BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,074,187	T→C	T1307A (ACC→GCC)	putA ←	proline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,187	0	T	C	80.4%	494.4 / 119.1	246	T1307A (ACC→GCC)	putA	proline dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (20/28); new base C (84/113); total (105/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 250 total aligned reads displayed.

ACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCA  >  NC_000913/1074097‑1074278

aCGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTAt                                                                                           <  2:4697816/93‑1 (MQ=255)

aCGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCAt                                                                                           >  2:1654575/1‑93 (MQ=255)

  gTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTc                                                                                         <  1:5456888/93‑1 (MQ=255)

  gTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTc                                                                                         <  1:535603/93‑1 (MQ=255)

   ttAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTca                                                                                        >  2:1122171/1‑93 (MQ=255)

   ttAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTca                                                                                        >  1:6981841/1‑93 (MQ=255)

   ttAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTca                                                                                        <  2:426794/93‑1 (MQ=255)

     aaaGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTcaca                                                                                      >  2:6827733/1‑93 (MQ=255)

     aaaGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTcaca                                                                                      <  2:2461668/93‑1 (MQ=255)

     aaaGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTcaca                                                                                      >  2:5125700/1‑93 (MQ=255)

      aaGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCacac                                                                                     <  2:982086/93‑1 (MQ=255)

      aaGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCacac                                                                                     >  2:1206874/1‑93 (MQ=255)

        gATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTc                                                                                   >  1:5703827/1‑93 (MQ=255)

         aTGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCa                                                                                  <  2:6135805/93‑1 (MQ=255)

         aTGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCa                                                                                  <  1:5975569/93‑1 (MQ=255)

          tGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAg                                                                                 >  1:7439148/1‑93 (MQ=255)

              ggaggaGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGaa                                                                             <  2:3893317/93‑1 (MQ=255)

               gaggagGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAAc                                                                            >  2:4400821/1‑93 (MQ=255)

                 ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGc                                                                          >  2:5260173/1‑93 (MQ=255)

                 ggaggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGc                                                                          >  1:2734334/1‑93 (MQ=255)

                   aggTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTc                                                                        >  2:1544316/1‑93 (MQ=255)

                        gTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGatat                                                                   <  2:5389956/93‑1 (MQ=255)

                         tAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGAtata                                                                  >  2:4516383/1‑93 (MQ=255)

                         tAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGAtata                                                                  >  2:5684896/1‑93 (MQ=255)

                          aaCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATAc                                                                 >  2:6258082/1‑93 (MQ=255)

                           aCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACa                                                                <  2:3303303/93‑1 (MQ=255)

                           aCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACa                                                                <  1:4440863/93‑1 (MQ=255)

                           aCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACa                                                                <  2:631354/93‑1 (MQ=255)

                           aCATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACa                                                                <  2:1353707/93‑1 (MQ=255)

                            cATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAg                                                               <  1:995112/93‑1 (MQ=255)

                             aTCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGc                                                              <  2:2105190/93‑1 (MQ=255)

                              tcctccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGcc                                                             <  2:551052/93‑1 (MQ=255)

                              tcctccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGcc                                                             >  1:6774443/1‑93 (MQ=255)

                              tcctccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGcc                                                             >  1:1360950/1‑93 (MQ=255)

                               cctccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCg                                                            <  2:6739972/93‑1 (MQ=255)

                                 tccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGtt                                                          >  1:5783218/1‑93 (MQ=255)

                                  ccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTc                                                         <  1:1021093/93‑1 (MQ=255)

                                  ccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTc                                                         <  1:1498576/93‑1 (MQ=255)

                                  ccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTc                                                         <  2:7238248/93‑1 (MQ=255)

                                  ccGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTc                                                         <  1:3103651/93‑1 (MQ=255)

                                   cGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTcc                                                        >  2:1931824/1‑93 (MQ=255)

                                    ggCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCa                                                       >  2:6420766/1‑93 (MQ=255)

                                     gCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAg                                                      >  1:4743869/1‑93 (MQ=255)

                                     gCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAg                                                      <  2:2858346/93‑1 (MQ=255)

                                      cTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGa                                                     <  1:3298017/93‑1 (MQ=255)

                                       tACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa                                                    <  2:6946886/93‑1 (MQ=255)

                                       tACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa                                                    <  1:3510043/93‑1 (MQ=255)

                                       tACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGaa                                                    >  2:3941290/1‑93 (MQ=255)

                                        aCCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAg                                                   >  1:3669402/1‑93 (MQ=255)

                                        aCCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAg                                                   <  2:4453354/93‑1 (MQ=255)

                                         ccTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAgg                                                  >  2:1975724/1‑93 (MQ=255)

                                           tGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGat                                                >  2:2240889/1‑93 (MQ=255)

                                            gTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGata                                               <  1:6418346/93‑1 (MQ=255)

                                            gTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGata                                               <  1:1103338/93‑1 (MQ=255)

                                             tttAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGatat                                              <  2:2358516/93‑1 (MQ=255)

                                              ttAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATAtt                                             >  2:4972151/1‑93 (MQ=255)

                                               tAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTg                                            >  2:2571665/1‑93 (MQ=255)

                                               tAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTg                                            >  1:835042/1‑93 (MQ=255)

                                                aaCCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGc                                           <  1:7251542/93‑1 (MQ=255)

                                                 aCCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCt                                          <  1:4591408/93‑1 (MQ=255)

                                                  ccTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCtt                                         >  1:4891436/1‑93 (MQ=255)

                                                  ccTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCtt                                         <  2:5744687/93‑1 (MQ=255)

                                                  ccTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCtt                                         <  1:4009717/93‑1 (MQ=255)

                                                   cTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCttt                                        <  2:4568104/93‑1 (MQ=255)

                                                    tataGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                                       <  2:3799076/93‑1 (MQ=255)

                                                    tataGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                                       <  1:2188978/93‑1 (MQ=255)

                                                    tataGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                                       <  2:927323/93‑1 (MQ=255)

                                                    tataGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTc                                       <  1:7459139/93‑1 (MQ=255)

                                                      taGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGc                                     >  2:3229874/1‑93 (MQ=255)

                                                      taGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGc                                     >  2:2760876/1‑93 (MQ=255)

                                                       aGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGcc                                    <  2:4743191/93‑1 (MQ=255)

                                                         tCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCAc                                  >  1:3815424/1‑93 (MQ=255)

                                                          cATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                                 >  1:6878295/1‑93 (MQ=255)

                                                          cATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                                 >  1:7157163/1‑93 (MQ=255)

                                                          cATTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACg                                 >  1:1638950/1‑93 (MQ=255)

                                                           aTTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACgg                                <  1:6188846/93‑1 (MQ=255)

                                                           aTTAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACgg                                >  1:6922738/1‑93 (MQ=255)

                                                            ttAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACggg                               >  2:3533471/1‑93 (MQ=255)

                                                            ttAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACggg                               >  2:2986386/1‑93 (MQ=255)

                                                            ttAAGCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACggg                               <  1:6057305/93‑1 (MQ=255)

                                                                gCTGGCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCaaa                           <  2:5604540/93‑1 (MQ=255)

                                                                    gCGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAAccc                       <  1:6028894/93‑1 (MQ=255)

                                                                     cGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt                      <  1:3538077/93‑1 (MQ=255)

                                                                     cGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt                      <  1:4291439/93‑1 (MQ=255)

                                                                     cGTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCt                      <  2:2724515/93‑1 (MQ=255)

                                                                      gTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg                     >  1:3708010/1‑93 (MQ=255)

                                                                      gTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg                     <  1:771342/93‑1 (MQ=255)

                                                                      gTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg                     <  2:3108573/93‑1 (MQ=255)

                                                                      gTTACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTg                     <  1:6822379/93‑1 (MQ=255)

                                                                       ttACCGCCAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGc                    >  2:5975048/1‑93 (MQ=255)

                                                                         cccGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCAc                  <  1:5161357/92‑1 (MQ=255)

                                                                             ccAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaa              <  2:7070722/93‑1 (MQ=255)

                                                                             ccAGCGGCAGCGGCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaa              >  2:7498253/1‑93 (MQ=255)

                                                                              cagcggcagcggTATACACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaaa             <  1:2084774/93‑1 (MQ=255)

                                                                              cagcggcagcggCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGaaa             <  1:2473361/93‑1 (MQ=255)

                                                                               agcggcagcggcATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAAc            <  2:4316137/93‑1 (MQ=255)

                                                                                 cggcagcggCATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACaa          <  2:6679771/93‑1 (MQ=255)

                                                                                  ggcagcggTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAAt         <  1:3344111/93‑1 (MQ=255)

                                                                                       cggTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGc    <  2:4035198/93‑1 (MQ=255)

                                                                                         gTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCa  >  2:2636743/1‑93 (MQ=255)

ACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAACGCTCGATATACAGCCGTTCCAGAAGGATATTGCTTTCGCCACGGGCAAAACCCTGCACCGAAACAATTGTGCCA  >  NC_000913/1074097‑1074278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`