BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,086,330	T→C	Y247C (TAC→TGC)	ycdQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,086,330	0	T	C	85.9%	552.6 / 81.1	234	Y247C (TAC→TGC)	ycdQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (107/94); total (125/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 234 total aligned reads displayed.

AGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTG  >  NC_000913/1086240‑1086419

 gTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAAc                                                                                        <  1:2497967/93‑1 (MQ=255)

  tGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAAcc                                                                                       <  1:6256985/93‑1 (MQ=255)

    cccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCa                                                                                     >  2:929307/1‑93 (MQ=255)

    cccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCa                                                                                     <  1:1233827/93‑1 (MQ=255)

    cccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCa                                                                                     <  1:1619695/93‑1 (MQ=255)

    cccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCa                                                                                     <  1:4698408/93‑1 (MQ=255)

     ccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCAc                                                                                    >  2:862541/1‑93 (MQ=255)

     ccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCAc                                                                                    <  2:3146465/93‑1 (MQ=255)

     ccGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCAc                                                                                    >  2:3386302/1‑93 (MQ=255)

      cGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACt                                                                                   >  2:2841380/1‑93 (MQ=255)

      cGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACt                                                                                   <  1:7496095/93‑1 (MQ=255)

       gTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACtt                                                                                  >  2:6871141/1‑93 (MQ=255)

        tGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTc                                                                                 >  1:6395740/1‑93 (MQ=255)

        tGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTc                                                                                 <  2:4864190/93‑1 (MQ=255)

         ggCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCt                                                                                <  1:5345385/93‑1 (MQ=255)

           cTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGc                                                                              >  1:6334218/1‑93 (MQ=255)

             cGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCa                                                                            <  1:4095781/93‑1 (MQ=255)

              gTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAg                                                                           <  1:5909769/93‑1 (MQ=255)

              gTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAg                                                                           <  1:5104712/93‑1 (MQ=255)

               tAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAgg                                                                          <  1:6576791/93‑1 (MQ=255)

               tAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAgg                                                                          >  1:6866636/1‑93 (MQ=255)

                aaaaaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAggg                                                                         <  2:745253/93‑1 (MQ=255)

                 aaaaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGgc                                                                        <  2:2742500/93‑1 (MQ=255)

                 aaaaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGgc                                                                        <  2:2254185/93‑1 (MQ=255)

                 aaaaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGgc                                                                        <  2:1160914/93‑1 (MQ=255)

                 aaaaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGgc                                                                        >  2:5728904/1‑93 (MQ=255)

                   aaaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGgcgc                                                                      <  2:4482779/93‑1 (MQ=255)

                    aaTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCGCTTCTGCCAGGGCGCt                                                                     >  1:2793635/1‑93 (MQ=255)

                     aTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTg                                                                    <  1:1124733/93‑1 (MQ=255)

                     aTCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTg                                                                    >  1:4922654/1‑93 (MQ=255)

                        gTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGcga                                                                 >  1:6510928/1‑93 (MQ=255)

                         tCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGcgac                                                                >  1:4853537/1‑93 (MQ=255)

                          ccACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGcgacg                                                               >  2:1722830/1‑93 (MQ=255)

                            aCTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaa                                                             >  2:7519694/1‑93 (MQ=255)

                            aCTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaa                                                             <  2:5636074/93‑1 (MQ=255)

                             cTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaaa                                                            <  2:6708666/93‑1 (MQ=255)

                             cTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaaa                                                            >  2:6201981/1‑93 (MQ=255)

                             cTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaaa                                                            <  2:5095789/93‑1 (MQ=255)

                             cTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaaa                                                            >  1:5363403/1‑93 (MQ=255)

                             cTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGaaa                                                            >  2:2778354/1‑93 (MQ=255)

                               gATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATg                                                          >  2:5254379/1‑93 (MQ=255)

                               gATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATg                                                          >  1:844957/1‑93 (MQ=255)

                               gATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATg                                                          <  1:5694419/93‑1 (MQ=255)

                               gATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATg                                                          >  1:4320164/1‑93 (MQ=255)

                                aTTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGc                                                         >  1:3756120/1‑93 (MQ=255)

                                 ttCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGCCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCg                                                        <  2:7195630/93‑1 (MQ=255)

                                  tCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCgg                                                       >  1:1842091/1‑93 (MQ=255)

                                   cAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGc                                                      <  2:5712873/93‑1 (MQ=255)

                                    aaCTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCa                                                     >  1:908874/1‑93 (MQ=255)

                                       tGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATa                                                  >  2:1385315/1‑93 (MQ=255)

                                          aGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAaca                                               <  1:4645271/93‑1 (MQ=255)

                                           gCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAacac                                              <  1:3021279/93‑1 (MQ=255)

                                           gCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAacac                                              <  2:1552169/93‑1 (MQ=255)

                                            cTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACAcc                                             <  1:2051806/93‑1 (MQ=255)

                                             ttCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCg                                            <  1:6716820/93‑1 (MQ=255)

                                                cAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGaa                                         >  1:2926553/1‑93 (MQ=255)

                                                 aGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGaaa                                        >  1:4992557/1‑93 (MQ=255)

                                                   cTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACg                                      >  2:544887/1‑93 (MQ=255)

                                                   cTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACg                                      <  2:1041269/93‑1 (MQ=255)

                                                   cTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACg                                      >  1:4802653/1‑93 (MQ=255)

                                                    tAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACgg                                     >  2:2126437/1‑93 (MQ=255)

                                                    tAATATCAATATCTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACgg                                     >  2:5563043/1‑93 (MQ=255)

                                                      atatcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGAGGAAATGCGCCAATAACACGGGAAACGGTa                                   >  1:4751509/1‑93 (MQ=255)

                                                      atatcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTa                                   >  1:3308023/1‑93 (MQ=255)

                                                      atatcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTa                                   >  1:3878577/1‑93 (MQ=255)

                                                       tatcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTaa                                  >  2:2291928/1‑93 (MQ=255)

                                                        atcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTaaa                                 <  1:5548966/93‑1 (MQ=255)

                                                         tcaatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAAt                                >  2:2753881/1‑93 (MQ=255)

                                                         tcaatatcTTCGGTGATCATATCGTAACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAAt                                <  2:6624371/93‑1 (MQ=255)

                                                           aatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATAc                              >  1:7038417/1‑93 (MQ=255)

                                                           aatatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATAc                              >  2:6527025/1‑93 (MQ=255)

                                                            atatcTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACg                             <  2:7414682/93‑1 (MQ=255)

                                                            atatcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACg                             >  2:549230/1‑93 (MQ=255)

                                                              atcTTCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGtt                           <  2:3074094/93‑1 (MQ=255)

                                                                  tCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCa                       <  1:3933812/93‑1 (MQ=255)

                                                                  tCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCa                       >  1:7202661/1‑93 (MQ=255)

                                                                  tCGGTGATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCa                       <  2:706180/93‑1 (MQ=255)

                                                                      tGATCATATCGTCGCTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACCCCGGAAACGGTAAATACGTTTCCATAGa                   <  1:6918078/93‑1 (MQ=255)

                                                                      tGATCATATCGTCACTCCAGTAACCCACTTCTGCCGGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGa                   <  2:2453743/93‑1 (MQ=255)

                                                                       gATCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGAt                  >  2:471016/1‑93 (MQ=255)

                                                                        aTCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATa                 <  1:1021193/93‑1 (MQ=255)

                                                                        aTCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATa                 >  2:6347236/1‑93 (MQ=255)

                                                                        aTCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATa                 >  2:3918735/1‑93 (MQ=255)

                                                                         tCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATAc                <  2:1835679/93‑1 (MQ=255)

                                                                         tCATATCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATAc                <  1:2418123/93‑1 (MQ=255)

                                                                           atatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGc              >  1:1042678/1‑93 (MQ=255)

                                                                           atatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGc              >  1:6135128/1‑93 (MQ=255)

                                                                           atatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGc              <  2:6132083/93‑1 (MQ=255)

                                                                           atatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGc              >  1:3074618/1‑93 (MQ=255)

                                                                            tatCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCt             >  2:4033841/1‑93 (MQ=255)

                                                                            tatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCt             <  1:3682384/93‑1 (MQ=255)

                                                                            tatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCt             >  1:4072961/1‑93 (MQ=255)

                                                                            tatCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCt             >  2:2386741/1‑93 (MQ=255)

                                                                             atCGTCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTg            >  2:3727614/1‑93 (MQ=255)

                                                                                gTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGt         <  1:4445215/93‑1 (MQ=255)

                                                                                 tCACTCCAGCAACCCGCTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGtt        >  1:1434993/1‑93 (MQ=255)

                                                                                 tCACTCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGtt        >  1:3569402/1‑93 (MQ=255)

                                                                                     tCCAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCt    <  2:2659492/93‑1 (MQ=255)

                                                                                      ccAGCAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCtt   >  2:4978491/1‑93 (MQ=255)

                                                                                       cAGTAACCCACTTCTGCCAGGGCGCTACGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTg  >  1:2743170/1‑93 (MQ=255)

AGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCCAGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTG  >  NC_000913/1086240‑1086419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`