BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,041	T→C	P309P (CCA→CCG)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,041	0	T	C	89.9%	632.2 / 55.0	237	P309P (CCA→CCG)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (12/12); new base C (107/106); total (119/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 237 total aligned reads displayed.

AAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAACTGACG  >  NC_000913/2280952‑2281130

aaaaCCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGa                                                                                        >  2:6951904/1‑93 (MQ=255)

  aaCCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATc                                                                                      <  2:7457648/93‑1 (MQ=255)

  aaCCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATc                                                                                      >  2:2893782/1‑93 (MQ=255)

   aCCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATcc                                                                                     <  2:3071423/93‑1 (MQ=255)

     cGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCa                                                                                   <  2:6882132/93‑1 (MQ=255)

     cGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCa                                                                                   <  1:3882252/93‑1 (MQ=255)

     cGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCa                                                                                   <  2:5499325/93‑1 (MQ=255)

      gATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCag                                                                                  >  1:7231198/1‑93 (MQ=255)

       aTTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCGAGGTATCGGTTGCCGGATCCCaga                                                                                 >  2:4785563/1‑93 (MQ=255)

       aTTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCaga                                                                                 >  2:3827546/1‑93 (MQ=255)

        ttAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCagaa                                                                                >  1:3677509/1‑93 (MQ=255)

          aaTTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCagaaga                                                                              <  2:2829457/93‑1 (MQ=255)

           aTTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGAt                                                                             >  2:869781/1‑93 (MQ=255)

           aTTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGAt                                                                             <  2:1612266/93‑1 (MQ=255)

            ttGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATg                                                                            >  2:5828471/1‑93 (MQ=255)

                 gccAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTc                                                                       <  1:4108123/93‑1 (MQ=255)

                 gccAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTc                                                                       <  1:4728222/93‑1 (MQ=255)

                  ccAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCa                                                                      >  1:5476413/1‑93 (MQ=255)

                    aGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCAcc                                                                    <  1:5825712/93‑1 (MQ=255)

                     gACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCt                                                                   >  1:2421887/1‑93 (MQ=255)

                       cGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTaa                                                                 <  2:4034245/93‑1 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACa                                                               >  1:3513090/1‑93 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACa                                                               <  2:4960665/93‑1 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACa                                                               >  1:1167826/1‑93 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACa                                                               >  1:6329822/1‑93 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACa                                                               <  1:2932898/93‑1 (MQ=255)

                         tCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACa                                                               >  1:2242856/1‑93 (MQ=255)

                          ccGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAg                                                              >  2:2297291/1‑93 (MQ=255)

                          ccGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAg                                                              >  2:6515275/1‑93 (MQ=255)

                            ggCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAgca                                                            >  1:7386482/1‑93 (MQ=255)

                             gcgcTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcat                                                           >  2:1050651/1‑93 (MQ=255)

                             gcgcTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGCCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcat                                                           <  1:485406/93‑1 (MQ=255)

                                cTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgc                                                        >  2:7199913/1‑93 (MQ=255)

                                cTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgc                                                        >  2:1737733/1‑93 (MQ=255)

                                cTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgc                                                        >  1:7109358/1‑93 (MQ=255)

                                 tGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca                                                       >  1:2623848/1‑93 (MQ=255)

                                 tGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca                                                       >  2:1773873/1‑93 (MQ=255)

                                 tGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca                                                       <  1:993131/93‑1 (MQ=255)

                                 tGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgca                                                       >  1:7144911/1‑93 (MQ=255)

                                  gCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgcat                                                      >  1:1064468/1‑93 (MQ=255)

                                   cAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAgcatcgcatc                                                     <  1:5331546/93‑1 (MQ=255)

                                    aaTTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCa                                                    >  1:1247999/1‑93 (MQ=255)

                                     aTTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCaa                                                   >  2:239590/1‑93 (MQ=255)

                                      ttGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCaaa                                                  <  1:876411/93‑1 (MQ=255)

                                       tGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAg                                                 <  1:335254/93‑1 (MQ=255)

                                         gTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGtt                                               >  1:2568904/1‑93 (MQ=255)

                                         gTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGtt                                               >  2:4376010/1‑93 (MQ=255)

                                         gTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGtt                                               >  1:6067153/1‑93 (MQ=255)

                                            gcgcAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAAt                                            <  1:5219560/93‑1 (MQ=255)

                                              gcAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAg                                          <  1:4227750/93‑1 (MQ=255)

                                              gcAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAg                                          <  2:752380/93‑1 (MQ=255)

                                                aaaTTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTc                                        <  1:3821204/93‑1 (MQ=255)

                                                  aTTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCaa                                      <  1:6428978/93‑1 (MQ=255)

                                                   ttCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAg                                     <  1:5883301/93‑1 (MQ=255)

                                                   ttCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAg                                     <  2:457791/93‑1 (MQ=255)

                                                    tCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGc                                    <  2:6903375/93‑1 (MQ=255)

                                                    tCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGc                                    >  1:7508392/1‑93 (MQ=255)

                                                      ggcggcGTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCa                                  <  2:207942/93‑1 (MQ=255)

                                                       gcggcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAc                                 <  2:6477398/93‑1 (MQ=255)

                                                       gcggcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAc                                 <  2:2736856/93‑1 (MQ=255)

                                                       gcggcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAc                                 <  1:7253984/93‑1 (MQ=255)

                                                          gcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAACGTTAATAGTCAAGCCAccgc                              >  2:526824/1‑93 (MQ=255)

                                                          gcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAccgc                              <  1:2137732/93‑1 (MQ=255)

                                                          gcgTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAccgc                              >  2:5979273/1‑93 (MQ=255)

                                                            gTGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCAccgccg                            <  2:1305978/93‑1 (MQ=255)

                                                             tGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACcgccgc                           <  1:4526436/93‑1 (MQ=255)

                                                             tGCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACcgccgc                           <  2:3429307/93‑1 (MQ=255)

                                                              gCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCt                          >  1:630537/1‑93 (MQ=255)

                                                              gCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGTGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCt                          <  1:3061830/93‑1 (MQ=255)

                                                              gCCTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCt                          >  1:5129136/1‑93 (MQ=255)

                                                               ccTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTa                         >  1:4630199/1‑93 (MQ=255)

                                                                cTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAc                        <  1:4741453/93‑1 (MQ=255)

                                                                cTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAc                        <  2:7148372/93‑1 (MQ=255)

                                                                cTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAc                        <  1:6452770/93‑1 (MQ=255)

                                                                cTTTAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAc                        >  2:6267681/1‑93 (MQ=255)

                                                                 tttAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAcc                       >  2:5175037/1‑93 (MQ=255)

                                                                 tttAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTAcc                       >  1:6926528/1‑93 (MQ=255)

                                                                  ttAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCg                      <  2:4917377/93‑1 (MQ=255)

                                                                  ttAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCg                      <  2:4330682/93‑1 (MQ=255)

                                                                  ttAATGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCg                      <  2:3157862/93‑1 (MQ=255)

                                                                      tGGTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCaa                  >  2:3739680/1‑93 (MQ=255)

                                                                       ggTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCaaa                 >  2:2594257/1‑93 (MQ=255)

                                                                        gTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAAc                >  1:46025/1‑93 (MQ=255)

                                                                        gTCAAGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAAc                <  1:192535/93‑1 (MQ=255)

                                                                           aaGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTc             >  2:568735/1‑93 (MQ=255)

                                                                           aaGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTc             <  2:7365183/93‑1 (MQ=255)

                                                                           aaGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTc             <  2:6665637/93‑1 (MQ=255)

                                                                            aGGTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCg            >  1:2976573/1‑93 (MQ=255)

                                                                              gTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTc          <  1:1540500/93‑1 (MQ=255)

                                                                              gTATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTc          >  1:328349/1‑93 (MQ=255)

                                                                               tATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCa         >  1:1816003/1‑93 (MQ=255)

                                                                               tATCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCa         >  2:4002463/1‑93 (MQ=255)

                                                                               tATCGGTTGCCGGAGCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCa         >  2:6441106/1‑93 (MQ=255)

                                                                                 tCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAAc       <  2:7276950/93‑1 (MQ=255)

                                                                                 tCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAAc       <  1:6655043/93‑1 (MQ=255)

                                                                                 tCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAAc       >  2:6139353/1‑93 (MQ=255)

                                                                                 tCGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAAc       >  1:5575881/1‑93 (MQ=255)

                                                                                  cGGTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAACt      <  2:1883642/93‑1 (MQ=255)

                                                                                    gTTGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAACTGa    >  2:6223949/1‑93 (MQ=255)

                                                                                      tGCCGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAACTGACg  <  2:4040154/93‑1 (MQ=255)

AAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCAGAAGATGCGTTCACCTAACAGCATCGCATCAAAGTTAATAGTCAAGCCACCGCCGCTACCGGCAAACTTCGTCAACTGACG  >  NC_000913/2280952‑2281130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`