BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,046,049	T→C	M374V (ATG→GTG)	ymcA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,046,049	0	T	C	79.1%	479.0 / 144.8	254	M374V (ATG→GTG)	ymcA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (26/27); new base C (107/94); total (133/121)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 261 total aligned reads displayed.

TTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGA  >  NC_000913/1045957‑1046140

  gttgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCatat                                                                                           <  1:1614391/93‑1 (MQ=255)

    tgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGc                                                                                         >  1:4487056/1‑93 (MQ=255)

    tgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGc                                                                                         <  1:3689924/93‑1 (MQ=255)

      ttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTc                                                                                       <  1:4580091/93‑1 (MQ=255)

      ttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTc                                                                                       <  2:4572973/93‑1 (MQ=255)

           gTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCt                                                                                  >  1:5199786/1‑93 (MQ=255)

           gTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCt                                                                                  >  2:582849/1‑93 (MQ=255)

             ggCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCttt                                                                                >  2:1378636/1‑93 (MQ=255)

               ctctGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTg                                                                              >  1:6293669/1‑93 (MQ=255)

                tctGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGc                                                                             >  1:4224993/1‑93 (MQ=255)

                 ctGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCg                                                                            >  2:6007290/1‑93 (MQ=255)

                 ctGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCg                                                                            <  1:5681387/93‑1 (MQ=255)

                  tGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGt                                                                           >  2:4007055/1‑93 (MQ=255)

                   gATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTc                                                                          >  2:5418991/1‑93 (MQ=255)

                   gATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTc                                                                          >  2:6951705/1‑93 (MQ=255)

                   gATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTc                                                                          <  1:4813470/93‑1 (MQ=255)

                   gATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTc                                                                          >  2:2183896/1‑93 (MQ=255)

                    aTTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCa                                                                         >  2:12414/1‑93 (MQ=255)

                    aTTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCa                                                                         >  2:2433595/1‑93 (MQ=255)

                     ttGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCAc                                                                        <  1:7494269/93‑1 (MQ=255)

                     ttGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCAc                                                                        >  2:4986096/1‑93 (MQ=255)

                      tGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACg                                                                       <  2:4329449/93‑1 (MQ=255)

                       gACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGc                                                                      <  2:1789632/93‑1 (MQ=255)

                       gACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGc                                                                      <  1:3590675/93‑1 (MQ=255)

                           aGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAt                                                                  <  1:3146632/93‑1 (MQ=255)

                             gcgcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAtct                                                                <  1:6014715/93‑1 (MQ=255)

                             gcgcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAtct                                                                >  2:6408817/1‑93 (MQ=255)

                               gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                              >  2:220638/1‑93 (MQ=255)

                               gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                              <  2:3559442/93‑1 (MQ=255)

                               gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                              >  1:6070430/1‑93 (MQ=255)

                               gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAtcttc                                                              >  1:1208025/1‑93 (MQ=255)

                                cTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAAtcttct                                                             >  1:2740118/1‑93 (MQ=255)

                                 tGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTtctc                                                            >  2:5230417/1‑93 (MQ=255)

                                  gTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCa                                                           <  2:6791927/93‑1 (MQ=255)

                                   tACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCaa                                                          >  1:4331783/1‑93 (MQ=255)

                                    aCCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAAc                                                         <  1:2371122/93‑1 (MQ=255)

                                      cTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGc                                                       >  2:2942992/1‑93 (MQ=255)

                                      cTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGc                                                       <  2:944269/93‑1 (MQ=255)

                                       tGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGcc                                                      >  2:4113576/1‑93 (MQ=255)

                                        gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCt                                                     <  2:3177494/93‑1 (MQ=255)

                                        gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCt                                                     >  1:4885340/1‑93 (MQ=255)

                                        gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCt                                                     >  2:6193178/1‑93 (MQ=255)

                                        gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCt                                                     <  1:7520388/93‑1 (MQ=255)

                                         ccAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCtt                                                    >  2:7092641/1‑93 (MQ=255)

                                         ccAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCtt                                                    <  1:3083514/93‑1 (MQ=255)

                                           aGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGc                                                  >  1:1790932/1‑93 (MQ=255)

                                           aGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGc                                                  <  2:7522241/93‑1 (MQ=255)

                                           aGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGc                                                  >  2:667473/1‑93 (MQ=255)

                                            gCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCg                                                 >  1:2182878/1‑93 (MQ=255)

                                             cTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCgg                                                >  1:5060347/1‑93 (MQ=255)

                                             cTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCgg                                                >  2:2510771/1‑93 (MQ=255)

                                                    cgcAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTa                                         <  2:7193728/93‑1 (MQ=255)

                                                     gcAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAt                                        <  2:2614667/93‑1 (MQ=255)

                                                     gcAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAt                                        >  1:2920749/1‑93 (MQ=255)

                                                      cAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAtt                                       <  2:2894391/93‑1 (MQ=255)

                                                       aGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTc                                      <  2:5513663/93‑1 (MQ=255)

                                                       aGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTc                                      <  2:4399292/93‑1 (MQ=255)

                                                       aGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGGTATTc                                      >  1:7249883/1‑93 (MQ=255)

                                                          ctggctACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCAcc                                   <  2:4939360/93‑1 (MQ=255)

                                                            ggctACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAg                                 >  2:4026698/1‑93 (MQ=255)

                                                             gctACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGa                                >  1:4826988/1‑93 (MQ=255)

                                                             gctACGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGa                                >  1:1834060/1‑93 (MQ=255)

                                                                aCGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATc                             <  1:6921675/93‑1 (MQ=255)

                                                                 cGTCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATcc                            >  1:6230632/1‑93 (MQ=255)

                                                                   tCGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCgg                          >  1:6110819/1‑93 (MQ=255)

                                                                    cGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGt                         <  2:1008305/93‑1 (MQ=255)

                                                                    cGGTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGt                         <  1:1563965/93‑1 (MQ=255)

                                                                      gTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTg                       >  1:2840619/1‑93 (MQ=255)

                                                                      gTTTCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTg                       <  1:494683/93‑1 (MQ=255)

                                                                       tttCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTgg                      >  2:6242789/1‑93 (MQ=255)

                                                                       tttCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTgg                      <  2:233661/93‑1 (MQ=255)

                                                                       tttCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTgg                      <  2:3728654/93‑1 (MQ=255)

                                                                       tttCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTgg                      >  2:2311526/1‑93 (MQ=255)

                                                                         tCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCa                    >  2:3393279/1‑93 (MQ=255)

                                                                         tCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCa                    <  2:1085618/93‑1 (MQ=255)

                                                                         tCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCa                    <  2:1874910/93‑1 (MQ=255)

                                                                         tCGGTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCa                    <  2:2071790/93‑1 (MQ=255)

                                                                          cGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCAc                   <  2:771376/93‑1 (MQ=255)

                                                                            gTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGa                 >  1:6728144/1‑93 (MQ=255)

                                                                            gTAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGa                 >  2:5775623/1‑93 (MQ=255)

                                                                             tAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGAt                <  2:4155874/93‑1 (MQ=255)

                                                                             tAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGAt                >  1:6087823/1‑93 (MQ=255)

                                                                             tAGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGAt                <  1:401667/93‑1 (MQ=255)

                                                                              aGTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATc               <  1:2633814/93‑1 (MQ=255)

                                                                               gTCACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCg              >  1:1017792/1‑93 (MQ=255)

                                                                                 cACCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAc            <  2:6539074/93‑1 (MQ=255)

                                                                                  aCCATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGAcc           <  2:6334211/93‑1 (MQ=255)

                                                                                   ccATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCg          >  1:5443184/1‑93 (MQ=255)

                                                                                   ccATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCg          >  1:7389981/1‑93 (MQ=255)

                                                                                    cATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGc         >  1:1883550/1‑93 (MQ=255)

                                                                                    cATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGc         >  2:3094996/1‑93 (MQ=255)

                                                                                    cATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGc         <  1:2855942/93‑1 (MQ=255)

                                                                                    cATCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGc         >  2:1715467/1‑93 (MQ=255)

                                                                                     aTCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCt        >  1:6514419/1‑93 (MQ=255)

                                                                                      tCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCtt       >  2:4105296/1‑93 (MQ=255)

                                                                                      tCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCtt       <  1:5485421/93‑1 (MQ=255)

                                                                                      tCGCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCtt       >  2:2737294/1‑93 (MQ=255)

                                                                                       cGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTc      <  1:2062836/93‑1 (MQ=255)

                                                                                        gCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCa     >  1:7212500/1‑93 (MQ=255)

                                                                                        gCCACATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCa     <  1:6494108/93‑1 (MQ=255)

TTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCACCAGAATCCGGTTGGCACGATCGACCGCTTCACGA  >  NC_000913/1045957‑1046140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`