BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,571,055	T→C	L724L (TTA→TTG)	pqqL ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,571,055	0	T	C	88.4%	785.8 / 83.6	303	L724L (TTA→TTG)	pqqL	putative peptidase
Reads supporting (aligned to +/- strand): ref base T (17/18); new base C (133/135); total (150/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

Only 100 of 303 total aligned reads displayed.

TTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAATTGG  >  NC_000913/1570965‑1571145

 tGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAg                                                                                         >  1:1780308/1‑93 (MQ=255)

  gTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGt                                                                                        >  2:1920244/1‑93 (MQ=255)

  gTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGt                                                                                        >  2:2832368/1‑93 (MQ=255)

  gTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGt                                                                                        <  1:2823058/93‑1 (MQ=255)

     cTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAAc                                                                                     >  1:6752537/1‑93 (MQ=255)

      ttttACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAAcg                                                                                    <  2:7154986/93‑1 (MQ=255)

      ttttACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAAcg                                                                                    >  1:2456388/1‑93 (MQ=255)

       tttACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAAcgc                                                                                   >  2:2283820/1‑93 (MQ=255)

        ttACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAAcgcg                                                                                  <  2:639392/93‑1 (MQ=255)

         tACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGt                                                                                 >  1:3803534/1‑93 (MQ=255)

          aCAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTa                                                                                >  2:6880649/1‑93 (MQ=255)

          aCAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTa                                                                                >  2:1477006/1‑93 (MQ=255)

           cAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTaa                                                                               <  2:5200942/93‑1 (MQ=255)

            aGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAAt                                                                              >  2:739040/1‑93 (MQ=255)

             gTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAAtt                                                                             >  1:4531527/1‑93 (MQ=255)

             gTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAAtt                                                                             >  1:2958114/1‑93 (MQ=255)

              tAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTa                                                                            <  1:5082455/93‑1 (MQ=255)

              tAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTa                                                                            <  2:3298055/93‑1 (MQ=255)

              tAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTa                                                                            >  2:3364666/1‑93 (MQ=255)

               aaCCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTaa                                                                           <  2:4290148/93‑1 (MQ=255)

                aCCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAAc                                                                          >  1:1951776/1‑93 (MQ=255)

                 ccGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACg                                                                         <  2:3912933/93‑1 (MQ=255)

                    aTGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCa                                                                      <  1:6881236/93‑1 (MQ=255)

                    aTGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCa                                                                      >  2:824899/1‑93 (MQ=255)

                      gCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACg                                                                    >  2:151271/1‑93 (MQ=255)

                       cGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGa                                                                   <  1:438447/93‑1 (MQ=255)

                        gTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAg                                                                  <  2:6827563/93‑1 (MQ=255)

                         ttGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGt                                                                 >  1:762924/1‑93 (MQ=255)

                         ttGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGt                                                                 <  1:4063044/93‑1 (MQ=255)

                           gTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGttt                                                               <  1:3854821/93‑1 (MQ=255)

                           gTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGttt                                                               <  2:5486947/93‑1 (MQ=255)

                           gTCCGACGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGttt                                                               >  1:4698691/1‑93 (MQ=255)

                              cGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtc                                                            <  2:7505493/93‑1 (MQ=255)

                               gTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtct                                                           >  2:2014345/1‑93 (MQ=255)

                               gTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtct                                                           >  2:5656065/1‑93 (MQ=255)

                                tCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtctt                                                          >  1:2079721/1‑93 (MQ=255)

                                tCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtctt                                                          >  1:6411000/1‑93 (MQ=255)

                                tCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGGGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtctt                                                          >  1:5337011/1‑93 (MQ=255)

                                 cgcgcgAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGtcttc                                                         <  1:7185294/93‑1 (MQ=255)

                                 cgcgcgAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtcttc                                                         <  1:1017248/93‑1 (MQ=255)

                                 cgcgcgAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGtcttc                                                         <  1:4438280/93‑1 (MQ=255)

                                   cgcgAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTg                                                       >  1:2206236/1‑93 (MQ=255)

                                    gcgAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGc                                                      <  1:4594285/93‑1 (MQ=255)

                                      gAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGa                                                    >  2:379865/1‑93 (MQ=255)

                                       aGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGAc                                                   <  1:2252623/93‑1 (MQ=255)

                                        gTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACa                                                  <  1:4865029/93‑1 (MQ=255)

                                        gTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACa                                                  >  2:6549498/1‑93 (MQ=255)

                                           aaTGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTa                                               >  2:1213174/1‑93 (MQ=255)

                                            aTGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTAc                                              <  2:2908791/93‑1 (MQ=255)

                                            aTGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTAc                                              <  2:1085149/93‑1 (MQ=255)

                                            aTGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTAc                                              >  1:1065052/1‑93 (MQ=255)

                                             tGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTAcc                                             <  1:3992357/93‑1 (MQ=255)

                                               gTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGa                                           >  2:6747475/1‑93 (MQ=255)

                                                 ttACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACa                                         <  2:4607452/93‑1 (MQ=255)

                                                 ttACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACa                                         >  2:4129607/1‑93 (MQ=255)

                                                  tACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACaa                                        <  1:5826658/93‑1 (MQ=255)

                                                  tACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACaa                                        <  1:5448597/93‑1 (MQ=255)

                                                   aCCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAAt                                       <  1:1929484/93‑1 (MQ=255)

                                                    ccTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATg                                      >  1:2290388/1‑93 (MQ=255)

                                                       gCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACa                                   >  2:4847598/1‑93 (MQ=255)

                                                       gCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACa                                   >  1:3196043/1‑93 (MQ=255)

                                                         ggCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACaaa                                 <  1:4216444/93‑1 (MQ=255)

                                                         ggCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACaaa                                 >  1:5526327/1‑93 (MQ=255)

                                                         ggCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACaaa                                 <  2:4366481/93‑1 (MQ=255)

                                                          gCTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAAc                                >  2:2456449/1‑93 (MQ=255)

                                                           cTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACg                               <  1:6589949/93‑1 (MQ=255)

                                                           cTAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACg                               >  2:5734784/1‑93 (MQ=255)

                                                            tAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGt                              >  1:2747640/1‑93 (MQ=255)

                                                            tAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGt                              <  2:1799982/93‑1 (MQ=255)

                                                            tAATGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGt                              <  1:1248636/93‑1 (MQ=255)

                                                             aaTGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTg                             >  2:1768489/1‑93 (MQ=255)

                                                              aTGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGa                            <  2:6992381/93‑1 (MQ=255)

                                                              aTGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGa                            <  1:873666/93‑1 (MQ=255)

                                                               tGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGat                           >  1:6274527/1‑93 (MQ=255)

                                                               tGGCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGat                           <  2:7248638/93‑1 (MQ=255)

                                                                ggCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGata                          <  1:6325891/93‑1 (MQ=255)

                                                                ggCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGata                          <  2:2232500/93‑1 (MQ=255)

                                                                ggCGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGata                          <  2:1563623/93‑1 (MQ=255)

                                                                  cGAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATc                        <  1:5749006/93‑1 (MQ=255)

                                                                   gAATCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATcc                       <  2:6937156/93‑1 (MQ=255)

                                                                    aaTCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCg                      >  2:5150293/1‑93 (MQ=255)

                                                                      tCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCt                    <  1:3466908/93‑1 (MQ=255)

                                                                      tCAGAGTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCt                    >  1:1519844/1‑93 (MQ=255)

                                                                        agagTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTgg                  >  1:7428612/1‑93 (MQ=255)

                                                                        agagTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTgg                  >  1:6788127/1‑93 (MQ=255)

                                                                         gagTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGga                 >  2:619160/1‑93 (MQ=255)

                                                                         gagTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGga                 <  1:1426024/93‑1 (MQ=255)

                                                                         gagTGTTTGATTGATCCCAAGTAACGCATAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCTGCTGga                 >  2:5008007/1‑93 (MQ=255)

                                                                          agTGTTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGgag                <  2:5807689/93‑1 (MQ=255)

                                                                           gtgtTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGgaga               >  1:5192971/1‑93 (MQ=255)

                                                                            tgtTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGAt              <  2:6138703/93‑1 (MQ=255)

                                                                            tgtTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGAt              >  1:6149779/1‑93 (MQ=255)

                                                                            tgtTTGATTGATCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGAt              <  1:4311808/93‑1 (MQ=255)

                                                                                tgattgatCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGaaa          >  1:6012455/1‑93 (MQ=255)

                                                                                 gattgatCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCCGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGaaaa         >  2:2110796/1‑93 (MQ=255)

                                                                                   ttgatCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACa       >  2:3496805/1‑93 (MQ=255)

                                                                                     gatCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAAt     <  2:6670885/93‑1 (MQ=255)

                                                                                      atCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAAtt    >  2:5373024/1‑93 (MQ=255)

                                                                                      atCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAAtt    >  1:4188869/1‑93 (MQ=255)

                                                                                       tCCCAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAATTg   <  1:4806025/93‑1 (MQ=255)

TTGTTCTTTTACAGTAACCGATGCGTTGTCCGTCGCGCGAGTTAATGGTTTACCTGCGGCTAATGGCGAATCAGAGTGTTTGATTGATCCTAAGTAACGCGTAATTAACGCCACGAGTTTGTCTTCTGCGACATTACCGACAATGACAAACGTGATATCCGCTGGAGATGAAAACAATTGG  >  NC_000913/1570965‑1571145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`