BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,766,232	T→C	T160A (ACC→GCC)	ydiJ ←	putative oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,766,232	0	T	C	83.1%	528.5 / 93.4	237	T160A (ACC→GCC)	ydiJ	putative oxidase
Reads supporting (aligned to +/- strand): ref base T (21/19); new base C (101/96); total (122/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01

Only 100 of 237 total aligned reads displayed.

GAATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGA  >  NC_000913/1766142‑1766323

gaATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCAt                                                                                           >  2:3654516/1‑93 (MQ=255)

 aaTATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCAAAGACCAGCGATCCCTGACCGGATGCATCGGCAtt                                                                                          >  1:6611284/1‑93 (MQ=255)

  atatCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTg                                                                                         >  2:3973249/1‑93 (MQ=255)

   tatCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGa                                                                                        >  1:6785831/1‑93 (MQ=255)

   tatCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCACTGACCGGATGCATCGGCATTGa                                                                                        >  1:1064665/1‑93 (MQ=255)

     tCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGatc                                                                                      <  2:6292956/93‑1 (MQ=255)

       gCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGatcat                                                                                    <  2:2104987/93‑1 (MQ=255)

       gCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGatcat                                                                                    >  1:5219007/1‑93 (MQ=255)

        ccccccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGatcatc                                                                                   <  2:1177631/93‑1 (MQ=255)

          ccccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATccc                                                                                 >  1:1248379/1‑93 (MQ=255)

           cccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCa                                                                                >  1:6918360/1‑93 (MQ=255)

            ccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCAc                                                                               <  2:3023697/93‑1 (MQ=255)

            ccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCAc                                                                               <  2:3551839/93‑1 (MQ=255)

             caacaaCACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCAcc                                                                              >  1:2760882/1‑93 (MQ=255)

               acaacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGa                                                                            >  1:1665488/1‑93 (MQ=255)

                caacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAg                                                                           >  1:1671598/1‑93 (MQ=255)

                caacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAg                                                                           <  1:3417354/93‑1 (MQ=255)

                caacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAg                                                                           >  2:584871/1‑93 (MQ=255)

                   cacCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGt                                                                        <  2:151479/93‑1 (MQ=255)

                       gcgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGcc                                                                    <  2:460408/93‑1 (MQ=255)

                        cgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGccc                                                                   <  1:435490/93‑1 (MQ=255)

                        cgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGccc                                                                   <  2:1928564/93‑1 (MQ=255)

                         gcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCg                                                                  <  2:3308704/93‑1 (MQ=255)

                         gcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCg                                                                  <  1:5822025/93‑1 (MQ=255)

                            tACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGtt                                                               >  1:2467193/1‑93 (MQ=255)

                            tACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGtt                                                               >  1:1990493/1‑93 (MQ=255)

                             aCGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTg                                                              <  1:1923286/93‑1 (MQ=255)

                               gCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCt                                                            >  2:2657563/1‑93 (MQ=255)

                                 cAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTgg                                                          <  1:4685999/93‑1 (MQ=255)

                                 cAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTgg                                                          >  1:6640567/1‑93 (MQ=255)

                                 cAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTgg                                                          >  2:47425/1‑93 (MQ=255)

                                 cAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTgg                                                          >  2:198644/1‑93 (MQ=255)

                                  aaGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGt                                                         >  1:163299/1‑93 (MQ=255)

                                  aaGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGt                                                         >  1:2346698/1‑93 (MQ=255)

                                   aGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTc                                                        <  2:6780227/93‑1 (MQ=255)

                                    gTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCg                                                       <  2:5522830/93‑1 (MQ=255)

                                     tACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGa                                                      >  2:7515137/1‑93 (MQ=255)

                                       cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGaaa                                                    <  2:5962465/93‑1 (MQ=255)

                                       cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGaaa                                                    <  1:5379150/93‑1 (MQ=255)

                                          gATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGtt                                                 >  2:1045729/1‑93 (MQ=255)

                                            tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc                                               >  2:3805192/1‑93 (MQ=255)

                                            tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc                                               >  2:1259508/1‑93 (MQ=255)

                                            tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc                                               >  1:4241412/1‑93 (MQ=255)

                                            tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTcc                                               >  2:1901574/1‑93 (MQ=255)

                                             cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCg                                              >  2:2969722/1‑93 (MQ=255)

                                             cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCg                                              <  2:7004258/93‑1 (MQ=255)

                                              tGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCgg                                             >  2:3203204/1‑93 (MQ=255)

                                              tGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCgg                                             >  1:6175557/1‑93 (MQ=255)

                                               gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGgc                                            <  2:719728/93‑1 (MQ=255)

                                               gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGgc                                            <  2:6521126/93‑1 (MQ=255)

                                               gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGgc                                            <  1:1143176/93‑1 (MQ=255)

                                               gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGgc                                            <  1:6274431/93‑1 (MQ=255)

                                                  gTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGCGCGTTGCCCGGTTGCTGGTCGAAAGATCCGGCGCa                                         >  2:2277119/1‑93 (MQ=255)

                                                   ttttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCaa                                        >  2:5903509/1‑93 (MQ=255)

                                                     ttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCaaaa                                      <  1:578771/93‑1 (MQ=255)

                                                     ttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCaaaa                                      <  2:2368562/93‑1 (MQ=255)

                                                       gCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCaaaaaa                                    <  1:440712/93‑1 (MQ=255)

                                                       gCCATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCaaaaaa                                    >  2:3376686/1‑93 (MQ=255)

                                                        ccATAGACCAGCGATCCCTGACCGGATGTATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAg                                   <  1:567943/93‑1 (MQ=255)

                                                        ccATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAg                                   >  1:3336819/1‑93 (MQ=255)

                                                        ccATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAg                                   >  1:2942653/1‑93 (MQ=255)

                                                         cATAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGt                                  >  1:6539232/1‑93 (MQ=255)

                                                          aTAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTa                                 <  1:7338961/93‑1 (MQ=255)

                                                          aTAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTa                                 >  2:6783845/1‑93 (MQ=255)

                                                           tAGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAg                                >  2:5011868/1‑93 (MQ=255)

                                                            aGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGc                               <  1:6436445/93‑1 (MQ=255)

                                                            aGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGc                               <  1:6409808/93‑1 (MQ=255)

                                                            aGACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGc                               <  2:4828601/93‑1 (MQ=255)

                                                             gACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGcc                              >  1:6110766/1‑93 (MQ=255)

                                                             gACCAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGcc                              <  2:6937924/93‑1 (MQ=255)

                                                               ccAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGa                            >  2:341990/1‑93 (MQ=255)

                                                                cAGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGaa                           >  1:3218288/1‑93 (MQ=255)

                                                                 aGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAAc                          <  2:6757818/93‑1 (MQ=255)

                                                                 aGCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAAc                          >  2:4923026/1‑93 (MQ=255)

                                                                  gCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACg                         >  2:2467684/1‑93 (MQ=255)

                                                                  gCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACg                         >  1:3711888/1‑93 (MQ=255)

                                                                  gCGATCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACg                         >  1:6749438/1‑93 (MQ=255)

                                                                    gATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGt                       <  2:2220767/93‑1 (MQ=255)

                                                                     aTCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGtt                      <  1:418789/93‑1 (MQ=255)

                                                                     aTCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGtt                      <  1:2036093/93‑1 (MQ=255)

                                                                      tCCCTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGttt                     >  1:3958622/1‑93 (MQ=255)

                                                                       cccTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTc                    >  1:7158535/1‑93 (MQ=255)

                                                                        ccTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCa                   <  1:4900947/93‑1 (MQ=255)

                                                                        ccTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCa                   >  2:281614/1‑93 (MQ=255)

                                                                         cTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAg                  >  1:3106000/1‑93 (MQ=255)

                                                                         cTGACCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAg                  >  1:5032761/1‑93 (MQ=255)

                                                                          tGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAgg                 >  2:5576079/1‑93 (MQ=255)

                                                                            aCCGGATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTa               <  2:4429329/93‑1 (MQ=255)

                                                                               ggATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTg            >  2:5262472/1‑93 (MQ=255)

                                                                                gATGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGa           <  1:3365656/93‑1 (MQ=255)

                                                                                 aTGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGAt          <  2:4276758/93‑1 (MQ=255)

                                                                                  tGCATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGAtt         >  2:1727416/1‑93 (MQ=255)

                                                                                    cATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGa       <  2:5745093/93‑1 (MQ=255)

                                                                                    cATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGa       <  2:3752709/93‑1 (MQ=255)

                                                                                    cATCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGa       <  2:765694/93‑1 (MQ=255)

                                                                                     aTCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAg      >  2:1011912/1‑93 (MQ=255)

                                                                                     aTCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAg      >  2:2743856/1‑93 (MQ=255)

                                                                                     aTCGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAg      <  2:2636406/93‑1 (MQ=255)

                                                                                       cGGCATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGtt    <  2:2321032/93‑1 (MQ=255)

                                                                                         gTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGa  <  1:4944447/93‑1 (MQ=255)

GAATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGA  >  NC_000913/1766142‑1766323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`