BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,161,324	G→A	V143M (GTG→ATG)	baeS →	BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,161,324	0	G	A	87.2%	624.2 / 54.7	218	V143M (GTG→ATG)	baeS	BaeS
Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (71/119); total (86/132)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

Only 100 of 222 total aligned reads displayed.

AACAACAAAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCT  >  NC_000913/2161234‑2161415

aacaacAAAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATg                                                                                           >  1:2379998/1‑93 (MQ=255)

 acaacaAAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGa                                                                                          >  2:6679141/1‑93 (MQ=255)

  caacaaAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCgatgat                                                                                         <  2:2300225/93‑1 (MQ=255)

  caacaaAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCgatgat                                                                                         <  2:5156593/93‑1 (MQ=255)

   aacaaAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATc                                                                                        <  2:7168044/93‑1 (MQ=255)

   aacaaAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATc                                                                                        >  2:5315859/1‑93 (MQ=255)

      aaaGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGcc                                                                                     <  1:2855455/93‑1 (MQ=255)

       aaGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCt                                                                                    >  2:472634/1‑93 (MQ=255)

       aaGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCt                                                                                    >  2:4131348/1‑93 (MQ=255)

        aGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTc                                                                                   <  1:2222979/93‑1 (MQ=255)

         gTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTcc                                                                                  >  2:7523045/1‑93 (MQ=255)

         gTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTcc                                                                                  >  2:5614490/1‑93 (MQ=255)

         gTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTcc                                                                                  >  2:1961336/1‑93 (MQ=255)

           gCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTcccc                                                                                >  2:1085621/1‑93 (MQ=255)

           gCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTcccc                                                                                >  2:1618141/1‑93 (MQ=255)

              ggttggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGt                                                                             <  1:5603673/93‑1 (MQ=255)

              ggttggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGt                                                                             <  2:3788142/93‑1 (MQ=255)

               gttggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGtt                                                                            <  1:179076/93‑1 (MQ=255)

                ttggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTg                                                                           <  1:4563548/93‑1 (MQ=255)

                ttggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTg                                                                           <  2:2645477/93‑1 (MQ=255)

                 tggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGa                                                                          <  1:7528542/93‑1 (MQ=255)

                 tggtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGa                                                                          <  1:7355035/93‑1 (MQ=255)

                   gtCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGc                                                                        >  2:2100654/1‑93 (MQ=255)

                    tCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCg                                                                       <  1:3348271/93‑1 (MQ=255)

                      cgcgAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGt                                                                     >  2:1441360/1‑93 (MQ=255)

                         gAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTaa                                                                  <  2:4745459/93‑1 (MQ=255)

                          aGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAAc                                                                 <  2:5790885/93‑1 (MQ=255)

                          aGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAAc                                                                 >  2:6383764/1‑93 (MQ=255)

                              ccGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACgcgc                                                             >  2:7292621/1‑93 (MQ=255)

                                gATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCaa                                                           >  1:6703872/1‑93 (MQ=255)

                                gATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCaa                                                           <  2:2509673/93‑1 (MQ=255)

                                gATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCaa                                                           <  2:306255/93‑1 (MQ=255)

                                gATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCaa                                                           >  1:1399263/1‑93 (MQ=255)

                                  ttCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATa                                                         <  1:6059494/93‑1 (MQ=255)

                                  ttCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATa                                                         <  1:5018142/93‑1 (MQ=255)

                                   tCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATAc                                                        <  1:6525397/93‑1 (MQ=255)

                                    ccACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACt                                                       >  1:6850737/1‑93 (MQ=255)

                                      aCCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGa                                                     >  2:6646744/1‑93 (MQ=255)

                                      aCCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGa                                                     >  1:2561969/1‑93 (MQ=255)

                                       ccTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGat                                                    <  2:5033103/93‑1 (MQ=255)

                                       ccTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGat                                                    >  2:6118013/1‑93 (MQ=255)

                                       ccTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGat                                                    <  1:3825284/93‑1 (MQ=255)

                                       ccTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGat                                                    >  2:4970730/1‑93 (MQ=255)

                                         tGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGatat                                                  >  2:2564846/1‑93 (MQ=255)

                                            cGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCaa                                               <  2:4217750/93‑1 (MQ=255)

                                            cGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCaa                                               <  1:4945421/93‑1 (MQ=255)

                                             ggTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAAt                                              <  2:3452497/93‑1 (MQ=255)

                                               tACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAAttt                                            >  1:3529148/1‑93 (MQ=255)

                                                acacGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTc                                           <  2:5839062/93‑1 (MQ=255)

                                                    ggCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATa                                       <  2:6215461/93‑1 (MQ=255)

                                                      cGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATaaa                                     <  2:854536/93‑1 (MQ=255)

                                                        aCCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaaca                                   <  2:5722161/93‑1 (MQ=255)

                                                        aCCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaaca                                   >  2:3755561/1‑93 (MQ=255)

                                                         cccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaa                                  <  1:5994397/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 <  2:3908579/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 <  2:43309/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 >  1:117131/1‑93 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 <  2:323865/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 >  2:4827022/1‑93 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 >  2:7365950/1‑93 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 <  2:3591946/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 <  1:7050602/93‑1 (MQ=255)

                                                          ccATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAaacaac                                 >  1:1706208/1‑93 (MQ=255)

                                                           cATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAacaaca                                >  1:6963828/1‑93 (MQ=255)

                                                           cATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAacaaca                                <  1:4750879/93‑1 (MQ=255)

                                                           cATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAacaaca                                <  2:3705990/93‑1 (MQ=255)

                                                           cATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAacaaca                                <  1:7099506/93‑1 (MQ=255)

                                                           cATTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAacaaca                                <  1:1862322/93‑1 (MQ=255)

                                                            aTTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAg                               >  2:1735051/1‑93 (MQ=255)

                                                            aTTCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAg                               >  1:5344906/1‑93 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              <  1:3680001/93‑1 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              >  2:6564367/1‑93 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              >  1:2185027/1‑93 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              <  2:5529218/93‑1 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              >  2:6686651/1‑93 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              >  1:5451629/1‑93 (MQ=255)

                                                             ttCTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGc                              <  1:7234165/93‑1 (MQ=255)

                                                               cTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCgg                            <  2:2398135/93‑1 (MQ=255)

                                                               cTGGTCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCgg                            <  1:1420582/93‑1 (MQ=255)

                                                                   tCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCaaa                        <  1:3044445/93‑1 (MQ=255)

                                                                   tCAACGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCaaa                        >  2:2715710/1‑93 (MQ=255)

                                                                     aaCGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAAcc                      <  1:2018287/93‑1 (MQ=255)

                                                                      aCGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCa                     <  2:4048151/93‑1 (MQ=255)

                                                                       cGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAg                    <  2:5901874/93‑1 (MQ=255)

                                                                       cGGTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAg                    <  1:431838/93‑1 (MQ=255)

                                                                        ggTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGc                   >  2:1313844/1‑93 (MQ=255)

                                                                        ggTGCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGc                   <  2:1837414/93‑1 (MQ=255)

                                                                           gCGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTgg                <  2:2963785/93‑1 (MQ=255)

                                                                            cGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGt               <  1:1154303/93‑1 (MQ=255)

                                                                            cGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGt               <  1:4536928/93‑1 (MQ=255)

                                                                            cGGAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGt               <  2:5260254/93‑1 (MQ=255)

                                                                             ggAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGtt              <  1:6324284/93‑1 (MQ=255)

                                                                              gAAGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTg             <  1:5134308/93‑1 (MQ=255)

                                                                               aaGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGa            >  1:3405020/1‑93 (MQ=255)

                                                                               aaGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGa            >  1:638502/1‑93 (MQ=255)

                                                                               aaGATGACGCGATGATCGCCGCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGa            <  1:4780451/93‑1 (MQ=255)

                                                                                aGTTGGCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGAt           <  1:5771828/93‑1 (MQ=255)

                                                                                    ggCGCGATGATCGCCTCCCTCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTc       <  2:6834039/93‑1 (MQ=255)

                                                                                    ggCGCGATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTc       <  1:6288630/93‑1 (MQ=255)

                                                                                       gcgATGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGcc    >  1:4387852/1‑93 (MQ=255)

AACAACAAAGTGCTGGTTGGTCCGCGAGCGCCGATTCCACCTGACGGTACACGGCGACCCATTCTGGTCAACGGTGCGGAAGTTGGCGCGGTGATCGCCTCCCCCGTTGAGCGGTTAACGCGCAATACTGATATCAATTTCGATAAACAACAGCGGCAAACCAGCTGGTTGATTGTCGCCCT  >  NC_000913/2161234‑2161415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`