BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,184,398	A→G	A154A (GCA→GCG)	yohM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,184,398	0	A	G	86.0%	652.2 / 87.9	272	A154A (GCA→GCG)	yohM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (25/13); new base G (102/131); total (128/144)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 275 total aligned reads displayed.

GATTATGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTGGCCT  >  NC_000913/2184306‑2184487

  ttATGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGcgcg                                                                                         >  1:1222707/1‑93 (MQ=255)

   tATGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGa                                                                                        <  2:4230422/93‑1 (MQ=255)

     tGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGc                                                                                      >  2:7429622/1‑93 (MQ=255)

     tGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGc                                                                                      <  1:3966754/93‑1 (MQ=255)

     tGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGc                                                                                      >  1:4195292/1‑93 (MQ=255)

      gAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGcc                                                                                     <  1:2524784/93‑1 (MQ=255)

       aGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGccc                                                                                    >  1:4935171/1‑93 (MQ=255)

        gCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCa                                                                                   >  1:3850178/1‑93 (MQ=255)

        gCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCa                                                                                   <  2:1690377/93‑1 (MQ=255)

          atcatcatcACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATg                                                                                 <  1:7242048/93‑1 (MQ=255)

           tcatcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCGTGCGCGAGCCCATGc                                                                                >  2:7145141/1‑93 (MQ=255)

           tcatcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGc                                                                                >  2:3962130/1‑93 (MQ=255)

           tcatcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGc                                                                                >  1:4112425/1‑93 (MQ=255)

           tcatcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGc                                                                                >  1:60039/1‑93 (MQ=255)

           tcatcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGc                                                                                >  1:5960910/1‑93 (MQ=255)

            catcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGcc                                                                               <  2:2973580/93‑1 (MQ=255)

             atcatcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCa                                                                              >  2:2611819/1‑93 (MQ=255)

               catcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAAt                                                                            <  2:688772/93‑1 (MQ=255)

               catcaCGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAAt                                                                            <  2:953539/93‑1 (MQ=255)

                atcacgatcacgAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATg                                                                           >  2:1276671/1‑93 (MQ=255)

                atcacgatcacgAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATg                                                                           <  2:4820198/93‑1 (MQ=255)

                 tcacgatcacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGa                                                                          >  1:4346371/1‑93 (MQ=255)

                 tcacgatcacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGa                                                                          >  2:2769336/1‑93 (MQ=255)

                 tcacgatcacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGa                                                                          >  2:7519097/1‑93 (MQ=255)

                    cgatcacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACAt                                                                       <  2:2489403/93‑1 (MQ=255)

                       tcacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTaa                                                                    <  2:2059744/93‑1 (MQ=255)

                        cacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTaaa                                                                   <  2:4366569/93‑1 (MQ=255)

                        cacgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTaaa                                                                   <  1:3358376/93‑1 (MQ=255)

                         acgaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAac                                                                  <  1:4813334/93‑1 (MQ=255)

                           gaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAacga                                                                >  2:286141/1‑93 (MQ=255)

                           gaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAacga                                                                <  2:1721732/93‑1 (MQ=255)

                           gaACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAacga                                                                >  1:1031389/1‑93 (MQ=255)

                            aaCATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAacgac                                                               >  2:2143735/1‑93 (MQ=255)

                            aaCATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAacgac                                                               >  2:4332151/1‑93 (MQ=255)

                            aaCATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAacgac                                                               >  1:5087644/1‑93 (MQ=255)

                              cATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAacgaccc                                                             >  2:4727764/1‑91 (MQ=255)

                               aTCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCt                                                            >  1:1972445/1‑93 (MQ=255)

                               aTCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCt                                                            >  1:6194223/1‑93 (MQ=255)

                                tCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCtt                                                           <  1:4485339/93‑1 (MQ=255)

                                tCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCtt                                                           >  1:2728459/1‑93 (MQ=255)

                                  accacGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTg                                                         >  2:3559857/1‑93 (MQ=255)

                                   ccacGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGa                                                        <  2:2303335/93‑1 (MQ=255)

                                     acGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATg                                                      >  1:3775086/1‑93 (MQ=255)

                                        aCCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTa                                                   <  1:7349698/93‑1 (MQ=255)

                                        aCCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTa                                                   >  1:975604/1‑93 (MQ=255)

                                         ccATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTag                                                  <  1:4456822/93‑1 (MQ=255)

                                          cATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTaga                                                 >  2:652520/1‑93 (MQ=255)

                                          cATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTaga                                                 <  1:1755897/93‑1 (MQ=255)

                                           aTGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTagag                                                >  2:6026302/1‑93 (MQ=255)

                                            tGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTagaga                                               <  2:2415488/93‑1 (MQ=255)

                                            tGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTagaga                                               <  2:6457713/93‑1 (MQ=255)

                                            tGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTagaga                                               <  2:6314216/93‑1 (MQ=255)

                                            tGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTagaga                                               <  2:5822392/93‑1 (MQ=255)

                                              gACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAgg                                             <  2:5044551/93‑1 (MQ=255)

                                              gACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAgg                                             >  1:6712141/1‑93 (MQ=255)

                                               aCATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGt                                            >  2:1598244/1‑93 (MQ=255)

                                               aCATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGt                                            <  1:2918528/93‑1 (MQ=255)

                                                    atcaccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCa                                       <  1:576100/93‑1 (MQ=255)

                                                    atcaccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCa                                       >  1:7484759/1‑93 (MQ=255)

                                                    atcaccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCa                                       >  2:814380/1‑93 (MQ=255)

                                                       accatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACt                                    >  1:1469347/1‑93 (MQ=255)

                                                        ccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTg                                   <  2:6130279/93‑1 (MQ=255)

                                                        ccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTg                                   <  1:28446/93‑1 (MQ=255)

                                                        ccatcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTg                                   <  2:3369581/93‑1 (MQ=255)

                                                         catcacGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTgg                                  >  2:5206905/1‑93 (MQ=255)

                                                          atcacGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGc                                 >  1:1086279/1‑93 (MQ=255)

                                                           tcacGAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCa                                <  1:85868/93‑1 (MQ=255)

                                                               gAACATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAAtt                            >  2:3678649/1‑93 (MQ=255)

                                                               gAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAAtt                            >  1:6481196/1‑93 (MQ=255)

                                                                  cATGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTg                         <  1:1339469/93‑1 (MQ=255)

                                                                  cATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTg                         >  2:151238/1‑93 (MQ=255)

                                                                   aTGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGt                        >  1:3469834/1‑93 (MQ=255)

                                                                   aTGGCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGt                        <  1:514408/93‑1 (MQ=255)

                                                                    tGGCGAATATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGtt                       >  1:1715854/1‑93 (MQ=255)

                                                                      gCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAt                     <  2:5386758/93‑1 (MQ=255)

                                                                      gCGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAt                     <  2:7004899/93‑1 (MQ=255)

                                                                       cGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAtt                    <  1:2392280/93‑1 (MQ=255)

                                                                       cGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAtt                    <  2:5959907/93‑1 (MQ=255)

                                                                       cGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAtt                    <  2:7244203/93‑1 (MQ=255)

                                                                       cGAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAtt                    <  2:110588/93‑1 (MQ=255)

                                                                        gAGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTAttt                   <  1:3265673/93‑1 (MQ=255)

                                                                         aGTATCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTg                  >  2:4559303/1‑93 (MQ=255)

                                                                         aGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTg                  <  2:5875089/93‑1 (MQ=255)

                                                                             tCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCtt              <  1:3193912/93‑1 (MQ=255)

                                                                             tCAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCtt              >  2:401548/1‑93 (MQ=255)

                                                                              cAGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTa             <  2:5646521/93‑1 (MQ=255)

                                                                              cAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTa             <  2:3737550/93‑1 (MQ=255)

                                                                               aGGATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTaa            <  1:3116174/93‑1 (MQ=255)

                                                                                 gATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAAcc          <  1:7000841/93‑1 (MQ=255)

                                                                                 gATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAAcc          <  2:1063453/93‑1 (MQ=255)

                                                                                 gATGCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAAcc          >  2:465291/1‑93 (MQ=255)

                                                                                  aTGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCg         <  1:1630352/93‑1 (MQ=255)

                                                                                    gCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGt       <  1:206260/93‑1 (MQ=255)

                                                                                    gCCCATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGt       <  1:7503539/93‑1 (MQ=255)

                                                                                     cccATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTg      <  1:5351188/93‑1 (MQ=255)

                                                                                     cccATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTg      <  2:5441557/93‑1 (MQ=255)

                                                                                      ccATGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTgg     <  1:3692149/93‑1 (MQ=255)

                                                                                      ccATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTgg     >  1:5686863/1‑93 (MQ=255)

                                                                                        aTGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTGGcc   <  2:1883157/93‑1 (MQ=255)

                                                                                         tGCGCGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTGGCCt  >  2:1922391/1‑93 (MQ=255)

GATTATGAGCATCATCATCACGATCACGAACATCACCACGACCATGGACATCATCACCATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAACGACGCTTTGATGGTAGAGAGGTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTGGCCT  >  NC_000913/2184306‑2184487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`