BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,450	G→A	R45R (CGC→CGT)	b2097 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,450	0	G	A	85.7%	734.1 / 75.3	258	R45R (CGC→CGT)	b2097	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (25/12); new base A (98/123); total (123/135)
Fisher's exact test for biased strand distribution p-value = 1.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 270 total aligned reads displayed.

CCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAA  >  NC_000913/2176358‑2176541

cccTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcggg                                                                                             >  1:6090001/1‑93 (MQ=255)

  cTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCGAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcggacg                                                                                           >  1:6220228/1‑93 (MQ=255)

  cTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcggacg                                                                                           >  2:3581993/1‑93 (MQ=255)

  cTGGTCAACCGGCAGAATAGAAAGATATCCTGTACCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTggcggacg                                                                                           >  1:2063227/1‑93 (MQ=255)

   tGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGa                                                                                          >  1:280798/1‑93 (MQ=255)

    ggTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGat                                                                                         <  2:5817333/93‑1 (MQ=255)

    ggTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGat                                                                                         <  1:3222675/93‑1 (MQ=255)

     gTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGatt                                                                                        >  1:3361409/1‑93 (MQ=255)

      tCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGatta                                                                                       <  1:1813594/93‑1 (MQ=255)

        aaCCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGattatt                                                                                     <  1:1871921/93‑1 (MQ=255)

        aaCCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGattatt                                                                                     <  1:3203133/93‑1 (MQ=255)

         aCCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTg                                                                                    <  1:3655338/93‑1 (MQ=255)

          ccGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGt                                                                                   <  1:3506168/93‑1 (MQ=255)

           cGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTc                                                                                  <  1:5524725/93‑1 (MQ=255)

            ggCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCa                                                                                 >  2:3934542/1‑93 (MQ=255)

             gCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCaa                                                                                >  2:2820934/1‑93 (MQ=255)

              cAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAAt                                                                               >  2:5534301/1‑93 (MQ=255)

              cAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAAt                                                                               <  2:6133365/93‑1 (MQ=255)

                gAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCa                                                                             >  1:7012423/1‑93 (MQ=255)

                 aaTAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCAt                                                                            >  2:6520492/1‑93 (MQ=255)

                  aTAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCAtt                                                                           >  1:5079129/1‑93 (MQ=255)

                  aTAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCAtt                                                                           >  2:940450/1‑93 (MQ=255)

                   tAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTa                                                                          <  1:4646508/93‑1 (MQ=255)

                   tAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTa                                                                          <  2:6875234/93‑1 (MQ=255)

                    aGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTAc                                                                         >  1:3739762/1‑93 (MQ=255)

                     gAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTAcg                                                                        >  1:1727301/1‑93 (MQ=255)

                      aaaGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTAcgc                                                                       <  1:1315698/93‑1 (MQ=255)

                       aaGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTAcgcg                                                                      >  1:305745/1‑93 (MQ=255)

                       aaGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTAcgcg                                                                      <  2:3868263/93‑1 (MQ=255)

                         gATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGt                                                                    >  2:3025048/1‑93 (MQ=255)

                         gATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGt                                                                    <  1:2724287/93‑1 (MQ=255)

                         gATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGt                                                                    >  1:2514648/1‑93 (MQ=255)

                          atatCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTc                                                                   <  2:1412602/93‑1 (MQ=255)

                            atCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTa                                                                 <  2:4497895/93‑1 (MQ=255)

                             tCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTAc                                                                <  1:6383875/93‑1 (MQ=255)

                              ccTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACg                                                               >  2:6800666/1‑93 (MQ=255)

                                tgtgCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTa                                                             <  1:5393114/93‑1 (MQ=255)

                                tgtgCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTa                                                             <  1:6801263/93‑1 (MQ=255)

                                  tgCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                           <  1:5990295/93‑1 (MQ=255)

                                  tgCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGt                                                           <  1:7315443/93‑1 (MQ=255)

                                    ccagccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCa                                                         <  2:2086296/93‑1 (MQ=255)

                                    ccagccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCa                                                         <  1:4499106/93‑1 (MQ=255)

                                     cagccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCAt                                                        >  1:74041/1‑93 (MQ=255)

                                     cagccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCAt                                                        <  1:978244/93‑1 (MQ=255)

                                      agccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATg                                                       >  1:4816619/1‑93 (MQ=255)

                                      agccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATg                                                       <  2:7385790/93‑1 (MQ=255)

                                       gccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGt                                                      >  2:4667763/1‑93 (MQ=255)

                                        ccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTc                                                     <  1:4659548/93‑1 (MQ=255)

                                        ccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTc                                                     <  2:4760751/93‑1 (MQ=255)

                                        ccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTc                                                     <  1:7158586/93‑1 (MQ=255)

                                        ccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTc                                                     <  2:6209872/93‑1 (MQ=255)

                                        ccagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTc                                                     <  2:1224374/93‑1 (MQ=255)

                                         cagACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTcc                                                    <  1:1067277/93‑1 (MQ=255)

                                          agACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCg                                                   >  2:107731/1‑93 (MQ=255)

                                            aCGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                 >  2:3509269/1‑93 (MQ=255)

                                            aCGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCggg                                                 >  1:8456/1‑93 (MQ=255)

                                              gCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGga                                               >  2:2336996/1‑93 (MQ=255)

                                              gCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGga                                               <  1:6439084/93‑1 (MQ=255)

                                               cccGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGgag                                              >  2:4276121/1‑93 (MQ=255)

                                                ccGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGgaga                                             >  1:2318979/1‑93 (MQ=255)

                                                  ggTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATa                                           >  1:5800524/1‑93 (MQ=255)

                                                   gtgtTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATaa                                          >  1:41509/1‑93 (MQ=255)

                                                    tgttgtACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATaaa                                         >  2:4118269/1‑93 (MQ=255)

                                                    tgttgtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATaaa                                         <  2:5174946/93‑1 (MQ=255)

                                                    tgttgtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATaaa                                         >  2:1262801/1‑93 (MQ=255)

                                                     gttgtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAg                                        >  1:3958061/1‑93 (MQ=255)

                                                      ttgtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGc                                       <  1:3478035/93‑1 (MQ=255)

                                                        gtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTg                                     <  2:2253819/93‑1 (MQ=255)

                                                        gtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTg                                     <  2:4918946/93‑1 (MQ=255)

                                                        gtACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTg                                     <  2:1292132/93‑1 (MQ=255)

                                                         tACAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTgg                                    >  1:1545706/1‑93 (MQ=255)

                                                           cAACGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTc                                  >  1:4277580/1‑93 (MQ=255)

                                                            aaCGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCa                                 >  2:3173935/1‑93 (MQ=255)

                                                            aaCGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCa                                 >  2:6168414/1‑93 (MQ=255)

                                                            aaCGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCa                                 >  2:2865088/1‑93 (MQ=255)

                                                              cGTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGa                               <  2:5865686/93‑1 (MQ=255)

                                                               gTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGTATAAGCTGGTCAGaa                              >  1:1010636/1‑93 (MQ=255)

                                                               gTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGaa                              <  1:4582798/93‑1 (MQ=255)

                                                               gTCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGaa                              >  2:3800948/1‑93 (MQ=255)

                                                                tCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAg                             >  1:3299535/1‑93 (MQ=255)

                                                                tCTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAg                             >  2:5691500/1‑93 (MQ=255)

                                                                 cTGCATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAgg                            >  1:7336219/1‑93 (MQ=255)

                                                                    cATATTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAAt                         <  1:2587214/93‑1 (MQ=255)

                                                                     atatTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAAtt                        <  2:6343446/93‑1 (MQ=255)

                                                                     atatTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAAtt                        <  2:542675/93‑1 (MQ=255)

                                                                      tatTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTg                       >  2:3279106/1‑93 (MQ=255)

                                                                      tatTACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTg                       >  1:2168249/1‑93 (MQ=255)

                                                                       atTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGt                      >  1:6597052/1‑93 (MQ=255)

                                                                        ttACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTc                     <  2:2594080/93‑1 (MQ=255)

                                                                        ttACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTc                     <  2:1963816/93‑1 (MQ=255)

                                                                         tACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCa                    >  2:862667/1‑93 (MQ=255)

                                                                         tACGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCa                    <  1:6126263/93‑1 (MQ=255)

                                                                           cGTAACACCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATa                  <  2:1809835/93‑1 (MQ=255)

                                                                               acacCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAAc              >  1:4320654/1‑93 (MQ=255)

                                                                                cacCGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACg             >  2:7243702/1‑93 (MQ=255)

                                                                                  ccGCTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGt           <  1:263842/93‑1 (MQ=255)

                                                                                     cTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCt        <  1:5467271/93‑1 (MQ=255)

                                                                                     cTGGCGGACGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCt        <  1:5189596/93‑1 (MQ=255)

                                                                                        gcggacgATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTa     >  2:6020924/1‑93 (MQ=255)

                                                                                           ggcgATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTaaaa  >  2:1467381/1‑93 (MQ=255)

CCCTGGTCAACCGGCAGAATAGAAAGATATCCTGTGCCAGCCAGACGCCCGGTGTTGTACAACGTCTGCATATTACGTAACACCGCTGGCGGGCGATTATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAA  >  NC_000913/2176358‑2176541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`