BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	898,377	A→G	T213A (ACT→GCT)	ybjF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	898,377	0	A	G	83.1%	430.0 / 76.5	196	T213A (ACT→GCT)	ybjF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (13/20); new base G (79/83); total (93/103)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 196 total aligned reads displayed.

GAGACGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTGC  >  NC_000913/898287‑898466

gagaCGGAGATCTTCCTGGCCGAACAACAGGCCCTTGCGGAGCGTTTTAATTGCGTTCCGCTGTTGATCCGTCCGCAAAGTTTCTTCCAGGCt                                                                                         <  2:2637391/93‑1 (MQ=255)

  gaCGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTaa                                                                                       <  1:5100938/93‑1 (MQ=255)

  gaCGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTaa                                                                                       <  2:5388391/93‑1 (MQ=255)

  gaCGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTaa                                                                                       >  1:1700878/1‑93 (MQ=255)

    cGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATc                                                                                     <  1:2459145/93‑1 (MQ=255)

     ggAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATcc                                                                                    <  1:7341620/93‑1 (MQ=255)

     ggAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATcc                                                                                    <  1:7513657/93‑1 (MQ=255)

      gagaTCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCcg                                                                                   >  1:5145206/1‑93 (MQ=255)

        gaTCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCcggc                                                                                 >  2:5327389/1‑93 (MQ=255)

        gaTCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCcggc                                                                                 <  2:1343373/93‑1 (MQ=255)

        gaTCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCcggc                                                                                 >  2:6777068/1‑93 (MQ=255)

         aTCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCcggcg                                                                                >  1:85224/1‑93 (MQ=255)

           cTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGt                                                                              <  2:5821389/93‑1 (MQ=255)

            tACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTc                                                                             <  2:3689360/93‑1 (MQ=255)

             aCCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCg                                                                            >  2:7293043/1‑93 (MQ=255)

              ccTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgc                                                                           >  2:5004996/1‑93 (MQ=255)

               cTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgcc                                                                          <  2:4455164/93‑1 (MQ=255)

                tGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgcca                                                                         <  1:7530800/93‑1 (MQ=255)

                 gACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgccag                                                                        >  1:116673/1‑93 (MQ=255)

                 gACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgccag                                                                        >  1:2755414/1‑93 (MQ=255)

                  aCCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgccagc                                                                       <  2:3410000/93‑1 (MQ=255)

                   ccGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCgccagcc                                                                      >  1:4732208/1‑93 (MQ=255)

                   ccGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCgccagcc                                                                      <  1:2001499/93‑1 (MQ=255)

                    cGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCgccagcca                                                                     <  1:5991848/93‑1 (MQ=255)

                     gAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGccagccag                                                                    <  1:3118428/93‑1 (MQ=255)

                       acaacaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGtt                                                                  >  2:5243527/1‑93 (MQ=255)

                        caacaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTg                                                                 <  1:2872453/93‑1 (MQ=255)

                        caacaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTg                                                                 >  1:5338299/1‑93 (MQ=255)

                          acaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTa                                                               <  1:3915231/93‑1 (MQ=255)

                          acaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTa                                                               <  1:5584838/93‑1 (MQ=255)

                          acaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTa                                                               >  2:4692571/1‑93 (MQ=255)

                          acaGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTa                                                               <  2:4081135/93‑1 (MQ=255)

                           caGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTAc                                                              >  2:4925888/1‑93 (MQ=255)

                           caGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTAc                                                              <  1:6701519/93‑1 (MQ=255)

                             ggCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGc                                                            >  2:789118/1‑93 (MQ=255)

                               cACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCa                                                          <  1:4910511/93‑1 (MQ=255)

                                aCTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCAc                                                         <  1:2883945/93‑1 (MQ=255)

                                 cTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCAcc                                                        <  2:3395557/93‑1 (MQ=255)

                                  tGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcg                                                       <  1:1585482/93‑1 (MQ=255)

                                  tGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcg                                                       >  1:4189921/1‑93 (MQ=255)

                                  tGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcg                                                       >  1:3560759/1‑93 (MQ=255)

                                   ggCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgc                                                      >  1:4443331/1‑93 (MQ=255)

                                      ggAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgcgcg                                                   >  2:1682920/1‑93 (MQ=255)

                                       gAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgcgcgc                                                  <  2:1995484/93‑1 (MQ=255)

                                       gAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgcgcgc                                                  >  2:1824790/1‑93 (MQ=255)

                                       gAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgcgcgc                                                  >  2:3443477/1‑93 (MQ=255)

                                        aGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACcgcgcgcg                                                 >  2:3622881/1‑93 (MQ=255)

                                         gCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGa                                                <  2:1612294/93‑1 (MQ=255)

                                         gCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGa                                                <  1:2329838/93‑1 (MQ=255)

                                          cGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGAc                                               <  1:1007759/93‑1 (MQ=255)

                                            ttttAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTg                                             >  1:6515777/1‑93 (MQ=255)

                                             tttAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTgg                                            >  1:3405937/1‑93 (MQ=255)

                                              ttAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTggg                                           <  2:2307197/93‑1 (MQ=255)

                                              ttAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTggg                                           <  2:152490/93‑1 (MQ=255)

                                              ttAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTggg                                           <  1:2085706/93‑1 (MQ=255)

                                                aaTGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTTCGCCACCGCGCGCGACTGGGTa                                         <  1:1454115/93‑1 (MQ=255)

                                                    aCGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGAc                                     <  1:4458339/93‑1 (MQ=255)

                                                    aCGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGAc                                     <  2:7452102/93‑1 (MQ=255)

                                                    aCGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGAc                                     >  2:1503478/1‑93 (MQ=255)

                                                     cGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACa                                    <  1:5892646/93‑1 (MQ=255)

                                                     cGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACa                                    >  2:3020488/1‑93 (MQ=255)

                                                       tACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGCCTGGGTACGACAGc                                  <  2:6443800/93‑1 (MQ=255)

                                                        aCCGCTGTGGATCCGTCCGCAAGGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCt                                 >  1:4881624/1‑93 (MQ=255)

                                                        aCCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCt                                 <  2:7447299/93‑1 (MQ=255)

                                                          cGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGc                               <  2:93994/93‑1 (MQ=255)

                                                          cGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGc                               <  1:4506103/93‑1 (MQ=255)

                                                          cGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGc                               <  2:244090/93‑1 (MQ=255)

                                                          cGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGc                               <  2:1466993/93‑1 (MQ=255)

                                                           gCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGcc                              >  2:2068731/1‑93 (MQ=255)

                                                           gCTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGcc                              >  2:802272/1‑93 (MQ=255)

                                                            cTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCg                             >  1:5658370/1‑93 (MQ=255)

                                                            cTGTGGATCCGTCCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCg                             >  1:2711506/1‑93 (MQ=255)

                                                             tgtgGATCCGTGCGCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCgg                            >  2:4652474/1‑93 (MQ=255)

                                                                     cgtccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGCCTGGGTACGACAGCTGCCGGTTAAACat                    >  1:4623741/1‑93 (MQ=255)

                                                                      gtccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACata                   <  2:4242507/93‑1 (MQ=255)

                                                                      gtccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACata                   >  2:4949923/1‑93 (MQ=255)

                                                                      gtccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACata                   <  2:5086956/93‑1 (MQ=255)

                                                                      gtccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACata                   >  2:7098291/1‑93 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  >  2:4686702/1‑93 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  >  1:787630/1‑93 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  >  1:6324106/1‑93 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  >  2:6855746/1‑93 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  <  2:1060856/93‑1 (MQ=255)

                                                                       tccgCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACatat                  <  1:7123725/93‑1 (MQ=255)

                                                                        ccgCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATAtg                 >  2:910875/1‑93 (MQ=255)

                                                                         cgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATAtgt                <  2:2958754/93‑1 (MQ=255)

                                                                         cgCAAAGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATAtgt                <  2:5695250/93‑1 (MQ=255)

                                                                            aaaGTTTCTTCCAGGCTAATCCGGCGTTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTggg             <  1:5458409/93‑1 (MQ=255)

                                                                            aaaGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTggg             <  1:1298029/93‑1 (MQ=255)

                                                                              aGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGAt           >  1:5804928/1‑93 (MQ=255)

                                                                              aGTTTCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGAt           >  2:2835454/1‑93 (MQ=255)

                                                                                tttCTTCCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCt         >  2:2479053/1‑93 (MQ=255)

                                                                                  tcttcCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGt       >  1:2131726/1‑93 (MQ=255)

                                                                                    ttcCAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTc     >  2:4521994/1‑93 (MQ=255)

                                                                                     tcCAGGCTAATCCGGCGGTCGCCATCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCt    <  2:4787002/93‑1 (MQ=255)

                                                                                      ccAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTg   >  2:652214/1‑93 (MQ=255)

                                                                                      ccAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTg   <  1:7000587/93‑1 (MQ=255)

                                                                                       cAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTGc  <  1:5391127/93‑1 (MQ=255)

                                                                                       cAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTGc  <  2:6882567/93‑1 (MQ=255)

                                                                                       cAGGCTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTGc  >  1:2952921/1‑93 (MQ=255)

GAGACGGAGATCTACCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTAATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTGTTCTGC  >  NC_000913/898287‑898466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`