BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,116,149	A→G	V93A (GTC→GCC)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,116,149	0	A	G	84.2%	546.3 / 96.0	246	V93A (GTC→GCC)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base A (21/18); new base G (104/103); total (125/121)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 248 total aligned reads displayed.

CTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCA  >  NC_000913/2116058‑2116239

 tGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGAcg                                                                                           >  1:3404090/1‑93 (MQ=255)

  ggATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGgcgc                                                                                          >  2:3886860/1‑93 (MQ=255)

   gATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGcgc‑g                                                                                        >  1:201092/1‑93 (MQ=255)

     tAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTg                                                                                      <  1:4685841/93‑1 (MQ=255)

     tAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTg                                                                                      >  2:6149506/1‑93 (MQ=255)

      aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTga                                                                                     >  1:7379636/1‑93 (MQ=255)

      aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTga                                                                                     >  2:4740971/1‑93 (MQ=255)

       aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgag                                                                                    <  2:9279/93‑1 (MQ=255)

       aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgag                                                                                    <  2:3458190/93‑1 (MQ=255)

       aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgag                                                                                    >  2:4812933/1‑93 (MQ=255)

        tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgaga                                                                                   <  2:6334027/93‑1 (MQ=255)

        tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgaga                                                                                   <  2:17746/93‑1 (MQ=255)

        tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTgaga                                                                                   >  2:1389624/1‑93 (MQ=255)

            gtcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCaa                                                                               >  2:235145/1‑93 (MQ=255)

             tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAAt                                                                              >  1:6529959/1‑93 (MQ=255)

             tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAAt                                                                              <  1:6784323/93‑1 (MQ=255)

              cgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATa                                                                             >  1:7161430/1‑93 (MQ=255)

               gtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATAc                                                                            <  2:3648354/93‑1 (MQ=255)

                tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATAcc                                                                           <  1:553875/93‑1 (MQ=255)

                tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATAcc                                                                           <  1:892407/93‑1 (MQ=255)

                 aaCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCt                                                                          >  2:6105584/1‑93 (MQ=255)

                  aCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTg                                                                         >  2:5495283/1‑93 (MQ=255)

                   ccTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGa                                                                        <  2:728142/93‑1 (MQ=255)

                   ccTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGa                                                                        >  2:4994844/1‑93 (MQ=255)

                    cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGAt                                                                       >  2:3237493/1‑93 (MQ=255)

                    cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGAt                                                                       <  1:2103046/93‑1 (MQ=255)

                    cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGAt                                                                       <  1:5088862/93‑1 (MQ=255)

                    cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGAt                                                                       <  1:4883612/93‑1 (MQ=255)

                    cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGAt                                                                       >  1:50597/1‑93 (MQ=255)

                     tGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATg                                                                      >  1:1290152/1‑93 (MQ=255)

                       aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCt                                                                    <  2:4766160/93‑1 (MQ=255)

                        cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTg                                                                   >  1:1165569/1‑93 (MQ=255)

                         agcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTgg                                                                  <  1:2441163/93‑1 (MQ=255)

                         agcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTgg                                                                  <  1:6854748/93‑1 (MQ=255)

                          gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGt                                                                 >  2:275240/1‑93 (MQ=255)

                          gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGt                                                                 >  2:6236281/1‑93 (MQ=255)

                          gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGt                                                                 <  1:6697166/93‑1 (MQ=255)

                           cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTa                                                                <  1:2573220/93‑1 (MQ=255)

                            agGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAg                                                               <  2:38590/93‑1 (MQ=255)

                             ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAgc                                                              <  1:4482132/93‑1 (MQ=255)

                               cgcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAgcgg                                                            >  1:3261150/1‑93 (MQ=255)

                                gcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAgcggc                                                           <  1:2204590/93‑1 (MQ=255)

                                  aCGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGa                                                         <  1:2881909/93‑1 (MQ=255)

                                   cGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGAc                                                        <  1:4441310/93‑1 (MQ=255)

                                    gAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACg                                                       >  1:4451202/1‑93 (MQ=255)

                                      aGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACgga                                                     <  2:1589317/93‑1 (MQ=255)

                                      aGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACgga                                                     <  2:1438699/93‑1 (MQ=255)

                                       gTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACggag                                                    >  2:3590112/1‑93 (MQ=255)

                                       gTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACggag                                                    <  1:5632137/93‑1 (MQ=255)

                                        tCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACggagg                                                   <  1:268120/93‑1 (MQ=255)

                                          ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAc                                                 <  1:3156962/93‑1 (MQ=255)

                                          ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAc                                                 >  2:2310149/1‑93 (MQ=255)

                                               aTCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGCGCAATACCTGATGCTGGTAGCGGCGACGGAGGACttttt                                            >  2:1998185/1‑93 (MQ=255)

                                                tCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACtttttt                                           <  2:6904795/93‑1 (MQ=255)

                                                  ccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTaa                                         <  2:845579/93‑1 (MQ=255)

                                                   cTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAc                                        >  1:3536066/1‑93 (MQ=255)

                                                     gggCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAcgc                                      >  1:863683/1‑93 (MQ=255)

                                                      ggcgatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAcgcg                                     >  2:4331011/1‑93 (MQ=255)

                                                         gatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGccc                                  >  1:1780225/1‑93 (MQ=255)

                                                            ggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                               <  1:3321192/93‑1 (MQ=255)

                                                            ggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                               >  1:6162625/1‑93 (MQ=255)

                                                             gcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACa                              <  1:541526/93‑1 (MQ=255)

                                                              cgatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACaa                             <  1:1519735/93‑1 (MQ=255)

                                                               gatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAc                            >  2:417031/1‑93 (MQ=255)

                                                               gatATTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAc                            >  1:7287521/1‑93 (MQ=255)

                                                               gatATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATGCCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAc                            <  1:5747412/93‑1 (MQ=255)

                                                               gatATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAC‑TTTTTAACGCGCCCGACAAcg                           >  2:697732/1‑93 (MQ=255)

                                                                 tatTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgc                          <  2:7173482/93‑1 (MQ=255)

                                                                  atTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcc                         >  1:1818079/1‑93 (MQ=255)

                                                                  atTGCGCAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcc                         <  1:925927/93‑1 (MQ=255)

                                                                     gcgcAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                      <  2:1963316/93‑1 (MQ=255)

                                                                     gcgcAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                      >  2:2603866/1‑93 (MQ=255)

                                                                     gcgcAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                      <  1:1454377/93‑1 (MQ=255)

                                                                       gcAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                    >  2:676231/1‑93 (MQ=255)

                                                                        cAGCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCcgccgc                   <  1:3761129/93‑1 (MQ=255)

                                                                          gCTTGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                 <  2:3387251/93‑1 (MQ=255)

                                                                            ttGCCAGTGTCGGTGGCGCGGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCt                >  1:2346787/1‑93 (MQ=255)

                                                                            ttGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtg               <  2:5806744/93‑1 (MQ=255)

                                                                             tGCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgt              <  2:4928162/93‑1 (MQ=255)

                                                                              gCCAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgtt             <  2:4471377/93‑1 (MQ=255)

                                                                               ccAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttg            <  2:6382469/93‑1 (MQ=255)

                                                                               ccAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttg            <  1:6073452/93‑1 (MQ=255)

                                                                                cAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgt           <  2:5411920/93‑1 (MQ=255)

                                                                                cAGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgt           >  2:5757327/1‑93 (MQ=255)

                                                                                 aGTGTCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg          >  2:7198238/1‑93 (MQ=255)

                                                                                 aGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg          >  1:418330/1‑93 (MQ=255)

                                                                                  gtgtCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgt         >  1:4612904/1‑93 (MQ=255)

                                                                                  gtgtCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgt         >  1:4979519/1‑93 (MQ=255)

                                                                                  gtgacggtgacgC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgt         >  1:6980101/1‑93 (MQ=255)

                                                                                   tgtCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgtt        >  2:5121630/1‑93 (MQ=255)

                                                                                   tgtCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgtt        >  2:4575493/1‑93 (MQ=255)

                                                                                   tgtCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgtt        <  1:3984301/93‑1 (MQ=255)

                                                                                     tCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTgttgtgttgt      >  2:5967480/1‑93 (MQ=255)

                                                                                     tCGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTgttgtgttgt      >  1:2843807/1‑93 (MQ=255)

                                                                                      cGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt     <  1:5519986/93‑1 (MQ=255)

                                                                                      cGGTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt     <  1:5237310/93‑1 (MQ=255)

                                                                                       ggTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGttt    >  2:29167/1‑93 (MQ=255)

                                                                                        gTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTc   <  1:3616782/93‑1 (MQ=255)

                                                                                        gTGGCGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTc   >  1:1125150/1‑93 (MQ=255)

                                                                                         tGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa  <  2:7531798/93‑1 (MQ=255)

CTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGC‑GTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCA  >  NC_000913/2116058‑2116239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`