BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,321	A→G	I103M (ATA→ATG)	ybfN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,321	0	A	G	85.7%	744.7 / 111.0	314	I103M (ATA→ATG)	ybfN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (27/18); new base G (126/143); total (153/161)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 319 total aligned reads displayed.

CGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGT  >  NC_000913/709229‑709412

cgcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATa                                                                                             <  1:2092276/93‑1 (MQ=255)

    tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCa                                                                                         >  2:3647540/1‑93 (MQ=255)

    tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCa                                                                                         >  1:5256594/1‑93 (MQ=255)

    tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCa                                                                                         <  2:5154782/93‑1 (MQ=255)

     aNTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCan                                                                                        <  2:4799637/93‑2 (MQ=255)

     aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAg                                                                                        >  1:5447799/1‑93 (MQ=255)

     aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAg                                                                                        >  1:3329339/1‑93 (MQ=255)

      cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGt                                                                                       >  2:7062601/1‑93 (MQ=255)

      cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGt                                                                                       >  1:7164029/1‑93 (MQ=255)

       tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGTCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGta                                                                                      >  1:306259/1‑93 (MQ=255)

         gATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGtaat                                                                                    <  1:1588024/93‑1 (MQ=255)

             aTCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACa                                                                                <  1:1266863/93‑1 (MQ=255)

              tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAAcnn                                                                               >  2:2813061/1‑91 (MQ=255)

              tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaa                                                                               >  1:2219003/1‑93 (MQ=255)

              tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaa                                                                               <  1:3193136/93‑1 (MQ=255)

              tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaa                                                                               >  1:1055521/1‑93 (MQ=255)

              tCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACaa                                                                               >  2:6673621/1‑93 (MQ=255)

               cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaaa                                                                              <  2:4870045/93‑1 (MQ=255)

               cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaaa                                                                              <  2:4687104/93‑1 (MQ=255)

                 gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGGGCAGTAATAACAAATa                                                                            >  1:810113/1‑93 (MQ=255)

                 gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATa                                                                            <  2:6195289/93‑1 (MQ=255)

                   agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAg                                                                          >  1:4284581/1‑93 (MQ=255)

                   agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAg                                                                          >  2:7392877/1‑93 (MQ=255)

                    gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                         <  1:5879713/93‑1 (MQ=255)

                    gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGt                                                                         <  2:7498221/93‑1 (MQ=255)

                     tgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTg                                                                        >  2:2077188/1‑93 (MQ=255)

                      gtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTga                                                                       <  2:2223440/93‑1 (MQ=255)

                       tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTgag                                                                      <  2:1882132/93‑1 (MQ=255)

                        cTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTgaga                                                                     <  2:686086/93‑1 (MQ=255)

                         tGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGagag                                                                    >  1:2141029/1‑93 (MQ=255)

                          gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGc                                                                   <  2:5686967/93‑1 (MQ=255)

                          gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGc                                                                   <  1:2446824/93‑1 (MQ=255)

                           cGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCt                                                                  <  1:4008464/93‑1 (MQ=255)

                            gTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTg                                                                 <  2:4304538/93‑1 (MQ=255)

                             tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGt                                                                >  1:5905918/1‑93 (MQ=255)

                             tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGt                                                                <  2:6517647/93‑1 (MQ=255)

                             tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGt                                                                >  2:4448789/1‑93 (MQ=255)

                                aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAAc                                                             <  2:1719973/93‑1 (MQ=255)

                                  cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActc                                                           <  2:2436349/93‑1 (MQ=255)

                                     aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCg                                                        >  1:727728/1‑93 (MQ=255)

                                     aaaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCg                                                        >  2:6577185/1‑93 (MQ=255)

                                          ggAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtttt                                                   >  2:49938/1‑93 (MQ=255)

                                           gAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTc                                                  <  2:3063439/93‑1 (MQ=255)

                                            aaaTGACCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCt                                                 >  2:4261867/1‑93 (MQ=255)

                                             aaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCtt                                                <  2:4036270/93‑1 (MQ=255)

                                               tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                              <  2:1222373/93‑1 (MQ=255)

                                               tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                              <  2:53965/93‑1 (MQ=255)

                                               tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                              >  2:601003/1‑93 (MQ=255)

                                               tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                              >  1:5154157/1‑93 (MQ=255)

                                                gATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAtt                                             <  2:4098901/93‑1 (MQ=255)

                                                 aTCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAttt                                            >  2:6072446/1‑93 (MQ=255)

                                                 aTCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGCAACTCTCGCTTTTCTTAttt                                            >  2:7443936/1‑93 (MQ=255)

                                                   cAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTcc                                          <  2:2117810/93‑1 (MQ=255)

                                                    aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTccc                                         <  1:1524517/93‑1 (MQ=255)

                                                       cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTg                                      >  2:2344609/1‑93 (MQ=255)

                                                        ggTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGc                                     >  1:7105413/1‑93 (MQ=255)

                                                        ggTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGc                                     >  2:3254665/1‑93 (MQ=255)

                                                        ggTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGc                                     >  1:3591748/1‑93 (MQ=255)

                                                         gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCa                                    <  2:632626/93‑1 (MQ=255)

                                                         gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCa                                    >  1:673945/1‑93 (MQ=255)

                                                         gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCa                                    >  1:3493829/1‑93 (MQ=255)

                                                          tGAAAGCCGATTGGGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGAGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCAt                                   >  1:5687724/1‑93 (MQ=255)

                                                            aaaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaa                                 <  1:1809876/93‑1 (MQ=255)

                                                             aaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaa                                >  1:2366068/1‑93 (MQ=255)

                                                             aaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaa                                >  2:1151029/1‑93 (MQ=255)

                                                              aGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaaa                               >  1:6581010/1‑93 (MQ=255)

                                                                ccGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaaaaa                             <  1:2664973/93‑1 (MQ=255)

                                                                ccGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaaaaa                             <  1:3951819/93‑1 (MQ=255)

                                                                 cGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAg                            <  1:2319586/93‑1 (MQ=255)

                                                                  gATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGc                           >  1:4046242/1‑93 (MQ=255)

                                                                  gATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGc                           >  2:35413/1‑93 (MQ=255)

                                                                  gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGc                           >  1:466548/1‑93 (MQ=255)

                                                                   aTTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                          <  2:2601914/93‑1 (MQ=255)

                                                                   aTTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                          >  1:1438525/1‑93 (MQ=255)

                                                                   aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                          >  1:239325/1‑93 (MQ=255)

                                                                    ttGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCa                         <  1:7454870/93‑1 (MQ=255)

                                                                     tgtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                        <  1:2322839/93‑1 (MQ=255)

                                                                     tgtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                        <  2:800274/93‑1 (MQ=255)

                                                                      gtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCTCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                       >  1:4485096/1‑93 (MQ=255)

                                                                      gtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                       <  2:2951073/93‑1 (MQ=255)

                                                                      gtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                       <  1:5025633/93‑1 (MQ=255)

                                                                       tgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAcc                      <  1:6177400/93‑1 (MQ=255)

                                                                        gATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                     <  1:2797631/93‑1 (MQ=255)

                                                                          tAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGc                   >  1:923935/1‑93 (MQ=255)

                                                                          tAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGc                   <  1:7052453/93‑1 (MQ=255)

                                                                           aaaGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                  <  1:895710/93‑1 (MQ=255)

                                                                             aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAgg                <  1:1018828/93‑1 (MQ=255)

                                                                               tCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt              >  2:1660704/1‑93 (MQ=255)

                                                                                  ggCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc           <  2:1058706/93‑1 (MQ=255)

                                                                                  ggCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc           >  1:4942030/1‑93 (MQ=255)

                                                                                  ggCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc           <  2:912414/93‑1 (MQ=255)

                                                                                   gCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt          >  2:3752197/1‑93 (MQ=255)

                                                                                    cAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt         >  1:6393079/1‑93 (MQ=255)

                                                                                     aGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCttt        >  2:4373841/1‑93 (MQ=255)

                                                                                       gAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc      >  2:6477791/1‑93 (MQ=255)

                                                                                       gAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc      >  1:7410753/1‑93 (MQ=255)

                                                                                       gAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc      <  1:6840572/93‑1 (MQ=255)

                                                                                       gAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc      >  1:1898975/1‑93 (MQ=255)

                                                                                        aaaTGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct     <  2:2506017/93‑1 (MQ=255)

                                                                                           tACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt  >  1:6454133/1‑93 (MQ=255)

CGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGT  >  NC_000913/709229‑709412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`