BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,210,134	A→G	Y189C (TAT→TGT)	mcrA →	McrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,210,134	0	A	G	83.5%	475.9 / 90.8	212	Y189C (TAT→TGT)	mcrA	McrA
Reads supporting (aligned to +/- strand): ref base A (19/16); new base G (86/91); total (105/107)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 215 total aligned reads displayed.

ATATGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTAAAT  >  NC_000913/1210044‑1210225

atatGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTtgtg                                                                                           >  2:396212/1‑93 (MQ=255)

atatGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATg                                                                                           >  2:7076825/1‑93 (MQ=255)

 tatGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTgtgt                                                                                          <  1:4934520/93‑1 (MQ=255)

  atGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTc                                                                                         >  1:7168377/1‑93 (MQ=255)

  atGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTc                                                                                         >  1:3646966/1‑93 (MQ=255)

  atGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTc                                                                                         >  2:3726324/1‑93 (MQ=255)

   tGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTcc                                                                                        <  1:4609638/93‑1 (MQ=255)

   tGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTATcc                                                                                        >  1:5925176/1‑93 (MQ=255)

   tGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACACGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTcc                                                                                        >  1:7516936/1‑93 (MQ=255)

     aGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCga                                                                                      <  1:2954949/93‑1 (MQ=255)

     aGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCga                                                                                      <  2:3891142/93‑1 (MQ=255)

     aGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCga                                                                                      <  2:431289/93‑1 (MQ=255)

      gggTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCgag                                                                                     >  2:6151721/1‑93 (MQ=255)

       ggTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCgaga                                                                                    >  1:2691338/1‑93 (MQ=255)

       ggTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCgaga                                                                                    <  1:2292196/93‑1 (MQ=255)

       ggTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCgaga                                                                                    <  2:5095657/93‑1 (MQ=255)

         ttAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGAcc                                                                                  >  2:754474/1‑93 (MQ=255)

           aGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCg                                                                                >  1:1555872/1‑93 (MQ=255)

            gTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGa                                                                               >  1:5894324/1‑93 (MQ=255)

              aaaTTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATg                                                                             <  1:1449094/93‑1 (MQ=255)

              aaaTTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATg                                                                             <  2:1984788/93‑1 (MQ=255)

              aaaTTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATg                                                                             >  1:2748776/1‑93 (MQ=255)

               aattaattAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATgg                                                                            <  2:570401/93‑1 (MQ=255)

                attaattaAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGt                                                                           >  1:7318132/1‑93 (MQ=255)

                 ttaattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTa                                                                          >  2:6330941/1‑93 (MQ=255)

                 ttaattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTa                                                                          >  1:4002269/1‑93 (MQ=255)

                  taattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTaa                                                                         <  2:2193549/93‑1 (MQ=255)

                  taattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTaa                                                                         >  2:1413014/1‑93 (MQ=255)

                   aattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTaaa                                                                        >  1:4157576/1‑93 (MQ=255)

                   aattaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGGAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTaaa                                                                        <  1:4420886/93‑1 (MQ=255)

                    attaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTaaaa                                                                       >  1:2304492/1‑93 (MQ=255)

                     ttaaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAg                                                                      >  2:5551988/1‑93 (MQ=255)

                       aaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCt                                                                    <  1:500322/93‑1 (MQ=255)

                       aaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCt                                                                    <  2:1943897/93‑1 (MQ=255)

                       aaGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCt                                                                    <  1:6081839/93‑1 (MQ=255)

                        aGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCtt                                                                   <  2:4615217/93‑1 (MQ=255)

                         gAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTg                                                                  <  2:6663947/93‑1 (MQ=255)

                          aaaaCGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTgg                                                                 <  2:2160251/93‑1 (MQ=255)

                            aaCGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGAt                                                               <  1:643592/93‑1 (MQ=255)

                             aCGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGAtt                                                              <  2:4149883/93‑1 (MQ=255)

                             aCGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGAtt                                                              >  2:2550538/1‑93 (MQ=255)

                              cGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGAttt                                                             >  1:2085821/1‑93 (MQ=255)

                                ttGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTa                                                           <  2:1725447/93‑1 (MQ=255)

                                 tGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTAc                                                          <  2:540682/93‑1 (MQ=255)

                                 tGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTAc                                                          <  2:719004/93‑1 (MQ=255)

                                  gAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACa                                                         <  2:4818415/93‑1 (MQ=255)

                                    gTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGc                                                       <  1:324221/93‑1 (MQ=255)

                                    gTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGc                                                       <  1:3093453/93‑1 (MQ=255)

                                       aaCCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCaaa                                                    <  2:6489310/93‑1 (MQ=255)

                                       aaCCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCaaa                                                    >  1:3550051/1‑93 (MQ=255)

                                        aCCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAg                                                   >  2:3017916/1‑93 (MQ=255)

                                           cGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaa                                                >  2:6040621/1‑93 (MQ=255)

                                           cGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaa                                                >  1:6665491/1‑93 (MQ=255)

                                            gAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaaa                                               <  2:3516541/93‑1 (MQ=255)

                                            gAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaaa                                               <  2:20075/93‑1 (MQ=255)

                                            gAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaaa                                               >  2:7424842/1‑93 (MQ=255)

                                            gAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTaaa                                               <  1:1672739/93‑1 (MQ=255)

                                             aGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAg                                              <  1:1887407/93‑1 (MQ=255)

                                             aGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAg                                              >  2:1213263/1‑93 (MQ=255)

                                              gggCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAgg                                             >  2:1613578/1‑93 (MQ=255)

                                              gggCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAgg                                             >  2:2883585/1‑93 (MQ=255)

                                                gCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGta                                           <  1:3278212/93‑1 (MQ=255)

                                                gCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGta                                           <  2:3555686/93‑1 (MQ=255)

                                                  tCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGtata                                         <  1:4069155/93‑1 (MQ=255)

                                                    cAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATAtg                                       <  1:1035386/93‑1 (MQ=255)

                                                     aGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATAtgt                                      <  2:4167903/93‑1 (MQ=255)

                                                     aGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATAtgt                                      >  2:2259401/1‑93 (MQ=255)

                                                     aGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATAtgt                                      >  1:6241186/1‑93 (MQ=255)

                                                       gAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGa                                    <  1:3879147/93‑1 (MQ=255)

                                                        aaaCCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGaa                                   <  2:5143219/93‑1 (MQ=255)

                                                         aaCCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGaaa                                  >  1:5040197/1‑93 (MQ=255)

                                                          aCCAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAGGGTATATGTGaaaa                                 <  1:137516/93‑1 (MQ=255)

                                                            cAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACt                               <  2:4708337/93‑1 (MQ=255)

                                                            cAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACt                               <  1:1242384/93‑1 (MQ=255)

                                                            cAGTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACt                               >  1:7456133/1‑93 (MQ=255)

                                                              gTTGAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACtgt                             <  2:2434437/93‑1 (MQ=255)

                                                                 gAGGTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGt                          <  1:4282400/93‑1 (MQ=255)

                                                                  aGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTa                         <  1:5828446/93‑1 (MQ=255)

                                                                    gTAGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTaaa                       >  1:6941510/1‑93 (MQ=255)

                                                                     tAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTaaaa                      >  1:4238863/1‑93 (MQ=255)

                                                                      aGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTaaaaa                     >  2:3359667/1‑93 (MQ=255)

                                                                      aGAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTaaaaa                     <  2:2735456/93‑1 (MQ=255)

                                                                       gAAAGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAAt                    <  1:5158201/93‑1 (MQ=255)

                                                                       gAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAAt                    >  2:3131106/1‑93 (MQ=255)

                                                                        aaaGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAAta                   >  2:7374335/1‑92 (MQ=255)

                                                                         aaGACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGc                  >  1:450228/1‑93 (MQ=255)

                                                                           gACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTc                >  1:469162/1‑93 (MQ=255)

                                                                           gACTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTc                <  1:6806942/93‑1 (MQ=255)

                                                                            aCTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTcc               <  2:5256756/93‑1 (MQ=255)

                                                                             cTACAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCg              >  2:6735629/1‑93 (MQ=255)

                                                                               aCAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGtt            <  2:1519245/93‑1 (MQ=255)

                                                                                cAAAAAGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGttt           <  1:5696283/93‑1 (MQ=255)

                                                                                cAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGttt           >  2:7059419/1‑93 (MQ=255)

                                                                                  aaaaGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTttta         >  2:5475263/1‑93 (MQ=255)

                                                                                   aaaGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTtttat        >  2:459438/1‑93 (MQ=255)

                                                                                    aaGTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTtttatt       <  1:2638362/93‑1 (MQ=255)

                                                                                      gTTTGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTtttattta     >  2:4447617/1‑93 (MQ=255)

                                                                                       tttGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTaa    <  1:1396408/93‑1 (MQ=255)

                                                                                       tttGTGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTaa    >  2:1301973/1‑93 (MQ=255)

                                                                                         tATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTAAat  >  2:5899880/1‑93 (MQ=255)

ATATGAGGGTTAGTAAATTAATTAAGAAAACGTTGAGTCAACCCGAGGGCTCCAGGAAACCAGTTGAGGTAGAAAGACTACAAAAAGTTTATGTCCGAGACCCGATGGTAAAAGCTTGGATTTTACAGCAAAGTAAAGGTATATGTGAAAACTGTGGTAAAAATGCTCCGTTTTATTTAAAT  >  NC_000913/1210044‑1210225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`