BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,592,738	T→C	T1125A (ACG→GCG)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,592,738	0	T	C	86.6%	571.4 / 75.8	239	T1125A (ACG→GCG)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (17/15); new base C (97/110); total (114/125)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 239 total aligned reads displayed.

CCCCTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCTTTGAACA  >  NC_000913/1592649‑1592827

ccccTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTcc                                                                                        >  1:5855510/1‑93 (MQ=255)

ccccTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTcc                                                                                        >  2:299542/1‑93 (MQ=255)

 cccTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccc                                                                                       >  1:3683713/1‑93 (MQ=255)

   cTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccccc                                                                                     <  1:3552394/93‑1 (MQ=255)

   cTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccccc                                                                                     <  2:4575464/93‑1 (MQ=255)

   cTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccccc                                                                                     <  1:1362056/93‑1 (MQ=255)

     gAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTg                                                                                   <  1:4625784/93‑1 (MQ=255)

     gAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTg                                                                                   <  2:2200675/93‑1 (MQ=255)

      aGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGt                                                                                  <  2:6578472/93‑1 (MQ=255)

          ttatCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccccc                                                                                     >  2:6108185/1‑86 (MQ=255)

          ttatCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTccccc                                                                                     <  1:6108185/86‑1 (MQ=255)

          ttatCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACa                                                                              <  2:7127814/93‑1 (MQ=255)

           tatCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACAt                                                                             >  1:7506661/1‑93 (MQ=255)

           tatCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACAt                                                                             <  1:712805/93‑1 (MQ=255)

             tCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTc                                                                           >  2:3652526/1‑93 (MQ=255)

              cGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCa                                                                          <  1:7009739/93‑1 (MQ=255)

              cGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCa                                                                          <  2:3740380/93‑1 (MQ=255)

              cGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCa                                                                          <  1:1203812/93‑1 (MQ=255)

               gAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAg                                                                         <  1:2367570/93‑1 (MQ=255)

                aGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAgg                                                                        >  2:5190805/1‑93 (MQ=255)

                 ggCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGt                                                                       >  2:3914558/1‑93 (MQ=255)

                 ggCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGt                                                                       >  2:3007293/1‑93 (MQ=255)

                  gCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGtt                                                                      <  2:5840120/93‑1 (MQ=255)

                  gCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGtt                                                                      <  1:3457599/93‑1 (MQ=255)

                  gCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGtt                                                                      >  1:1074043/1‑93 (MQ=255)

                    tGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTc                                                                    <  2:4664653/93‑1 (MQ=255)

                    tGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTc                                                                    >  1:6732792/1‑93 (MQ=255)

                     gACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTcc                                                                   <  2:6879419/93‑1 (MQ=255)

                       cTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGc                                                                 >  1:3299309/1‑93 (MQ=255)

                       cTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGc                                                                 >  1:5304652/1‑93 (MQ=255)

                        ttCCGCGTTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGcc                                                                >  1:1045627/1‑93 (MQ=255)

                         tCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCa                                                               <  1:5712734/93‑1 (MQ=255)

                              aTTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGt                                                          <  1:590528/93‑1 (MQ=255)

                              aTTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGt                                                          >  1:3190118/1‑93 (MQ=255)

                               tttGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTc                                                         >  2:1668974/1‑93 (MQ=255)

                               tttGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTc                                                         <  1:5973782/93‑1 (MQ=255)

                                ttGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCa                                                        <  1:174950/93‑1 (MQ=255)

                                  gggCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGt                                                      >  2:6971456/1‑93 (MQ=255)

                                   ggCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGtt                                                     >  1:3366030/1‑93 (MQ=255)

                                    gCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTc                                                    >  2:5616074/1‑93 (MQ=255)

                                      tAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAc                                                  <  1:4931252/93‑1 (MQ=255)

                                      tAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAc                                                  >  2:7069269/1‑93 (MQ=255)

                                       aaTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAcc                                                 <  1:2298875/93‑1 (MQ=255)

                                       aaTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAcc                                                 >  1:1012660/1‑93 (MQ=255)

                                       aaTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAcc                                                 <  2:2055564/93‑1 (MQ=255)

                                       aaTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAcc                                                 <  1:6388117/93‑1 (MQ=255)

                                       aaTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAcc                                                 <  2:5342259/93‑1 (MQ=255)

                                        aTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCAccc                                                <  2:7313437/93‑1 (MQ=255)

                                        aTGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATCCAGGTTTCCGCCACCTGTCAGTTCAccc                                                <  1:4048527/93‑1 (MQ=255)

                                         tGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCct                                               >  2:1375171/1‑93 (MQ=255)

                                            tGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgac                                            <  2:1565406/93‑1 (MQ=255)

                                            tGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgac                                            >  2:2330106/1‑93 (MQ=255)

                                             ggTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgact                                           <  2:3536136/93‑1 (MQ=255)

                                              gTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgactg                                          <  2:4778757/93‑1 (MQ=255)

                                              gTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgactg                                          <  2:2747895/93‑1 (MQ=255)

                                               ttAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgactga                                         >  1:4306581/1‑93 (MQ=255)

                                               ttAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgactga                                         <  2:1870213/93‑1 (MQ=255)

                                               ttAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCctgactga                                         <  2:6351299/93‑1 (MQ=255)

                                                tAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGAt                                        >  2:4146262/1‑93 (MQ=255)

                                                  aCGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGCAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATcc                                      >  2:7318494/1‑93 (MQ=255)

                                                  aCGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATcc                                      >  2:2268014/1‑93 (MQ=255)

                                                  aCGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCCCAACCTTCCAGTTCACCTTGCTTGATcc                                      >  1:721216/1‑93 (MQ=255)

                                                   cGCATTGTAGCGTGCATTAAGCCCTTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATccc                                     <  2:4783856/93‑1 (MQ=255)

                                                    gCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCa                                    >  1:1417315/1‑93 (MQ=255)

                                                    gCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCa                                    <  1:5127594/93‑1 (MQ=255)

                                                      aTTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCAcc                                  <  2:3041409/93‑1 (MQ=255)

                                                       ttGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCt                                 <  2:2008953/93‑1 (MQ=255)

                                                       ttGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCt                                 >  2:6267055/1‑93 (MQ=255)

                                                        tGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCtt                                <  1:2183091/93‑1 (MQ=255)

                                                          tAGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCttta                              <  2:2586918/93‑1 (MQ=255)

                                                           aGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCtttat                             <  1:1581481/93‑1 (MQ=255)

                                                           aGCGTGCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCtttat                             <  2:5410147/93‑1 (MQ=255)

                                                                gCATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTAcc                        >  2:3335392/1‑93 (MQ=255)

                                                                 cATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCg                       >  1:990410/1‑93 (MQ=255)

                                                                 cATTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCg                       >  1:4248407/1‑93 (MQ=255)

                                                                  aTTAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGt                      >  2:6516403/1‑93 (MQ=255)

                                                                   ttAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTc                     <  1:5087895/93‑1 (MQ=255)

                                                                   ttAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTc                     >  2:5569250/1‑93 (MQ=255)

                                                                    tAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCa                    <  2:696213/93‑1 (MQ=255)

                                                                    tAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCa                    <  1:3524539/93‑1 (MQ=255)

                                                                    tAAGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCa                    <  2:1814167/93‑1 (MQ=255)

                                                                      aGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAgc                  <  1:2832896/93‑1 (MQ=255)

                                                                      aGCCCCTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAgc                  >  2:1090472/1‑93 (MQ=255)

                                                                      aGCCCCTCGATAGCCAGCGCGCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAgc                  <  1:4711924/93‑1 (MQ=255)

                                                                        ccccTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAgcgc                <  2:5749582/93‑1 (MQ=255)

                                                                          ccTCGATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGccc              <  2:4416933/93‑1 (MQ=255)

                                                                            tCGATAGCCAGCGTTCCCCCTGCAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGccccc            >  2:848572/1‑93 (MQ=255)

                                                                              gATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCctc          <  2:4730364/93‑1 (MQ=255)

                                                                              gATAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCctc          >  1:5575929/1‑93 (MQ=255)

                                                                               aTAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCctct         <  2:2618669/93‑1 (MQ=255)

                                                                                tAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCtt        >  1:3808904/1‑93 (MQ=255)

                                                                                tAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCtt        >  1:5992253/1‑93 (MQ=255)

                                                                                tAGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCtt        >  1:7157719/1‑93 (MQ=255)

                                                                                 aGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCttt       >  2:6658352/1‑93 (MQ=255)

                                                                                 aGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCTCCTTTATTTACCGTCAGCGCCCCCTCttt       <  1:6592634/93‑1 (MQ=255)

                                                                                 aGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCttt       <  1:1978418/93‑1 (MQ=255)

                                                                                 aGCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCttt       <  2:7520118/93‑1 (MQ=255)

                                                                                  gCCAGCGTTCCCCCTGCAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCTTTg      >  2:884426/1‑93 (MQ=255)

                                                                                  gCCAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCTTTg      <  1:2489363/93‑1 (MQ=255)

                                                                                    cAGCGCTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCTTTGaa    >  1:3025049/1‑93 (MQ=255)

CCCCTGAGTATTATCGAGGCTGACTTCCGCATTTGGGCTAATGCTGGTTAACGCATTGTAGCGTGCATTAAGCCCCTCGATAGCCAGCGTTCCCCCTGTAACATTCAGGTTTCCGCCACCTGTCAGTTCACCCTGACTGATCCCACCTTTATTTACCGTCAGCGCCCCCTCTTTGAACA  >  NC_000913/1592649‑1592827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`