BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,523,536	T→C	T157A (ACC→GCC)	ansP ←	L‑asparagine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,523,536	0	T	C	85.6%	563.5 / 74.3	236	T157A (ACC→GCC)	ansP	L‑asparagine permease
Reads supporting (aligned to +/- strand): ref base T (26/8); new base C (102/100); total (128/108)
Fisher's exact test for biased strand distribution p-value = 5.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

CGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC  >  NC_000913/1523446‑1523623

cGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGc                                                                                       >  2:298660/1‑93 (MQ=255)

 gACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGcc                                                                                      >  1:3003694/1‑93 (MQ=255)

  aCCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCa                                                                                     >  1:6420482/1‑93 (MQ=255)

    cAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAAc                                                                                   >  2:2067692/1‑93 (MQ=255)

    cAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAAc                                                                                   >  1:2016419/1‑93 (MQ=255)

    cAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAAc                                                                                   >  1:5598956/1‑93 (MQ=255)

    cAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAAc                                                                                   >  1:5446081/1‑93 (MQ=255)

    cAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAAc                                                                                   <  1:4697538/93‑1 (MQ=255)

    cAAAAAGGTCACAAGGGCGAGCACTTTAATAAGCGCAAACCAGAGCTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAAc                                                                                   >  2:7488139/1‑93 (MQ=255)

     aaaaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACg                                                                                  >  2:1549669/1‑93 (MQ=255)

      aaaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGa                                                                                 >  2:3936033/1‑93 (MQ=255)

      aaaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGa                                                                                 <  2:6278839/93‑1 (MQ=255)

       aaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGAt                                                                                >  1:1152278/1‑93 (MQ=255)

       aaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGAt                                                                                <  2:61916/93‑1 (MQ=255)

       aaaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGAt                                                                                <  2:6006400/93‑1 (MQ=255)

        aaGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATg                                                                               >  1:7419984/1‑93 (MQ=255)

         aGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATgg                                                                              >  2:2241195/1‑93 (MQ=255)

         aGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATgg                                                                              <  2:3640943/93‑1 (MQ=255)

           gTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTa                                                                            >  2:2600584/1‑93 (MQ=255)

            tCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTaa                                                                           <  2:2544379/93‑1 (MQ=255)

            tCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTaa                                                                           <  2:1345963/93‑1 (MQ=255)

              acaATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGt                                                                         <  1:6157358/93‑1 (MQ=255)

              acaATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGt                                                                         <  2:4117018/93‑1 (MQ=255)

              acaATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGt                                                                         <  1:5169055/93‑1 (MQ=255)

                aaTGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGc                                                                       >  1:3721307/1‑93 (MQ=255)

                  tGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCag                                                                     >  1:4635485/1‑93 (MQ=255)

                   ggCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagc                                                                    <  1:6632189/93‑1 (MQ=255)

                     cGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcga                                                                  <  1:4969873/93‑1 (MQ=255)

                      gAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCagcgag                                                                 >  1:1334468/1‑93 (MQ=255)

                      gAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcgag                                                                 <  2:416411/93‑1 (MQ=255)

                       aGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcgagc                                                                >  2:5686131/1‑93 (MQ=255)

                       aGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcgagc                                                                >  1:1032501/1‑93 (MQ=255)

                        gCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAGACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcgagcg                                                               >  2:4347729/1‑93 (MQ=255)

                        gCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCagcgagcg                                                               >  2:4899671/1‑93 (MQ=255)

                         cACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAgcgc                                                              <  2:218704/93‑1 (MQ=255)

                         cACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAgcgc                                                              >  2:4611515/1‑93 (MQ=255)

                               aataaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGAc                                                        >  2:6734603/1‑93 (MQ=255)

                                ataaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGAcc                                                       >  2:4529691/1‑93 (MQ=255)

                                 taaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGAccc                                                      >  1:5951488/1‑93 (MQ=255)

                                  aaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCa                                                     >  2:6691094/1‑93 (MQ=255)

                                  aaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCa                                                     >  1:1604343/1‑93 (MQ=255)

                                  aaGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCa                                                     <  2:7088851/93‑1 (MQ=255)

                                   aGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCAc                                                    >  1:4202513/1‑93 (MQ=255)

                                   aGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCAc                                                    <  2:1354967/93‑1 (MQ=255)

                                   aGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCACCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCAc                                                    >  2:7373874/1‑93 (MQ=255)

                                     cgcAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTg                                                  <  2:3535433/93‑1 (MQ=255)

                                      gcAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGc                                                 <  1:228050/93‑1 (MQ=255)

                                           ccAGAGCTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAc                                            <  1:4836240/93‑1 (MQ=255)

                                             aGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgc                                          <  2:1480700/93‑1 (MQ=255)

                                             aGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgc                                          <  1:3170665/93‑1 (MQ=255)

                                             aGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgc                                          <  1:2723005/93‑1 (MQ=255)

                                             aGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgc                                          <  2:6120483/93‑1 (MQ=255)

                                              gAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgcc                                         <  2:646825/93‑1 (MQ=255)

                                              gAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgcc                                         >  2:1080009/1‑93 (MQ=255)

                                              gAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgcc                                         >  1:6567814/1‑93 (MQ=255)

                                                aCTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgccgc                                       >  2:2840229/1‑93 (MQ=255)

                                                aCTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCAcgccgc                                       >  1:7080339/1‑93 (MQ=255)

                                                 cTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACgccgcc                                      >  2:1104427/1‑93 (MQ=255)

                                                  tCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCa                                     >  1:3901752/1‑93 (MQ=255)

                                                  tCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCa                                     <  1:4113713/93‑1 (MQ=255)

                                                  tCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCa                                     >  2:5482225/1‑93 (MQ=255)

                                                  tCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCa                                     >  1:2875635/1‑93 (MQ=255)

                                                   ccATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCaa                                    <  2:2057091/93‑1 (MQ=255)

                                                    cATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCaaa                                   <  1:4052096/93‑1 (MQ=255)

                                                    cATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCaaa                                   <  2:6923056/93‑1 (MQ=255)

                                                    cATCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCaaa                                   <  1:6552686/93‑1 (MQ=255)

                                                     aTCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAAc                                  <  2:6890349/93‑1 (MQ=255)

                                                     aTCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCGGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAAc                                  >  2:3153609/1‑93 (MQ=255)

                                                     aTCTCCGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAAc                                  <  2:7224732/93‑1 (MQ=255)

                                                         ccGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCAc                              >  1:3237908/1‑93 (MQ=255)

                                                          cGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCAcc                             >  1:5834580/1‑93 (MQ=255)

                                                          cGCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCAcc                             >  1:2186602/1‑93 (MQ=255)

                                                           gCAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCAccc                            >  1:3840404/1‑93 (MQ=255)

                                                            cAAACCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCAcccc                           >  1:4408721/1‑93 (MQ=255)

                                                             aaaCCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCa                          >  2:2789473/1‑93 (MQ=255)

                                                               aCCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGt                        >  1:7410196/1‑93 (MQ=255)

                                                               aCCATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGt                        <  2:1017616/93‑1 (MQ=255)

                                                                 cATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTaa                      >  2:6359156/1‑93 (MQ=255)

                                                                 cATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTaa                      >  1:6004027/1‑93 (MQ=255)

                                                                 cATTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTaa                      <  2:27508/93‑1 (MQ=255)

                                                                  aTTTCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAAt                     <  2:2404698/93‑1 (MQ=255)

                                                                     tCACACCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCa                  <  1:2397676/93‑1 (MQ=255)

                                                                      cgcacCGAGCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCCCTGCGGCACGCCGCCAAACGCACCCCAGTAATGCat                 <  1:6023805/91‑1 (MQ=255)

                                                                       acacCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCata                <  1:569461/93‑1 (MQ=255)

                                                                       acacCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCata                <  2:7196368/93‑1 (MQ=255)

                                                                       acacCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCata                <  2:3109759/93‑1 (MQ=255)

                                                                        cacCGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCatat               >  2:7229615/1‑93 (MQ=255)

                                                                          ccGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATAc             <  1:3376990/93‑1 (MQ=255)

                                                                           cGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACa            <  2:339888/93‑1 (MQ=255)

                                                                           cGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACa            <  1:1705624/93‑1 (MQ=255)

                                                                           cGATCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACa            <  2:1650942/93‑1 (MQ=255)

                                                                             aTCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACaga          <  2:80578/93‑1 (MQ=255)

                                                                             aTCATATTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACaga          >  1:4532197/1‑93 (MQ=255)

                                                                                atatTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCg       >  1:7056660/1‑93 (MQ=255)

                                                                                 tatTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGa      >  2:1456056/1‑93 (MQ=255)

                                                                                 tatTCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGa      >  2:3185585/1‑93 (MQ=255)

                                                                                  atTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGAc     >  2:4390572/1‑93 (MQ=255)

                                                                                   ttCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGAcg    >  2:463721/1‑93 (MQ=255)

                                                                                   ttCATGGCGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGAcg    <  1:2671211/93‑1 (MQ=255)

                                                                                   ttCATGGCGCCAACCATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGAcg    >  2:6442487/1‑93 (MQ=255)

CGACCAAAAAGGTCACAATGGCGAGCACTTTAATAAGCGCAAACCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGC  >  NC_000913/1523446‑1523623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`