BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,101	T→C	M164V (ATG→GTG)	rne ←	RNase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,101	0	T	C	84.5%	464.8 / 80.8	207	M164V (ATG→GTG)	rne	RNase E
Reads supporting (aligned to +/- strand): ref base T (17/15); new base C (97/78); total (114/93)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 209 total aligned reads displayed.

GATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGG  >  NC_000913/1143009‑1143192

gATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCAt                                                                                             <  1:1871189/93‑1 (MQ=255)

gATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCAt                                                                                             <  2:6077002/93‑1 (MQ=255)

 aTGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATg                                                                                            >  2:6237593/1‑93 (MQ=255)

  tGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGc                                                                                           >  2:7497625/1‑93 (MQ=255)

  tGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGc                                                                                           >  2:3397363/1‑93 (MQ=255)

   ggCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGcc                                                                                          >  2:649463/1‑93 (MQ=255)

    gCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCt                                                                                         >  2:3129756/1‑93 (MQ=255)

      ttCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTc                                                                                       >  1:5986681/1‑93 (MQ=255)

       tCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGAGCGCACGATAAGCCCCACGCCTTcc                                                                                      >  2:2633815/1‑93 (MQ=255)

        cccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCg                                                                                     <  1:7256071/93‑1 (MQ=255)

         ccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCgg                                                                                    >  2:7525085/1‑93 (MQ=255)

         ccAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCgg                                                                                    >  1:770905/1‑93 (MQ=255)

          cAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGc                                                                                   >  1:1495844/1‑93 (MQ=255)

          cAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGc                                                                                   >  1:2091325/1‑93 (MQ=255)

           aGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCa                                                                                  <  2:7200239/93‑1 (MQ=255)

           aGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCa                                                                                  <  2:7012812/93‑1 (MQ=255)

             tgtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGt                                                                                <  1:6493605/93‑1 (MQ=255)

              ttttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGtt                                                                               <  2:6798949/92‑1 (MQ=255)

              gtTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGtt                                                                               <  1:3087166/93‑1 (MQ=255)

               tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTc                                                                              <  1:1982164/93‑1 (MQ=255)

               tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTc                                                                              >  1:6804657/1‑93 (MQ=255)

               tttCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTc                                                                              <  1:4083601/93‑1 (MQ=255)

                 tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                            <  1:2180442/93‑1 (MQ=255)

                 tCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCaa                                                                            <  2:1319541/93‑1 (MQ=255)

                  cAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAg                                                                           >  2:4915576/1‑93 (MQ=255)

                  cAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAg                                                                           <  2:7328417/93‑1 (MQ=255)

                  cAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAg                                                                           >  2:5034085/1‑93 (MQ=255)

                  cAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAg                                                                           <  2:741000/93‑1 (MQ=255)

                   aGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAgg                                                                          <  1:3509405/93‑1 (MQ=255)

                     aCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCt                                                                        <  1:1231359/93‑1 (MQ=255)

                     aCGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCt                                                                        <  2:4351994/93‑1 (MQ=255)

                      cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCtt                                                                       <  2:1112559/93‑1 (MQ=255)

                      cGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCtt                                                                       <  2:4885890/93‑1 (MQ=255)

                       ggAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTg                                                                      >  2:5287028/1‑93 (MQ=255)

                        gAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGc                                                                     <  2:6114270/93‑1 (MQ=255)

                         aaGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGcc                                                                    <  2:7142311/93‑1 (MQ=255)

                           gCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAg                                                                  >  2:5255803/1‑93 (MQ=255)

                             ttAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTg                                                                >  2:7385476/1‑93 (MQ=255)

                             ttAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTg                                                                >  2:5956845/1‑93 (MQ=255)

                             ttAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTg                                                                <  1:7385340/93‑1 (MQ=255)

                              tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGc                                                               >  1:4893614/1‑93 (MQ=255)

                              tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGc                                                               >  1:6341577/1‑93 (MQ=255)

                              tAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGc                                                               >  2:6430769/1‑93 (MQ=255)

                               aaaTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGct                                                              >  1:322305/1‑93 (MQ=255)

                                 aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttc                                                            <  2:4644374/93‑1 (MQ=255)

                                 aTCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttc                                                            >  1:6242382/1‑93 (MQ=255)

                                  tCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttct                                                           >  2:3293765/1‑93 (MQ=255)

                                  tCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGcttct                                                           >  2:85007/1‑93 (MQ=255)

                                    ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCttt                                                         <  1:4121654/93‑1 (MQ=255)

                                    ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCttt                                                         <  1:2024808/93‑1 (MQ=255)

                                    ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCttt                                                         >  2:4741720/1‑93 (MQ=255)

                                    ccATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCttt                                                         >  2:1897700/1‑93 (MQ=255)

                                      aTTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTa                                                       >  1:3901877/1‑93 (MQ=255)

                                      aTTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTa                                                       >  2:516822/1‑93 (MQ=255)

                                      aTTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTa                                                       >  2:6291352/1‑93 (MQ=255)

                                       ttGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTaa                                                      >  2:354216/1‑93 (MQ=255)

                                       ttGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTaa                                                      <  2:6678547/93‑1 (MQ=255)

                                         gCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAAtt                                                    >  1:1106065/1‑93 (MQ=255)

                                            gcgcCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCgg                                                 <  1:6591504/93‑1 (MQ=255)

                                               ccTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAc                                              >  2:1519995/1‑93 (MQ=255)

                                                cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                             >  2:6711666/1‑93 (MQ=255)

                                                cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                             >  1:4839892/1‑93 (MQ=255)

                                                cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                             >  2:392123/1‑93 (MQ=255)

                                                cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                             >  1:2929830/1‑93 (MQ=255)

                                                cTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACg                                             >  1:4117032/1‑93 (MQ=255)

                                                 tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                            >  2:1722753/1‑93 (MQ=255)

                                                 tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                            >  2:2177154/1‑93 (MQ=255)

                                                 tCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACgg                                            >  2:2106693/1‑93 (MQ=255)

                                                  cagcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgt                                           <  1:4008135/93‑1 (MQ=255)

                                                  cagcagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgt                                           <  1:1729316/93‑1 (MQ=255)

                                                     cagATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgt                                        <  1:2834528/93‑1 (MQ=255)

                                                      agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                       >  2:444380/1‑93 (MQ=255)

                                                      agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                       >  1:1640557/1‑93 (MQ=255)

                                                      agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                       >  1:600858/1‑93 (MQ=255)

                                                      agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                       <  1:977753/93‑1 (MQ=255)

                                                       gATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGtcgtcg                                      >  2:1376749/1‑93 (MQ=255)

                                                       gATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGtcgtcg                                      >  1:7051316/1‑93 (MQ=255)

                                                           tGCCGACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCtt                                  >  2:376621/1‑93 (MQ=255)

                                                               gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt                              <  2:3650027/93‑1 (MQ=255)

                                                               gACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt                              >  1:1427561/1‑93 (MQ=255)

                                                               gACGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt                              >  2:2375299/1‑93 (MQ=255)

                                                                aCGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATa                             <  1:7134559/93‑1 (MQ=255)

                                                                 cGCCAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATAc                            >  2:4097680/1‑93 (MQ=255)

                                                                   ccAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACgg                          >  2:5460970/1‑93 (MQ=255)

                                                                   ccAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACgg                          >  1:3847867/1‑93 (MQ=255)

                                                                    cAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGc                         <  1:1010230/93‑1 (MQ=255)

                                                                    cAGCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGc                         >  1:2625202/1‑93 (MQ=255)

                                                                      gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCga                       >  1:4735910/1‑93 (MQ=255)

                                                                      gCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCga                       >  1:5632087/1‑93 (MQ=255)

                                                                      gCGGTGCGCACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCga                       <  1:7043639/93‑1 (MQ=255)

                                                                           gcgcACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAAt                  <  1:6689497/93‑1 (MQ=255)

                                                                            cgcACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg                 <  2:6924818/93‑1 (MQ=255)

                                                                             gcACGATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGc                >  1:5217285/1‑93 (MQ=255)

                                                                                 gATAAGGCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAcc            <  1:3279485/93‑1 (MQ=255)

                                                                                 gATAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAcc            <  2:6357025/93‑1 (MQ=255)

                                                                                  aTAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCAccc           >  1:3524048/1‑93 (MQ=255)

                                                                                   tAAGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg          >  1:5234399/1‑93 (MQ=255)

                                                                                     aGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg        >  1:4225086/1‑93 (MQ=255)

                                                                                     aGCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg        >  2:58382/1‑93 (MQ=255)

                                                                                      gCCCCACGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgc       >  1:5454005/1‑93 (MQ=255)

GATGGCTTCCCAGTGTTTCAGACGGAAGCTTAAATCCCATTGCAGCGCCTCAGCAGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGG  >  NC_000913/1143009‑1143192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`