BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,381,611	A→G	I125I (ATT→ATC)	ycjW ←	putative LACI‑type transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,381,611	0	A	G	87.6%	488.3 / 52.8	194	I125I (ATT→ATC)	ycjW	putative LACI‑type transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (8/16); new base G (77/93); total (85/109)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 215 total aligned reads displayed.

TGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAAC  >  NC_000913/1381519‑1381702

 gACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCAtaataa                                                                                            <  1:2382846/93‑1 (MQ=255)

 gACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCAtaataa                                                                                            <  1:5620790/93‑1 (MQ=255)

  aCCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAGAAAATGACTCATCCGCTGGCGAACTGAGCATaatgat                                                                                           >  2:6789698/1‑93 (MQ=255)

  aCCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATaatgat                                                                                           <  1:4825860/93‑1 (MQ=255)

  aCCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATaatgat                                                                                           <  1:2956480/93‑1 (MQ=255)

   ccTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAatgatg                                                                                          >  2:6345885/1‑93 (MQ=255)

     ttCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGcc                                                                                        >  2:6584384/1‑93 (MQ=255)

     ttCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGcc                                                                                        <  2:3872508/93‑1 (MQ=255)

     ttCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGcc                                                                                        >  1:5356159/1‑93 (MQ=255)

     ttCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGcc                                                                                        >  2:2393352/1‑93 (MQ=255)

      tCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCt                                                                                       <  2:6085316/93‑1 (MQ=255)

      tCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCt                                                                                       <  1:6341106/93‑1 (MQ=255)

       cAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCtt                                                                                      <  1:1709596/93‑1 (MQ=255)

        aaCTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCttt                                                                                     <  1:6750847/93‑1 (MQ=255)

         aCTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTa                                                                                    <  1:313850/93‑1 (MQ=255)

           tttGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAAt                                                                                  >  2:1151893/1‑93 (MQ=255)

            ttGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATc                                                                                 <  2:802602/93‑1 (MQ=255)

             tGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCa                                                                                <  1:1339430/93‑1 (MQ=255)

              gCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAt                                                                               <  2:187858/93‑1 (MQ=255)

              gCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCAt                                                                               <  2:6400829/93‑1 (MQ=255)

               ccAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAtt                                                                              >  2:168186/1‑93 (MQ=255)

               ccAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAtt                                                                              <  1:122542/93‑1 (MQ=255)

               ccAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAtt                                                                              <  1:469294/93‑1 (MQ=255)

                cAATCACCACGACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAttt                                                                             <  2:2608734/93‑1 (MQ=255)

                cAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCAttt                                                                             <  2:1355286/93‑1 (MQ=255)

                  aTCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTc                                                                           >  1:7400378/1‑93 (MQ=255)

                     accaccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGc                                                                        <  2:4755446/93‑1 (MQ=255)

                     accaccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGc                                                                        <  2:3229011/93‑1 (MQ=255)

                     accaccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGc                                                                        >  1:2648167/1‑93 (MQ=255)

                       caccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCtt                                                                      >  1:1161855/1‑93 (MQ=255)

                       caccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCtt                                                                      >  2:5270009/1‑93 (MQ=255)

                        accaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTa                                                                     >  2:7371931/1‑93 (MQ=255)

                        accaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTa                                                                     >  2:4298336/1‑93 (MQ=255)

                         ccaccGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTaa                                                                    >  2:3456115/1‑93 (MQ=255)

                           accGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAAtt                                                                  >  1:7484770/1‑93 (MQ=255)

                           accGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAAtt                                                                  >  2:4351741/1‑93 (MQ=255)

                               gAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGc                                                              >  1:1639342/1‑93 (MQ=255)

                               gAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCACTTTCTGCTTAATTTTGc                                                              <  1:3108250/93‑1 (MQ=255)

                                 atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCtt                                                            <  2:752440/93‑1 (MQ=255)

                                 atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCtt                                                            <  2:5700297/93‑1 (MQ=255)

                                 atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCtt                                                            >  1:1937300/1‑93 (MQ=255)

                                 atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCtt                                                            >  1:1071253/1‑93 (MQ=255)

                                 atatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCtt                                                            <  1:6042849/93‑1 (MQ=255)

                                  tatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCttt                                                           >  2:7340597/1‑93 (MQ=255)

                                  tatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCttt                                                           >  2:730772/1‑93 (MQ=255)

                                  tatCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCttt                                                           <  2:4884797/93‑1 (MQ=255)

                                   atCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTc                                                          <  1:821817/93‑1 (MQ=255)

                                    tCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCg                                                         >  1:63676/1‑93 (MQ=255)

                                          ttATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCAtttt                                                   <  1:6556911/93‑1 (MQ=255)

                                           tATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCAttttt                                                  >  1:6001138/1‑93 (MQ=255)

                                             tCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGc                                                >  1:3058533/1‑93 (MQ=255)

                                              cGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCa                                               <  2:1023653/93‑1 (MQ=255)

                                              cGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCa                                               <  2:7490039/93‑1 (MQ=255)

                                               gAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAg                                              <  2:5994148/93‑1 (MQ=255)

                                                aGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGa                                             <  1:3690685/93‑1 (MQ=255)

                                                 gTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGAt                                            <  1:4406247/93‑1 (MQ=255)

                                                 gTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGAt                                            <  1:1805976/93‑1 (MQ=255)

                                                     ggCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGAtcttc                                        <  2:4009431/93‑1 (MQ=255)

                                                     ggCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGAtcttc                                        >  2:3540407/1‑93 (MQ=255)

                                                      gCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGAtcttct                                       <  2:2837146/93‑1 (MQ=255)

                                                        aaaaaaTGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGc                                     <  1:3780403/93‑1 (MQ=255)

                                                         aaaaaTGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGcc                                    <  2:5642355/93‑1 (MQ=255)

                                                          aaaaTGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCg                                   <  1:4792995/93‑1 (MQ=255)

                                                             aTGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGAt                                <  2:5511256/93‑1 (MQ=255)

                                                              tGACTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGAtt                               >  1:1877020/1‑93 (MQ=255)

                                                                 cTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATtgtg                            >  1:2041189/1‑93 (MQ=255)

                                                                 cTCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATtgtg                            <  2:770182/93‑1 (MQ=255)

                                                                  tCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGc                           >  2:1406851/1‑93 (MQ=255)

                                                                  tCATCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGc                           >  1:4520691/1‑93 (MQ=255)

                                                                    aTCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGa                         >  2:2060949/1‑93 (MQ=255)

                                                                     tCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGAc                        <  1:2285576/93‑1 (MQ=255)

                                                                     tCCGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGAc                        <  2:5532802/93‑1 (MQ=255)

                                                                       cGCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGt                      >  2:3637928/1‑93 (MQ=255)

                                                                        gCTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGtt                     <  1:7063133/93‑1 (MQ=255)

                                                                         cTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGttt                    <  2:5818600/93‑1 (MQ=255)

                                                                         cTGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGttt                    >  1:3450504/1‑93 (MQ=255)

                                                                          tGGCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTg                   >  2:1251476/1‑93 (MQ=255)

                                                                           ggCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGt                  >  2:2891136/1‑93 (MQ=255)

                                                                            gCGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTa                 <  2:7205633/93‑1 (MQ=255)

                                                                             cGAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTaa                >  1:5420164/1‑93 (MQ=255)

                                                                              gAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAat               <  2:5216915/93‑1 (MQ=255)

                                                                              gAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAat               >  2:37107/1‑93 (MQ=255)

                                                                              gAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAat               >  2:1733299/1‑93 (MQ=255)

                                                                              gAACTGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAat               >  2:686778/1‑93 (MQ=255)

                                                                                 cTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATc            <  2:4201162/93‑1 (MQ=255)

                                                                                  tGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCa           <  1:256223/93‑1 (MQ=255)

                                                                                  tGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCa           >  1:1369446/1‑93 (MQ=255)

                                                                                  tGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCa           >  2:3580597/1‑93 (MQ=255)

                                                                                  tGAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCa           <  1:2789497/93‑1 (MQ=255)

                                                                                   gAGCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCAc          >  1:1617619/1‑93 (MQ=255)

                                                                                   gAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCAc          <  2:277369/93‑1 (MQ=255)

                                                                                    nncATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCACATCTTCTGCCGGATTGTGCGCCGTTTGTACTATCACg         <  1:2144585/91‑1 (MQ=255)

                                                                                     gCATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGt        >  2:4673853/1‑93 (MQ=255)

                                                                                      cATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTc       <  2:4471929/93‑1 (MQ=255)

                                                                                      cATAATGATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTc       <  2:5369899/93‑1 (MQ=255)

                                                                                      cATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTc       <  1:6085629/93‑1 (MQ=255)

                                                                                        taatgaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGa     >  2:5030053/1‑93 (MQ=255)

                                                                                        taatgaTGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGa     >  1:3413796/1‑93 (MQ=255)

                                                                                         aatgatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGaa    >  1:7530765/1‑93 (MQ=255)

                                                                                          ataatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGaaa   <  1:2758197/93‑1 (MQ=255)

TGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAAC  >  NC_000913/1381519‑1381702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`