BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,063	A→G	V9A (GTC→GCC)	b1436 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,063	0	A	G	83.9%	657.0 / 121.0	291	V9A (GTC→GCC)	b1436	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (27/20); new base G (118/126); total (145/146)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 292 total aligned reads displayed.

CAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTT  >  NC_000913/1506972‑1507155

cAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGAc                                                                                             >  2:204790/1‑93 (MQ=255)

  gCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCaa                                                                                           >  2:2597810/1‑93 (MQ=255)

   cTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAAc                                                                                          >  2:6617654/1‑93 (MQ=255)

     gatgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAATCAGAGCACACGTTAATAAGGCAACCg                                                                                        >  1:4833121/1‑93 (MQ=255)

      atgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGa                                                                                       <  2:4929200/93‑1 (MQ=255)

       tgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGAt                                                                                      >  1:1940123/1‑93 (MQ=255)

       tgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGAt                                                                                      >  2:2135899/1‑93 (MQ=255)

        gatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATa                                                                                     <  2:5130558/93‑1 (MQ=255)

        gatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATa                                                                                     >  1:2610571/1‑93 (MQ=255)

        gatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTACCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATa                                                                                     >  2:3860960/1‑93 (MQ=255)

         atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATaa                                                                                    >  1:6902001/1‑93 (MQ=255)

         atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATaa                                                                                    >  1:3941707/1‑93 (MQ=255)

         atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATaa                                                                                    >  1:3342190/1‑93 (MQ=255)

         atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATaa                                                                                    >  2:4657964/1‑93 (MQ=255)

          tCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAAc                                                                                   <  2:5009856/93‑1 (MQ=255)

          tCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAAc                                                                                   <  2:6245524/93‑1 (MQ=255)

          tCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAAc                                                                                   >  1:2476167/1‑93 (MQ=255)

             acaTTCTTTACCCACACAAATTGATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGcc                                                                                >  2:5994592/1‑93 (MQ=255)

              caTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCt                                                                               >  2:2659751/1‑93 (MQ=255)

               aTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCtt                                                                              >  1:5735546/1‑93 (MQ=255)

                ttCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTc                                                                             >  2:5655792/1‑93 (MQ=255)

                 tCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCg                                                                            >  2:2896902/1‑93 (MQ=255)

                  cTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGt                                                                           >  2:6025499/1‑93 (MQ=255)

                   tttACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTa                                                                          >  1:3179432/1‑93 (MQ=255)

                   tttACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTa                                                                          >  1:1751212/1‑93 (MQ=255)

                       cccACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACa                                                                      >  2:2241714/1‑93 (MQ=255)

                       cccACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACa                                                                      <  1:1125246/93‑1 (MQ=255)

                        ccACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACAt                                                                     >  1:4000524/1‑93 (MQ=255)

                        ccACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACAt                                                                     <  1:2968238/93‑1 (MQ=255)

                         cacacaAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATc                                                                    >  1:5782968/1‑93 (MQ=255)

                          acacaAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATcc                                                                   <  1:7446071/93‑1 (MQ=255)

                           cacaAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCt                                                                  <  1:534023/93‑1 (MQ=255)

                            acaAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTg                                                                 >  1:2089322/1‑93 (MQ=255)

                                aTTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCAc                                                             >  2:2492705/1‑93 (MQ=255)

                                 ttCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCAcc                                                            >  1:2890326/1‑93 (MQ=255)

                                  tCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCt                                                           <  2:2831839/93‑1 (MQ=255)

                                  tCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCt                                                           >  1:701227/1‑93 (MQ=255)

                                     tGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCtttt                                                        >  2:4076603/1‑93 (MQ=255)

                                       tCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTCCGTAAACATCCTGCCACCTTTTTc                                                      <  1:955161/93‑1 (MQ=255)

                                         cTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCtt                                                    >  2:6315214/1‑93 (MQ=255)

                                         cTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCtt                                                    <  1:660813/93‑1 (MQ=255)

                                         cTTTGCGCCTTGCTATGAGCTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGAGAACGCCTTCGTAAACATCCTGCCACCTTTTTCtt                                                    >  1:4878309/1‑93 (MQ=255)

                                          tttGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTa                                                   >  2:246718/1‑93 (MQ=255)

                                          tttGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTa                                                   <  2:4590625/93‑1 (MQ=255)

                                           ttGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTAc                                                  <  2:1508412/93‑1 (MQ=255)

                                            tgtgCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTAcc                                                 <  1:6429963/93‑1 (MQ=255)

                                             gtgCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCt                                                <  1:1226746/93‑1 (MQ=255)

                                               gCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCttt                                              <  2:1218489/93‑1 (MQ=255)

                                               gCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCttt                                              <  1:1543343/93‑1 (MQ=255)

                                               gCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCttt                                              >  1:1333821/1‑93 (MQ=255)

                                                  cTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGc                                           <  2:7422200/93‑1 (MQ=255)

                                                  cTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGc                                           <  2:6865826/93‑1 (MQ=255)

                                                  cTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGc                                           >  2:3649401/1‑93 (MQ=255)

                                                   tGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGcc                                          <  2:3289935/93‑1 (MQ=255)

                                                   tGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGcc                                          >  2:7286302/1‑93 (MQ=255)

                                                    gCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCg                                         >  2:5566853/1‑93 (MQ=255)

                                                     ccATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGc                                        >  2:2716116/1‑93 (MQ=255)

                                                       aTGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTa                                      >  1:6284073/1‑93 (MQ=255)

                                                        tGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTat                                     >  2:3420493/1‑93 (MQ=255)

                                                        tGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTat                                     >  2:7163954/1‑93 (MQ=255)

                                                        tGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTat                                     <  2:1652601/93‑1 (MQ=255)

                                                         gAGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatg                                    >  1:1454343/1‑93 (MQ=255)

                                                          aGTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatga                                   <  2:6200379/93‑1 (MQ=255)

                                                           gTTGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatgat                                  <  1:5002736/93‑1 (MQ=255)

                                                             tGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGc                                <  2:6428961/93‑1 (MQ=255)

                                                             tGTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGc                                >  1:4422858/1‑93 (MQ=255)

                                                              gTCCTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGcc                               <  2:3750563/93‑1 (MQ=255)

                                                                ccTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGa                             <  2:3085167/93‑1 (MQ=255)

                                                                ccTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGa                             <  2:4130006/93‑1 (MQ=255)

                                                                 cTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGAt                            <  1:6840388/93‑1 (MQ=255)

                                                                 cTGAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGAt                            <  2:1720963/93‑1 (MQ=255)

                                                                   gAAAACAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCg                          >  1:5511433/1‑93 (MQ=255)

                                                                     aaaCAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGtt                        >  1:738458/1‑93 (MQ=255)

                                                                     aaaCAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGtt                        <  1:1609724/93‑1 (MQ=255)

                                                                     aaaCAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGtt                        >  1:4579072/1‑93 (MQ=255)

                                                                       aCAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTc                      >  1:2794535/1‑93 (MQ=255)

                                                                       aCAGAGCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTC‑TACCTTTTGCCGCTATGATGCCGATCGTTTCt                     >  2:1556344/1‑93 (MQ=255)

                                                                         agagCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGGCGCTATGATGCCGATCGTTTCtt                    <  1:2332051/93‑1 (MQ=255)

                                                                         agagCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCtt                    >  2:590404/1‑93 (MQ=255)

                                                                           agCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGa                  >  2:695609/1‑93 (MQ=255)

                                                                           agCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGa                  <  1:6154087/93‑1 (MQ=255)

                                                                            gCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAg                 <  1:4262714/93‑1 (MQ=255)

                                                                            gCACACGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAg                 >  1:6048506/1‑93 (MQ=255)

                                                                               cacGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGtt              <  1:7097527/93‑1 (MQ=255)

                                                                               cacGTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGtt              >  2:4296579/1‑93 (MQ=255)

                                                                                  gTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatt           <  1:6103162/93‑1 (MQ=255)

                                                                                  gTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatt           <  1:996863/93‑1 (MQ=255)

                                                                                  gTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatt           <  1:4228195/93‑1 (MQ=255)

                                                                                  gTTAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatt           >  1:2179984/1‑93 (MQ=255)

                                                                                   ttAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta          <  1:1898032/93‑1 (MQ=255)

                                                                                   ttAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta          <  1:2724233/93‑1 (MQ=255)

                                                                                   ttAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta          >  2:129491/1‑93 (MQ=255)

                                                                                   ttAATAAGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta          <  1:3827320/93‑1 (MQ=255)

                                                                                   ttAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta          <  1:309734/93‑1 (MQ=255)

                                                                                     aataaGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTattatt        <  2:1920246/93‑1 (MQ=255)

                                                                                      ataaGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTc       >  2:699735/1‑93 (MQ=255)

                                                                                       taaGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCa      <  1:5179993/93‑1 (MQ=255)

                                                                                        aaGGCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAg     >  1:3516425/1‑93 (MQ=255)

                                                                                         aGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGt    >  2:4417465/1‑93 (MQ=255)

                                                                                           aCAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGttt  <  1:6785354/93‑1 (MQ=255)

CAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTT  >  NC_000913/1506972‑1507155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`