BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,397,365	A→G	G62G (GGT→GGC)	fnr ←	Fnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,365	0	A	G	80.9%	619.5 / 139.4	298	G62G (GGT→GGC)	fnr	Fnr
Reads supporting (aligned to +/- strand): ref base A (22/35); new base G (109/132); total (131/167)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 309 total aligned reads displayed.

GAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGA  >  NC_000913/1397275‑1397456

 aaaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCa                                                                                          <  1:2725170/93‑1 (MQ=255)

 aaaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCa                                                                                          <  2:4221405/93‑1 (MQ=255)

  aaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAg                                                                                         >  1:5267249/1‑93 (MQ=255)

  aaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAg                                                                                         >  1:2757692/1‑93 (MQ=255)

   aCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGc                                                                                        >  2:5736465/1‑93 (MQ=255)

    ccAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGcc                                                                                       >  1:504323/1‑93 (MQ=255)

        tGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTaa                                                                                   <  1:2884219/93‑1 (MQ=255)

        tGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTaa                                                                                   >  1:5115523/1‑93 (MQ=255)

        tGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTaa                                                                                   >  1:6845544/1‑93 (MQ=255)

          aTTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAAc                                                                                 >  1:3596033/1‑93 (MQ=255)

            ttGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAg                                                                               <  1:6444126/93‑1 (MQ=255)

             tGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGc                                                                              <  2:1344947/93‑1 (MQ=255)

             tGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGc                                                                              <  1:5756538/93‑1 (MQ=255)

              gCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCg                                                                             <  1:6081549/93‑1 (MQ=255)

              gCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg                                                                             <  2:1089491/93‑1 (MQ=255)

               cTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGt                                                                            <  2:5334092/93‑1 (MQ=255)

               cTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGt                                                                            >  1:2214549/1‑93 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                           <  1:6787977/93‑1 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                           >  1:1035179/1‑93 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                           >  1:3754072/1‑93 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                           <  2:4193516/93‑1 (MQ=255)

                  gtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTg                                                                         <  1:3514507/93‑1 (MQ=255)

                  gtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTg                                                                         >  2:4560509/1‑93 (MQ=255)

                  gtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTg                                                                         >  1:3329940/1‑93 (MQ=255)

                     gCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGcc                                                                      <  2:5648607/93‑1 (MQ=255)

                      ccTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCt                                                                     <  2:2991386/93‑1 (MQ=255)

                        ttGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCttt                                                                   >  2:7217594/1‑93 (MQ=255)

                            tCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGa                                                               >  2:6390167/1‑93 (MQ=255)

                            tCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGa                                                               >  1:1726769/1‑93 (MQ=255)

                            tCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGa                                                               >  1:5976754/1‑93 (MQ=255)

                              aGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAAt                                                             <  2:2405926/93‑1 (MQ=255)

                              aGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAAt                                                             <  2:385511/93‑1 (MQ=255)

                               gTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATa                                                            <  1:906306/93‑1 (MQ=255)

                                 gATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAgg                                                          >  1:2371158/1‑93 (MQ=255)

                                   tGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGct                                                        <  2:1802162/93‑1 (MQ=255)

                                     gTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttc                                                      <  1:4388192/93‑1 (MQ=255)

                                     gTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttc                                                      <  1:6535577/93‑1 (MQ=255)

                                     gTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttc                                                      <  1:1315163/93‑1 (MQ=255)

                                      tataACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttct                                                     <  1:1853016/93‑1 (MQ=255)

                                      tataACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttct                                                     >  2:2876631/1‑93 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                   <  1:2970977/93‑1 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                   <  2:6785035/93‑1 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                   >  1:3640842/1‑93 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGATCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                   >  2:1854698/1‑93 (MQ=255)

                                         aaCTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTcc                                                  >  2:7518961/1‑93 (MQ=255)

                                            ttttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCt                                               <  2:229741/93‑1 (MQ=255)

                                            ttttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCt                                               >  1:3359763/1‑93 (MQ=255)

                                             tttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTc                                              >  1:5399327/1‑93 (MQ=255)

                                             tttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTc                                              >  2:6168871/1‑93 (MQ=255)

                                             tttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTc                                              <  1:4213642/93‑1 (MQ=255)

                                              ttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCa                                             >  2:7008691/1‑93 (MQ=255)

                                              ttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCa                                             <  1:6282972/93‑1 (MQ=255)

                                                aaTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAAt                                           >  2:1240991/1‑93 (MQ=255)

                                                 aTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATg                                          >  2:5256861/1‑93 (MQ=255)

                                                 aTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATg                                          >  1:1525917/1‑93 (MQ=255)

                                                 aTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATg                                          <  2:7086116/93‑1 (MQ=255)

                                                  tCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGa                                         >  2:5813654/1‑93 (MQ=255)

                                                  tCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGa                                         <  2:5709404/93‑1 (MQ=255)

                                                    gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGata                                       >  2:3042315/1‑93 (MQ=255)

                                                    gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGata                                       <  2:3302168/93‑1 (MQ=255)

                                                    gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGata                                       >  2:5423736/1‑93 (MQ=255)

                                                    gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGata                                       >  1:1127346/1‑93 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                     <  1:1244037/93‑1 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                     <  1:6223509/93‑1 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                     <  2:3594403/93‑1 (MQ=255)

                                                        cGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtattat                                   <  1:3205556/93‑1 (MQ=255)

                                                         ggAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATc                                  >  1:1688628/1‑93 (MQ=255)

                                                         ggAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATc                                  >  2:5300290/1‑93 (MQ=255)

                                                            gCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAg                               <  2:2127298/93‑1 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                             >  2:7337120/1‑93 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                             >  1:5649978/1‑93 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                             <  1:472003/93‑1 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                             <  1:1777666/93‑1 (MQ=255)

                                                               gATGGCATAAGGCGATTTAAGTCCACCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGTTTCTCCCGCTCAATGATATT‑TCAAGCTGa                           <  1:7168348/93‑1 (MQ=255)

                                                               gATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTg                            >  2:347134/1‑93 (MQ=255)

                                                                aTGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGa                           >  1:7091511/1‑93 (MQ=255)

                                                                  ggCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATc                         <  2:1507890/93‑1 (MQ=255)

                                                                    cATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCaa                       >  2:1644750/1‑93 (MQ=255)

                                                                    cATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCaa                       >  2:4380030/1‑93 (MQ=255)

                                                                     aTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAg                      >  1:3060040/1‑93 (MQ=255)

                                                                     aTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAg                      >  1:3929213/1‑93 (MQ=255)

                                                                     aTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAg                      >  2:4653095/1‑93 (MQ=255)

                                                                      tAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGc                     >  1:6389853/1‑93 (MQ=255)

                                                                       aaaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCt                    <  1:4523883/93‑1 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                   <  1:4868730/93‑1 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                   >  1:4070354/1‑93 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                   <  1:2015079/93‑1 (MQ=255)

                                                                          gCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCAt                 <  1:2614892/93‑1 (MQ=255)

                                                                           cGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATg                <  2:1915566/93‑1 (MQ=255)

                                                                            gATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGt               >  1:1948162/1‑93 (MQ=255)

                                                                            gATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGt               >  1:5860355/1‑93 (MQ=255)

                                                                            gATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGt               >  2:7421669/1‑93 (MQ=255)

                                                                             aTTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGtt              <  1:7480496/93‑1 (MQ=255)

                                                                                 aaGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGtt          <  2:6525577/93‑1 (MQ=255)

                                                                                     tcatcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgt      <  2:609879/93‑1 (MQ=255)

                                                                                     tcatcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgt      <  1:1415830/93‑1 (MQ=255)

                                                                                     tcatcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgt      >  1:7016453/1‑93 (MQ=255)

                                                                                      catcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtg     <  2:4934494/93‑1 (MQ=255)

                                                                                      catcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtg     <  2:3779899/93‑1 (MQ=255)

                                                                                       atcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtgt    <  2:3267704/93‑1 (MQ=255)

GAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGA  >  NC_000913/1397275‑1397456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`