BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,871,827	C→T	A77V (GCC→GTC)	yeaK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,871,827	0	C	T	81.6%	566.3 / 93.6	239	A77V (GCC→GTC)	yeaK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (20/24); new base T (108/87); total (128/111)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-02

Only 100 of 239 total aligned reads displayed.

TAGGCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTGTGTCTT  >  NC_000913/1871737‑1871917

tAGGCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCg                                                                                          >  2:1666523/1‑93 (MQ=255)

 aGGCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGa                                                                                         <  2:2106734/93‑1 (MQ=255)

 aGGCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGa                                                                                         <  2:3586955/93‑1 (MQ=255)

  ggCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGAt                                                                                        >  2:33031/1‑93 (MQ=255)

   gCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATc                                                                                       <  1:5350884/93‑1 (MQ=255)

    ccAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCt                                                                                      >  1:2396937/1‑93 (MQ=255)

    ccAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCt                                                                                      <  2:7200888/93‑1 (MQ=255)

       gggTGCTAAGGCGCTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTGGCAGCCGATCAACAGGTCGATCTAAg                                                                                   >  2:6897563/1‑93 (MQ=255)

       gggTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAg                                                                                   >  1:238439/1‑93 (MQ=255)

            cTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAAc                                                                              <  2:804138/93‑1 (MQ=255)

            cTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAAc                                                                              <  1:22894/93‑1 (MQ=255)

                   aCTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCgccag                                                                       >  2:3014341/1‑93 (MQ=255)

                   aCTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCgccag                                                                       >  1:7365859/1‑93 (MQ=255)

                     tGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCCCAGCCGATCAACAGGCCGATCTGAGCCAACTCgccagcc                                                                     <  1:6216359/93‑1 (MQ=255)

                      ggTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCgccagcca                                                                    <  1:3961396/93‑1 (MQ=255)

                        tATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCata                                                                  >  1:3522260/1‑93 (MQ=255)

                        tATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCata                                                                  <  2:3456429/93‑1 (MQ=255)

                         aTGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGTGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCatat                                                                 >  2:1850810/1‑93 (MQ=255)

                         aTGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCatat                                                                 >  1:4882256/1‑93 (MQ=255)

                          tGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCAtata                                                                >  2:483602/1‑93 (MQ=255)

                          tGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCAtata                                                                <  1:6596910/93‑1 (MQ=255)

                          tGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCAtata                                                                >  1:6056860/1‑93 (MQ=255)

                            tAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAgg                                                              <  1:5915455/93‑1 (MQ=255)

                             aaaGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGc                                                             <  2:4661702/93‑1 (MQ=255)

                              aaGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCg                                                            <  1:2865725/93‑1 (MQ=255)

                              aaGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCg                                                            <  2:2043405/93‑1 (MQ=255)

                                gTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGa                                                          >  1:1467236/1‑93 (MQ=255)

                                 tCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGAt                                                         <  1:3777826/93‑1 (MQ=255)

                                 tCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGAt                                                         >  1:2444052/1‑93 (MQ=255)

                                 tCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGAt                                                         <  2:4644813/93‑1 (MQ=255)

                                   aaaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTa                                                       <  2:2753078/93‑1 (MQ=255)

                                   aaaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTa                                                       <  1:4413435/93‑1 (MQ=255)

                                   aaaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTa                                                       <  2:6464601/93‑1 (MQ=255)

                                   aaaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTa                                                       <  2:2970229/93‑1 (MQ=255)

                                   aaaGGCAATGGCATAAATCAACATGATCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATAGAGTCGGagtg                                                       >  1:3205434/1‑90 (MQ=255)

                                    aaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAc                                                      <  2:1131586/93‑1 (MQ=255)

                                    aaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAc                                                      >  2:6383462/1‑93 (MQ=255)

                                    aaGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAc                                                      >  1:4174538/1‑93 (MQ=255)

                                      ggCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAcgc                                                    >  1:1881962/1‑93 (MQ=255)

                                      ggCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAcgc                                                    >  2:2498491/1‑93 (MQ=255)

                                       gCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAcgcg                                                   >  1:6318316/1‑93 (MQ=255)

                                       gCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTAcgcg                                                   >  2:1627794/1‑93 (MQ=255)

                                         aaTGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCt                                                 <  1:5518748/93‑1 (MQ=255)

                                         aaTGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCt                                                 <  2:6288082/93‑1 (MQ=255)

                                          aTGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCtt                                                >  1:5249065/1‑93 (MQ=255)

                                            ggCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCg                                              <  2:4469101/93‑1 (MQ=255)

                                             gCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGt                                             >  1:6929501/1‑93 (MQ=255)

                                              cGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGtt                                            <  2:5969674/93‑1 (MQ=255)

                                               gTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTg                                           >  2:4530402/1‑93 (MQ=255)

                                               gTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTg                                           <  2:730890/93‑1 (MQ=255)

                                               gTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTg                                           >  1:5957100/1‑93 (MQ=255)

                                                tAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTgg                                          >  1:771242/1‑93 (MQ=255)

                                                tAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTgg                                          >  1:6033922/1‑93 (MQ=255)

                                                   aTCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCa                                       >  1:3710118/1‑93 (MQ=255)

                                                    tCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAg                                      >  2:2009100/1‑93 (MQ=255)

                                                      aaCATGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGcc                                    <  2:1275943/93‑1 (MQ=255)

                                                      aaCATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGcc                                    <  1:1629226/93‑1 (MQ=255)

                                                      aaCATGTTCTGGCGACCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGcc                                    <  1:1383931/93‑1 (MQ=255)

                                                          tGTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCcggc                                >  1:809336/1‑93 (MQ=255)

                                                          tGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCcggc                                <  2:2879022/93‑1 (MQ=255)

                                                           gTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCcggcg                               <  1:6476084/93‑1 (MQ=255)

                                                           gTTCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCcggcg                               >  1:7140062/1‑93 (MQ=255)

                                                            ttCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCcggcgg                              >  1:6124267/1‑93 (MQ=255)

                                                             tCTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGa                             >  2:1713718/1‑93 (MQ=255)

                                                              cTGGCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGaa                            <  1:4677748/93‑1 (MQ=255)

                                                                ggCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGt                          <  2:7614/93‑1 (MQ=255)

                                                                ggCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGt                          >  2:549335/1‑93 (MQ=255)

                                                                ggCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGt                          >  2:6759475/1‑93 (MQ=255)

                                                                 gCGATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTc                         <  1:4599124/93‑1 (MQ=255)

                                                                   gATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGa                       <  2:149889/93‑1 (MQ=255)

                                                                   gATCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGa                       <  1:6907645/93‑1 (MQ=255)

                                                                    aTCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGAt                      >  1:1957409/1‑93 (MQ=255)

                                                                     tCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATg                     >  2:3583444/1‑93 (MQ=255)

                                                                     tCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATg                     >  2:4352735/1‑93 (MQ=255)

                                                                     tCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATg                     <  2:5786619/93‑1 (MQ=255)

                                                                     tCCTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATACAGGCGGATTACGCGCGTCGTTGGCCAGCCCGGCGGAAGACGATg                     >  2:7030670/1‑93 (MQ=255)

                                                                       cTCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGaa                   <  2:1203201/93‑1 (MQ=255)

                                                                        tCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAAc                  <  1:2953765/93‑1 (MQ=255)

                                                                        tCGCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAAc                  >  1:6842170/1‑93 (MQ=255)

                                                                          gCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTc                >  2:5052880/1‑93 (MQ=255)

                                                                          gCAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTc                <  2:6664211/93‑1 (MQ=255)

                                                                          gCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTc                <  1:4545355/93‑1 (MQ=255)

                                                                           cAGCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTca               <  2:5868186/93‑1 (MQ=255)

                                                                             gCCGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTcaca             >  1:4058373/1‑93 (MQ=255)

                                                                              ccGATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAg            >  2:5360883/1‑93 (MQ=255)

                                                                                gATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGc          <  1:2263947/93‑1 (MQ=255)

                                                                                gATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGc          >  2:7169896/1‑93 (MQ=255)

                                                                                gATCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGc          <  2:193831/93‑1 (MQ=255)

                                                                                gATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGc          <  2:1363016/93‑1 (MQ=255)

                                                                                 aTCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCt         <  1:2013956/93‑1 (MQ=255)

                                                                                 aTCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCt         >  2:1004692/1‑93 (MQ=255)

                                                                                 aTCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCt         >  1:5149376/1‑93 (MQ=255)

                                                                                  tCAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCtg        >  2:1403512/1‑93 (MQ=255)

                                                                                  tCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCtg        >  2:515451/1‑93 (MQ=255)

                                                                                  tCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCtg        >  2:2685490/1‑93 (MQ=255)

                                                                                   cAACAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCtgt       >  2:3909605/1‑93 (MQ=255)

                                                                                     aCAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTgtgt     >  1:3837364/1‑93 (MQ=255)

                                                                                      cAGGTCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTGTGTc    <  2:2296601/93‑1 (MQ=255)

                                                                                        ggCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTGTGTCtt  >  2:3095327/1‑93 (MQ=255)

                                                                                        ggCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTGTGTCtt  <  2:1484429/93‑1 (MQ=255)

TAGGCCAGGGTGCTAAGGCACTGGTATGTAAAGTCAAAGGCAATGGCGTAAATCAACATGTTCTGGCGATCCTCGCAGCCGATCAACAGGCCGATCTGAGCCAACTCGCCAGCCATATAGGCGGATTACGCGCTTCGTTGGCCAGCCCGGCGGAAGTCGATGAACTCACAGGCTGTGTCTT  >  NC_000913/1871737‑1871917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`