BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,400	T→C	L97P (CTG→CCG)	lhr →	Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,400	0	T	C	88.0%	603.7 / 69.8	241	L97P (CTG→CCG)	lhr	Lhr
Reads supporting (aligned to +/- strand): ref base T (17/12); new base C (113/99); total (130/111)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

Only 100 of 245 total aligned reads displayed.

GATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCG  >  NC_000913/1727309‑1727492

gATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTg                                                                                             <  2:2065342/93‑1 (MQ=255)

 aTCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTgg                                                                                            <  2:7116333/93‑1 (MQ=255)

 aTCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTgg                                                                                            >  2:5285017/1‑93 (MQ=255)

 aTCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCgg                                                                                            <  1:6782255/93‑1 (MQ=255)

 aTCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCgg                                                                                            <  2:7330955/93‑1 (MQ=255)

  tCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTggg                                                                                           <  2:4022578/93‑1 (MQ=255)

  tCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCggg                                                                                           >  1:1749859/1‑93 (MQ=255)

    ggCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCa                                                                                         >  1:5154221/1‑93 (MQ=255)

    ggCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCa                                                                                         >  1:5877363/1‑93 (MQ=255)

    ggCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCa                                                                                         >  2:4237807/1‑93 (MQ=255)

        ctTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGa                                                                                     >  2:4969996/1‑93 (MQ=255)

        ctTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGa                                                                                     <  1:5538305/93‑1 (MQ=255)

         ttCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGAc                                                                                    <  1:2031734/93‑1 (MQ=255)

         ttCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGAc                                                                                    <  1:2801117/93‑1 (MQ=255)

         ttCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGAc                                                                                    <  1:369295/93‑1 (MQ=255)

          tCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACg                                                                                   <  1:4413047/93‑1 (MQ=255)

             gcgAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTc                                                                                <  1:1008593/93‑1 (MQ=255)

              cgAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCa                                                                               <  2:2656878/93‑1 (MQ=255)

                  ggcggcGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAgcgc                                                                           >  1:1580948/1‑93 (MQ=255)

                      gcgAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACt                                                                       <  1:7169841/93‑1 (MQ=255)

                        gAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTg                                                                     >  2:391325/1‑93 (MQ=255)

                         aaGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGc                                                                    <  1:692504/93‑1 (MQ=255)

                         aaGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGc                                                                    >  1:631791/1‑93 (MQ=255)

                          aGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCa                                                                   >  1:2992051/1‑93 (MQ=255)

                          aGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCa                                                                   >  2:6150269/1‑93 (MQ=255)

                           gATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCTCCGGGCACCGACGTTCAGCGCAACTTGCAg                                                                  >  2:950143/1‑93 (MQ=255)

                           gATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAg                                                                  >  1:2324186/1‑93 (MQ=255)

                             tACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGAt                                                                >  1:1800854/1‑93 (MQ=255)

                              aCCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATc                                                               <  1:5356812/93‑1 (MQ=255)

                                ccGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATccc                                                             >  1:7409347/1‑93 (MQ=255)

                                ccGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATccc                                                             <  1:2438475/93‑1 (MQ=255)

                                ccGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATccc                                                             <  2:1953543/93‑1 (MQ=255)

                                  gcgAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGt                                                           <  2:5886561/93‑1 (MQ=255)

                                   cgAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGtt                                                          <  1:4179769/93‑1 (MQ=255)

                                   cgAGGCGCATAAGAGTAAAACCGCACGCATCCTCTAAATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGtt                                                          <  1:2595079/93‑1 (MQ=255)

                                      ggCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGaa                                                       >  2:3232720/1‑93 (MQ=255)

                                      ggCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGaa                                                       >  2:5570115/1‑93 (MQ=255)

                                       gcgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAg                                                      <  2:1238958/93‑1 (MQ=255)

                                       gcgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGAACCCGTTGAAg                                                      <  1:1476224/93‑1 (MQ=255)

                                        cgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAgg                                                     <  1:6105068/93‑1 (MQ=255)

                                        cgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAgg                                                     <  1:955754/93‑1 (MQ=255)

                                        cgcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAgg                                                     <  1:6474378/93‑1 (MQ=255)

                                         gcATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAggg                                                    <  2:6506996/93‑1 (MQ=255)

                                          cATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGt                                                   >  1:4062691/1‑93 (MQ=255)

                                          cATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGt                                                   >  2:2678086/1‑93 (MQ=255)

                                          cATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGt                                                   >  2:7064589/1‑93 (MQ=255)

                                           aTAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTa                                                  <  2:4674738/93‑1 (MQ=255)

                                           aTAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTa                                                  <  2:1426623/93‑1 (MQ=255)

                                           aTAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTa                                                  <  2:6747529/93‑1 (MQ=255)

                                            tAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTAt                                                 <  2:2920981/93‑1 (MQ=255)

                                            tAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTAt                                                 >  1:3261321/1‑93 (MQ=255)

                                            tAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTAt                                                 >  2:1766764/1‑93 (MQ=255)

                                            tAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTAt                                                 >  1:4573838/1‑93 (MQ=255)

                                             aaGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTAtt                                                >  2:3822460/1‑93 (MQ=255)

                                              aGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTg                                               >  1:1186755/1‑93 (MQ=255)

                                                cGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGcc                                             >  2:5753278/1‑93 (MQ=255)

                                                  tAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGa                                           >  2:5655481/1‑93 (MQ=255)

                                                     aaCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGa                                        >  2:497870/1‑93 (MQ=255)

                                                      aCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGaa                                       <  2:2439142/93‑1 (MQ=255)

                                                      aCCTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGaa                                       <  1:6401328/93‑1 (MQ=255)

                                                       ccTCACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAc                                      >  1:1268956/1‑93 (MQ=255)

                                                          cACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggc                                   <  1:7197310/93‑1 (MQ=255)

                                                          cACGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggc                                   <  2:7281972/93‑1 (MQ=255)

                                                            cGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcgg                                 <  1:5582119/93‑1 (MQ=255)

                                                            cGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcgg                                 >  1:3602938/1‑93 (MQ=255)

                                                            cGCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcgg                                 <  1:222455/93‑1 (MQ=255)

                                                             gCATCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcggc                                <  1:5197275/93‑1 (MQ=255)

                                                               aTCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcggcgg                              >  2:4762834/1‑93 (MQ=255)

                                                               aTCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAAcggcggcgg                              >  1:3134696/1‑93 (MQ=255)

                                                                tCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACggcggcggc                             >  1:4806249/1‑93 (MQ=255)

                                                                tCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACggcggcggc                             >  2:3677389/1‑93 (MQ=255)

                                                                tCCTCTATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACggcggcggc                             >  2:536366/1‑93 (MQ=255)

                                                                  ctctATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgc                           >  2:4433488/1‑93 (MQ=255)

                                                                   tctATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgcg                          >  2:6112961/1‑93 (MQ=255)

                                                                   tctATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgcg                          <  2:2762484/93‑1 (MQ=255)

                                                                   tctATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgcg                          >  2:7386025/1‑93 (MQ=255)

                                                                   tctATATTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgcg                          <  1:7030045/93‑1 (MQ=255)

                                                                     tataTTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGgcggcgcggc                        >  1:7442360/1‑93 (MQ=255)

                                                                        atTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGaa                     >  1:1356690/1‑93 (MQ=255)

                                                                        atTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGaa                     >  1:3726682/1‑93 (MQ=255)

                                                                        atTTCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGaa                     >  2:4479807/1‑93 (MQ=255)

                                                                         tttCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGaaa                    >  2:2244082/1‑93 (MQ=255)

                                                                           tCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACg                  >  1:4336551/1‑93 (MQ=255)

                                                                           tCACCGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACg                  <  1:13865/93‑1 (MQ=255)

                                                                             aCCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGa                <  1:6576697/93‑1 (MQ=255)

                                                                               cGATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAg              >  2:2366260/1‑93 (MQ=255)

                                                                                gATAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGt             <  1:5680186/93‑1 (MQ=255)

                                                                                 aTAAAAGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTc            >  1:6202009/1‑93 (MQ=255)

                                                                                   aaaaGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCaa          >  1:3334151/1‑93 (MQ=255)

                                                                                    aaaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAAt         >  1:6913683/1‑93 (MQ=255)

                                                                                    aaaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAAt         <  1:3476513/93‑1 (MQ=255)

                                                                                     aaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATc        >  2:6124375/1‑93 (MQ=255)

                                                                                     aaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATc        <  2:4815195/93‑1 (MQ=255)

                                                                                     aaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATc        >  2:3551472/1‑93 (MQ=255)

                                                                                     aaGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATc        >  1:1982625/1‑93 (MQ=255)

                                                                                      aGCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCt       >  1:1945183/1‑93 (MQ=255)

                                                                                       gCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCtt      <  1:6823116/93‑1 (MQ=255)

                                                                                       gCCCCGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCtt      <  1:5337534/93‑1 (MQ=255)

                                                                                        ccccGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTc     <  1:2192458/93‑1 (MQ=255)

                                                                                           tGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGTGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTcgcg  >  1:2547410/1‑93 (MQ=255)

GATCGGCTCTTCCGCGAAGGCGGCGAAGATACCCGCGAGGCGCATAAGCGTAAAACCTCACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCG  >  NC_000913/1727309‑1727492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`