255)

                                           cAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGcc                                                 >  2:3867448/1‑93 (MQ=255)

                                            aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                                >  1:4044370/1‑93 (MQ=255)

                                            aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                                >  1:6529181/1‑93 (MQ=255)

                                            aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                                >  1:5839287/1‑93 (MQ=255)

                                            aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                                >  2:2963047/1‑93 (MQ=255)

                                             gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                               >  2:504413/1‑93 (MQ=255)

                                             gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                               >  2:4236552/1‑93 (MQ=255)

                                               gCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                             >  2:6262613/1‑93 (MQ=255)

                                               gCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                             <  2:1468656/93‑1 (MQ=255)

                                                ccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCt                                            <  2:3659164/93‑1 (MQ=255)

                                                 cTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTg                                           <  2:825000/93‑1 (MQ=255)

                                                  tGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                          >  1:273501/1‑93 (MQ=255)

                                                  tGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                          >  2:7047676/1‑93 (MQ=255)

                                                  tGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                          <  2:5451691/93‑1 (MQ=255)

                                                   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGc                                         <  1:3972547/93‑1 (MQ=255)

                                                   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGc                                         <  1:769413/93‑1 (MQ=255)

                                                    aCGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCt                                        <  2:2729786/93‑1 (MQ=255)

                                                    aCGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCt                                        >  2:954325/1‑93 (MQ=255)

                                                       cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCca                                     <  1:1489460/93‑1 (MQ=255)

                                                       cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCca                                     >  1:1350864/1‑93 (MQ=255)

                                                         gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcaca                                   >  2:6708171/1‑93 (MQ=255)

                                                         gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcaca                                   >  1:725663/1‑93 (MQ=255)

                                                          cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaa                                  >  2:63155/1‑93 (MQ=255)

                                                           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                                 <  2:1365500/93‑1 (MQ=255)

                                                           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                                 >  2:3938240/1‑93 (MQ=255)

                                                           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                                 <  2:5029922/93‑1 (MQ=255)

                                                           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                                 >  2:6065195/1‑93 (MQ=255)

                                                            cGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAAt                                <  1:5730421/93‑1 (MQ=255)

                                                             gTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATa                               <  2:4785401/93‑1 (MQ=255)

                                                              tttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAg                              >  2:5480609/1‑93 (MQ=255)

                                                              tttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAg                              <  2:3250259/93‑1 (MQ=255)

                                                              tttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAg                              >  1:2487869/1‑93 (MQ=255)

                                                                tttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCt                            >  1:3411327/1‑93 (MQ=255)

                                                                   gTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTccc                         >  1:6691251/1‑93 (MQ=255)

                                                                    tCGGTCACGTTATCTTTGACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                        >  2:4900759/1‑93 (MQ=255)

                                                                    tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                        <  1:5497155/93‑1 (MQ=255)

                                                                    tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                        <  2:6888805/93‑1 (MQ=255)

                                                                    tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                        <  2:5781267/93‑1 (MQ=255)

                                                                    tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                        >  1:1239386/1‑93 (MQ=255)

                                                                     cGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccg                       >  2:2820499/1‑93 (MQ=255)

                                                                       gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgcc                     >  1:7413741/1‑93 (MQ=255)

                                                                       gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgcc                     >  2:4040723/1‑93 (MQ=255)

                                                                       gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgcc                     >  1:6051444/1‑93 (MQ=255)

                                                                         cACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCgg                   <  1:6859343/93‑1 (MQ=255)

                                                                          aCGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTTCACAAATAGCTCCCCGCCggg                  >  1:4150314/1‑93 (MQ=255)

                                                                          aCGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCggg                  >  2:1528385/1‑93 (MQ=255)

                                                                           cGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGa                 <  2:2251001/93‑1 (MQ=255)

                                                                            gTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGaa                >  1:5233726/1‑93 (MQ=255)

                                                                             ttttCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAc               <  1:3261828/90‑1 (MQ=255)

                                                                             ttATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAc               <  1:620600/93‑1 (MQ=255)

                                                                             ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAc               >  1:1075245/1‑93 (MQ=255)

                                                                              tATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAct              >  1:2276090/1‑93 (MQ=255)

                                                                                tCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActct            >  1:3529409/1‑93 (MQ=255)

                                                                                tCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActct            <  1:1584620/93‑1 (MQ=255)

                                                                                 cTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt           <  2:5646925/93‑1 (MQ=255)

                                                                                    tCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa        >  1:264030/1‑93 (MQ=255)

                                                                                     cACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt       <  1:4470558/93‑1 (MQ=255)

                                                                                     cACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt       <  1:5033481/93‑1 (MQ=255)

                                                                                      aCCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc      >  2:7415718/1‑93 (MQ=255)

                                                                                      aCCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc      <  1:7487733/93‑1 (MQ=255)

                                                                                        cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt    >  1:2187004/1‑93 (MQ=255)

                                                                                        cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt    >  1:6364831/1‑93 (MQ=255)

                                                                                        cGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt    <  2:7366292/93‑1 (MQ=255)

CGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGC  >  NC_000913/697710‑697892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`