BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,238	A→G	R479R (CGT→CGC)	b2060 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,238	0	A	G	84.8%	558.4 / 93.0	245	R479R (CGT→CGC)	b2060	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (23/14); new base G (101/106); total (124/121)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 253 total aligned reads displayed.

TGGCTTCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATT  >  NC_000913/2132147‑2132327

 ggCTTCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATcgcg                                                                                         >  2:7262782/1‑93 (MQ=255)

    ttCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCgcgcgc                                                                                      >  2:5869623/1‑93 (MQ=255)

    ttCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCgcgcgc                                                                                      <  2:2868794/93‑1 (MQ=255)

     tCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCt                                                                                     >  2:3168175/1‑93 (MQ=255)

      cAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGTGCGCtt                                                                                    <  2:6991713/93‑1 (MQ=255)

       aaTCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCttt                                                                                   <  1:946346/93‑1 (MQ=255)

        aTCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTc                                                                                  <  2:4993043/93‑1 (MQ=255)

        aTCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTc                                                                                  >  2:4576979/1‑93 (MQ=255)

        aTCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTc                                                                                  <  2:2343747/93‑1 (MQ=255)

         tCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCt                                                                                 >  1:649050/1‑93 (MQ=255)

          cGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTg                                                                                <  2:1624953/93‑1 (MQ=255)

           gCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGc                                                                               <  2:1491957/93‑1 (MQ=255)

           gCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGc                                                                               <  1:5908099/93‑1 (MQ=255)

            ccAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGcc                                                                              >  2:3301222/1‑93 (MQ=255)

             cAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCa                                                                             >  1:1268885/1‑93 (MQ=255)

             cAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCa                                                                             >  1:5607623/1‑93 (MQ=255)

              aGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCAt                                                                            >  1:891502/1‑93 (MQ=255)

              aGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCAt                                                                            >  1:3685061/1‑93 (MQ=255)

               gATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCAtt                                                                           >  1:2824224/1‑93 (MQ=255)

                aTCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTc                                                                          <  1:4233931/93‑1 (MQ=255)

                aTCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTc                                                                          >  2:1547797/1‑93 (MQ=255)

                 tCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTcc                                                                         >  2:1353209/1‑93 (MQ=255)

                   ggTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGa                                                                       >  1:5423115/1‑93 (MQ=255)

                    gTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGAc                                                                      >  1:3190125/1‑93 (MQ=255)

                    gTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGAc                                                                      <  1:6089358/93‑1 (MQ=255)

                    gTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGAc                                                                      >  2:6172727/1‑93 (MQ=255)

                    gTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGAc                                                                      >  2:7372245/1‑93 (MQ=255)

                    gTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGAc                                                                      <  1:512425/93‑1 (MQ=255)

                     ttGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACa                                                                     <  2:1168414/93‑1 (MQ=255)

                     ttGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACa                                                                     <  1:2255699/93‑1 (MQ=255)

                           tCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGa                                                               <  1:7502560/93‑1 (MQ=255)

                              ccACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGc                                                            <  1:6752706/93‑1 (MQ=255)

                               cACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCt                                                           >  1:6022521/1‑93 (MQ=255)

                               cACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCt                                                           >  2:4520185/1‑93 (MQ=255)

                                aCCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTg                                                          >  1:5017662/1‑93 (MQ=255)

                                aCCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTg                                                          <  1:1583767/93‑1 (MQ=255)

                                 ccGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTgg                                                         <  1:3119431/93‑1 (MQ=255)

                                 ccGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACACTATCCCGCGCTTTCTGCTTTTCCGACAGCGGGATGCTgg                                                         <  1:3406312/93‑1 (MQ=255)

                                     cAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCTATTCCGACAGCGGGATGCTGGCATa                                                     <  2:4896054/93‑1 (MQ=255)

                                     cAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                                                     <  1:1786721/93‑1 (MQ=255)

                                     cAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATa                                                     >  2:2400863/1‑93 (MQ=255)

                                      aGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAg                                                    >  2:4693497/1‑93 (MQ=255)

                                      aGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGATTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAg                                                    >  2:204450/1‑93 (MQ=255)

                                       gTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGa                                                   <  1:7362307/93‑1 (MQ=255)

                                        tAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGAc                                                  >  1:6395132/1‑93 (MQ=255)

                                        tAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGAc                                                  <  2:2998720/93‑1 (MQ=255)

                                        tAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGAc                                                  <  1:5325980/93‑1 (MQ=255)

                                          gCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGc                                                <  2:5646533/93‑1 (MQ=255)

                                          gCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGc                                                <  2:7490698/93‑1 (MQ=255)

                                            tGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTa                                              >  1:2724086/1‑93 (MQ=255)

                                             ggCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTaa                                             >  2:1686114/1‑93 (MQ=255)

                                              gCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAAt                                            >  2:3405606/1‑93 (MQ=255)

                                                tctGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAc                                          >  1:2064798/1‑93 (MQ=255)

                                                tctGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAc                                          >  1:4607405/1‑93 (MQ=255)

                                                tctGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAc                                          >  2:703902/1‑93 (MQ=255)

                                                 ctGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAcc                                         >  1:2541348/1‑93 (MQ=255)

                                                 ctGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATAcc                                         <  2:6090280/93‑1 (MQ=255)

                                                  tGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCg                                        >  2:6514048/1‑93 (MQ=255)

                                                   gTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgt                                       >  1:5941931/1‑93 (MQ=255)

                                                   gTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgt                                       <  1:1835843/93‑1 (MQ=255)

                                                   gTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgt                                       <  1:6320418/93‑1 (MQ=255)

                                                    tttATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgtg                                      <  1:4671465/93‑1 (MQ=255)

                                                     ttATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCgtgt                                     >  1:2585347/1‑93 (MQ=255)

                                                      tataGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGtt                                    <  2:6852615/93‑1 (MQ=255)

                                                       ataGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGttc                                   >  1:371158/1‑93 (MQ=255)

                                                       ataGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGttc                                   <  2:110337/93‑1 (MQ=255)

                                                        taGCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGttct                                  <  1:6649019/93‑1 (MQ=255)

                                                        taGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGttct                                  >  2:5531462/1‑93 (MQ=255)

                                                          gCGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGttcttc                                <  1:1221027/93‑1 (MQ=255)

                                                           cGTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTcc                               <  1:1669348/93‑1 (MQ=255)

                                                            gTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCa                              <  2:6021666/93‑1 (MQ=255)

                                                            gTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCa                              <  1:683661/93‑1 (MQ=255)

                                                            gTTTAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCa                              >  2:357781/1‑93 (MQ=255)

                                                             tttAATCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAg                             >  1:1812954/1‑93 (MQ=255)

                                                                aaTCCCTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTAc                          <  2:1458248/93‑1 (MQ=255)

                                                                aaTCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTAc                          >  1:6601016/1‑93 (MQ=255)

                                                                  tCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACtt                        >  2:6026732/1‑93 (MQ=255)

                                                                   cccTTTGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTg                       <  2:5076853/93‑1 (MQ=255)

                                                                      tttGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCgg                    <  1:5264806/93‑1 (MQ=255)

                                                                        tGATGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGc                  <  1:6311893/93‑1 (MQ=255)

                                                                           tGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCttt               <  2:4652813/93‑1 (MQ=255)

                                                                           tGGTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCttt               >  2:2241252/1‑93 (MQ=255)

                                                                            ggTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTc              >  2:3987243/1‑93 (MQ=255)

                                                                            ggTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTc              <  1:5841647/93‑1 (MQ=255)

                                                                            ggTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTc              >  1:173678/1‑93 (MQ=255)

                                                                             gTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCa             >  2:4544243/1‑93 (MQ=255)

                                                                             gTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCa             >  1:2357746/1‑93 (MQ=255)

                                                                             gTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCa             >  1:247243/1‑93 (MQ=255)

                                                                             gTTTTGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCa             >  1:2633903/1‑93 (MQ=255)

                                                                              ttttGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCaa            <  1:3534685/93‑1 (MQ=255)

                                                                              ttttGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCaa            <  2:5316835/93‑1 (MQ=255)

                                                                              ttttGACGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCaa            <  2:6282365/93‑1 (MQ=255)

                                                                              ttttGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCaa            <  1:439002/93‑1 (MQ=255)

                                                                                   aCGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCcg       >  1:2108287/1‑93 (MQ=255)

                                                                                    cGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCcgc      >  2:2545820/1‑93 (MQ=255)

                                                                                    cGCTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCcgc      >  1:3930379/1‑93 (MQ=255)

                                                                                      cTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGa    <  2:1945035/93‑1 (MQ=255)

                                                                                      cTATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGa    >  2:1910867/1‑93 (MQ=255)

                                                                                       tATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGAt   <  2:4163814/93‑1 (MQ=255)

                                                                                       tATCGCGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGAt   <  1:7130956/93‑1 (MQ=255)

TGGCTTCAATCGCCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATT  >  NC_000913/2132147‑2132327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`