Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,238,083 A→G A430A (GCA→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,238,0830AG84.4% 517.0 / 90.3 230A430A (GCA→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (20/16);  new base G (85/109);  total (105/125)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 241 total aligned reads displayed.

CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCC  >  NC_000913/1237994‑1238173
                                                                                         |                                                                                          
cGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcac                                                                                         <  2:5110623/93‑1 (MQ=255)
cGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcacac                                                                                         <  1:4700244/93‑1 (MQ=255)
 gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                        >  2:3455501/1‑93 (MQ=255)
 gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                        >  1:6052423/1‑93 (MQ=255)
 gCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGcgcaca                                                                                        <  2:5688212/93‑1 (MQ=255)
   aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                      <  2:3330844/93‑1 (MQ=255)
   aCGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGc                                                                                      <  2:1844658/93‑1 (MQ=255)
     gCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTa                                                                                    <  1:2360299/93‑1 (MQ=255)
      ccAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTaa                                                                                   >  2:3216975/1‑93 (MQ=255)
        aGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAgg                                                                                 <  1:3176941/93‑1 (MQ=255)
         gCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGa                                                                                >  1:6434696/1‑93 (MQ=255)
         gCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGa                                                                                <  2:7413289/93‑1 (MQ=255)
         gCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGa                                                                                >  1:1717433/1‑93 (MQ=255)
           gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGaaa                                                                              <  2:637213/93‑1 (MQ=255)
           gATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGaaa                                                                              >  1:4783670/1‑93 (MQ=255)
             tCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACACGGCGCACACAGCTAAGGAAACg                                                                            >  1:2248743/1‑93 (MQ=255)
             tCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCACCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACg                                                                            >  1:7245180/1‑93 (MQ=255)
              cccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                           <  2:1332836/93‑1 (MQ=255)
              cccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACga                                                                           >  1:3329103/1‑93 (MQ=255)
               ccATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACgag                                                                          <  1:7294025/93‑1 (MQ=255)
                cATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACgaga                                                                         >  1:3260080/1‑93 (MQ=255)
                 atatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGAt                                                                        <  2:2706739/93‑1 (MQ=255)
                 atatGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGAt                                                                        <  1:5917759/93‑1 (MQ=255)
                   atGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGa                                                                      <  2:2869923/93‑1 (MQ=255)
                   atGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGa                                                                      <  2:4888451/93‑1 (MQ=255)
                    tGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAc                                                                     <  1:5616874/93‑1 (MQ=255)
                     gAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGAcc                                                                    <  2:3628897/93‑1 (MQ=255)
                       ggATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCg                                                                  <  2:6270528/93‑1 (MQ=255)
                         aTCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTc                                                                >  1:4082233/1‑93 (MQ=255)
                         aTCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTc                                                                <  1:4878926/93‑1 (MQ=255)
                          tCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTcc                                                               >  1:3864671/1‑93 (MQ=255)
                            tAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGa                                                             <  2:6945511/93‑1 (MQ=255)
                               gCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATAc                                                          <  1:2983213/93‑1 (MQ=255)
                                cGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACa                                                         <  1:1462782/93‑1 (MQ=255)
                                 gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAg                                                        >  2:2689146/1‑93 (MQ=255)
                                 gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCATCCGATACAg                                                        >  2:608213/1‑93 (MQ=255)
                                 gTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAg                                                        >  2:3153881/1‑93 (MQ=255)
                                   aGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGc                                                      <  1:6750599/93‑1 (MQ=255)
                                    gcgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGcc                                                     >  2:1216146/1‑93 (MQ=255)
                                     cgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCa                                                    <  1:5185336/93‑1 (MQ=255)
                                     cgcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCa                                                    >  2:3908666/1‑93 (MQ=255)
                                      gcgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAg                                                   <  2:6756165/93‑1 (MQ=255)
                                       cgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGc                                                  <  2:4313381/93‑1 (MQ=255)
                                       cgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGc                                                  <  2:1523074/93‑1 (MQ=255)
                                       cgcTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGc                                                  <  2:7338970/93‑1 (MQ=255)
                                         cTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCt                                                >  1:1522177/1‑93 (MQ=255)
                                          tACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTc                                               >  1:4013888/1‑93 (MQ=255)
                                           aCAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCg                                              <  1:4432643/93‑1 (MQ=255)
                                             aGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGAt                                            <  2:1305889/93‑1 (MQ=255)
                                              gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                           >  1:6333203/1‑93 (MQ=255)
                                              gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                           <  2:5997946/93‑1 (MQ=255)
                                              gCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATc                                           <  2:5145943/93‑1 (MQ=255)
                                              gCCGGGGATTCACGCCTTCACGCCCGGCCAATTTACATGGCGCGCACAGCTAAGGAACCGAGTTGACCCGTCCAAAGCAGGCCAGCCTCGATc                                           <  1:20144/93‑1 (MQ=255)
                                                 gTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGc                                        >  1:4194389/1‑93 (MQ=255)
                                                  tGGATTTACGCCATCACGTCCGGGCCATTTGCATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCa                                       <  2:7437340/93‑1 (MQ=255)
                                                   ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                      >  2:5358233/1‑93 (MQ=255)
                                                   ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                      >  2:1493421/1‑93 (MQ=255)
                                                   ggATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAg                                      <  1:11604/93‑1 (MQ=255)
                                                    gATTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAgg                                     <  2:5499358/93‑1 (MQ=255)
                                                     aTTTACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGc                                    <  2:7263787/93‑1 (MQ=255)
                                                      tttACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCa                                   <  1:506197/93‑1 (MQ=255)
                                                       ttACGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCAt                                  >  1:2506244/1‑93 (MQ=255)
                                                          cGCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaa                               <  2:2779464/93‑1 (MQ=255)
                                                           gCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaa                              <  2:5420453/93‑1 (MQ=255)
                                                           gCCATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaa                              >  2:6137197/1‑93 (MQ=255)
                                                            ccATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTaaaa                             >  1:7075513/1‑93 (MQ=255)
                                                             cATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAc                            >  1:1094104/1‑93 (MQ=255)
                                                             cATCACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAAc                            <  1:7024033/93‑1 (MQ=255)
                                                                cACGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGa                         <  1:7401499/93‑1 (MQ=255)
                                                                 aCGTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGaa                        <  2:435272/93‑1 (MQ=255)
                                                                   gTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATc                      >  1:176140/1‑93 (MQ=255)
                                                                   gTCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATc                      <  1:4815206/93‑1 (MQ=255)
                                                                    tCCGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCt                     >  1:6597639/1‑93 (MQ=255)
                                                                     ccGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTg                    <  1:453556/93‑1 (MQ=255)
                                                                     ccGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTg                    <  2:5837562/93‑1 (MQ=255)
                                                                      cGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGt                   >  2:6869369/1‑93 (MQ=255)
                                                                      cGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGt                   <  1:5734110/93‑1 (MQ=255)
                                                                      cGGGCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGt                   <  2:225522/93‑1 (MQ=255)
                                                                       gggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTc                  <  2:5793097/93‑1 (MQ=255)
                                                                       gggCCATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTc                  >  2:4540021/1‑93 (MQ=255)
                                                                       gggCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTc                  >  1:1932871/1‑93 (MQ=255)
                                                                          ccATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAt               <  1:2109879/93‑1 (MQ=255)
                                                                           cATTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCAtt              >  1:5777156/1‑93 (MQ=255)
                                                                            aTTTACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTg             >  1:3283295/1‑93 (MQ=255)
                                                                             tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt            >  1:4022993/1‑93 (MQ=255)
                                                                             tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt            <  1:922530/93‑1 (MQ=255)
                                                                             tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt            <  2:3008274/93‑1 (MQ=255)
                                                                             tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt            >  2:3769923/1‑93 (MQ=255)
                                                                             tttACATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGt            >  1:926995/1‑93 (MQ=255)
                                                                                aCATGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCg         >  1:4301774/1‑93 (MQ=255)
                                                                                  aTGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGcc       <  1:4441756/93‑1 (MQ=255)
                                                                                  aTGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGcc       >  2:452514/1‑93 (MQ=255)
                                                                                   tGGCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCa      <  2:5551567/93‑1 (MQ=255)
                                                                                   tGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCa      >  1:1340878/1‑93 (MQ=255)
                                                                                   tGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCa      <  2:2119504/93‑1 (MQ=255)
                                                                                    ggCGCGCACAGCTAAGGAAACGAGATGCCCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAg     <  1:2897724/93‑1 (MQ=255)
                                                                                    ggCGCGCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAg     <  1:3909743/93‑1 (MQ=255)
                                                                                     gcgcgcACAGCAAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAgg    <  1:6733319/93‑1 (MQ=255)
                                                                                      cgcgCACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGc   <  1:3485922/93‑1 (MQ=255)
                                                                                       gcACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGcc  >  1:440081/1‑93 (MQ=255)
                                                                                         |                                                                                          
CGCACGCCAGCGATCCCATATGAGGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGTCCGGGCCATTTACATGGCGCACACAGCTAAGGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGCC  >  NC_000913/1237994‑1238173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑