BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,390	A→G	T131A (ACC→GCC)	ycfH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,390	0	A	G	82.1%	584.8 / 104.1	268	T131A (ACC→GCC)	ycfH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (22/26); new base G (120/100); total (142/126)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAG  >  NC_000913/1156300‑1156481

 acacGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGccc                                                                                          >  1:802703/1‑93 (MQ=255)

 acacGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATAccc                                                                                          <  2:4245122/93‑1 (MQ=255)

  cacGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCg                                                                                         >  1:4619480/1‑93 (MQ=255)

        gaaaaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGc                                                                                   <  2:6132482/91‑1 (MQ=255)

         gAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGcc                                                                                  >  2:3363273/1‑93 (MQ=255)

         gAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGcc                                                                                  >  1:1692624/1‑93 (MQ=255)

         gAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGcc                                                                                  <  2:6297801/93‑1 (MQ=255)

         gAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGcc                                                                                  <  2:4531227/93‑1 (MQ=255)

         gAAACTAAAGCACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGcc                                                                                  >  1:5286917/1‑93 (MQ=255)

          aaaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGccc                                                                                 <  1:568312/93‑1 (MQ=255)

          aaaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGccc                                                                                 >  2:4636121/1‑93 (MQ=255)

          aaaCAAAAGTACGTCAGAAAGAGCCCTTCACCCATCATACCCAGATTGGTCGTAAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGccc                                                                                 <  1:6983384/93‑1 (MQ=255)

           aaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCg                                                                                >  1:3942336/1‑93 (MQ=255)

            aCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGt                                                                               <  2:6685847/93‑1 (MQ=255)

            aCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGt                                                                               >  2:1615632/1‑93 (MQ=255)

              tAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGc                                                                             <  2:659366/93‑1 (MQ=255)

              tAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGc                                                                             >  1:4418363/1‑93 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                            >  1:2714418/1‑93 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                            >  1:1313330/1‑93 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                            >  1:4466885/1‑93 (MQ=255)

                aaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCg                                                                           >  1:6641503/1‑93 (MQ=255)

                aaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCg                                                                           >  1:6110178/1‑93 (MQ=255)

                 aGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGa                                                                          >  1:6057593/1‑93 (MQ=255)

                   tACGTCAGCAAGAGTACTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATa                                                                        <  2:5935471/93‑1 (MQ=255)

                       tCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCt                                                                    <  1:4203917/93‑1 (MQ=255)

                       tCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCt                                                                    <  1:3757634/93‑1 (MQ=255)

                       tCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCt                                                                    <  1:3353368/93‑1 (MQ=255)

                          gCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGc                                                                 >  1:5103527/1‑93 (MQ=255)

                            aaGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCaa                                                               >  1:2258886/1‑93 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                              >  2:3914829/1‑93 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                              >  2:3796085/1‑93 (MQ=255)

                               agTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTc                                                            <  1:6885443/93‑1 (MQ=255)

                                 tCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTg                                                          <  2:6924280/93‑1 (MQ=255)

                                  ccTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCNGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgc                                                         <  2:2722719/93‑1 (MQ=255)

                                  ccTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgc                                                         >  2:7513526/1‑93 (MQ=255)

                                  ccTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgc                                                         <  2:4875941/93‑1 (MQ=255)

                                  ccTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgc                                                         <  2:474431/93‑1 (MQ=255)

                                   cTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgcg                                                        >  1:1791954/1‑93 (MQ=255)

                                   cTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgcg                                                        <  2:5342315/93‑1 (MQ=255)

                                   cTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgcg                                                        <  2:4996835/93‑1 (MQ=255)

                                     tCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGcgcg                                                      >  2:3654743/1‑93 (MQ=255)

                                       atccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaa                                                    >  1:4744118/1‑93 (MQ=255)

                                       atccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaa                                                    <  2:233178/93‑1 (MQ=255)

                                        tccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaag                                                   >  2:3317961/1‑93 (MQ=255)

                                          catcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaagaa                                                 >  2:7331376/1‑93 (MQ=255)

                                          catcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaagaa                                                 <  1:7291504/93‑1 (MQ=255)

                                            tcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaa                                               <  2:2077418/93‑1 (MQ=255)

                                            tcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaa                                               >  2:5105865/1‑93 (MQ=255)

                                             catATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaaa                                              >  2:2994433/1‑93 (MQ=255)

                                             catATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaaa                                              <  1:6775933/93‑1 (MQ=255)

                                              atatCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAg                                             <  1:4158935/93‑1 (MQ=255)

                                               tatCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGt                                            <  1:1391382/93‑1 (MQ=255)

                                               tatCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGt                                            >  2:3449480/1‑93 (MQ=255)

                                               tatCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGt                                            >  2:7153027/1‑93 (MQ=255)

                                                 tCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGa                                          <  2:3699840/93‑1 (MQ=255)

                                                  ccAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGAc                                         >  2:6464629/1‑93 (MQ=255)

                                                   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACg                                        <  2:191114/93‑1 (MQ=255)

                                                   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACg                                        <  1:2452349/93‑1 (MQ=255)

                                                    aGATTGGTCGTGAACTGAACAAGCAGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACgg                                       <  1:983051/93‑1 (MQ=255)

                                                     gATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGa                                      >  1:1005970/1‑93 (MQ=255)

                                                     gATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGa                                      <  1:261850/93‑1 (MQ=255)

                                                       ttGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAtt                                    <  1:928631/93‑1 (MQ=255)

                                                       ttGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAtt                                    >  2:112122/1‑93 (MQ=255)

                                                           tCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCgg                                <  2:3143732/93‑1 (MQ=255)

                                                           tCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGAGCGAAGAAAAAGTGACGGATTGCgg                                <  2:1588549/93‑1 (MQ=255)

                                                            cGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGt                               <  2:5037344/93‑1 (MQ=255)

                                                               gaactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGc                            >  1:3853216/1‑93 (MQ=255)

                                                                   tgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGtac                        >  2:16783/1‑93 (MQ=255)

                                                                     aacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGtacta                      >  2:5759641/1‑93 (MQ=255)

                                                                         aGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACt                  <  1:1050492/93‑1 (MQ=255)

                                                                         aGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACt                  <  2:4015493/93‑1 (MQ=255)

                                                                         aGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACt                  >  2:6622728/1‑93 (MQ=255)

                                                                         aGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACt                  >  2:5206028/1‑93 (MQ=255)

                                                                          gCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTg                 >  2:6860963/1‑93 (MQ=255)

                                                                           ccGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt                <  1:3475895/93‑1 (MQ=255)

                                                                           ccGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt                <  1:6657705/93‑1 (MQ=255)

                                                                           ccGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt                <  2:3244162/93‑1 (MQ=255)

                                                                           ccGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt                <  1:1411807/93‑1 (MQ=255)

                                                                            cGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGtt               >  1:830318/1‑93 (MQ=255)

                                                                            cGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGtt               >  1:564424/1‑93 (MQ=255)

                                                                             ggTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGttt              >  1:5462390/1‑93 (MQ=255)

                                                                             ggTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGttt              >  1:7230172/1‑93 (MQ=255)

                                                                             ggTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGttt              <  1:4814593/93‑1 (MQ=255)

                                                                              gTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGtttt             >  1:5315570/1‑93 (MQ=255)

                                                                               ttATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTa            >  2:574669/1‑93 (MQ=255)

                                                                               ttATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTa            >  1:2211659/1‑93 (MQ=255)

                                                                               ttATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTa            >  1:1956380/1‑93 (MQ=255)

                                                                                tATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTAc           <  1:5926322/93‑1 (MQ=255)

                                                                                tATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTAc           <  2:4208280/93‑1 (MQ=255)

                                                                                 aTCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACa          <  2:5506592/93‑1 (MQ=255)

                                                                                 aTCGTCCAAGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACa          >  2:2425447/1‑93 (MQ=255)

                                                                                  tCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACag         <  2:2169221/93‑1 (MQ=255)

                                                                                  tCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACag         <  2:7116478/93‑1 (MQ=255)

                                                                                   cGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACaga        <  1:4990616/93‑1 (MQ=255)

                                                                                   cGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACaga        <  2:4600134/93‑1 (MQ=255)

                                                                                    gTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACagag       <  1:7317373/93‑1 (MQ=255)

                                                                                     tCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAgg      <  2:4418028/93‑1 (MQ=255)

                                                                                     tCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAgg      >  1:1777277/1‑93 (MQ=255)

                                                                                     tCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAgg      >  1:6480540/1‑93 (MQ=255)

                                                                                      ccATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGa     >  2:7214338/1‑93 (MQ=255)

TACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAG  >  NC_000913/1156300‑1156481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`