BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,884,922	G→A	A365V (GCA→GTA)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,884,922	0	G	A	83.2%	545.0 / 80.8	226	A365V (GCA→GTA)	rnd	RNase D
Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (99/89); total (117/109)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-02

Only 100 of 227 total aligned reads displayed.

TGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAGTTGGT  >  NC_000913/1884830‑1885012

tGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATg                                                                                             <  1:6706506/93‑1 (MQ=255)

  tAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACt                                                                                           >  1:2908899/1‑93 (MQ=255)

    gTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTc                                                                                         <  1:1915626/93‑1 (MQ=255)

    gTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTc                                                                                         <  2:6614809/93‑1 (MQ=255)

    gTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTc                                                                                         <  1:6325797/93‑1 (MQ=255)

     tttGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTcc                                                                                        >  2:941656/1‑93 (MQ=255)

     tttGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTcc                                                                                        <  1:2856988/93‑1 (MQ=255)

        gCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGcc                                                                                     >  1:5789290/1‑93 (MQ=255)

           gAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATc                                                                                  <  2:2386091/93‑1 (MQ=255)

           gAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATc                                                                                  <  1:4127220/93‑1 (MQ=255)

           gAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATc                                                                                  <  1:6042216/93‑1 (MQ=255)

            aaGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCa                                                                                 >  2:1523309/1‑93 (MQ=255)

            aaGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCa                                                                                 <  1:6064063/93‑1 (MQ=255)

             aGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAg                                                                                >  1:7342646/1‑93 (MQ=255)

              gCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGc                                                                               <  2:5060366/93‑1 (MQ=255)

              gCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGc                                                                               >  2:6985259/1‑93 (MQ=255)

               ccGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCt                                                                              >  1:985311/1‑93 (MQ=255)

                cGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTc                                                                             <  1:1473821/93‑1 (MQ=255)

                cGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTc                                                                             >  1:5632133/1‑93 (MQ=255)

                  gATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcac                                                                           <  1:436015/93‑1 (MQ=255)

                   aTGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacc                                                                          <  2:7267454/93‑1 (MQ=255)

                   aTGTGGCGCTGAGCGCGCCCAGTCCGGCTCCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacc                                                                          >  2:764273/1‑93 (MQ=255)

                    tgtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCTTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacca                                                                         <  2:7385227/93‑1 (MQ=255)

                    tgtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcacca                                                                         <  2:195182/93‑1 (MQ=255)

                    tgtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacca                                                                         <  1:6981068/93‑1 (MQ=255)

                    tgtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacca                                                                         <  1:1298916/93‑1 (MQ=255)

                    tgtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcacca                                                                         <  2:5680383/93‑1 (MQ=255)

                     gtgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTcaccac                                                                        <  1:4878419/93‑1 (MQ=255)

                      tgGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACg                                                                       >  2:3214092/1‑93 (MQ=255)

                       ggCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGc                                                                      >  1:3001289/1‑93 (MQ=255)

                         cgcTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCa                                                                    <  2:6723856/93‑1 (MQ=255)

                            tGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGATCACCACGCCAGcc                                                                 <  2:6438885/93‑1 (MQ=255)

                                 gcgcCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGaaa                                                            >  2:4977426/1‑93 (MQ=255)

                                 gcgcCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGaaa                                                            <  2:1819653/93‑1 (MQ=255)

                                  cgcCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAAt                                                           <  2:6089282/93‑1 (MQ=255)

                                      ccgTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGc                                                       <  1:5042419/91‑1 (MQ=255)

                                        gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTc                                                     <  2:1186012/93‑1 (MQ=255)

                                        gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTc                                                     >  1:6049581/1‑93 (MQ=255)

                                        gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTc                                                     >  2:34519/1‑93 (MQ=255)

                                        gTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTc                                                     >  1:7173410/1‑93 (MQ=255)

                                          ccGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCg                                                   >  1:5506345/1‑93 (MQ=255)

                                           cGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCgg                                                  <  1:5214367/93‑1 (MQ=255)

                                             gCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCa                                                <  1:1784228/93‑1 (MQ=255)

                                              cTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaa                                               >  2:2342024/1‑93 (MQ=255)

                                              cTTCAGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaa                                               >  2:4548824/1‑93 (MQ=255)

                                               ttCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAACTCAGCTCCGGCaaa                                              <  2:5185671/93‑1 (MQ=255)

                                               ttCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaaa                                              >  2:2058409/1‑93 (MQ=255)

                                               ttCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaaa                                              >  1:3223957/1‑93 (MQ=255)

                                                tCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAAt                                             >  1:1771749/1‑93 (MQ=255)

                                                   gAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGt                                          <  1:2619574/93‑1 (MQ=255)

                                                    aaGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGtt                                         >  2:845103/1‑93 (MQ=255)

                                                     aGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTc                                        >  1:6772341/1‑93 (MQ=255)

                                                     aGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTc                                        >  1:61190/1‑93 (MQ=255)

                                                     aGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTc                                        <  1:2494155/93‑1 (MQ=255)

                                                     aGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTc                                        <  2:3810395/93‑1 (MQ=255)

                                                     aGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTc                                        <  2:4027469/93‑1 (MQ=255)

                                                      gATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCt                                       >  2:4794674/1‑93 (MQ=255)

                                                      gATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCt                                       <  1:6688469/93‑1 (MQ=255)

                                                       aTTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTg                                      >  2:41907/1‑93 (MQ=255)

                                                       aTTTTACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTg                                      >  1:1445804/1‑93 (MQ=255)

                                                        ttttACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGc                                     <  2:5484283/93‑1 (MQ=255)

                                                        ttttACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGc                                     <  1:518195/93‑1 (MQ=255)

                                                        ttttACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGc                                     >  1:5136464/1‑93 (MQ=255)

                                                        ttttACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGc                                     <  2:3494792/93‑1 (MQ=255)

                                                         tttACTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCg                                    >  1:1983817/1‑93 (MQ=255)

                                                            aCTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGtt                                 >  1:7141109/1‑93 (MQ=255)

                                                            aCTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGtt                                 <  2:4422732/93‑1 (MQ=255)

                                                             cTGCGGATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGttt                                <  1:4243917/93‑1 (MQ=255)

                                                                 ggATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGt                            >  1:2193695/1‑93 (MQ=255)

                                                                 ggATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGt                            >  1:4427112/1‑93 (MQ=255)

                                                                  gATATTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGtt                           <  2:1625524/93‑1 (MQ=255)

                                                                  gATATTCCTGCAAT‑AATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGttt                          <  2:2886977/93‑1 (MQ=255)

                                                                   atatTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGttt                          >  1:3729585/1‑93 (MQ=255)

                                                                   atatTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGttt                          >  2:254811/1‑93 (MQ=255)

                                                                   atatTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGttt                          <  1:3540250/93‑1 (MQ=255)

                                                                    tatTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTc                         <  2:3984664/93‑1 (MQ=255)

                                                                     atTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTcc                        <  1:912311/93‑1 (MQ=255)

                                                                     atTCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTcc                        >  2:6060642/1‑93 (MQ=255)

                                                                      ttCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCa                       >  2:2684537/1‑93 (MQ=255)

                                                                       tCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑g                     <  2:4893610/93‑1 (MQ=255)

                                                                       tCCTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑g                     <  1:4588500/93‑1 (MQ=255)

                                                                        ccTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑Gt                    <  2:4139928/93‑1 (MQ=255)

                                                                         cTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTg                   >  1:6794747/1‑93 (MQ=255)

                                                                         cTGCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTg                   >  1:4309882/1‑93 (MQ=255)

                                                                           gCAATAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGcc                 <  1:6519492/93‑1 (MQ=255)

                                                                             aaTAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAg               >  2:5225056/1‑93 (MQ=255)

                                                                              aTAAATTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGt              >  1:1594822/1‑93 (MQ=255)

                                                                                aaaTTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTc            <  1:4684404/93‑1 (MQ=255)

                                                                                 aaTTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTca           <  1:4846511/93‑1 (MQ=255)

                                                                                  aTTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTcag          >  2:5499660/1‑93 (MQ=255)

                                                                                  aTTGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTcag          >  1:2445426/1‑93 (MQ=255)

                                                                                   ttGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTcagc         <  2:3421315/93‑1 (MQ=255)

                                                                                   ttGTGTAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTcagc         >  1:3199522/1‑93 (MQ=255)

                                                                                     gtgtAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTctgcgg       >  1:5712415/1‑91 (MQ=255)

                                                                                     gtgtAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTcagcag       >  2:57858/1‑93 (MQ=255)

                                                                                      tgtAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAgt      >  2:5869523/1‑93 (MQ=255)

                                                                                      tgtAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAgt      <  1:6262672/93‑1 (MQ=255)

                                                                                        tAATACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGGTCCAAGTTCAGCAgtt     >  1:3639993/1‑93 (MQ=255)

                                                                                         aaTACTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAgttgg   >  1:3462092/1‑93 (MQ=255)

                                                                                          aTGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAgttggt  <  2:2360339/93‑1 (MQ=255)

TGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCA‑GTGCCAGTTCAGCAGTTGGT  >  NC_000913/1884830‑1885012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`