BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,957	A→G	E310E (GAA→GAG)	fruR →	FruR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,957	0	A	G	88.0%	593.4 / 65.6	241	E310E (GAA→GAG)	fruR	FruR
Reads supporting (aligned to +/- strand): ref base A (16/13); new base G (111/101); total (127/114)
Fisher's exact test for biased strand distribution p-value = 8.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 243 total aligned reads displayed.

GACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATA  >  NC_000913/88865‑89048

  cttcttACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGcc                                                                                           >  2:7200249/1‑93 (MQ=255)

   ttcttACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCcg                                                                                          >  1:1143212/1‑93 (MQ=255)

        aCAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTaa                                                                                     <  1:3416229/93‑1 (MQ=255)

        aCAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTaa                                                                                     >  2:1776368/1‑93 (MQ=255)

        aCAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTaa                                                                                     <  1:6933543/93‑1 (MQ=255)

        aCAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTaa                                                                                     <  2:25866/93‑1 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGc                                                                                   <  1:1621534/93‑1 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGc                                                                                   >  1:4831898/1‑93 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGc                                                                                   >  2:1533760/1‑93 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGc                                                                                   >  2:4423039/1‑93 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGc                                                                                   >  1:6122820/1‑93 (MQ=255)

          aGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGc                                                                                   >  2:6437332/1‑93 (MQ=255)

           gtgtCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGcc                                                                                  >  1:6603180/1‑93 (MQ=255)

             gtCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGGGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaa                                                                                <  1:1287546/93‑1 (MQ=255)

             gtCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaa                                                                                <  2:7355713/93‑1 (MQ=255)

              tCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaaa                                                                               >  1:2449091/1‑93 (MQ=255)

              tCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaaa                                                                               >  2:5605602/1‑93 (MQ=255)

               ccGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaaaa                                                                              <  2:6173698/93‑1 (MQ=255)

               ccGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCaaaa                                                                              <  2:5945655/93‑1 (MQ=255)

                 ggTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAAcc                                                                            <  1:6735504/93‑1 (MQ=255)

                  gTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCt                                                                           <  1:5028445/93‑1 (MQ=255)

                   tGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTg                                                                          <  1:3792400/93‑1 (MQ=255)

                   tGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTg                                                                          >  2:5222059/1‑93 (MQ=255)

                    gCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTgg                                                                         <  1:2067500/93‑1 (MQ=255)

                      tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGtt                                                                       <  2:2827175/93‑1 (MQ=255)

                      tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGtt                                                                       <  2:2237882/93‑1 (MQ=255)

                      tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGtt                                                                       >  2:3587357/1‑93 (MQ=255)

                      tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGtt                                                                       >  1:1398732/1‑93 (MQ=255)

                        gCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTa                                                                     <  1:3559004/93‑1 (MQ=255)

                         cAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTaa                                                                    <  2:3976306/93‑1 (MQ=255)

                          aGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAAc                                                                   <  2:6202354/93‑1 (MQ=255)

                           gTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAAcg                                                                  >  2:841995/1‑93 (MQ=255)

                                  aaCGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTa                                                           >  1:105670/1‑93 (MQ=255)

                                  aaCGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTa                                                           <  1:527722/93‑1 (MQ=255)

                                   aCGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTaa                                                          >  1:5290295/1‑93 (MQ=255)

                                   aCGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTaa                                                          <  1:6809086/93‑1 (MQ=255)

                                     gTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAAc                                                        >  1:1764010/1‑93 (MQ=255)

                                     gTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAAc                                                        >  1:7062879/1‑93 (MQ=255)

                                     gTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAAc                                                        <  1:327865/93‑1 (MQ=255)

                                      tCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACg                                                       >  2:5814928/1‑93 (MQ=255)

                                      tCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACg                                                       >  2:6561276/1‑93 (MQ=255)

                                      cacccGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACg                                                       <  1:3707036/90‑1 (MQ=255)

                                        aCCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTa                                                     <  2:6037086/93‑1 (MQ=255)

                                         ccGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTaa                                                    <  2:3494896/93‑1 (MQ=255)

                                         ccGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTaa                                                    >  2:7440189/1‑93 (MQ=255)

                                          cgcgATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAt                                                   <  2:546541/93‑1 (MQ=255)

                                           gcgATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtc                                                  <  1:3063963/93‑1 (MQ=255)

                                            cgATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtct                                                 <  2:6840143/93‑1 (MQ=255)

                                             gATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtctc                                                <  1:6127814/93‑1 (MQ=255)

                                             gATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtctc                                                >  1:7174573/1‑93 (MQ=255)

                                             gATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtctc                                                <  1:3516870/93‑1 (MQ=255)

                                             gATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtctc                                                >  1:3340348/1‑93 (MQ=255)

                                             gATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAtctc                                                >  2:1301362/1‑93 (MQ=255)

                                              aTGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATctct                                               >  2:3010024/1‑93 (MQ=255)

                                              aTGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATctct                                               >  1:2898819/1‑93 (MQ=255)

                                              aTGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATctct                                               >  2:5300712/1‑93 (MQ=255)

                                               tGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTa                                              <  2:4120819/93‑1 (MQ=255)

                                               tGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTa                                              <  1:7200434/93‑1 (MQ=255)

                                               tGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTa                                              <  2:5114370/93‑1 (MQ=255)

                                                gTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTAt                                             <  2:569493/93‑1 (MQ=255)

                                                gTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTAt                                             >  1:5439830/1‑93 (MQ=255)

                                                 tCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATc                                            <  2:1480285/93‑1 (MQ=255)

                                                 tCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATc                                            <  1:2139797/93‑1 (MQ=255)

                                                  cGCAGAGCGTGTGCGGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATcg                                           <  1:6311761/93‑1 (MQ=255)

                                                   gCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATcgc                                          <  2:5332255/93‑1 (MQ=255)

                                                   gCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATcgc                                          >  1:4539214/1‑93 (MQ=255)

                                                      gagCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATcgccgc                                       >  1:269530/1‑93 (MQ=255)

                                                          gtgtGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCgcggcg                                   >  2:1124483/1‑93 (MQ=255)

                                                          gtgtGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCgcggcg                                   >  1:2404708/1‑93 (MQ=255)

                                                          gtgtGCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCgcggcg                                   <  2:3318684/93‑1 (MQ=255)

                                                             tgCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGc                                >  1:1902028/1‑93 (MQ=255)

                                                              gCTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCt                               <  1:4665690/93‑1 (MQ=255)

                                                               cTGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTc                              <  1:886675/93‑1 (MQ=255)

                                                                tGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCa                             >  1:3163042/1‑93 (MQ=255)

                                                                tGGAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCa                             >  1:6307494/1‑93 (MQ=255)

                                                                 ggAGATTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAg                            >  1:4263404/1‑93 (MQ=255)

                                                                 ggAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAg                            >  2:6482946/1‑93 (MQ=255)

                                                                  gagaTTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGc                           >  1:4407545/1‑93 (MQ=255)

                                                                  gagaTTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGc                           <  1:6410124/93‑1 (MQ=255)

                                                                  gagaTTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGc                           >  2:2104308/1‑93 (MQ=255)

                                                                    gaTTGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCg                         <  2:4983292/93‑1 (MQ=255)

                                                                      ttGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTa                       <  2:6324091/93‑1 (MQ=255)

                                                                       tGTCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAg                      <  2:1104861/93‑1 (MQ=255)

                                                                        gTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGc                     >  1:5712151/1‑93 (MQ=255)

                                                                         tCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCt                    >  2:203800/1‑93 (MQ=255)

                                                                         tCCTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCt                    >  1:4790532/1‑93 (MQ=255)

                                                                           cTGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTaa                  >  1:990408/1‑93 (MQ=255)

                                                                           cTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTaa                  >  2:1612350/1‑93 (MQ=255)

                                                                           cTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTaa                  <  1:5975844/93‑1 (MQ=255)

                                                                            tGGCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAg                 <  1:4152557/93‑1 (MQ=255)

                                                                             ggCAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGc                <  2:1888329/93‑1 (MQ=255)

                                                                               cAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCcg              <  1:333619/93‑1 (MQ=255)

                                                                               cAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCcg              <  2:4374531/93‑1 (MQ=255)

                                                                               cAAGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCcg              >  1:5176466/1‑93 (MQ=255)

                                                                                aaGCCTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCcgc             <  2:2918008/93‑1 (MQ=255)

                                                                                   ccTGGACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGaa          <  1:1775047/93‑1 (MQ=255)

                                                                                       gACGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACaaa      >  1:1011419/1‑93 (MQ=255)

                                                                                        aCGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACaaaa     <  2:2449637/93‑1 (MQ=255)

                                                                                         cGAGCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACaaaaa    <  1:1122747/93‑1 (MQ=255)

                                                                                           aaCCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATa  >  2:47028/1‑93 (MQ=255)

GACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATA  >  NC_000913/88865‑89048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`