BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,085	A→G	L79L (TTA→TTG)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,085	0	A	G	83.3%	573.8 / 115.2	264	L79L (TTA→TTG)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (18/26); new base G (115/105); total (133/131)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 270 total aligned reads displayed.

CGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTA  >  NC_000913/2265995‑2266177

cGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCt                                                                                             <  2:6600037/93‑1 (MQ=255)

  ccGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTtgctgc                                                                                           >  2:1531167/1‑93 (MQ=255)

  ccGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTtgctgc                                                                                           >  2:6261061/1‑93 (MQ=255)

    gATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGt                                                                                         >  1:146913/1‑93 (MQ=255)

     aTAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTc                                                                                        >  2:4660999/1‑93 (MQ=255)

     aTAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTc                                                                                        <  2:1105878/93‑1 (MQ=255)

     aTAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTc                                                                                        >  1:632431/1‑93 (MQ=255)

      tAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCa                                                                                       >  1:5446266/1‑93 (MQ=255)

      tAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCa                                                                                       <  1:2808693/93‑1 (MQ=255)

         cggcgCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAggg                                                                                    <  1:6493829/93‑1 (MQ=255)

           gcgcATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGaa                                                                                  <  2:6781265/93‑1 (MQ=255)

             gcATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGaaaa                                                                                >  2:3002519/1‑93 (MQ=255)

               aTTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAAcc                                                                              >  2:6345679/1‑93 (MQ=255)

                ttGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCa                                                                             <  2:5050699/93‑1 (MQ=255)

                  gCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGa                                                                           <  2:6967132/93‑1 (MQ=255)

                   cTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGAc                                                                          <  2:2536133/93‑1 (MQ=255)

                   cTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGAc                                                                          <  2:1459065/93‑1 (MQ=255)

                    tGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGAcc                                                                         >  1:841868/1‑93 (MQ=255)

                    tGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGAcc                                                                         >  1:7272756/1‑93 (MQ=255)

                     gAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCg                                                                        >  2:3947366/1‑93 (MQ=255)

                     gAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCg                                                                        >  2:22384/1‑93 (MQ=255)

                      aaaGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGc                                                                       <  2:5834549/93‑1 (MQ=255)

                      aaaGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGc                                                                       >  2:1261651/1‑93 (MQ=255)

                        agagATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCtt                                                                     <  2:34890/93‑1 (MQ=255)

                        agagATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCtt                                                                     <  2:91816/93‑1 (MQ=255)

                        agagATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCtt                                                                     <  2:7363186/93‑1 (MQ=255)

                        agagATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCtt                                                                     <  1:2441109/93‑1 (MQ=255)

                          agaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCttgt                                                                   <  2:1745717/93‑1 (MQ=255)

                          agaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCttgt                                                                   >  2:1419803/1‑93 (MQ=255)

                           gaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCttgtt                                                                  <  1:5966535/93‑1 (MQ=255)

                           gaTCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCttgtt                                                                  >  2:7166542/1‑93 (MQ=255)

                             tCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGa                                                                >  2:2039480/1‑93 (MQ=255)

                             tCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGa                                                                <  2:1676637/93‑1 (MQ=255)

                             tCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGa                                                                >  1:4082416/1‑93 (MQ=255)

                              ccccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGAt                                                               >  2:3808059/1‑93 (MQ=255)

                              ccccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGAt                                                               >  1:5038232/1‑93 (MQ=255)

                               cccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATa                                                              >  1:6362781/1‑93 (MQ=255)

                               cccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATa                                                              <  2:6000170/93‑1 (MQ=255)

                                ccGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATag                                                             >  2:2169969/1‑93 (MQ=255)

                                 cggcggCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATaga                                                            >  1:947500/1‑93 (MQ=255)

                                 cggcggCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATaga                                                            >  2:2839415/1‑93 (MQ=255)

                                 cggcggCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATaga                                                            <  2:3169804/93‑1 (MQ=255)

                                  ggcggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATagag                                                           <  1:7310475/93‑1 (MQ=255)

                                  ggcggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATagag                                                           <  1:1944232/93‑1 (MQ=255)

                                   gcggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGc                                                          >  1:3294711/1‑93 (MQ=255)

                                    cggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGcc                                                         <  1:7096177/93‑1 (MQ=255)

                                    cggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGcc                                                         <  2:6552093/93‑1 (MQ=255)

                                     ggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTTAGGGAAAACCAGACCGCTTGTTGATAGAGCCg                                                        <  2:4055871/93‑1 (MQ=255)

                                     ggcTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCg                                                        <  1:2863164/93‑1 (MQ=255)

                                       cTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑c                                                     <  1:4910515/93‑1 (MQ=255)

                                       cTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑c                                                     >  1:3760657/1‑93 (MQ=255)

                                        tGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑cc                                                    >  1:3672948/1‑93 (MQ=255)

                                        tGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑cc                                                    >  2:5588333/1‑93 (MQ=255)

                                        tGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑cc                                                    >  1:6616890/1‑93 (MQ=255)

                                         gCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATGCCTCGCTGGGCCAAGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCg                                                   <  1:4581808/93‑1 (MQ=255)

                                         gCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCg                                                   <  1:6113533/93‑1 (MQ=255)

                                            tgtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGc                                                >  1:774245/1‑93 (MQ=255)

                                             gtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCt                                               >  1:1188354/1‑93 (MQ=255)

                                             gtgCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCt                                               <  2:1913240/93‑1 (MQ=255)

                                              tgctgcGTTAATGGTTTTCCCAAGCAGGTAGGGTTGAATTCCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGACCCGGGCt                                               <  1:2356676/93‑1 (MQ=255)

                                              tgctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTg                                              >  2:2284542/1‑93 (MQ=255)

                                               gctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTgg                                             <  1:6240839/93‑1 (MQ=255)

                                                ctgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTggg                                            >  2:73060/1‑93 (MQ=255)

                                                 tgcGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGc                                           <  1:1794623/93‑1 (MQ=255)

                                                 tgcGGTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATACAGCCGA‑CCGGGCTGGGc                                           >  1:5678641/1‑93 (MQ=255)

                                                   cGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCa                                         <  1:3970463/93‑1 (MQ=255)

                                                    gTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCAt                                        <  1:5368785/93‑1 (MQ=255)

                                                     ttAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATc                                       >  2:5375183/1‑93 (MQ=255)

                                                       aaTGGTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCCGGGCCATCCg                                     >  1:5186428/1‑93 (MQ=255)

                                                          ggTTTACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGaaa                                  >  2:386512/1‑93 (MQ=255)

                                                            tttACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACa                                <  2:4917491/93‑1 (MQ=255)

                                                              tACCCATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGa                              <  1:1278716/93‑1 (MQ=255)

                                                                cccATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATc                            >  2:3420946/1‑93 (MQ=255)

                                                                 ccATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATcc                           <  1:1625716/93‑1 (MQ=255)

                                                                 ccATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATcc                           >  2:6504564/1‑93 (MQ=255)

                                                                  cATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCt                          >  2:6634950/1‑93 (MQ=255)

                                                                  cATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCt                          <  2:4575434/93‑1 (MQ=255)

                                                                  cATGCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCt                          >  1:4420981/1‑93 (MQ=255)

                                                                  cATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCt                          >  2:5654444/1‑93 (MQ=255)

                                                                     gCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGa                       <  1:5792034/93‑1 (MQ=255)

                                                                     gCAGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGa                       <  1:4673010/93‑1 (MQ=255)

                                                                       aGGTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATc                     >  2:2670335/1‑93 (MQ=255)

                                                                       aGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATc                     >  2:548601/1‑93 (MQ=255)

                                                                        ggTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCt                    <  2:3688186/93‑1 (MQ=255)

                                                                         gTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGTGCTGGGCCATCCGAAACAGATCCTCGATCTg                   >  2:7252730/1‑93 (MQ=255)

                                                                         gTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTg                   >  1:3349398/1‑93 (MQ=255)

                                                                         gTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTg                   >  2:5205033/1‑93 (MQ=255)

                                                                         gTAGGGTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCCAAACAGATCCTCGATCTg                   >  1:5033537/1‑93 (MQ=255)

                                                                             ggTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTaa               <  1:1909882/93‑1 (MQ=255)

                                                                             ggTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTaa               >  1:1377663/1‑93 (MQ=255)

                                                                              gTTGAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAAc              <  1:5564405/93‑1 (MQ=255)

                                                                                 gAATACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGc           >  2:7028173/1‑93 (MQ=255)

                                                                                  aaTACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCa          <  1:6806471/93‑1 (MQ=255)

                                                                                    tACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCAcc        <  1:3167116/93‑1 (MQ=255)

                                                                                    tACCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCAcc        >  2:4783521/1‑93 (MQ=255)

                                                                                     aCCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCa       <  2:3112249/93‑1 (MQ=255)

                                                                                     aCCTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCa       >  2:4330030/1‑93 (MQ=255)

                                                                                      ccTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAg      >  2:2514434/1‑93 (MQ=255)

                                                                                       cTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTTTTAACCGCACCAGt     <  2:6451733/93‑1 (MQ=255)

                                                                                       cTTGCTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGt     >  2:1352804/1‑93 (MQ=255)

CGCCGATAGCGGCGCATTGCTGAAAGAGATCCCCGGCGGCTGCATGTGCTGCGTTAATGGTTTACCCATGCAGGTAGGGTTGAATACCTTACTGCGTCAGGGAAAACCAGACCGCTTGTTGATAGAGCCGA‑CCGGGCTGGGCCATCCGAAACAGATCCTCGATCTGTTAACCGCACCAGTCTA  >  NC_000913/2265995‑2266177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`