BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,199,211	T→C	V273V (GTA→GTG)	intE ←	prophage e14 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,199,211	0	T	C	84.4%	595.2 / 101.5	262	V273V (GTA→GTG)	intE	prophage e14 integrase
Reads supporting (aligned to +/- strand): ref base T (23/18); new base C (106/115); total (129/133)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 269 total aligned reads displayed.

GGCTTTAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACATG  >  NC_000913/1199121‑1199300

 gCTTTAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGccact                                                                                        >  2:1352120/1‑93 (MQ=255)

   tttAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCcactac                                                                                      >  1:1590659/1‑93 (MQ=255)

    ttAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACa                                                                                     >  1:761048/1‑93 (MQ=255)

    ttAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACa                                                                                     >  2:643217/1‑93 (MQ=255)

     tAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACAt                                                                                    <  2:4665516/93‑1 (MQ=255)

      aaCCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATc                                                                                   >  1:1445633/1‑93 (MQ=255)

       aCCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATcg                                                                                  >  2:754907/1‑93 (MQ=255)

       aCCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATcg                                                                                  >  2:5771805/1‑93 (MQ=255)

        ccTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATcgc                                                                                 >  1:7434713/1‑93 (MQ=255)

        ccTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATcgc                                                                                 <  2:1563966/93‑1 (MQ=255)

         cTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATcgcg                                                                                >  2:336702/1‑93 (MQ=255)

          tGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGt                                                                               >  2:5609652/1‑93 (MQ=255)

          tGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGt                                                                               <  2:3916045/93‑1 (MQ=255)

          tGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGt                                                                               >  2:5994581/1‑93 (MQ=255)

            cgcTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTaa                                                                             >  2:1461140/1‑93 (MQ=255)

             gcTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTaaa                                                                            >  1:1101205/1‑93 (MQ=255)

             gcTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTaaa                                                                            <  1:7220395/93‑1 (MQ=255)

              cTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAAc                                                                           <  1:1002075/93‑1 (MQ=255)

              cTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAAc                                                                           <  1:5242901/93‑1 (MQ=255)

                  gcgcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCa                                                                       <  1:2785601/93‑1 (MQ=255)

                   cgcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAg                                                                      >  2:3023902/1‑93 (MQ=255)

                   cgcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAg                                                                      <  1:4263454/93‑1 (MQ=255)

                    gcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGt                                                                     >  2:2361802/1‑93 (MQ=255)

                    gcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGt                                                                     >  2:7299569/1‑93 (MQ=255)

                    gcTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGt                                                                     <  2:1977837/93‑1 (MQ=255)

                     ctctGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGtt                                                                    <  2:2565734/93‑1 (MQ=255)

                         gCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATc                                                                >  1:2924408/1‑93 (MQ=255)

                           ttGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGc                                                              >  1:4106102/1‑93 (MQ=255)

                           ttGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGc                                                              >  2:1611143/1‑93 (MQ=255)

                            tgtgAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCa                                                             >  2:4197938/1‑93 (MQ=255)

                            tgtgAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCa                                                             >  2:2828640/1‑93 (MQ=255)

                              tgAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCAtt                                                           >  2:2467470/1‑93 (MQ=255)

                                aaGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGa                                                         <  2:4577288/93‑1 (MQ=255)

                                aaGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGa                                                         <  2:4275559/93‑1 (MQ=255)

                                  gTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAgg                                                       <  2:7039734/93‑1 (MQ=255)

                                  gTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAgg                                                       <  1:235764/93‑1 (MQ=255)

                                   ttGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGc                                                      <  1:2713828/93‑1 (MQ=255)

                                   ttGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGc                                                      <  1:3359269/93‑1 (MQ=255)

                                    tGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCg                                                     >  1:4553696/1‑93 (MQ=255)

                                     gAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGa                                                    >  1:5947705/1‑93 (MQ=255)

                                     gAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGa                                                    >  2:5963855/1‑93 (MQ=255)

                                      aaCGAAAAAAATGCACTAAGTATGGACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGaa                                                   <  1:4857462/93‑1 (MQ=255)

                                      aaCGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGaa                                                   <  2:870636/93‑1 (MQ=255)

                                      aaCGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGaa                                                   <  1:2968717/93‑1 (MQ=255)

                                      aaCGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGaa                                                   <  1:1754574/93‑1 (MQ=255)

                                        cGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAaga                                                 <  1:2533135/93‑1 (MQ=255)

                                        cGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAaga                                                 <  2:360270/93‑1 (MQ=255)

                                         gAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAagag                                                >  1:2675623/1‑93 (MQ=255)

                                         gAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAagag                                                <  2:1756098/93‑1 (MQ=255)

                                         gAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAagag                                                <  1:6379329/93‑1 (MQ=255)

                                           aaaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaa                                              <  2:2301387/93‑1 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             <  1:3918313/93‑1 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             <  2:690104/93‑1 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             >  2:6151569/1‑93 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             <  2:5319665/93‑1 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             <  1:3125907/93‑1 (MQ=255)

                                            aaaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGaaa                                             <  1:6713556/93‑1 (MQ=255)

                                             aaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAag                                            <  2:2076200/93‑1 (MQ=255)

                                             aaaaTGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAag                                            >  1:4638755/1‑93 (MQ=255)

                                                  gCACTAAGTATGCACTGACTGCATAGTCACGGCATCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGaa                                       <  2:6818570/93‑1 (MQ=255)

                                                  gCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGaa                                       <  2:653916/93‑1 (MQ=255)

                                                    aCTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAAtt                                     <  2:1108939/93‑1 (MQ=255)

                                                    aCTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAAtt                                     >  1:5776676/1‑93 (MQ=255)

                                                    aCTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAAtt                                     >  2:3073176/1‑93 (MQ=255)

                                                      tAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGc                                   >  1:6919164/1‑93 (MQ=255)

                                                      tAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGc                                   >  1:6138919/1‑93 (MQ=255)

                                                        aGTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGa                                 >  1:1471211/1‑93 (MQ=255)

                                                         gTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAt                                >  1:4391729/1‑93 (MQ=255)

                                                         gTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAt                                <  1:2277020/93‑1 (MQ=255)

                                                         gTATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAt                                <  1:4807052/93‑1 (MQ=255)

                                                          tATGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAtt                               >  1:1862795/1‑93 (MQ=255)

                                                           aTGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAttt                              <  1:250633/93‑1 (MQ=255)

                                                            tGCACTGACTGCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGAtttt                             <  2:6910953/93‑1 (MQ=255)

                                                                 tgactgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTcc                        <  2:6472344/93‑1 (MQ=255)

                                                                  gactgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTccc                       <  1:1287652/93‑1 (MQ=255)

                                                                  gactgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTccc                       >  2:567233/1‑93 (MQ=255)

                                                                  gactgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTccc                       <  2:3428742/93‑1 (MQ=255)

                                                                   actgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCg                      >  2:667430/1‑93 (MQ=255)

                                                                   actgCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCg                      <  2:2729373/93‑1 (MQ=255)

                                                                      gCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTc                   >  2:4410204/1‑93 (MQ=255)

                                                                      gCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTc                   >  2:5006934/1‑93 (MQ=255)

                                                                      gCATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTc                   >  2:1871913/1‑93 (MQ=255)

                                                                       cATAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCt                  <  2:6230952/93‑1 (MQ=255)

                                                                        aTAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCtt                 >  2:2843791/1‑93 (MQ=255)

                                                                        aTAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCtt                 >  2:2420586/1‑93 (MQ=255)

                                                                         tAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCttt                >  2:5064774/1‑93 (MQ=255)

                                                                         tAGTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCttt                >  1:6577795/1‑93 (MQ=255)

                                                                           gTCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTc              >  1:4169897/1‑93 (MQ=255)

                                                                            tCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTcc             >  2:6615937/1‑93 (MQ=255)

                                                                            tCACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTcc             >  2:411989/1‑93 (MQ=255)

                                                                             cACGGCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCt            <  1:4744930/93‑1 (MQ=255)

                                                                                 gCAGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAAt        <  1:2105998/93‑1 (MQ=255)

                                                                                   aGCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGa      <  1:342246/93‑1 (MQ=255)

                                                                                    gCGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGAc     <  1:5584528/93‑1 (MQ=255)

                                                                                     ggggCCACTCCATCGCGT‑AACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACAt   <  1:1805261/92‑1 (MQ=255)

                                                                                     cGGGCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACa    <  2:7153921/93‑1 (MQ=255)

                                                                                      gggCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACAt   <  1:2439154/93‑1 (MQ=255)

                                                                                      gggCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACAt   <  2:2253210/93‑1 (MQ=255)

                                                                                       ggCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACATg  <  1:3664337/93‑1 (MQ=255)

                                                                                       ggCCACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACATg  >  1:1981437/1‑93 (MQ=255)

GGCTTTAACCTGCGCTCCGCGCTCTGCTTGTGAAGTTGAACGAAAAAAATGCACTAAGTATGCACTGACTGCATAGTCACGGCAGCGGGCTACTACATCGCGTAAACTCCAGTTAATCGCATTGAGGCGAAGAGAAAGAGGAATTGCGATTTTGCTCCCGGTCTTTTCCTGAATGACATG  >  NC_000913/1199121‑1199300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`