BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,053,820	T→C	T518A (ACC→GCC)	torS ←	TorS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,053,820	0	T	C	89.2%	609.1 / 53.4	232	T518A (ACC→GCC)	torS	TorS
Reads supporting (aligned to +/- strand): ref base T (12/13); new base C (103/104); total (115/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 233 total aligned reads displayed.

ATCGCCCATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCGAGAATAT  >  NC_000913/1053728‑1053909

   gCCCATTAACGCGCACGGCATATCATCTGCAATTGCCGTTGCCAGTCGAATCGGGCGACCTTTCACGCGCCCGCTCATTGATTGCAGGGCACt                                                                                        >  1:5958338/1‑93 (MQ=255)

   gCCCATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGTACt                                                                                        >  1:5064471/1‑93 (MQ=255)

    cccATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACtt                                                                                       >  2:1796293/1‑93 (MQ=255)

    cccATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACtt                                                                                       >  1:2209451/1‑93 (MQ=255)

     ccATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACttt                                                                                      <  2:2480315/93‑1 (MQ=255)

      cATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTc                                                                                     <  1:5123878/93‑1 (MQ=255)

       aTTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTcc                                                                                    <  2:6235155/93‑1 (MQ=255)

       aTTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTcc                                                                                    >  2:1022019/1‑93 (MQ=255)

             gcgcACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCagcagc                                                                              <  1:1031531/93‑1 (MQ=255)

               gcACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCgg                                                                            <  1:5348188/93‑1 (MQ=255)

                cACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCggg                                                                           <  2:2089832/93‑1 (MQ=255)

                 aCGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGgc                                                                          <  1:2546957/93‑1 (MQ=255)

                 aCGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGgc                                                                          <  2:6460483/93‑1 (MQ=255)

                  cGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGgcg                                                                         >  1:6572357/1‑93 (MQ=255)

                   ggCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGgcgc                                                                        >  1:6650304/1‑93 (MQ=255)

                    gCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGcgcg                                                                       <  1:3210645/93‑1 (MQ=255)

                     cATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCgg                                                                      >  1:4876374/1‑93 (MQ=255)

                      atatCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGTAGCGGGCGCGGt                                                                     <  1:4754394/93‑1 (MQ=255)

                      atatCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGt                                                                     <  2:5112990/93‑1 (MQ=255)

                       tatCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGtt                                                                    >  2:1118378/1‑93 (MQ=255)

                        atcatcGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTc                                                                   >  2:7122938/1‑93 (MQ=255)

                         tcatcGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCa                                                                  >  2:38644/1‑93 (MQ=255)

                           atcGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCaaa                                                                <  2:7482471/93‑1 (MQ=255)

                           atcGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCaaa                                                                <  1:133895/93‑1 (MQ=255)

                            tcGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAg                                                               <  1:262529/93‑1 (MQ=255)

                             cGGCAATTGCCGTTTCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAgg                                                              <  2:4583869/93‑1 (MQ=255)

                              ggCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAggg                                                             <  1:3291822/93‑1 (MQ=255)

                              ggCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAggg                                                             >  1:4643517/1‑93 (MQ=255)

                               gCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGc                                                            <  2:2825464/93‑1 (MQ=255)

                               gCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGc                                                            >  1:2428439/1‑93 (MQ=255)

                                 aaTTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTc                                                          >  1:2302928/1‑93 (MQ=255)

                                  aTTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGGAGCGGGCGCGGTTCAAAGGGCTCa                                                         >  2:4790255/1‑93 (MQ=255)

                                  aTTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCa                                                         >  2:4623668/1‑93 (MQ=255)

                                  aTTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCa                                                         >  1:2154261/1‑93 (MQ=255)

                                  aTTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCa                                                         <  1:6834614/93‑1 (MQ=255)

                                   ttGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCAt                                                        <  2:2512729/93‑1 (MQ=255)

                                   ttGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCAt                                                        >  2:1294049/1‑93 (MQ=255)

                                     gCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCg                                                      >  2:4823381/1‑93 (MQ=255)

                                      ccGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGc                                                     <  2:3931198/93‑1 (MQ=255)

                                      ccGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGc                                                     <  1:1647233/93‑1 (MQ=255)

                                      ccGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGc                                                     >  1:1406067/1‑93 (MQ=255)

                                       cGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCt                                                    >  1:6317574/1‑93 (MQ=255)

                                       cGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCt                                                    <  2:3205090/93‑1 (MQ=255)

                                        gTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTg                                                   <  1:5677698/93‑1 (MQ=255)

                                        gTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTg                                                   >  2:5808370/1‑93 (MQ=255)

                                         ttGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGa                                                  >  1:761079/1‑93 (MQ=255)

                                          tGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGAc                                                 >  1:4472242/1‑93 (MQ=255)

                                          tGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGAc                                                 <  1:7480280/93‑1 (MQ=255)

                                           gCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGAcc                                                >  1:2834444/1‑93 (MQ=255)

                                              aGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGaa                                             >  2:3674201/1‑93 (MQ=255)

                                               ggCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGaaa                                            <  2:5914456/93‑1 (MQ=255)

                                                gCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAAc                                           <  2:3002869/93‑1 (MQ=255)

                                                 cGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACa                                          <  2:7456774/93‑1 (MQ=255)

                                                  gAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACAt                                         <  2:2744918/93‑1 (MQ=255)

                                                  gAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACAt                                         >  1:557458/1‑93 (MQ=255)

                                                   aaTCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACAtt                                        >  2:5464614/1‑93 (MQ=255)

                                                   aaTCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACAtt                                        >  2:3500422/1‑93 (MQ=255)

                                                    aTCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTc                                       >  2:3421016/1‑93 (MQ=255)

                                                    aTCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTc                                       >  2:4157004/1‑93 (MQ=255)

                                                    aTCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTc                                       >  2:3886020/1‑93 (MQ=255)

                                                     tCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCt                                      <  1:5303521/93‑1 (MQ=255)

                                                     tCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCt                                      <  2:2729061/93‑1 (MQ=255)

                                                        ggCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGc                                   <  2:424275/93‑1 (MQ=255)

                                                        ggCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGc                                   >  2:6195088/1‑93 (MQ=255)

                                                          cGACCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCa                                 <  1:7072170/93‑1 (MQ=255)

                                                            aCCTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCAcc                               >  1:5805310/1‑93 (MQ=255)

                                                             ccTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCt                              >  2:4102089/1‑93 (MQ=255)

                                                              cTTTCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGCGCTCATCGCTGACCGAAACATTCTTGCCACCTg                             >  2:273864/1‑93 (MQ=255)

                                                               tttCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGc                            >  2:6938922/1‑93 (MQ=255)

                                                                ttCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCt                           <  1:6113988/93‑1 (MQ=255)

                                                                 tCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCtt                          >  1:4010376/1‑93 (MQ=255)

                                                                 tCACCCGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCtt                          <  2:536564/93‑1 (MQ=255)

                                                                    cccGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGa                       >  2:354824/1‑93 (MQ=255)

                                                                      cGTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATa                     <  1:366379/93‑1 (MQ=255)

                                                                       gTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAg                    <  1:3327953/93‑1 (MQ=255)

                                                                       gTCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAg                    >  2:6675335/1‑93 (MQ=255)

                                                                        tCCGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGc                   <  2:3256227/93‑1 (MQ=255)

                                                                         ccGCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCg                  <  2:1543706/93‑1 (MQ=255)

                                                                           gCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGa                >  2:6698738/1‑93 (MQ=255)

                                                                           gCTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGa                >  2:2900532/1‑93 (MQ=255)

                                                                            cTCATTAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaa               <  1:1448322/93‑1 (MQ=255)

                                                                            cTCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaa               <  1:5743892/93‑1 (MQ=255)

                                                                             tCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTGATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaat              <  1:3046628/93‑1 (MQ=255)

                                                                             tCATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaat              <  1:42507/93‑1 (MQ=255)

                                                                              cATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaata             >  1:1903501/1‑93 (MQ=255)

                                                                              cATTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaata             >  2:5554005/1‑93 (MQ=255)

                                                                               aTTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaataa            <  1:3781713/93‑1 (MQ=255)

                                                                               aTTAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaataa            <  2:6001410/93‑1 (MQ=255)

                                                                                ttAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaataat           >  2:7273516/1‑93 (MQ=255)

                                                                                ttAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGaataat           >  2:6176292/1‑93 (MQ=255)

                                                                                 tAATTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATc          >  2:823169/1‑93 (MQ=255)

                                                                                  aaTTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCg         <  1:729986/93‑1 (MQ=255)

                                                                                  aaTTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCg         >  1:5476785/1‑93 (MQ=255)

                                                                                   aTTGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCga        <  2:1581944/93‑1 (MQ=255)

                                                                                    ttGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATagag       >  2:4891924/1‑93 (MQ=255)

                                                                                    ttGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCgag       >  1:5238347/1‑93 (MQ=255)

                                                                                    ttGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCgag       <  1:4109138/93‑1 (MQ=255)

                                                                                     tGCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCgaga      >  1:4554376/1‑93 (MQ=255)

                                                                                      gCAGGGCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCGAGaa     <  1:1727556/93‑1 (MQ=255)

                                                                                         gggCACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATCCTTGCCACCTGCTTCGATAGCGGAATAATCGAGAatat  >  1:1287702/1‑93 (MQ=255)

ATCGCCCATTAACGCGCACGGCATATCATCGGCAATTGCCGTTGCCAGGCGAATCGGGCGACCTTTCACCCGTCCGCTCATTAATTGCAGGGTACTTTCCAGCAGCGGGCGCGGTTCAAAGGGCTCATCGCTGACCGAAACATTCTTGCCACCTGCTTCGATAGCGGAATAATCGAGAATAT  >  NC_000913/1053728‑1053909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`