Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 62,312 T→C Q318R (CAG→CGG)  hepA ← putative ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091362,3120TC83.9% 614.1 / 115.4 279Q318R (CAG→CGG) hepAputative ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (102/132);  total (126/153)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 279 total aligned reads displayed.

TTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCG  >  NC_000913/62222‑62401
                                                                                          |                                                                                         
  tttCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTc                                                                                       <  1:919141/93‑1 (MQ=255)
   ttCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTcc                                                                                      >  1:735922/1‑93 (MQ=255)
     cTGCTCTTCAACGAACTGCGCAAAATTGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCgg                                                                                    >  2:290078/1‑93 (MQ=255)
      tGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCggg                                                                                   <  1:49326/93‑1 (MQ=255)
      tGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCggg                                                                                   <  2:4612773/93‑1 (MQ=255)
       gCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCgggg                                                                                  <  2:5252113/93‑1 (MQ=255)
       gCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCgggg                                                                                  <  2:1136545/93‑1 (MQ=255)
       gCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCgggg                                                                                  >  2:6878944/1‑93 (MQ=255)
        ctctTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGt                                                                                 <  1:2968011/93‑1 (MQ=255)
         tctTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTc                                                                                >  1:2644634/1‑93 (MQ=255)
          ctTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTcg                                                                               >  1:704455/1‑93 (MQ=255)
            tCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTcgcg                                                                             >  1:3604145/1‑93 (MQ=255)
             cAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCgg                                                                            >  2:6093089/1‑93 (MQ=255)
              aaCGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGt                                                                           >  2:6267019/1‑93 (MQ=255)
              aaCGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGAAGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCgga                                                                           >  1:3979747/1‑92 (MQ=255)
               aCGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTc                                                                          >  1:416400/1‑93 (MQ=255)
                 gAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcag                                                                        >  2:7531460/1‑93 (MQ=255)
                 gAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcag                                                                        >  2:3014191/1‑93 (MQ=255)
                  aaCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagc                                                                       >  1:2623050/1‑93 (MQ=255)
                  aaCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagc                                                                       >  1:4449502/1‑93 (MQ=255)
                   aCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTcagca                                                                      <  1:3286316/93‑1 (MQ=255)
                   aCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagca                                                                      >  1:5045755/1‑93 (MQ=255)
                   aCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagca                                                                      <  1:6787963/93‑1 (MQ=255)
                     tGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTcagcagc                                                                    <  2:4597555/93‑1 (MQ=255)
                      gcgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagcagca                                                                   <  2:4989527/93‑1 (MQ=255)
                      gcgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagcagca                                                                   <  1:4627711/93‑1 (MQ=255)
                       cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagcagcag                                                                  <  2:2631982/93‑1 (MQ=255)
                       cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTcagcagcag                                                                  <  2:1214877/93‑1 (MQ=255)
                        gcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGACGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGa                                                                 >  1:2181689/1‑93 (MQ=255)
                         cAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGaa                                                                <  1:6498438/93‑1 (MQ=255)
                         cAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGaa                                                                <  2:4226499/93‑1 (MQ=255)
                         cAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGaa                                                                >  2:5035476/1‑93 (MQ=255)
                          aaaaTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAAc                                                               >  2:5660388/1‑93 (MQ=255)
                          aaaaTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAAc                                                               <  2:2563241/93‑1 (MQ=255)
                           aaaTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACg                                                              >  2:4394537/1‑93 (MQ=255)
                            aaTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGGCGCGGTCAGCAGCAGAACGc                                                             >  1:1425795/1‑93 (MQ=255)
                             aTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGcc                                                            <  1:1214647/93‑1 (MQ=255)
                             aTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGcc                                                            <  2:977651/93‑1 (MQ=255)
                              tCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGccc                                                           <  2:4140778/93‑1 (MQ=255)
                              tCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGccc                                                           >  1:2575535/1‑93 (MQ=255)
                               cGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCg                                                          >  1:4217579/1‑93 (MQ=255)
                                 tGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGc                                                        >  2:2063483/1‑93 (MQ=255)
                                  ggAACCGGTTCGGGTCCAGCAGCCGCGGCGGGGCGAAGTGGCTTTCCACCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGACCGCCCGGCa                                                       <  1:2388123/93‑1 (MQ=255)
                                     aaCGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGt                                                    >  2:100062/1‑93 (MQ=255)
                                       cGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGc                                                  <  2:4047001/93‑1 (MQ=255)
                                        ggTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGct                                                 <  1:7238657/93‑1 (MQ=255)
                                        ggTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGct                                                 <  2:5392914/93‑1 (MQ=255)
                                         gTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctc                                                >  1:4706600/1‑93 (MQ=255)
                                         gTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctc                                                <  2:1598831/93‑1 (MQ=255)
                                         gTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctc                                                <  1:1488127/93‑1 (MQ=255)
                                         gTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctc                                                >  1:1029070/1‑93 (MQ=255)
                                         gTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctc                                                >  2:4768668/1‑93 (MQ=255)
                                          ttCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGctct                                               >  1:5957717/1‑93 (MQ=255)
                                            cGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGc                                             <  2:1970598/93‑1 (MQ=255)
                                               gTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAg                                          >  1:6338210/1‑93 (MQ=255)
                                                tCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgt                                         <  2:4511942/93‑1 (MQ=255)
                                                 ccAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgtt                                        <  2:7226438/93‑1 (MQ=255)
                                                 ccAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgtt                                        <  2:3087145/93‑1 (MQ=255)
                                                  cagcagACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgttg                                       >  1:2263354/1‑93 (MQ=255)
                                                  cagcagACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgttg                                       >  1:6283028/1‑93 (MQ=255)
                                                   agcagACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgttgt                                      >  2:6339618/1‑93 (MQ=255)
                                                    gcagacgcagacGGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAgttgtt                                     >  1:2845217/1‑93 (MQ=255)
                                                     cagacgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTc                                    >  1:4331311/1‑93 (MQ=255)
                                                      agacgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCa                                   >  1:5488389/1‑93 (MQ=255)
                                                      agacgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCa                                   >  1:1792745/1‑93 (MQ=255)
                                                      agacgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCa                                   <  2:3302511/93‑1 (MQ=255)
                                                       gacgcagacgGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCaa                                  <  2:6176416/93‑1 (MQ=255)
                                                        acgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAAt                                 <  1:6917470/93‑1 (MQ=255)
                                                         cgcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATg                                <  2:1542684/93‑1 (MQ=255)
                                                          gcagacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATgg                               >  1:1949517/1‑93 (MQ=255)
                                                            agacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGcc                             <  2:2752999/93‑1 (MQ=255)
                                                            agacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGcc                             <  2:7370660/93‑1 (MQ=255)
                                                             gacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCt                            <  2:6464583/93‑1 (MQ=255)
                                                             gacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCt                            <  2:3179023/93‑1 (MQ=255)
                                                             gacgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGCCCt                            <  1:6971626/93‑1 (MQ=255)
                                                               cgGGCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGa                          >  1:3483676/1‑93 (MQ=255)
                                                                 ggCGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGata                        >  2:4680090/1‑93 (MQ=255)
                                                                   cGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATAtt                      <  1:6079912/93‑1 (MQ=255)
                                                                   cGAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATAtt                      <  2:5361976/93‑1 (MQ=255)
                                                                    gAAGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTc                     <  2:3904245/93‑1 (MQ=255)
                                                                     aaGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCa                    >  1:4760146/1‑93 (MQ=255)
                                                                      aGTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCAc                   >  2:5991239/1‑93 (MQ=255)
                                                                       gTGGCTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACg                  <  2:728490/93‑1 (MQ=255)
                                                                           cTTTCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCtt              >  1:1462160/1‑93 (MQ=255)
                                                                            tttCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTg             <  2:2140871/93‑1 (MQ=255)
                                                                             ttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTgg            >  2:5601278/1‑93 (MQ=255)
                                                                             ttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTgg            <  2:2474411/93‑1 (MQ=255)
                                                                              tCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGgc           <  1:2285545/93‑1 (MQ=255)
                                                                              tCCATCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGgc           >  1:5315322/1‑93 (MQ=255)
                                                                                 aTCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcgcc        >  1:6164965/1‑92 (MQ=255)
                                                                                  tCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCAt       >  1:749324/1‑93 (MQ=255)
                                                                                  tCCCCAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCAt       >  2:6992707/1‑93 (MQ=255)
                                                                                   ccccAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCAta      <  1:3032359/93‑2 (MQ=255)
                                                                                   ccccAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATc      <  2:227352/93‑1 (MQ=255)
                                                                                    cccAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCt     >  2:3559064/1‑93 (MQ=255)
                                                                                    cccAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCt     >  1:1073604/1‑93 (MQ=255)
                                                                                      cAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTc   <  1:866203/93‑1 (MQ=255)
                                                                                      cAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTc   >  1:2765932/1‑93 (MQ=255)
                                                                                      cAGCCGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTc   <  1:565897/93‑1 (MQ=255)
                                                                                      cAGCCGTTCCGGGGCCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTc   <  2:1298843/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCG  >  NC_000913/62222‑62401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑