BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,202,871	A→G	E131E (GAA→GAG)	ymfL →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,202,871	0	A	G	86.9%	579.9 / 73.9	236	E131E (GAA→GAG)	ymfL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (24/7); new base G (109/96); total (133/103)
Fisher's exact test for biased strand distribution p-value = 1.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 236 total aligned reads displayed.

CCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAATTTTTT  >  NC_000913/1202780‑1202959

  gATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCg                                                                                       >  1:4669598/1‑93 (MQ=255)

   atatTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGc                                                                                      >  2:6161326/1‑93 (MQ=255)

    tatTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCa                                                                                     <  2:156267/93‑1 (MQ=255)

      ttAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATg                                                                                   >  2:4168882/1‑93 (MQ=255)

           cAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGagaag                                                                              <  2:5913771/93‑1 (MQ=255)

            aGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGagaaga                                                                             <  2:6357645/93‑1 (MQ=255)

            aGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGagaaga                                                                             <  2:1279909/93‑1 (MQ=255)

            aGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGagaaga                                                                             <  1:1809698/93‑1 (MQ=255)

             gCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGAc                                                                            <  1:5187145/93‑1 (MQ=255)

             gCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGAc                                                                            <  2:1078460/93‑1 (MQ=255)

             gCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGAc                                                                            <  2:3561703/93‑1 (MQ=255)

              cGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACa                                                                           <  2:6572377/93‑1 (MQ=255)

              cGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACa                                                                           <  2:1048521/93‑1 (MQ=255)

              cGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACa                                                                           <  1:1421565/93‑1 (MQ=255)

               ntCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACNg                                                                          >  2:4299474/2‑93 (MQ=255)

               gTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAg                                                                          >  1:4046974/1‑93 (MQ=255)

               gTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAg                                                                          >  2:4822226/1‑93 (MQ=255)

                tCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGc                                                                         <  1:2230315/93‑1 (MQ=255)

                tCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGc                                                                         <  2:3661917/93‑1 (MQ=255)

                   gctgGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAAt                                                                      <  1:3141301/93‑1 (MQ=255)

                   gctgGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAAt                                                                      <  1:148564/93‑1 (MQ=255)

                   gctgGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAAt                                                                      >  1:6598448/1‑93 (MQ=255)

                     tgGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTa                                                                    >  1:3537511/1‑93 (MQ=255)

                     tgGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTa                                                                    <  2:2405113/93‑1 (MQ=255)

                      ggAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTaa                                                                   <  2:1629373/93‑1 (MQ=255)

                        aaGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAacg                                                                 >  1:4743170/1‑93 (MQ=255)

                        aaGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAacg                                                                 >  2:7304015/1‑93 (MQ=255)

                        aaGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAacg                                                                 >  2:409316/1‑93 (MQ=255)

                          gTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAacgac                                                               >  2:4560579/1‑93 (MQ=255)

                           tCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAacgacg                                                              >  2:3284922/1‑93 (MQ=255)

                           tCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAacgacg                                                              <  1:7493114/93‑1 (MQ=255)

                            cATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAAcgacga                                                             <  2:2470807/93‑1 (MQ=255)

                             aTTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAg                                                            >  1:486433/1‑93 (MQ=255)

                              ttGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGc                                                           >  2:1827059/1‑93 (MQ=255)

                              ttGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGc                                                           <  2:4504592/93‑1 (MQ=255)

                               tGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCt                                                          >  2:5399739/1‑93 (MQ=255)

                               tGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCt                                                          >  2:3679737/1‑93 (MQ=255)

                               tGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAACACAGCAATTAACGACGAGCt                                                          >  1:3549768/1‑93 (MQ=255)

                                gAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTg                                                         >  1:7418142/1‑93 (MQ=255)

                                gAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTg                                                         >  1:2829040/1‑93 (MQ=255)

                                gAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGccg                                                         >  2:576422/1‑91 (MQ=255)

                                 aaCAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGt                                                        <  2:3061695/93‑1 (MQ=255)

                                  aCAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTa                                                       >  1:5999216/1‑93 (MQ=255)

                                  aCAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTa                                                       <  1:4514465/93‑1 (MQ=255)

                                   cAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGCCGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAt                                                      >  2:6742695/1‑93 (MQ=255)

                                   cAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAt                                                      <  2:5976259/93‑1 (MQ=255)

                                    aGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtc                                                     >  1:6373683/1‑93 (MQ=255)

                                    aGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtc                                                     <  2:6526295/93‑1 (MQ=255)

                                     gATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtct                                                    >  1:2162462/1‑93 (MQ=255)

                                       tCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtctct                                                  <  1:3979362/93‑1 (MQ=255)

                                       tCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtctct                                                  >  1:1103076/1‑93 (MQ=255)

                                       tCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTAtctct                                                  >  1:5296333/1‑93 (MQ=255)

                                         ggCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCa                                                >  1:4366243/1‑93 (MQ=255)

                                         ggCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCa                                                >  1:2410289/1‑93 (MQ=255)

                                         ggCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCa                                                <  2:5312162/93‑1 (MQ=255)

                                          gCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCaa                                               >  2:3633040/1‑93 (MQ=255)

                                          gCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCaa                                               >  1:972052/1‑93 (MQ=255)

                                            aGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAAtt                                             <  1:7063230/93‑1 (MQ=255)

                                            aGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAAtt                                             <  1:209144/93‑1 (MQ=255)

                                             gTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAAttt                                            >  1:1706502/1‑93 (MQ=255)

                                             gTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAAttt                                            <  1:5559108/93‑1 (MQ=255)

                                              ttATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTc                                           >  2:3367434/1‑93 (MQ=255)

                                              ttATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTc                                           >  2:920421/1‑93 (MQ=255)

                                              ttATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTc                                           <  1:5593549/93‑1 (MQ=255)

                                              ttATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTc                                           >  1:1909855/1‑93 (MQ=255)

                                              ttATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTc                                           >  2:7055810/1‑93 (MQ=255)

                                                aTTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGa                                         <  1:2117108/93‑1 (MQ=255)

                                                 ttCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGaa                                        >  2:6631109/1‑93 (MQ=255)

                                                 ttCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGaa                                        >  1:4104832/1‑93 (MQ=255)

                                                 ttCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGaa                                        >  2:6228647/1‑93 (MQ=255)

                                                  tCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAg                                       <  2:5456040/93‑1 (MQ=255)

                                                    aaaaCAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCt                                     <  2:1701008/93‑1 (MQ=255)

                                                      aaCAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGc                                   >  2:1180062/1‑93 (MQ=255)

                                                       aCAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCa                                  <  1:5013334/93‑1 (MQ=255)

                                                         aGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAgg                                >  1:5128509/1‑93 (MQ=255)

                                                         aGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAgg                                <  1:2724774/93‑1 (MQ=255)

                                                         aGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAgg                                >  2:773543/1‑93 (MQ=255)

                                                          gATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGa                               >  2:2709578/1‑93 (MQ=255)

                                                           aTTCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAg                              >  1:1281396/1‑93 (MQ=255)

                                                             tCGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCa                            <  2:258263/93‑1 (MQ=255)

                                                              cGTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCAt                           <  2:2007601/93‑1 (MQ=255)

                                                               gTTCAGCAATCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATg                          >  1:782247/1‑93 (MQ=255)

                                                                      aaTCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACt                   >  1:1016174/1‑93 (MQ=255)

                                                                       aTCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACtt                  >  1:5522294/1‑93 (MQ=255)

                                                                       aTCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACtt                  >  1:6695682/1‑93 (MQ=255)

                                                                        tCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTg                 >  2:4970239/1‑93 (MQ=255)

                                                                        tCGAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTg                 >  1:6155501/1‑93 (MQ=255)

                                                                        tCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTg                 >  1:1121549/1‑93 (MQ=255)

                                                                          gAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTc               <  1:4619770/93‑1 (MQ=255)

                                                                          gAAGACGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTc               >  2:4909490/1‑93 (MQ=255)

                                                                           aaGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCt              <  1:3801870/93‑1 (MQ=255)

                                                                            aGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTa             >  2:3886741/1‑93 (MQ=255)

                                                                              aCGGTGTAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACa           >  2:1734774/1‑93 (MQ=255)

                                                                                 gtgtAGTGGAGCCGCATGAGAAGACAGAAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACaaaa        >  2:5174312/1‑93 (MQ=255)

                                                                                   gtAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAtt      >  2:4781660/1‑93 (MQ=255)

                                                                                    tAGTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAttt     >  1:5373493/1‑93 (MQ=255)

                                                                                    tAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAttt     >  2:2281158/1‑93 (MQ=255)

                                                                                      gTGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAttttt   <  2:3222573/93‑1 (MQ=255)

                                                                                       tGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAtttttt  >  2:46146/1‑93 (MQ=255)

                                                                                       tGGAGCCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAAtttttt  <  1:719584/93‑1 (MQ=255)

CCGATATTAACCAGCGTCTGCTGGAAGTCATTGAACAGATCGGCAGTTATTCAAAACAGATTCGTTCAGCAATCGAAGACGGTGTAGTGGAACCGCATGAGAAGACAGCAATTAACGACGAGCTGTATCTCTCAATTTCGAAGCTGCAGGAGCATGCAGCACTTGTCTACAAAATTTTTT  >  NC_000913/1202780‑1202959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`