BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,746,233	T→C	V360A (GTA→GCA)	b1668 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,746,233	0	T	C	82.8%	568.2 / 115.1	262	V360A (GTA→GCA)	b1668	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (26/19); new base C (108/109); total (134/128)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 264 total aligned reads displayed.

CAGAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGCGGCAATCC  >  NC_000913/1746143‑1746325

cAGAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCAc                                                                                            >  1:218824/1‑93 (MQ=255)

 aGAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGcaca                                                                                           <  1:4211909/93‑1 (MQ=255)

  gAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAg                                                                                          <  2:6731566/93‑1 (MQ=255)

  gAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAg                                                                                          <  1:6984987/93‑1 (MQ=255)

  gAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAg                                                                                          <  2:2589540/93‑1 (MQ=255)

    aaaaaaTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGa                                                                                        >  2:6048504/1‑93 (MQ=255)

    aaaaaaTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGa                                                                                        >  1:857666/1‑93 (MQ=255)

    aaaaaaTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGa                                                                                        <  2:2027334/93‑1 (MQ=255)

       aaaTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAAt                                                                                     >  2:6622081/1‑93 (MQ=255)

        aaTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAAtt                                                                                    <  1:7223196/93‑1 (MQ=255)

         aTCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTg                                                                                   >  1:7419879/1‑93 (MQ=255)

          tctcCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGt                                                                                  <  1:6087001/93‑1 (MQ=255)

          tctcCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGt                                                                                  >  2:4643941/1‑93 (MQ=255)

            tcCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTc                                                                                <  1:5782785/93‑1 (MQ=255)

            tcCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTc                                                                                >  2:6288709/1‑93 (MQ=255)

             ccAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTcc                                                                               >  1:4739201/1‑93 (MQ=255)

             ccAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTcc                                                                               >  2:5039474/1‑93 (MQ=255)

              cAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCca                                                                              >  1:2030256/1‑93 (MQ=255)

              cAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCca                                                                              >  2:5358949/1‑93 (MQ=255)

                ggAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCcaca                                                                            >  1:3822815/1‑93 (MQ=255)

                ggAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCcaca                                                                            >  1:5206395/1‑93 (MQ=255)

                 gAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACAt                                                                           <  1:6567643/93‑1 (MQ=255)

                  aaGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATc                                                                          <  2:5480917/93‑1 (MQ=255)

                   aGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCt                                                                         <  1:926258/93‑1 (MQ=255)

                    gTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCtg                                                                        >  2:2108161/1‑93 (MQ=255)

                    gTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCtg                                                                        >  1:7149283/1‑93 (MQ=255)

                        aaCGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCtgaatg                                                                    <  1:3648968/93‑1 (MQ=255)

                        aaCGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCtgaatg                                                                    >  1:5150889/1‑93 (MQ=255)

                          cGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCtgaatgaa                                                                  <  1:3222432/93‑1 (MQ=255)

                           gCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAac                                                                 >  1:3426062/1‑93 (MQ=255)

                           gCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAac                                                                 >  1:5361189/1‑93 (MQ=255)

                           gCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAac                                                                 >  2:2798020/1‑93 (MQ=255)

                           gCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAac                                                                 <  2:6885545/93‑1 (MQ=255)

                            cAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAaca                                                                <  1:4844879/93‑1 (MQ=255)

                            cAATAAACGAGAAAAACATACTGTTCCCAGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAaca                                                                >  1:116605/1‑93 (MQ=255)

                             aaTAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAacac                                                               <  1:6700416/93‑1 (MQ=255)

                               tAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGa                                                             >  2:4323531/1‑93 (MQ=255)

                               tAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGa                                                             <  1:4270933/93‑1 (MQ=255)

                               tAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGa                                                             <  2:3804348/93‑1 (MQ=255)

                                aaaCGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGAc                                                            <  2:2945767/93‑1 (MQ=255)

                                 aaCGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGAcc                                                           >  1:4037473/1‑93 (MQ=255)

                                  aCGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCa                                                          <  1:1966211/93‑1 (MQ=255)

                                  aCGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCa                                                          >  1:637438/1‑93 (MQ=255)

                                    gagaAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATa                                                        >  1:7190466/1‑93 (MQ=255)

                                    gagaAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATa                                                        <  1:2885403/93‑1 (MQ=255)

                                     agaAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATaa                                                       >  1:4378933/1‑93 (MQ=255)

                                      gaAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATaaa                                                      <  2:4230325/93‑1 (MQ=255)

                                       aaaaaCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAAc                                                     <  1:1301494/93‑1 (MQ=255)

                                          aaCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGt                                                  <  2:2687895/93‑1 (MQ=255)

                                          aaCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGt                                                  >  1:244689/1‑93 (MQ=255)

                                          aaCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGt                                                  <  1:4559631/93‑1 (MQ=255)

                                           aCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGtt                                                 >  2:5219929/1‑93 (MQ=255)

                                           aCATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGtt                                                 <  2:3056038/93‑1 (MQ=255)

                                            cATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTc                                                <  2:1472076/93‑1 (MQ=255)

                                            cATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTc                                                >  2:5800396/1‑93 (MQ=255)

                                            cATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTc                                                <  2:7035164/93‑1 (MQ=255)

                                            cATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTc                                                <  1:4316478/93‑1 (MQ=255)

                                              tACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAg                                              <  2:4941917/93‑1 (MQ=255)

                                              tACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAg                                              <  1:5558157/93‑1 (MQ=255)

                                                cTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTa                                            <  2:1103176/93‑1 (MQ=255)

                                                 tGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTaa                                           >  2:5644986/1‑93 (MQ=255)

                                                 tGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTaa                                           >  2:6307622/1‑93 (MQ=255)

                                                 tGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTaa                                           <  1:6202716/93‑1 (MQ=255)

                                                  gTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTaaa                                          <  2:3065185/93‑1 (MQ=255)

                                                    tCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAgg                                        <  2:2881662/93‑1 (MQ=255)

                                                    tCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAgg                                        >  1:35491/1‑93 (MQ=255)

                                                      ccTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGcc                                      >  1:308477/1‑93 (MQ=255)

                                                      ccTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGcc                                      <  2:6300516/93‑1 (MQ=255)

                                                      ccTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGcc                                      >  1:161946/1‑93 (MQ=255)

                                                      ccTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGcc                                      >  1:7161601/1‑93 (MQ=255)

                                                       cTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCt                                     <  2:6472944/93‑1 (MQ=255)

                                                        tGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCtt                                    <  1:2697394/93‑1 (MQ=255)

                                                        tGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCtt                                    <  2:651332/93‑1 (MQ=255)

                                                          gCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGc                                  >  2:917454/1‑93 (MQ=255)

                                                          gCGTTATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGc                                  <  2:3888131/93‑1 (MQ=255)

                                                             ttATGTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCg                               >  1:4193674/1‑93 (MQ=255)

                                                                tGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGt                            >  1:2356598/1‑93 (MQ=255)

                                                                tGTCATTCTGCGCCTGCATGAAGCCGCACAGGATATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGt                            >  2:2638199/1‑93 (MQ=255)

                                                                 gTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGtt                           >  1:5452679/1‑93 (MQ=255)

                                                                 gTCATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGtt                           >  2:4851636/1‑93 (MQ=255)

                                                                  tCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGttt                          >  2:6109312/1‑93 (MQ=255)

                                                                   cATTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGtttt                         <  2:3487067/93‑1 (MQ=255)

                                                                    aTTCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTc                        >  2:2175928/1‑93 (MQ=255)

                                                                     ttCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCa                       >  2:1573618/1‑93 (MQ=255)

                                                                     ttCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCa                       >  2:1493975/1‑93 (MQ=255)

                                                                     ttCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCa                       >  2:4134231/1‑93 (MQ=255)

                                                                     ttCTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCa                       >  2:4979762/1‑93 (MQ=255)

                                                                      tCTGCGCCTGCATGAAGCTGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCAt                      >  2:7071024/1‑93 (MQ=255)

                                                                       cTGCGCCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATc                     <  2:2364059/93‑1 (MQ=255)

                                                                         gcgcCTGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGa                   <  2:7334021/93‑1 (MQ=255)

                                                                            ccTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATaa                <  1:1418787/93‑1 (MQ=255)

                                                                              tGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAAtt              >  2:5701212/1‑93 (MQ=255)

                                                                              tGCATGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAAtt              <  2:3655621/93‑1 (MQ=255)

                                                                                 aTGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATg           >  2:1978452/1‑93 (MQ=255)

                                                                                 aTGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATg           <  2:3451464/93‑1 (MQ=255)

                                                                                 aTGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATg           >  2:812967/1‑93 (MQ=255)

                                                                                  tGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGc          >  2:6769980/1‑93 (MQ=255)

                                                                                  tGAAGCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGc          >  1:1036654/1‑93 (MQ=255)

                                                                                    aaGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGCgg        <  2:3406819/93‑1 (MQ=255)

                                                                                      gCCGCACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGCGGCa      <  2:747338/93‑1 (MQ=255)

CAGAAAAAAATCTCCAGGAAGTCGAACGCAATAAACGAGAAAAACATACTGTTCCCTGGCGTTATGTCATTCTGCGCCTGCATGAAGCCGTACAGGAAATTGTTCCACATCTGAATGAACACGACCATAAACGGTTCAGTAAAGGCCTTGCCCGGGTTTTCATCGATAATTATGCGGCAATCC  >  NC_000913/1746143‑1746325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`