BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,568	T→C	S287G (AGC→GGC)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,568	0	T	C	85.8%	553.3 / 81.8	232	S287G (AGC→GGC)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base T (19/14); new base C (98/101); total (117/115)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 233 total aligned reads displayed.

GTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCT  >  NC_000913/2115476‑2115659

gTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCt                                                                                             >  1:6013511/1‑93 (MQ=255)

 ttGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTg                                                                                            >  2:3238802/1‑93 (MQ=255)

   gAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTc                                                                                          <  2:1331724/93‑1 (MQ=255)

     gTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCaa                                                                                        <  1:6513612/93‑1 (MQ=255)

        catccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGc                                                                                     <  2:4719836/93‑1 (MQ=255)

        catccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGc                                                                                     <  2:4781159/93‑1 (MQ=255)

         atccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGcc                                                                                    <  1:6934744/93‑1 (MQ=255)

         atccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGcc                                                                                    <  2:1831587/93‑1 (MQ=255)

          tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGccc                                                                                   >  1:1576196/1‑93 (MQ=255)

          tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGccc                                                                                   >  2:701484/1‑93 (MQ=255)

              tcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTAc                                                                               <  1:6284795/93‑1 (MQ=255)

              tcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTAc                                                                               >  1:2564972/1‑93 (MQ=255)

                aCTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAg                                                                             <  2:2875988/93‑1 (MQ=255)

                 cTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAgg                                                                            >  2:6479873/1‑93 (MQ=255)

                 cTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAgg                                                                            <  2:2690722/93‑1 (MQ=255)

                  tACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGt                                                                           <  2:5502667/93‑1 (MQ=255)

                  tACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGt                                                                           >  2:5863027/1‑93 (MQ=255)

                  tACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGt                                                                           <  1:1532517/93‑1 (MQ=255)

                  tACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGt                                                                           >  2:741132/1‑93 (MQ=255)

                   aCGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCTGTACAGGTg                                                                          >  1:135119/1‑93 (MQ=255)

                   aCGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTg                                                                          <  2:194459/93‑1 (MQ=255)

                   aCGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTg                                                                          <  2:5601206/93‑1 (MQ=255)

                     gTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGga                                                                        <  1:1435398/93‑1 (MQ=255)

                     gTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGga                                                                        <  2:3644066/93‑1 (MQ=255)

                      tGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGgag                                                                       <  1:1010609/93‑1 (MQ=255)

                        gTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGagag                                                                     <  2:7265860/93‑1 (MQ=255)

                        gTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGagag                                                                     <  2:1218394/93‑1 (MQ=255)

                           aCGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAgcgc                                                                  <  1:4965196/93‑1 (MQ=255)

                             gggCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCaa                                                                >  1:7051698/1‑93 (MQ=255)

                             gggCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCaa                                                                >  2:3572649/1‑93 (MQ=255)

                              ggCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAAt                                                               >  1:5501915/1‑93 (MQ=255)

                               gCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATc                                                              >  2:6689777/1‑93 (MQ=255)

                               gCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATc                                                              <  1:895675/93‑1 (MQ=255)

                               gCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATc                                                              <  1:4420626/93‑1 (MQ=255)

                                  ggATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCAcc                                                           >  2:2559929/1‑93 (MQ=255)

                                  ggATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCAcc                                                           >  1:4755076/1‑93 (MQ=255)

                                  ggATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCAcc                                                           >  2:1045252/1‑93 (MQ=255)

                                   gATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCt                                                          >  1:6185114/1‑93 (MQ=255)

                                     tCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGa                                                        <  2:5301258/93‑1 (MQ=255)

                                     tCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGa                                                        >  2:1980971/1‑93 (MQ=255)

                                       gCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATa                                                      <  2:3118905/93‑1 (MQ=255)

                                       gCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATa                                                      <  1:3727359/93‑1 (MQ=255)

                                       gCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATa                                                      >  1:6881443/1‑93 (MQ=255)

                                        cTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATAc                                                     >  1:430222/1‑93 (MQ=255)

                                          gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATAccc                                                   >  2:449455/1‑93 (MQ=255)

                                          gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATAccc                                                   <  2:3466151/93‑1 (MQ=255)

                                          gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATAccc                                                   >  1:3685139/1‑93 (MQ=255)

                                          gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATAccc                                                   <  1:5945966/93‑1 (MQ=255)

                                           aTGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCt                                                  <  2:4127376/93‑1 (MQ=255)

                                           aTGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCt                                                  >  2:589569/1‑93 (MQ=255)

                                            tgtgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCtt                                                 <  2:4978500/93‑1 (MQ=255)

                                            tgtgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCtt                                                 <  1:573842/93‑1 (MQ=255)

                                              tgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCa                                               <  2:6131007/93‑1 (MQ=255)

                                              tgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCa                                               <  2:5068397/93‑1 (MQ=255)

                                                cTGGCGCAGGTTGAGCGCTACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATc                                             <  1:5328080/93‑1 (MQ=255)

                                                  ggCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCga                                           >  2:3800263/1‑93 (MQ=255)

                                                    cgcAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCgaga                                         >  1:337675/1‑93 (MQ=255)

                                                       aGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATg                                      <  1:1932707/93‑1 (MQ=255)

                                                       aGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATg                                      <  1:1729491/93‑1 (MQ=255)

                                                        ggTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGc                                     >  1:4318673/1‑93 (MQ=255)

                                                        ggTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGc                                     <  1:2264265/93‑1 (MQ=255)

                                                        ggTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGc                                     >  2:290910/1‑93 (MQ=255)

                                                         gTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCg                                    >  2:3427857/1‑93 (MQ=255)

                                                          ttGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGa                                   <  2:636647/93‑1 (MQ=255)

                                                          ttGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGa                                   <  1:3299179/93‑1 (MQ=255)

                                                           tGAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGAt                                  >  1:5759399/1‑93 (MQ=255)

                                                            gAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGAtt                                 >  1:2910647/1‑93 (MQ=255)

                                                            gAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGAtt                                 <  1:6959554/93‑1 (MQ=255)

                                                            gAGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGAtt                                 <  1:2756570/93‑1 (MQ=255)

                                                             aGCGCCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTg                                <  2:5132380/93‑1 (MQ=255)

                                                               cgcCACCATGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGac                              <  2:7319831/93‑1 (MQ=255)

                                                                 ccaccaTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGacca                            >  2:484892/1‑93 (MQ=255)

                                                                   accaTGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGaccacc                          <  1:7451261/93‑1 (MQ=255)

                                                                   accaTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGaccacc                          >  2:4291423/1‑93 (MQ=255)

                                                                    ccaTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGTTACCCTTCATCGAGAATGCGATTGACCAccc                         <  1:4490378/93‑1 (MQ=255)

                                                                     caTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCAcccc                        <  2:913903/93‑1 (MQ=255)

                                                                     caTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCAcccc                        >  2:3610746/1‑93 (MQ=255)

                                                                     caTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCAcccc                        >  1:6504938/1‑93 (MQ=255)

                                                                     caTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCAcccc                        >  1:6087972/1‑93 (MQ=255)

                                                                      aTGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCg                       <  1:4515532/93‑1 (MQ=255)

                                                                      aTGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCg                       >  2:3309815/1‑93 (MQ=255)

                                                                       tGCGGTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCgg                      >  1:1123069/1‑93 (MQ=255)

                                                                          ggTCGTCTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCaa                   >  1:5849392/1‑93 (MQ=255)

                                                                           gtcgtcTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCaaa                  <  1:1565282/93‑1 (MQ=255)

                                                                            tcgtcTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                 <  1:4084540/93‑1 (MQ=255)

                                                                              gtcTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGc               <  1:46966/93‑1 (MQ=255)

                                                                               tcTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCt              <  1:1324341/93‑1 (MQ=255)

                                                                               tcTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCt              >  1:1775044/1‑93 (MQ=255)

                                                                                cTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCtt             >  1:232198/1‑93 (MQ=255)

                                                                                cTTTGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCtt             <  1:6394842/93‑1 (MQ=255)

                                                                                 tttGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttt            <  2:6595310/93‑1 (MQ=255)

                                                                                 tttGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCTTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttt            <  1:6217636/93‑1 (MQ=255)

                                                                                 tttGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttt            >  1:3236604/1‑93 (MQ=255)

                                                                                 tttGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttt            >  2:6762926/1‑93 (MQ=255)

                                                                                 tttGTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttt            >  2:6880775/1‑93 (MQ=255)

                                                                                  ttGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCtttt           >  1:5161547/1‑93 (MQ=255)

                                                                                    gTTATAGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTc         <  1:3941237/93‑1 (MQ=255)

                                                                                      tataGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCAt       >  2:6101287/1‑93 (MQ=255)

                                                                                        taGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATAc     >  2:2260313/1‑93 (MQ=255)

                                                                                        taGCCGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATAc     >  2:3328968/1‑93 (MQ=255)

GTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCT  >  NC_000913/2115476‑2115659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`