BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	708,813	C→T	D419D (GAC→GAT)	ybfM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	708,813	0	C	T	86.9%	870.7 / 80.2	297	D419D (GAC→GAT)	ybfM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (17/22); new base T (144/114); total (161/136)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

Only 100 of 298 total aligned reads displayed.

ACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCAAC  >  NC_000913/708721‑708903

aCCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGAc                                                                                            >  2:5975797/1‑93 (MQ=255)

   tGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGt                                                                                         <  2:4973748/93‑1 (MQ=255)

   tGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGt                                                                                         >  1:942132/1‑93 (MQ=255)

   tGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGt                                                                                         >  1:3412370/1‑93 (MQ=255)

        gagCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCgcgc                                                                                    >  2:6736360/1‑93 (MQ=255)

        gagCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCgcgc                                                                                    >  1:6194787/1‑93 (MQ=255)

        gagCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCgcgc                                                                                    >  2:4065410/1‑93 (MQ=255)

         agCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGcc                                                                                   <  2:5728477/93‑1 (MQ=255)

         agCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGcc                                                                                   >  2:2714302/1‑93 (MQ=255)

          gCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCa                                                                                  >  1:5269719/1‑93 (MQ=255)

            aaTCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCaaa                                                                                <  1:6998245/93‑1 (MQ=255)

             aTCCGGATGCGTACTACGGCAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAg                                                                               >  1:7172281/1‑93 (MQ=255)

             aTCCGGATGCGTACTACGACAAAAACCGGGCTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAg                                                                               >  2:7358288/1‑93 (MQ=255)

              tCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAgg                                                                              >  1:2569687/1‑93 (MQ=255)

               ccGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGc                                                                             >  2:249697/1‑93 (MQ=255)

               ccGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGc                                                                             <  1:6420885/93‑1 (MQ=255)

                cGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCa                                                                            <  2:5459251/93‑1 (MQ=255)

                cGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCa                                                                            <  1:274282/93‑1 (MQ=255)

                   aTGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGa                                                                         <  2:2943915/93‑1 (MQ=255)

                    tGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGAt                                                                        >  1:159146/1‑93 (MQ=255)

                    tGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGAt                                                                        >  2:3028373/1‑93 (MQ=255)

                     gCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATg                                                                       <  1:5672/93‑1 (MQ=255)

                      cGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGTCAAAGGCACGATGt                                                                      <  2:6441337/93‑1 (MQ=255)

                        tactacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTc                                                                    >  2:3741143/1‑93 (MQ=255)

                        tactacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTc                                                                    <  2:6942423/93‑1 (MQ=255)

                        tactacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTc                                                                    >  1:3071169/1‑93 (MQ=255)

                        tactacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTc                                                                    >  1:4625770/1‑93 (MQ=255)

                         actacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCa                                                                   >  1:1794765/1‑93 (MQ=255)

                          ctacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCaa                                                                  >  2:1727859/1‑93 (MQ=255)

                           tacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCaaa                                                                 <  1:4016635/93‑1 (MQ=255)

                           tacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCaaa                                                                 <  1:6745542/93‑1 (MQ=255)

                           tacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCaaa                                                                 <  1:5604995/93‑1 (MQ=255)

                           tacGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCaaa                                                                 >  2:5871567/1‑93 (MQ=255)

                            acGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAAc                                                                <  1:4268710/93‑1 (MQ=255)

                             cGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACt                                                               <  1:7259668/93‑1 (MQ=255)

                              gACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTg                                                              >  1:4366983/1‑93 (MQ=255)

                              gACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTg                                                              >  2:5603999/1‑93 (MQ=255)

                               aCAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGc                                                             >  2:4903025/1‑93 (MQ=255)

                                cAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCa                                                            <  1:4168054/93‑1 (MQ=255)

                                cAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCa                                                            <  2:1036932/93‑1 (MQ=255)

                                  aaaaCCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCAtt                                                          <  2:6203218/93‑1 (MQ=255)

                                     aCCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCa                                                       >  1:480141/1‑93 (MQ=255)

                                     aCCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCa                                                       <  2:7035658/93‑1 (MQ=255)

                                     aCCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCa                                                       <  1:6885993/93‑1 (MQ=255)

                                          aCTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGaa                                                  <  1:3494062/93‑1 (MQ=255)

                                            tATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATa                                                <  1:1079507/93‑1 (MQ=255)

                                            tATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATa                                                >  2:3979476/1‑93 (MQ=255)

                                            tATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATa                                                <  1:5372800/93‑1 (MQ=255)

                                             aTTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATAc                                               >  2:4100223/1‑93 (MQ=255)

                                                 aaGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACa                                           <  1:5198741/93‑1 (MQ=255)

                                                 aaGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACa                                           <  2:6113322/93‑1 (MQ=255)

                                                 aaGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACa                                           >  1:3875818/1‑93 (MQ=255)

                                                  agagTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACaa                                          >  1:2920975/1‑93 (MQ=255)

                                                        tGCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTc                                    <  2:2595209/93‑1 (MQ=255)

                                                         gCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTcc                                   >  1:1535024/1‑93 (MQ=255)

                                                         gCCTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTcc                                   <  2:1291354/93‑1 (MQ=255)

                                                          ccTACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCg                                  >  1:1914237/1‑93 (MQ=255)

                                                            tACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGAc                                >  2:791513/1‑93 (MQ=255)

                                                            tACAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGAc                                <  1:1734894/93‑1 (MQ=255)

                                                            tACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGAc                                <  2:1383586/93‑1 (MQ=255)

                                                              cAGCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACAt                              >  1:5858045/1‑93 (MQ=255)

                                                                gCCTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATcc                            >  1:2481748/1‑93 (MQ=255)

                                                                 ccTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATccc                           <  2:430896/93‑1 (MQ=255)

                                                                 ccTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATccc                           >  2:38908/1‑93 (MQ=255)

                                                                 ccTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATccc                           <  1:2287282/93‑1 (MQ=255)

                                                                  cTGGATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCa                          >  1:419920/1‑93 (MQ=255)

                                                                  cTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCa                          >  1:765781/1‑93 (MQ=255)

                                                                    ggATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAg                        <  2:3703184/93‑1 (MQ=255)

                                                                     gATGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGc                       >  2:5840193/1‑93 (MQ=255)

                                                                       tGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTg                     <  2:3316987/93‑1 (MQ=255)

                                                                       tGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTg                     <  1:313419/93‑1 (MQ=255)

                                                                       tGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTg                     >  1:7292420/1‑93 (MQ=255)

                                                                       tGCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATACCAAGCTg                     >  1:4071433/1‑93 (MQ=255)

                                                                        gCGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTgg                    <  2:4570812/93‑1 (MQ=255)

                                                                         cGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTggg                   <  1:2842320/93‑1 (MQ=255)

                                                                         cGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTggg                   >  2:1480304/1‑93 (MQ=255)

                                                                         cGGTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTggg                   <  2:4425450/93‑1 (MQ=255)

                                                                          tgTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTgggg                  <  2:510755/92‑1 (MQ=255)

                                                                          ggTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTgggg                  >  2:5275141/1‑93 (MQ=255)

                                                                           gTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGc                 <  2:5162500/93‑1 (MQ=255)

                                                                           gTCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGc                 >  1:1394189/1‑93 (MQ=255)

                                                                           gTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGc                 <  1:2184610/93‑1 (MQ=255)

                                                                            tCTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCg                >  2:2184056/1‑93 (MQ=255)

                                                                            tCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCg                <  1:3961597/93‑1 (MQ=255)

                                                                             cTATACCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCgg               >  2:4357038/1‑93 (MQ=255)

                                                                              tataCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggt              >  2:1661980/1‑93 (MQ=255)

                                                                               ataCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggtg             >  1:5510649/1‑93 (MQ=255)

                                                                               ataCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggtg             <  1:3612637/93‑1 (MQ=255)

                                                                               ataCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggtg             >  1:7411485/1‑93 (MQ=255)

                                                                               ataCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggtg             >  1:5902902/1‑93 (MQ=255)

                                                                                taCCATTCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCggtgg            >  1:5125270/1‑93 (MQ=255)

                                                                                     ttCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACg       <  2:1085528/93‑1 (MQ=255)

                                                                                     ttCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACg       >  2:1983322/1‑93 (MQ=255)

                                                                                     ttCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACg       >  2:3473956/1‑93 (MQ=255)

                                                                                      tCAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACgg      <  2:7204127/93‑1 (MQ=255)

                                                                                       cAGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGc     <  2:5535892/93‑1 (MQ=255)

                                                                                        aGGATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCa    >  1:5808023/1‑93 (MQ=255)

                                                                                         ggATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCaa   >  1:1222373/1‑93 (MQ=255)

                                                                                         ggATGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCaa   >  1:6337448/1‑93 (MQ=255)

                                                                                          gACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCAAc  >  2:6581489/1‑93 (MQ=255)

ACCTGGCAGAGCAATCCGGATGCGTACTACGACAAAAACCGGACTATTGAAGAGTCTGCCTACAGCCTGGATGCGGTCTATACCATTCAGGACGGTCGCGCCAAAGGCACGATGTTCAAACTGCATTTCACCGAATACGACAACCACTCCGACATCCCAAGCTGGGGCGGTGGTTACGGCAAC  >  NC_000913/708721‑708903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`