BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,202	A→G	D5D (GAT→GAC)	yehS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,202	0	A	G	86.4%	705.6 / 96.6	287	D5D (GAT→GAC)	yehS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (18/21); new base G (123/125); total (141/146)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTTTCA  >  NC_000913/2210112‑2210294

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                            <  2:932656/93‑1 (MQ=255)

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                            >  2:1646479/1‑93 (MQ=255)

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                            <  1:3122538/93‑1 (MQ=255)

     cgGTGGCTTCGACATTGCCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTAc                                                                                       <  1:3891077/93‑1 (MQ=255)

     cgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTAc                                                                                       <  1:1148852/93‑1 (MQ=255)

     cgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTAc                                                                                       >  2:3068875/1‑93 (MQ=255)

     cgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTAc                                                                                       >  1:2762190/1‑93 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                      >  2:652572/1‑93 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                      >  1:7384333/1‑93 (MQ=255)

       gTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACtt                                                                                     >  1:6703742/1‑93 (MQ=255)

        tGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTa                                                                                    <  1:6277734/93‑1 (MQ=255)

           cTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                                 <  1:6121125/93‑1 (MQ=255)

           cTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                                 <  2:6765295/93‑1 (MQ=255)

            ttCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCAt                                                                                <  2:4574061/93‑1 (MQ=255)

            ttCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCAt                                                                                >  2:1210348/1‑93 (MQ=255)

            ttCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCAt                                                                                >  1:2865837/1‑93 (MQ=255)

            ttCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCAt                                                                                <  2:3857398/93‑1 (MQ=255)

             tCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATg                                                                               <  2:6431881/93‑1 (MQ=255)

              cGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGa                                                                              <  2:2643260/93‑1 (MQ=255)

                 cATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGc                                                                           <  1:562737/93‑1 (MQ=255)

                   ttACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCt                                                                         >  1:2238867/1‑93 (MQ=255)

                     aCCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTc                                                                       >  2:4159833/1‑93 (MQ=255)

                     aCCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTc                                                                       <  2:5264497/93‑1 (MQ=255)

                      cccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCa                                                                      <  1:1491466/93‑1 (MQ=255)

                       ccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAg                                                                     >  1:5140991/1‑93 (MQ=255)

                        cAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAgg                                                                    <  2:1982915/93‑1 (MQ=255)

                        cAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAgg                                                                    >  2:4915041/1‑93 (MQ=255)

                         aGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgt                                                                   >  2:1070306/1‑93 (MQ=255)

                             ccAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtg                                                               >  1:1245807/1‑93 (MQ=255)

                             ccAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtg                                                               >  2:4462292/1‑93 (MQ=255)

                              caaaaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtga                                                              <  1:6229604/90‑1 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                            >  1:3035259/1‑93 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                           >  2:1954792/1‑93 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                           <  2:5169847/93‑1 (MQ=255)

                                  aTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAg                                                          <  1:2661894/93‑1 (MQ=255)

                                  aTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAg                                                          <  2:6141821/93‑1 (MQ=255)

                                   tACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAgg                                                         >  1:3464611/1‑93 (MQ=255)

                                    aCGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGc                                                        <  1:7287280/93‑1 (MQ=255)

                                      gCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaa                                                      >  1:993105/1‑93 (MQ=255)

                                       cACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaa                                                     <  1:6795904/93‑1 (MQ=255)

                                        aCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaa                                                    <  1:1198952/93‑1 (MQ=255)

                                         ccAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaaa                                                   >  1:2585779/1‑93 (MQ=255)

                                          cAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAg                                                  >  1:2963789/1‑93 (MQ=255)

                                          cAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAg                                                  >  1:2454178/1‑93 (MQ=255)

                                          cAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAg                                                  >  1:272778/1‑93 (MQ=255)

                                          cAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAg                                                  >  2:6700207/1‑93 (MQ=255)

                                           aGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGc                                                 >  2:1126712/1‑93 (MQ=255)

                                            ggTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGcc                                                <  2:3251152/93‑1 (MQ=255)

                                             gTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCa                                               >  2:778211/1‑93 (MQ=255)

                                             gTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCa                                               >  2:6596507/1‑93 (MQ=255)

                                             gTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCa                                               >  2:2444706/1‑93 (MQ=255)

                                             gTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCa                                               <  1:4712713/93‑1 (MQ=255)

                                              tCATTATTATTGGCTTTCAAAATGTGGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAt                                              >  1:1440935/1‑93 (MQ=255)

                                               cATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtt                                             <  2:6987128/93‑1 (MQ=255)

                                               cATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtt                                             <  2:4732383/93‑1 (MQ=255)

                                               cATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtt                                             >  1:3981546/1‑93 (MQ=255)

                                               cATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtt                                             <  2:5864258/93‑1 (MQ=255)

                                                 ttattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtttt                                           <  2:3796230/93‑1 (MQ=255)

                                                 ttattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtttt                                           >  2:5615017/1‑93 (MQ=255)

                                                  tattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTa                                          >  2:7023234/1‑93 (MQ=255)

                                                  tattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTa                                          >  2:1375981/1‑93 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                       <  1:697195/93‑1 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                      >  2:2764891/1‑93 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                      <  1:642140/93‑1 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                      <  1:5267349/93‑1 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                     >  1:1974088/1‑93 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                     <  1:2386659/93‑1 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                    <  2:2227643/93‑1 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                    <  2:7251279/93‑1 (MQ=255)

                                                           cTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCttt                                 <  2:5979970/93‑1 (MQ=255)

                                                           cTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCttt                                 <  1:129757/93‑1 (MQ=255)

                                                            tttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtttt                                >  1:5963426/1‑93 (MQ=255)

                                                            tttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtttt                                >  1:3239030/1‑93 (MQ=255)

                                                            tttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtttt                                >  1:2838569/1‑93 (MQ=255)

                                                             ttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTa                               <  1:448280/93‑1 (MQ=255)

                                                             ttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTa                               >  2:4250520/1‑93 (MQ=255)

                                                               cAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACa                             <  2:4851340/93‑1 (MQ=255)

                                                               cAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACa                             <  1:5724758/93‑1 (MQ=255)

                                                                 aaaTGTAGCGCACGCTGCGCAGAATGTGGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAgg                           >  2:316064/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                          >  1:3677010/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                          >  1:6606238/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                          >  1:5552101/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                          <  2:466087/93‑1 (MQ=255)

                                                                     gTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCaa                       <  1:1851044/93‑1 (MQ=255)

                                                                        gcgcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCg                    >  1:3563068/1‑93 (MQ=255)

                                                                        gcgcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCg                    <  1:5337747/93‑1 (MQ=255)

                                                                          gcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGcc                  <  1:3506337/93‑1 (MQ=255)

                                                                            aCGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctc                >  1:1706976/1‑93 (MQ=255)

                                                                            aCGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctc                <  1:4188530/93‑1 (MQ=255)

                                                                             cGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctct               <  2:4624224/93‑1 (MQ=255)

                                                                             cGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctct               <  2:632636/93‑1 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt              <  1:1098882/93‑1 (MQ=255)

                                                                               cTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCttt             <  2:4962858/93‑1 (MQ=255)

                                                                               cTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCttt             >  2:3429692/1‑93 (MQ=255)

                                                                                tGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtttt            >  1:542674/1‑93 (MQ=255)

                                                                                  cgcAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTaa          >  2:3176525/1‑93 (MQ=255)

                                                                                   gcAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAg         >  2:2993532/1‑93 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt       >  1:820652/1‑93 (MQ=255)

                                                                                       aaTGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCttt     >  2:3617310/1‑93 (MQ=255)

                                                                                          atCGTTCCTTAGCATGAGCCCTTCAGTTGTGAATGGGGCAAAAAGCCATTTTGGCAGTCTTTTCCAGGCCAATCGCCTCTTTTAAGCTTTTCa  <  1:3632390/93‑1 (MQ=255)

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTTTCA  >  NC_000913/2210112‑2210294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`