Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,383,611 A→G Q26R (CAG→CGG)  ycjF → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,383,6110AG87.7% 563.4 / 67.0 227Q26R (CAG→CGG) ycjFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base G (109/90);  total (120/107)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 241 total aligned reads displayed.

GGAGATAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGG  >  NC_000913/1383521‑1383703
                                                                                           |                                                                                             
ggAGATAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCgcggc                                                                                             <  2:519162/93‑1 (MQ=255)
ggAGATAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCgcggc                                                                                             <  1:1382692/93‑1 (MQ=255)
ggAGATAAATGGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCgcggc                                                                                             <  1:5856712/93‑1 (MQ=255)
 gagaTAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCgcggca                                                                                            >  2:7401407/1‑93 (MQ=255)
   gaTAAATTGCGATGGCCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGa                                                                                          >  2:6455840/1‑93 (MQ=255)
   gaTAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGa                                                                                          <  1:1537407/93‑1 (MQ=255)
   gaTAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGa                                                                                          >  1:3879057/1‑93 (MQ=255)
    aTAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGAc                                                                                         >  2:3469980/1‑93 (MQ=255)
     tAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGAcc                                                                                        >  1:3110576/1‑93 (MQ=255)
     tAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGAcc                                                                                        >  1:7249628/1‑93 (MQ=255)
      aaaTTGCGATGACCGAACCGGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGAcc                                                                                        >  1:6153251/1‑93 (MQ=255)
      aaaTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCt                                                                                       <  2:3734660/93‑1 (MQ=255)
      aaaTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCt                                                                                       <  2:5727108/93‑1 (MQ=255)
      aaaTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCt                                                                                       <  1:2713616/93‑1 (MQ=255)
      aaaTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCt                                                                                       <  1:430649/93‑1 (MQ=255)
       aaTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCtt                                                                                      <  1:1111674/93‑1 (MQ=255)
        aTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCttt                                                                                     <  2:1364659/93‑1 (MQ=255)
        aTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCttt                                                                                     <  1:3762224/93‑1 (MQ=255)
        aTTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCttt                                                                                     <  2:4654401/93‑1 (MQ=255)
          tGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGa                                                                                   <  1:467985/93‑1 (MQ=255)
           gCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGAc                                                                                  >  1:1032735/1‑93 (MQ=255)
               tGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGaaa                                                                              <  2:2772980/93‑1 (MQ=255)
                gACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGaaaa                                                                             <  2:6817858/93‑1 (MQ=255)
                gACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTCTGACGaaaa                                                                             >  1:4062225/1‑93 (MQ=255)
                  ccGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATc                                                                           <  2:2708743/93‑1 (MQ=255)
                  ccGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATc                                                                           <  1:125459/93‑1 (MQ=255)
                  ccGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATc                                                                           >  1:6628475/1‑93 (MQ=255)
                    gAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAg                                                                         >  2:7471777/1‑93 (MQ=255)
                    gAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAg                                                                         <  1:2622477/93‑1 (MQ=255)
                    gAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAg                                                                         <  1:7491590/93‑1 (MQ=255)
                    gAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAg                                                                         <  1:2080713/93‑1 (MQ=255)
                    gAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAg                                                                         <  1:4614706/93‑1 (MQ=255)
                      aCC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGgc                                                                       <  2:292167/93‑1 (MQ=255)
                      aCC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGgc                                                                       <  1:5814650/93‑1 (MQ=255)
                       cc‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGgcg                                                                      >  2:580131/1‑93 (MQ=255)
                       cc‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGgcg                                                                      >  2:200555/1‑93 (MQ=255)
                       cc‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGgcg                                                                      >  2:3039460/1‑93 (MQ=255)
                          gTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCa                                                                    <  1:5071256/93‑1 (MQ=255)
                          gTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCa                                                                    >  2:2543690/1‑93 (MQ=255)
                           ttAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCaa                                                                   >  2:4079310/1‑93 (MQ=255)
                            tAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCaaa                                                                  >  1:4163029/1‑93 (MQ=255)
                                 ccACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAAtttt                                                             >  1:6319070/1‑93 (MQ=255)
                                 ccACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGATCTTTGACGAAAATCAGGCGCAAAAtttt                                                             <  2:1350857/93‑1 (MQ=255)
                                   aCGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGc                                                           <  1:3017078/93‑1 (MQ=255)
                                   aCGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGc                                                           >  1:1923150/1‑93 (MQ=255)
                                   aCGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGc                                                           >  1:5611813/1‑93 (MQ=255)
                                    cGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGcc                                                          <  2:146750/93‑1 (MQ=255)
                                     gTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGGGCAAAATTTGGccc                                                         >  1:5015668/1‑93 (MQ=255)
                                      tATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGcccc                                                        <  2:5129432/93‑1 (MQ=255)
                                       aTTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCg                                                       >  1:325934/1‑93 (MQ=255)
                                       aTTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCg                                                       <  1:3471608/93‑1 (MQ=255)
                                          gATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGcc                                                    >  1:1175276/1‑93 (MQ=255)
                                            tttCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCAc                                                  <  1:2472124/93‑1 (MQ=255)
                                            tttCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACAAAAATCAGGCGCAAAATTTTGCCCCGGCCAc                                                  >  1:5708188/1‑93 (MQ=255)
                                               cGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGcg                                               <  1:4867265/93‑2 (MQ=255)
                                               cGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCt                                               <  1:6926584/93‑1 (MQ=255)
                                                 aCGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTcg                                             >  1:1512720/1‑93 (MQ=255)
                                                 aCGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTcg                                             >  2:3585474/1‑93 (MQ=255)
                                                     tCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTcgacg‑a                                        >  2:258917/1‑93 (MQ=255)
                                                      ccTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑aa                                       <  1:2300851/93‑1 (MQ=255)
                                                      ccTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑aa                                       <  2:6654803/93‑1 (MQ=255)
                                                       ctctGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAg                                      >  2:5605790/1‑93 (MQ=255)
                                                        tctGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgc                                     <  2:4911912/93‑1 (MQ=255)
                                                        tctGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgc                                     >  1:6046534/1‑93 (MQ=255)
                                                        tctGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgc                                     >  1:3606540/1‑93 (MQ=255)
                                                          tGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgcgc                                   >  1:299954/1‑93 (MQ=255)
                                                          tGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgcgc                                   >  2:2281740/1‑93 (MQ=255)
                                                          tGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgcgc                                   >  1:1745900/1‑93 (MQ=255)
                                                          tGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgcgc                                   >  2:5068113/1‑93 (MQ=255)
                                                          tGGAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAgcgc                                   >  1:7376527/1‑93 (MQ=255)
                                                            gAGGTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAg                                 <  2:7280595/93‑1 (MQ=255)
                                                            gAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAg                                 <  2:1867719/93‑1 (MQ=255)
                                                              ggTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGga                               <  2:3229124/93‑1 (MQ=255)
                                                               gTCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGgaa                              <  2:5968733/93‑1 (MQ=255)
                                                               gTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGgaa                              <  1:3934946/93‑1 (MQ=255)
                                                                tCGATCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGgaag                             >  2:6267862/1‑93 (MQ=255)
                                                                   aTCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGaagaag                          >  1:5301882/1‑93 (MQ=255)
                                                                   aTCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGaagaag                          <  1:1963691/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  2:96835/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  1:1854442/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  2:4539263/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  1:2907431/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  1:6252809/93‑1 (MQ=255)
                                                                    tCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAagaaga                         <  2:5384241/93‑1 (MQ=255)
                                                                       gAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAAGCGCAGGAAGAAGAgg                       >  1:2376122/1‑93 (MQ=255)
                                                                       gAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAggg                      >  2:2570171/1‑93 (MQ=255)
                                                                       gAATCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAggg                      >  2:6130181/1‑93 (MQ=255)
                                                                        aaTCCTAAATTCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAgggg                     >  2:5855860/1‑93 (MQ=255)
                                                                           ccTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCaa                  <  2:4984479/93‑1 (MQ=255)
                                                                            cTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAg                 >  1:6998317/1‑93 (MQ=255)
                                                                             tAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGt                >  2:3713689/1‑93 (MQ=255)
                                                                                 ttCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGaa            >  2:4241758/1‑93 (MQ=255)
                                                                                 ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGaa            <  2:4405688/93‑1 (MQ=255)
                                                                                  tCAGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAg           <  1:7078752/93‑1 (MQ=255)
                                                                                    aGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCg         <  2:597968/93‑1 (MQ=255)
                                                                                    aGGGCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCg         >  1:2565270/1‑93 (MQ=255)
                                                                                    aGGGCGCGGCAGACCTTTGACGAAAACCAGGCGCAAAAGTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCg         <  2:3891520/93‑1 (MQ=255)
                                                                                     gggCGCGGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCgg        <  1:680520/93‑1 (MQ=255)
                                                                                        cgcgGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTaa     <  2:2305676/93‑1 (MQ=255)
                                                                                           agcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATgg  >  1:1582465/1‑93 (MQ=255)
                                                                                           |                                                                                             
GGAGATAAATTGCGATGACCGAACC‑GTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACG‑AAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGG  >  NC_000913/1383521‑1383703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑