BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,764	T→C	T245A (ACC→GCC)	yeaF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,764	0	T	C	83.6%	512.4 / 96.0	233	T245A (ACC→GCC)	yeaF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (16/22); new base C (100/94); total (117/116)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

Only 100 of 234 total aligned reads displayed.

AGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAC  >  NC_000913/1863672‑1863853

  gTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                                                                         <  1:6658473/93‑1 (MQ=255)

  gTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGa                                                                                         >  2:5298701/1‑93 (MQ=255)

     ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATcc                                                                                      <  2:478233/93‑1 (MQ=255)

      tGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATccc                                                                                     <  1:6543938/93‑1 (MQ=255)

      tGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATccc                                                                                     <  1:2068455/93‑1 (MQ=255)

       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCg                                                                                    <  1:6277746/93‑1 (MQ=255)

       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCg                                                                                    <  1:3934705/93‑1 (MQ=255)

       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCg                                                                                    >  2:4554134/1‑93 (MQ=255)

        caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCgg                                                                                   <  2:846377/93‑1 (MQ=255)

        caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCgg                                                                                   >  1:3579210/1‑93 (MQ=255)

         accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGt                                                                                  <  2:5821056/93‑1 (MQ=255)

         accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGt                                                                                  >  1:1681980/1‑93 (MQ=255)

         accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGt                                                                                  >  2:4634451/1‑93 (MQ=255)

           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAg                                                                                >  1:4546758/1‑93 (MQ=255)

             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGaa                                                                              <  2:7019197/93‑1 (MQ=255)

              cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGaaa                                                                             >  1:5057458/1‑93 (MQ=255)

               gTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAAt                                                                            >  1:1726120/1‑93 (MQ=255)

               gTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAAt                                                                            >  2:6141549/1‑93 (MQ=255)

                tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATc                                                                           <  1:2476270/93‑1 (MQ=255)

                tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATc                                                                           <  1:5339111/93‑1 (MQ=255)

                tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATc                                                                           <  1:5966665/93‑1 (MQ=255)

                 aaTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCa                                                                          <  2:1840420/93‑1 (MQ=255)

                 aaTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAAAAGCATTATAAAAAATTTTATGGGATACCCGGAGaaaaaa                                                                          >  1:4720612/1‑90 (MQ=255)

                  aTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAg                                                                         <  1:5738824/93‑1 (MQ=255)

                   tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAgg                                                                        <  2:6828013/93‑1 (MQ=255)

                    gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGc                                                                       >  1:3112355/1‑93 (MQ=255)

                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                                                                      <  1:5524503/93‑1 (MQ=255)

                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGcc                                                                      <  1:4918539/93‑1 (MQ=255)

                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGcc                                                                      >  1:6611086/1‑93 (MQ=255)

                        aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGt                                                                   <  1:120434/93‑1 (MQ=255)

                           aGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCa                                                                <  1:763803/93‑1 (MQ=255)

                           aGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCa                                                                >  1:7319219/1‑93 (MQ=255)

                            gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAg                                                               >  1:1953227/1‑93 (MQ=255)

                               cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                            <  1:7071278/93‑1 (MQ=255)

                                gTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                           >  2:327704/1‑93 (MQ=255)

                                  gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTa                                                         <  2:1638415/93‑1 (MQ=255)

                                   aCGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt                                                        >  1:2572664/1‑93 (MQ=255)

                                    cGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATc                                                       <  1:2956160/93‑1 (MQ=255)

                                      cccTGTTTTTATGCACATAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                     >  2:4493844/1‑93 (MQ=255)

                                      cccTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                     <  2:3341943/93‑1 (MQ=255)

                                      cccTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                     >  1:4899395/1‑93 (MQ=255)

                                      cccTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                     >  1:5188647/1‑93 (MQ=255)

                                      cccTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                     >  2:3031574/1‑93 (MQ=255)

                                        cTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc                                                   >  1:6880872/1‑93 (MQ=255)

                                         tGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacca                                                  >  1:3296392/1‑93 (MQ=255)

                                         tGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacca                                                  >  2:2932350/1‑93 (MQ=255)

                                          gTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCAt                                                 >  2:3148146/1‑93 (MQ=255)

                                           tttttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                >  1:6982176/1‑93 (MQ=255)

                                           tttttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                <  2:2188619/93‑1 (MQ=255)

                                            ttttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCg                                               <  1:4148742/93‑1 (MQ=255)

                                            ttttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCg                                               <  2:4803856/93‑1 (MQ=255)

                                             tttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCTACCATCgg                                              >  2:3929778/1‑93 (MQ=255)

                                             tttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                              <  1:2234437/93‑1 (MQ=255)

                                             tttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                              >  2:2096123/1‑93 (MQ=255)

                                              ttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCggg                                             <  2:248212/93‑1 (MQ=255)

                                               tATGCACAAAATGCCCTGGAAAGATGCATAATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGc                                            <  1:2152002/93‑1 (MQ=255)

                                                   cacaAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTc                                        >  1:4605713/1‑93 (MQ=255)

                                                     caAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAg                                      >  2:2867172/1‑93 (MQ=255)

                                                      aaaaTGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                     >  2:2051429/1‑93 (MQ=255)

                                                        aaTGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                   >  1:7466223/1‑93 (MQ=255)

                                                         aTGCCCTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAAc                                  <  2:2360418/93‑1 (MQ=255)

                                                              cTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcat                             >  1:1475312/1‑93 (MQ=255)

                                                              cTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcat                             <  1:1153509/93‑1 (MQ=255)

                                                              cTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcat                             >  2:7251390/1‑93 (MQ=255)

                                                              cTGGAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcat                             >  2:76847/1‑93 (MQ=255)

                                                               tGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatc                            <  2:3486327/93‑1 (MQ=255)

                                                                ggAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatca                           >  2:4042486/1‑93 (MQ=255)

                                                                ggAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatca                           <  2:7380604/93‑1 (MQ=255)

                                                                 gAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcag                          <  1:953609/93‑1 (MQ=255)

                                                                 gAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcag                          >  2:1849934/1‑93 (MQ=255)

                                                                 gAAAGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcag                          >  2:5959257/1‑93 (MQ=255)

                                                                  aaaGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcaga                         <  1:563689/93‑1 (MQ=255)

                                                                  aaaGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcaga                         <  1:7195954/93‑1 (MQ=255)

                                                                   aaGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagac                        >  2:4204387/1‑93 (MQ=255)

                                                                    aGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagaca                       <  1:6787843/93‑1 (MQ=255)

                                                                    aGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagaca                       >  2:6693174/1‑93 (MQ=255)

                                                                    aGATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagaca                       >  1:1412226/1‑93 (MQ=255)

                                                                     gATGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacag                      >  1:6459809/1‑93 (MQ=255)

                                                                      aTGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                     <  2:718109/93‑1 (MQ=255)

                                                                      aTGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                     <  1:1648312/93‑1 (MQ=255)

                                                                      aTGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                     <  2:5218376/93‑1 (MQ=255)

                                                                      aTGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                     <  2:5681905/93‑1 (MQ=255)

                                                                       tGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCagacagac                    >  2:6414543/1‑93 (MQ=255)

                                                                       tGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCagacagac                    >  1:1527154/1‑93 (MQ=255)

                                                                       tGCATTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCagacagac                    >  2:5063199/1‑93 (MQ=255)

                                                                          aTTATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACggg                 <  2:2742085/93‑1 (MQ=255)

                                                                           ttATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAATTTCATCAGACAGACGGgt                <  2:1221971/93‑1 (MQ=255)

                                                                           ttATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                <  1:2617258/93‑1 (MQ=255)

                                                                           ttATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                <  2:6572673/93‑1 (MQ=255)

                                                                           ttATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                <  1:2287461/93‑1 (MQ=255)

                                                                           ttATCAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                <  1:3624515/93‑1 (MQ=255)

                                                                              tCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa             >  1:7113031/1‑93 (MQ=255)

                                                                              ccAGAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa             <  1:1037891/92‑1 (MQ=255)

                                                                                 gAATTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCg          <  2:108041/93‑1 (MQ=255)

                                                                                  aaTTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGt         >  2:4601467/1‑93 (MQ=255)

                                                                                   aTTTGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg        >  1:2263313/1‑93 (MQ=255)

                                                                                    tttGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc       >  1:4391283/1‑93 (MQ=255)

                                                                                    tttGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc       <  2:5352924/93‑1 (MQ=255)

                                                                                    tttGTAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGc       >  2:352071/1‑93 (MQ=255)

                                                                                        tAGGCGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTa   >  1:3487859/1‑93 (MQ=255)

AGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAC  >  NC_000913/1863672‑1863853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`