BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,917	C→T	R97H (CGC→CAC)	yeiK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,917	0	C	T	84.0%	626.5 / 82.7	245	R97H (CGC→CAC)	yeiK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (19/20); new base T (103/102); total (123/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-03

Only 100 of 251 total aligned reads displayed.

AAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGC  >  NC_000913/2252825‑2253006

aaaGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGc                                                                                           <  2:4877606/93‑1 (MQ=255)

  nncGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCtggtgg                                                                                         <  2:461736/91‑1 (MQ=255)

   gCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTggg                                                                                        <  1:4344322/93‑1 (MQ=255)

   gCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCggg                                                                                        >  1:1742225/1‑93 (MQ=255)

    cGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGt                                                                                       >  1:6076119/1‑93 (MQ=255)

    cGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGt                                                                                       <  2:6538466/93‑1 (MQ=255)

     ggACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTc                                                                                      <  2:697502/93‑1 (MQ=255)

     ggACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTc                                                                                      >  1:389228/1‑93 (MQ=255)

     ggACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATCTCACCGCATGAGTGCTTTCTGCCTGGTGGGTc                                                                                      >  2:6665885/1‑93 (MQ=255)

      gACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCa                                                                                     >  2:2172514/1‑93 (MQ=255)

      gACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCa                                                                                     >  1:2480067/1‑93 (MQ=255)

        ccAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGc                                                                                   <  1:4822011/93‑1 (MQ=255)

             cGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTc                                                                              <  1:4009699/93‑1 (MQ=255)

              ggCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCg                                                                             >  2:4159821/1‑93 (MQ=255)

                cACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGaa                                                                           >  2:2988885/1‑93 (MQ=255)

                 aCCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAAt                                                                          >  1:5786024/1‑93 (MQ=255)

                 aCCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAAt                                                                          >  1:5909193/1‑93 (MQ=255)

                  ccAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATa                                                                         >  2:1784989/1‑93 (MQ=255)

                  ccAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATa                                                                         <  2:1615795/93‑1 (MQ=255)

                  ccAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATa                                                                         >  2:6395171/1‑93 (MQ=255)

                    agagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATAcc                                                                       <  1:1494394/93‑1 (MQ=255)

                     gagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCg                                                                      <  2:3586409/93‑1 (MQ=255)

                     gagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCg                                                                      <  1:552485/93‑1 (MQ=255)

                      agTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCgg                                                                     <  2:7050978/93‑1 (MQ=255)

                      agTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCgg                                                                     >  1:4559630/1‑93 (MQ=255)

                         gATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGcc                                                                  >  2:1300914/1‑93 (MQ=255)

                         gATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGcc                                                                  >  2:3397405/1‑93 (MQ=255)

                          taatCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCa                                                                 <  1:1209904/91‑1 (MQ=255)

                           tatCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCAt                                                                >  1:616467/1‑93 (MQ=255)

                              cGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCa                                                             <  2:254988/93‑1 (MQ=255)

                              cGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCa                                                             <  2:1028512/93‑1 (MQ=255)

                              cGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCa                                                             <  2:2475680/93‑1 (MQ=255)

                                ccATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGt                                                           >  1:6488967/1‑93 (MQ=255)

                                 cATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTc                                                          <  2:442929/93‑1 (MQ=255)

                                  aTCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTcc                                                         >  1:2750344/1‑93 (MQ=255)

                                     gCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGt                                                      >  1:3260495/1‑93 (MQ=255)

                                     gCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGt                                                      <  2:5393220/93‑1 (MQ=255)

                                      cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGtt                                                     >  2:1572736/1‑93 (MQ=255)

                                      cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGtt                                                     <  2:5603869/93‑1 (MQ=255)

                                      cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGtt                                                     <  1:243775/93‑1 (MQ=255)

                                      cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCAACCAGTCCgggt                                                     >  1:3448551/1‑91 (MQ=255)

                                       ttGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGttt                                                    >  1:4627603/1‑93 (MQ=255)

                                          ccATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCAc                                                 <  2:5117294/93‑1 (MQ=255)

                                             tCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGt                                              >  2:3691592/1‑93 (MQ=255)

                                              cAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTg                                             <  1:4280764/93‑1 (MQ=255)

                                              cAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTg                                             >  2:5749660/1‑93 (MQ=255)

                                                  gTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAata                                         <  2:2403581/93‑1 (MQ=255)

                                                  gTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAata                                         <  1:3959014/93‑1 (MQ=255)

                                                  gTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAata                                         <  1:2110002/93‑1 (MQ=255)

                                                   tATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAatat                                        >  2:5955566/1‑93 (MQ=255)

                                                     tCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatta                                      >  2:3906747/1‑93 (MQ=255)

                                                      cAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtattat                                     >  2:3250047/1‑93 (MQ=255)

                                                       aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATc                                    >  1:2344280/1‑93 (MQ=255)

                                                       aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATc                                    >  1:2211916/1‑93 (MQ=255)

                                                       aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATc                                    <  1:3725673/93‑1 (MQ=255)

                                                       aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCCGTCCGGGTTTACCGTGGAtattatt                                    >  1:2541151/1‑92 (MQ=255)

                                                          gATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGc                                 >  2:3986624/1‑93 (MQ=255)

                                                          gATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGc                                 <  1:1828675/93‑1 (MQ=255)

                                                           atatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCa                                >  2:6875059/1‑93 (MQ=255)

                                                           atatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCa                                <  2:849476/93‑1 (MQ=255)

                                                            tatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                               >  2:707934/1‑93 (MQ=255)

                                                            tatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                               <  1:2994651/93‑1 (MQ=255)

                                                            tatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                               >  2:7267185/1‑93 (MQ=255)

                                                            tatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                               <  2:5482283/93‑1 (MQ=255)

                                                            tatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                               >  1:3394122/1‑93 (MQ=255)

                                                             atatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCTGCAAc                              >  1:6274203/1‑93 (MQ=255)

                                                             atatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                              >  2:3338141/1‑93 (MQ=255)

                                                             atatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                              >  2:3852408/1‑93 (MQ=255)

                                                                tttCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAt                           >  1:362284/1‑93 (MQ=255)

                                                                 ttCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAtt                          <  2:4341042/93‑1 (MQ=255)

                                                                 ttCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAtt                          <  2:2343870/93‑1 (MQ=255)

                                                                      cGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgttg                     >  2:1448940/1‑93 (MQ=255)

                                                                      cGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgttg                     <  2:7529753/93‑1 (MQ=255)

                                                                       gCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                    >  2:7182414/1‑93 (MQ=255)

                                                                       gCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                    <  2:6755793/93‑1 (MQ=255)

                                                                       gCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                    <  1:6601500/93‑1 (MQ=255)

                                                                       gCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                    >  2:5302883/1‑93 (MQ=255)

                                                                           gAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCa                <  2:3763008/93‑1 (MQ=255)

                                                                           gAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCa                <  1:7086217/93‑1 (MQ=255)

                                                                           gAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCa                >  1:1911410/1‑93 (MQ=255)

                                                                             gTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa              <  1:5314372/93‑1 (MQ=255)

                                                                             gTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa              <  1:7492666/93‑1 (MQ=255)

                                                                              tGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATaa             <  1:507592/93‑1 (MQ=255)

                                                                              tGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATaa             <  2:7379673/93‑1 (MQ=255)

                                                                              tGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATaa             >  1:7289270/1‑93 (MQ=255)

                                                                              tGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCATGAATATTATCGGCAACGATTTGTTGACGCATaa             <  1:5444124/93‑1 (MQ=255)

                                                                               gCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAAt            <  1:5111931/93‑1 (MQ=255)

                                                                                 tttCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATgg          <  1:4503445/93‑1 (MQ=255)

                                                                                  ttCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATggg         <  1:3830277/93‑1 (MQ=255)

                                                                                   tCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGc        <  1:1651485/93‑1 (MQ=255)

                                                                                   tCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGc        <  2:2923407/93‑1 (MQ=255)

                                                                                   tCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGc        <  2:259853/93‑1 (MQ=255)

                                                                                   tCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGc        <  1:2074540/93‑1 (MQ=255)

                                                                                    cTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCt       <  1:682033/93‑1 (MQ=255)

                                                                                     tGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTg      >  1:83485/1‑93 (MQ=255)

                                                                                      gCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc     >  2:5838965/1‑93 (MQ=255)

                                                                                      gCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc     <  1:2627817/93‑1 (MQ=255)

                                                                                      gCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc     >  2:1202962/1‑93 (MQ=255)

                                                                                       ccTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCg    <  1:6930964/93‑1 (MQ=255)

                                                                                         tggtggGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGc  >  1:2836687/1‑93 (MQ=255)

AAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGC  >  NC_000913/2252825‑2253006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`