BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,829	T→C	G236G (GGT→GGC)	yafD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,829	0	T	C	83.3%	628.6 / 120.4	293	G236G (GGT→GGC)	yafD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (23/26); new base C (131/113); total (154/139)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 293 total aligned reads displayed.

GCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAA  >  NC_000913/231739‑231918

  gggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGa                                                                                        <  2:7296343/93‑1 (MQ=255)

   ggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGaa                                                                                       <  1:4991863/93‑1 (MQ=255)

   ggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGaa                                                                                       >  2:4035499/1‑93 (MQ=255)

      aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGt                                                                                    <  2:2182216/93‑1 (MQ=255)

       aTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTc                                                                                   >  1:3018944/1‑93 (MQ=255)

       aTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTc                                                                                   <  2:4332287/93‑1 (MQ=255)

        tGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCa                                                                                  <  2:1478741/93‑1 (MQ=255)

         gTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAg                                                                                 <  1:3243343/93‑1 (MQ=255)

            gCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGa                                                                              <  1:5675065/93‑1 (MQ=255)

             cTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGaa                                                                             >  2:5887026/1‑93 (MQ=255)

             cTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGaa                                                                             >  2:6939156/1‑93 (MQ=255)

               gcgcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGc                                                                           >  2:7099703/1‑93 (MQ=255)

                 gcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCtt                                                                         <  1:3427108/93‑1 (MQ=255)

                 gcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCtt                                                                         <  1:1449580/93‑1 (MQ=255)

                  ccAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTc                                                                        <  2:2352495/93‑1 (MQ=255)

                  ccAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTc                                                                        <  2:1361426/93‑1 (MQ=255)

                  ccAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTc                                                                        <  1:5077274/93‑1 (MQ=255)

                   cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCt                                                                       <  1:5616835/93‑1 (MQ=255)

                     ggTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGt                                                                     >  2:2372980/1‑93 (MQ=255)

                     ggTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGt                                                                     >  1:3084262/1‑93 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                    >  2:5425724/1‑93 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                    <  2:6170476/93‑1 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                    <  1:1127466/93‑1 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                    >  1:2327738/1‑93 (MQ=255)

                      gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTa                                                                    >  1:3614437/1‑93 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACt                                                                  >  2:1052240/1‑93 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACt                                                                  <  1:2199595/93‑1 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACt                                                                  <  1:6493889/93‑1 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACt                                                                  >  1:6154008/1‑93 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACt                                                                  <  1:1642495/93‑1 (MQ=255)

                        gCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACt                                                                  >  1:5946468/1‑93 (MQ=255)

                          gTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTgg                                                                >  1:4113236/1‑93 (MQ=255)

                           ttttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGt                                                               >  1:5546661/1‑93 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                             <  2:3197777/93‑1 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                             >  2:182583/1‑93 (MQ=255)

                             ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTa                                                             >  2:4293987/1‑93 (MQ=255)

                              tACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAc                                                            >  2:1727109/1‑93 (MQ=255)

                              tACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAc                                                            <  2:1181507/93‑1 (MQ=255)

                                ccGATGATCAGCGCCGTCGGGCGGTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc                                                          >  1:3904573/1‑93 (MQ=255)

                                 cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc‑g                                                        >  1:2806384/1‑93 (MQ=255)

                                  gatgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc‑gc                                                       >  2:3797515/1‑93 (MQ=255)

                                   atgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc‑gcg                                                      >  2:2088777/1‑93 (MQ=255)

                                   atgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTAcgc‑gcg                                                      >  2:7002694/1‑93 (MQ=255)

                                     gatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCt                                                    >  2:5094062/1‑93 (MQ=255)

                                       tCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTc                                                  <  2:6689479/93‑1 (MQ=255)

                                        cAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTcc                                                 <  2:5402217/93‑1 (MQ=255)

                                          gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGa                                               <  1:4921079/93‑1 (MQ=255)

                                          gcgcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGa                                               <  1:1053524/93‑1 (MQ=255)

                                          gcgcCGTCGGGCGTGTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGa                                               >  2:6442788/1‑93 (MQ=255)

                                             ccGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATca                                            <  1:6692127/93‑1 (MQ=255)

                                             ccGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATca                                            >  1:5227709/1‑93 (MQ=255)

                                              cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATcac                                           >  1:4894928/1‑93 (MQ=255)

                                               gTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATcaca                                          >  1:4323561/1‑93 (MQ=255)

                                                tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTGCTGGTTACGC‑GCGCTTCCGATCACaa                                         <  1:6045931/93‑1 (MQ=255)

                                                tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACaa                                         <  2:2475820/93‑1 (MQ=255)

                                                tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATcacac                                         <  2:7407289/93‑2 (MQ=255)

                                                tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACaa                                         <  2:852215/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGGCGCTTCCGATCACAAt                                        >  1:2357476/1‑93 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATc                                       <  1:5472530/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATc                                       <  2:2683907/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATc                                       <  1:206151/93‑1 (MQ=255)

                                                  gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCACTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATc                                       >  1:794963/1‑93 (MQ=255)

                                                      gTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCt                                   >  1:2988275/1‑93 (MQ=255)

                                                       tttGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTa                                  <  2:2410839/93‑1 (MQ=255)

                                                       tttGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTa                                  >  1:6386207/1‑93 (MQ=255)

                                                            tCGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGt                             >  2:2576242/1‑93 (MQ=255)

                                                             cGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGtt                            <  1:4667945/93‑1 (MQ=255)

                                                             cGCCCGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGtt                            >  2:5200858/1‑93 (MQ=255)

                                                                ccGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGaa                         >  2:2608551/1‑93 (MQ=255)

                                                                 cGCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAAt                        >  2:716086/1‑93 (MQ=255)

                                                                  gCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                       <  2:2784881/93‑1 (MQ=255)

                                                                  gCTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAAtt                       >  1:7314719/1‑93 (MQ=255)

                                                                   cTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                      <  2:1674974/93‑1 (MQ=255)

                                                                   cTCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                      <  1:6811164/93‑1 (MQ=255)

                                                                    tCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCa                     >  1:4412737/1‑93 (MQ=255)

                                                                    tCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCa                     <  1:470023/93‑1 (MQ=255)

                                                                    tCGATTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCa                     >  2:6342222/1‑93 (MQ=255)

                                                                     cgtttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAAGCCGCTACTCGTTGAATTCAg                    <  1:6280785/90‑1 (MQ=255)

                                                                       aTTTTGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTc                  >  2:2985952/1‑93 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  1:4480639/93‑1 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  2:4432003/93‑1 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  2:7227623/93‑1 (MQ=255)

                                                                        ttttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTcc                 <  2:6793745/93‑1 (MQ=255)

                                                                         tttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTccc                >  2:3508840/1‑93 (MQ=255)

                                                                          ttGTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCg               >  2:5650199/1‑93 (MQ=255)

                                                                            gTTTTCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc             >  1:3477049/1‑93 (MQ=255)

                                                                                tCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         <  2:1888805/93‑1 (MQ=255)

                                                                                tCTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         <  2:274385/93‑1 (MQ=255)

                                                                                ccTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc         <  1:242886/92‑1 (MQ=255)

                                                                                 cTACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc        <  2:4859366/93‑1 (MQ=255)

                                                                                  tACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt       >  2:4022720/1‑93 (MQ=255)

                                                                                  tACCGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCt       >  1:3981058/1‑93 (MQ=255)

                                                                                    ccGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa     <  1:2820577/93‑1 (MQ=255)

                                                                                    ccGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGa     >  2:5846454/1‑93 (MQ=255)

                                                                                     cGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat    <  1:2878073/93‑1 (MQ=255)

                                                                                     cGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat    >  2:5595131/1‑93 (MQ=255)

                                                                                     cGTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat    >  2:4671866/1‑93 (MQ=255)

                                                                                      gTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata   <  1:1359120/93‑1 (MQ=255)

                                                                                      gTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata   <  2:5713914/93‑1 (MQ=255)

                                                                                      gTGGCCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata   <  2:3404295/93‑1 (MQ=255)

GCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGC‑GCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAA  >  NC_000913/231739‑231918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`