BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	144,241	A→G	P180P (CCA→CCG)	yadH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,241	0	A	G	84.0%	608.7 / 103.9	268	P180P (CCA→CCG)	yadH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (21/22); new base G (118/107); total (139/129)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 272 total aligned reads displayed.

GGTGTTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGAT  >  NC_000913/144151‑144333

ggTGTTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCt                                                                                            >  1:1464449/1‑93 (MQ=255)

 gtgtTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTc                                                                                           >  1:3895902/1‑93 (MQ=255)

 gtgtTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTc                                                                                           >  2:2777818/1‑93 (MQ=255)

 gtgtTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTc                                                                                           <  1:4657579/93‑1 (MQ=255)

  tgttgtTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCa                                                                                          <  1:55932/93‑1 (MQ=255)

  tgttgtTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCa                                                                                          <  2:1608181/93‑1 (MQ=255)

  tgttgtTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCa                                                                                          <  1:4697682/93‑1 (MQ=255)

   gttgttCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCAc                                                                                         <  1:1425133/93‑1 (MQ=255)

   gttgttCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCAc                                                                                         >  1:5706467/1‑93 (MQ=255)

      gttCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTa                                                                                      <  2:958287/93‑1 (MQ=255)

        tctcCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTAtt                                                                                    <  1:3979380/93‑1 (MQ=255)

        tctcCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTAtt                                                                                    >  1:3084610/1‑93 (MQ=255)

         ctcCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTAttt                                                                                   >  2:4294216/1‑93 (MQ=255)

         ctcCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTAttt                                                                                   <  2:223555/93‑1 (MQ=255)

              ttGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCg                                                                              >  1:6428859/1‑93 (MQ=255)

              ttGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCg                                                                              >  2:5568234/1‑93 (MQ=255)

                gCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCggg                                                                            >  1:1042520/1‑93 (MQ=255)

                gCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCggg                                                                            <  1:4897349/93‑1 (MQ=255)

                 cGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCgggg                                                                           >  2:7118565/1‑93 (MQ=255)

                 cGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCgggg                                                                           <  2:4604485/93‑1 (MQ=255)

                   ggTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTc                                                                         >  1:5067082/1‑93 (MQ=255)

                    gTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCt                                                                        >  1:4510780/1‑93 (MQ=255)

                    gTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCt                                                                        <  2:7194580/93‑1 (MQ=255)

                    gTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCt                                                                        <  1:1881685/93‑1 (MQ=255)

                     tttGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCtt                                                                       >  2:6172287/1‑93 (MQ=255)

                     tttGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCtt                                                                       <  2:4501599/93‑1 (MQ=255)

                      ttGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCttt                                                                      <  2:3865672/93‑1 (MQ=255)

                        gCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTa                                                                    >  2:2881168/1‑93 (MQ=255)

                        gCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTa                                                                    >  1:4681576/1‑93 (MQ=255)

                          tGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACt                                                                  >  2:374348/1‑93 (MQ=255)

                          tGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACt                                                                  >  1:5445041/1‑93 (MQ=255)

                          tGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACt                                                                  >  1:3660561/1‑93 (MQ=255)

                           gAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTc                                                                 >  1:5663231/1‑93 (MQ=255)

                            aaCGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCa                                                                <  1:2001900/93‑1 (MQ=255)

                             aCGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCAc                                                               <  1:5893453/93‑1 (MQ=255)

                               ggTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTg                                                             >  1:3551828/1‑93 (MQ=255)

                               ggTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTg                                                             <  1:6657355/93‑1 (MQ=255)

                                gtgtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGa                                                            <  2:1030109/93‑1 (MQ=255)

                                gtgtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGa                                                            <  2:1765473/93‑1 (MQ=255)

                                 tgtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGAc                                                           <  2:1388693/93‑1 (MQ=255)

                                 tgtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGAc                                                           >  1:2558400/1‑93 (MQ=255)

                                  gtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACt                                                          >  1:375195/1‑93 (MQ=255)

                                  gtgtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACt                                                          >  1:344336/1‑93 (MQ=255)

                                    gtTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACttt                                                        >  1:5108567/1‑93 (MQ=255)

                                     tttGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTttg                                                       >  2:3057118/1‑93 (MQ=255)

                                     tttGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTttg                                                       >  2:2038166/1‑93 (MQ=255)

                                     tttGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTttg                                                       >  1:2263329/1‑93 (MQ=255)

                                      ttGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTttgt                                                      <  2:7035443/93‑1 (MQ=255)

                                       tGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTttgtt                                                     >  2:2906384/1‑93 (MQ=255)

                                         ccAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgc                                                   >  1:7502305/1‑93 (MQ=255)

                                         ccAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgc                                                   <  2:6854721/93‑1 (MQ=255)

                                         ccAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgc                                                   >  2:6347309/1‑93 (MQ=255)

                                          cAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTCACTTTGTTgcc                                                  <  1:131977/93‑1 (MQ=255)

                                          cAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgcc                                                  >  2:2207949/1‑93 (MQ=255)

                                          cAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgcc                                                  <  1:7266964/93‑1 (MQ=255)

                                            aaaCGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgccgc                                                >  2:6540764/1‑93 (MQ=255)

                                            aaaCGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGCCTTTTACTCACTGACTTTGTTgccgc                                                >  2:2069763/1‑93 (MQ=255)

                                             aaCGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgccgcc                                               >  1:2719099/1‑93 (MQ=255)

                                             aaCGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTgccgcc                                               >  2:3357209/1‑93 (MQ=255)

                                                  tCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCt                                          <  2:3565121/93‑1 (MQ=255)

                                                    gATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTgg                                        >  1:1114760/1‑93 (MQ=255)

                                                    gATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTgg                                        >  2:1000414/1‑93 (MQ=255)

                                                    gATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTgg                                        >  1:5607098/1‑93 (MQ=255)

                                                    gATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTgg                                        >  1:47704/1‑93 (MQ=255)

                                                     aTGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGc                                       >  2:1424385/1‑93 (MQ=255)

                                                       gACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCaa                                     <  2:418058/93‑1 (MQ=255)

                                                         cATCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAgg                                   <  2:5021059/93‑1 (MQ=255)

                                                          aTCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAggg                                  >  2:3220946/1‑93 (MQ=255)

                                                          aTCAGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAggg                                  <  1:1805795/93‑1 (MQ=255)

                                                          aTCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAggg                                  >  2:1248634/1‑93 (MQ=255)

                                                             aGCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTg                               <  2:7358493/93‑1 (MQ=255)

                                                              gCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                              >  1:6045269/1‑93 (MQ=255)

                                                              gCCTGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                              <  2:3392432/93‑1 (MQ=255)

                                                              gCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                              <  1:1619452/93‑1 (MQ=255)

                                                                 tGGTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGc                           <  1:5326332/93‑1 (MQ=255)

                                                                  ggTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCa                          >  2:4966274/1‑93 (MQ=255)

                                                                   gTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCAc                         <  2:4848155/93‑1 (MQ=255)

                                                                   gTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCAc                         >  2:5327460/1‑93 (MQ=255)

                                                                   gTGCCAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCAc                         >  1:7468720/1‑93 (MQ=255)

                                                                       cAACCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                     >  1:6743206/1‑93 (MQ=255)

                                                                        aaCCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGATTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                    <  1:4086071/93‑1 (MQ=255)

                                                                        aaCCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                    >  1:6992464/1‑93 (MQ=255)

                                                                        aaCCTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                    <  1:6828003/93‑1 (MQ=255)

                                                                        aaCCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                    <  2:1451059/93‑1 (MQ=255)

                                                                          ccTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAAcc                  >  2:5390783/1‑93 (MQ=255)

                                                                          ccTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAAcc                  <  1:4944434/93‑1 (MQ=255)

                                                                           cTTTGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAAccc                 >  1:4036599/1‑93 (MQ=255)

                                                                            tttGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCa                >  1:3283081/1‑93 (MQ=255)

                                                                             ttGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCaa               <  1:1073378/93‑1 (MQ=255)

                                                                             ttGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCaa               >  2:5830207/1‑93 (MQ=255)

                                                                             ttGTGTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCaa               <  1:4553224/93‑1 (MQ=255)

                                                                              tgtgTTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAAt              <  1:4252887/93‑1 (MQ=255)

                                                                                tgtTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCg            >  2:1689194/1‑93 (MQ=255)

                                                                                 gtTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGt           <  2:6601112/93‑1 (MQ=255)

                                                                                 gtTAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGt           >  1:6121890/1‑93 (MQ=255)

                                                                                  ttAACGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt          <  2:623934/93‑1 (MQ=255)

                                                                                    aaCGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGGCTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTta        <  1:881086/93‑1 (MQ=255)

                                                                                     aCGCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTtat       <  1:2272730/93‑1 (MQ=255)

                                                                                       gCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat     <  2:5176063/93‑1 (MQ=255)

                                                                                       gCCGCTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat     >  2:4419427/1‑93 (MQ=255)

GGTGTTGTTCTCCCTTGCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTGCCAACCTTTGTGTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGAT  >  NC_000913/144151‑144333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`