BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,301	T→C	V143A (GTG→GCG)	yeaD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,301	0	T	C	86.9%	584.5 / 69.8	237	V143A (GTG→GCG)	yeaD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (16/15); new base C (105/101); total (121/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 240 total aligned reads displayed.

TGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCG  >  NC_000913/1862209‑1862390

tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                                                           <  2:3964987/93‑1 (MQ=255)

  aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCgg                                                                                         <  2:3361509/93‑1 (MQ=255)

  aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCgg                                                                                         >  2:5865821/1‑93 (MQ=255)

   tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCggg                                                                                        >  1:3555244/1‑93 (MQ=255)

   tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCggg                                                                                        >  1:967486/1‑93 (MQ=255)

        tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaaa                                                                                   <  2:2230003/93‑1 (MQ=255)

        tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaaa                                                                                   >  2:2932670/1‑93 (MQ=255)

        tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaaa                                                                                   <  1:1303597/93‑1 (MQ=255)

        tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaaa                                                                                   >  1:5800521/1‑93 (MQ=255)

         aGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAAc                                                                                  >  1:6603995/1‑93 (MQ=255)

          gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                 >  2:2588669/1‑93 (MQ=255)

           cgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACtt                                                                                >  1:2517045/1‑93 (MQ=255)

            gcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTtg                                                                               >  2:6582914/1‑93 (MQ=255)

             cTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTtgt                                                                              >  2:4634062/1‑93 (MQ=255)

             cTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTtgt                                                                              >  2:5220041/1‑93 (MQ=255)

               gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGa                                                                            <  1:7028191/93‑1 (MQ=255)

               gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGa                                                                            >  1:578424/1‑93 (MQ=255)

               gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGa                                                                            >  1:2276832/1‑93 (MQ=255)

               gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGa                                                                            >  1:2242274/1‑93 (MQ=255)

                aCTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGaa                                                                           <  2:3711678/93‑1 (MQ=255)

                   tttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATc                                                                        <  2:1351813/93‑1 (MQ=255)

                   tttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATc                                                                        >  1:6398306/1‑93 (MQ=255)

                   tttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATc                                                                        <  1:6260151/93‑1 (MQ=255)

                    ttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCg                                                                       >  2:4683354/1‑93 (MQ=255)

                      gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGAt                                                                     <  1:88862/93‑1 (MQ=255)

                      gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGAt                                                                     <  2:2525538/93‑1 (MQ=255)

                      gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGAt                                                                     <  1:1065578/93‑1 (MQ=255)

                        aTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                                                                   >  1:126675/1‑93 (MQ=255)

                        aTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCt                                                                   <  1:4825976/93‑1 (MQ=255)

                         ttGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCtt                                                                  <  1:6478147/93‑1 (MQ=255)

                         ttGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCtt                                                                  <  1:5947434/93‑1 (MQ=255)

                              gCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATc                                                             <  1:5393035/93‑1 (MQ=255)

                               cAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATca                                                            >  2:1465075/1‑93 (MQ=255)

                                aaaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcac                                                           >  2:2100798/1‑93 (MQ=255)

                                aaaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcac                                                           >  2:5481907/1‑93 (MQ=255)

                                 aaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcaca                                                          <  2:5944374/93‑1 (MQ=255)

                                 aaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcaca                                                          <  1:7388144/93‑1 (MQ=255)

                                   gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                        >  2:126692/1‑93 (MQ=255)

                                   gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                        <  2:4371325/93‑1 (MQ=255)

                                   gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                        <  2:3765701/93‑1 (MQ=255)

                                     gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      <  1:1982108/93‑1 (MQ=255)

                                     gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      <  1:4551735/93‑1 (MQ=255)

                                     gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      >  1:476025/1‑93 (MQ=255)

                                     gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      >  1:1915741/1‑93 (MQ=255)

                                      aaGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCg                                                     <  1:3409445/93‑1 (MQ=255)

                                       agagACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGa                                                    >  1:3838500/1‑93 (MQ=255)

                                       agagACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGa                                                    >  2:7075184/1‑93 (MQ=255)

                                        gagaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGaa                                                   >  2:6863383/1‑93 (MQ=255)

                                         agaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAt                                                  >  2:4085910/1‑93 (MQ=255)

                                         agaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAt                                                  >  1:440963/1‑93 (MQ=255)

                                          gaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt                                                 <  1:5023580/93‑1 (MQ=255)

                                           aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAttt                                                >  1:7087983/1‑93 (MQ=255)

                                           aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAttt                                                >  2:182332/1‑93 (MQ=255)

                                            cGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTg                                               >  2:4768464/1‑93 (MQ=255)

                                             gAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGa                                              <  2:6567329/93‑1 (MQ=255)

                                                 aaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc                                          <  1:6744322/93‑1 (MQ=255)

                                                 aaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc                                          >  1:1387441/1‑93 (MQ=255)

                                                  aaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacca                                         <  1:6129158/93‑1 (MQ=255)

                                                   aTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAaccac                                        >  1:360165/1‑93 (MQ=255)

                                                    ttCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAaccacc                                       >  2:5506047/1‑93 (MQ=255)

                                                    ttCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAaccacc                                       <  1:3226338/93‑1 (MQ=255)

                                                      cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  1:434035/93‑1 (MQ=255)

                                                      cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  1:4935511/93‑1 (MQ=255)

                                                      cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  2:4847161/93‑1 (MQ=255)

                                                       tGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct                                    <  2:508976/93‑1 (MQ=255)

                                                       tGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct                                    >  1:986359/1‑93 (MQ=255)

                                                        ggCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                   >  1:2535637/1‑93 (MQ=255)

                                                            gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                               >  1:5480633/1‑93 (MQ=255)

                                                            gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                               >  2:6281811/1‑93 (MQ=255)

                                                            gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                               >  1:2024670/1‑93 (MQ=255)

                                                             cACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTg                              >  2:3525022/1‑93 (MQ=255)

                                                              aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                             >  2:7391637/1‑93 (MQ=255)

                                                              aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                             >  2:1506105/1‑93 (MQ=255)

                                                               cGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCa                            <  2:901956/93‑1 (MQ=255)

                                                               cGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCa                            >  2:6132323/1‑93 (MQ=255)

                                                               cGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCa                            <  2:452367/93‑1 (MQ=255)

                                                                gACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTCCAt                           <  1:6970757/93‑1 (MQ=255)

                                                                 aCTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          >  1:6698231/1‑93 (MQ=255)

                                                                   tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  1:3982685/1‑93 (MQ=255)

                                                                   tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  2:5394483/1‑93 (MQ=255)

                                                                      aCGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTAc                     <  2:1960462/93‑1 (MQ=255)

                                                                      aCGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTAc                     <  2:2342526/93‑1 (MQ=255)

                                                                      aCGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTAc                     >  2:7028458/1‑93 (MQ=255)

                                                                       cGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACt                    <  1:1686912/93‑1 (MQ=255)

                                                                       cGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACt                    >  2:1165074/1‑93 (MQ=255)

                                                                        gCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACtt                   <  2:6224602/93‑1 (MQ=255)

                                                                          tGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTa                 >  1:2424570/1‑93 (MQ=255)

                                                                           gTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTaa                >  1:5163563/1‑93 (MQ=255)

                                                                             tAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACg              <  1:1845843/93‑1 (MQ=255)

                                                                              aGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGt             <  1:4456607/93‑1 (MQ=255)

                                                                              aGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGt             <  2:2689189/93‑1 (MQ=255)

                                                                               gcgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTg            <  2:7521395/93‑1 (MQ=255)

                                                                               gcgcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTg            >  1:4220619/1‑93 (MQ=255)

                                                                                cgcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTgg           <  2:165979/93‑1 (MQ=255)

                                                                                 gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTggg          >  1:1454819/1‑93 (MQ=255)

                                                                                 gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTggg          >  2:1715989/1‑93 (MQ=255)

                                                                                 gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTggg          <  2:879823/93‑1 (MQ=255)

                                                                                  cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt         <  1:2988230/93‑1 (MQ=255)

                                                                                    tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa       <  2:6522855/93‑1 (MQ=255)

                                                                                    tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa       >  1:3868708/1‑93 (MQ=255)

TGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCG  >  NC_000913/1862209‑1862390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`