BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,741	T→C	V286A (GTA→GCA)	yeaJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,741	0	T	C	84.8%	610.3 / 89.7	257	V286A (GTA→GCA)	yeaJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (13/26); new base C (91/127); total (104/153)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 268 total aligned reads displayed.

ATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTC  >  NC_000913/1870650‑1870830

 ttGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGtat                                                                                         >  2:2616108/1‑93 (MQ=255)

 ttGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGcat                                                                                         >  1:5007228/1‑93 (MQ=255)

 ttGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGcat                                                                                         >  2:1422524/1‑93 (MQ=255)

  tGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGcata                                                                                        <  2:662227/93‑1 (MQ=255)

   gAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCatat                                                                                       <  2:2706892/93‑1 (MQ=255)

   gAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCatat                                                                                       <  1:3291131/93‑1 (MQ=255)

    aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATg                                                                                      <  1:7061457/93‑1 (MQ=255)

    aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATg                                                                                      <  2:4317213/93‑1 (MQ=255)

    aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATg                                                                                      >  1:3734798/1‑93 (MQ=255)

      aaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTc                                                                                    <  1:1143035/93‑1 (MQ=255)

       aGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCg                                                                                   <  1:6601304/93‑1 (MQ=255)

       aGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCg                                                                                   >  2:4646050/1‑93 (MQ=255)

        ggTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGc                                                                                  >  1:5953195/1‑93 (MQ=255)

        ggTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAAGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGc                                                                                  >  1:6226145/1‑93 (MQ=255)

         gTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCa                                                                                 <  2:1968580/93‑1 (MQ=255)

          tATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAg                                                                                <  2:4353634/93‑1 (MQ=255)

          tATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAg                                                                                <  1:3951754/93‑1 (MQ=255)

           aTTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAgg                                                                               <  1:2495558/93‑1 (MQ=255)

             ttACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGc                                                                             <  1:6289272/93‑1 (MQ=255)

              tACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCa                                                                            <  1:266290/93‑1 (MQ=255)

                   ggCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaa                                                                       >  1:4524164/1‑93 (MQ=255)

                    gCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaa                                                                      <  2:5763660/93‑1 (MQ=255)

                     cGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTaaaa                                                                     <  2:4989701/93‑1 (MQ=255)

                     cGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaaa                                                                     >  1:5519210/1‑93 (MQ=255)

                     cGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaaa                                                                     <  2:7154977/93‑1 (MQ=255)

                      gTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAg                                                                    >  1:1507811/1‑93 (MQ=255)

                       tACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAgg                                                                   >  2:5304915/1‑93 (MQ=255)

                       tACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAgg                                                                   >  2:2069548/1‑93 (MQ=255)

                       tACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAgg                                                                   <  2:6073219/93‑1 (MQ=255)

                         ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGa                                                                 <  2:636319/93‑1 (MQ=255)

                         ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGa                                                                 >  1:4028489/1‑93 (MQ=255)

                         ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGa                                                                 <  1:2014330/93‑1 (MQ=255)

                          cGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGAt                                                                >  1:486860/1‑93 (MQ=255)

                          cGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGAt                                                                <  1:5356032/93‑1 (MQ=255)

                          cGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGAt                                                                <  1:3098797/93‑1 (MQ=255)

                           gTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGAtt                                                               >  1:4696299/1‑93 (MQ=255)

                           gTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGAtt                                                               <  2:6770698/93‑1 (MQ=255)

                           gTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGAtt                                                               >  1:791081/1‑93 (MQ=255)

                            tATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATtg                                                              >  2:1361606/1‑93 (MQ=255)

                            tATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATtg                                                              >  1:1647532/1‑93 (MQ=255)

                             aTTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATtgt                                                             <  2:3346115/93‑1 (MQ=255)

                                gTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGtgct                                                          >  1:5416981/1‑93 (MQ=255)

                                gTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGtgct                                                          >  1:5205146/1‑93 (MQ=255)

                                 tCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGtgctg                                                         >  2:5451180/1‑93 (MQ=255)

                                   gAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTgctgct                                                       >  1:6987968/1‑93 (MQ=255)

                                      cATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGa                                                    <  2:7243689/93‑1 (MQ=255)

                                      cATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGa                                                    >  1:5147726/1‑93 (MQ=255)

                                       aTTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGat                                                   <  1:7306185/93‑1 (MQ=255)

                                         ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGatat                                                 <  2:4603671/93‑1 (MQ=255)

                                         ttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGatat                                                 <  2:1545128/93‑1 (MQ=255)

                                          tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATAtt                                                <  1:2383322/93‑1 (MQ=255)

                                           aCCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTa                                               >  1:5093933/1‑93 (MQ=255)

                                            ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              <  1:7228052/93‑1 (MQ=255)

                                            ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              >  2:3524983/1‑93 (MQ=255)

                                            ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              <  2:6674674/93‑1 (MQ=255)

                                            ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              >  1:1569001/1‑93 (MQ=255)

                                              aGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACa                                            >  1:1897717/1‑93 (MQ=255)

                                               ggATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaa                                           <  2:3760580/93‑1 (MQ=255)

                                               ggATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaa                                           <  2:7363068/93‑1 (MQ=255)

                                                gATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaa                                          <  1:6740097/93‑1 (MQ=255)

                                                  tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaaaa                                        <  2:7205725/93‑1 (MQ=255)

                                                  tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaaaa                                        <  2:3452325/93‑1 (MQ=255)

                                                     gCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACaa                                     <  1:1771940/93‑1 (MQ=255)

                                                     gCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACaa                                     <  2:3689896/93‑1 (MQ=255)

                                                       gTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATc                                   <  2:6884086/93‑1 (MQ=255)

                                                       gTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATc                                   <  2:1047377/93‑1 (MQ=255)

                                                       gTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATc                                   >  1:2586143/1‑93 (MQ=255)

                                                        tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  1:2722013/93‑1 (MQ=255)

                                                        tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  1:3001953/93‑1 (MQ=255)

                                                         aCTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTg                                 <  1:5747069/93‑1 (MQ=255)

                                                           tAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCg                               >  1:3695190/1‑93 (MQ=255)

                                                             aaCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGa                             <  2:5606254/93‑1 (MQ=255)

                                                               cAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAat                           >  2:4846566/1‑93 (MQ=255)

                                                                 gAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAatat                         >  1:6426334/1‑93 (MQ=255)

                                                                  aaCGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATc                        >  1:2879116/1‑93 (MQ=255)

                                                                  aaCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATc                        <  1:1083088/93‑1 (MQ=255)

                                                                   aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       <  1:5623935/93‑1 (MQ=255)

                                                                    cGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtt                      <  2:3314893/93‑1 (MQ=255)

                                                                     gTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCttt                     >  1:2805910/1‑93 (MQ=255)

                                                                      tgatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                    <  1:5796135/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:683125/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:2199656/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:5242445/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:246730/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:1100305/93‑1 (MQ=255)

                                                                       gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:4554543/93‑1 (MQ=255)

                                                                         tgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTtata                 <  2:5132650/93‑1 (MQ=255)

                                                                           aGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACt               <  2:3872462/93‑1 (MQ=255)

                                                                             tttGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCa             <  1:1338590/93‑1 (MQ=255)

                                                                              ttGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCAt            <  2:4037390/93‑1 (MQ=255)

                                                                               tGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATg           >  2:2981540/1‑93 (MQ=255)

                                                                               tGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATg           >  2:5733154/1‑93 (MQ=255)

                                                                                gCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa          <  2:5940863/93‑1 (MQ=255)

                                                                                gCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa          <  1:2121896/93‑1 (MQ=255)

                                                                                gCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa          >  2:6993215/1‑93 (MQ=255)

                                                                                 cTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGAc         <  2:420186/93‑1 (MQ=255)

                                                                                     cGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAACCAATCTGCGGAATATCTTTTATACTCATGACCGcc     <  2:4521724/93‑1 (MQ=255)

                                                                                     cGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGcc     <  1:4162377/93‑1 (MQ=255)

                                                                                       ccGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcct   <  2:6306751/93‑1 (MQ=255)

                                                                                        cGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcctc  <  1:2779674/93‑1 (MQ=255)

ATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTC  >  NC_000913/1870650‑1870830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`