BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,356,126	A→G	L247P (CTG→CCG)	b2245 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,356,126	0	A	G	86.7%	627.0 / 83.8	257	L247P (CTG→CCG)	b2245	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (18/16); new base G (139/83); total (158/99)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 259 total aligned reads displayed.

CAGCCGATCCCCATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCC  >  NC_000913/2356036‑2356218

  gCCGATCCCCATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGc                                                                                          <  2:2859283/93‑1 (MQ=255)

     gATCCCCATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATc                                                                                       >  2:1337214/1‑93 (MQ=255)

     gATCCCCATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATc                                                                                       >  2:3244256/1‑93 (MQ=255)

        ccccATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCt                                                                                    <  1:5701774/93‑1 (MQ=255)

         cccATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTg                                                                                   >  1:371689/1‑93 (MQ=255)

          ccATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGt                                                                                  >  2:1083062/1‑93 (MQ=255)

          ccATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGt                                                                                  >  1:3314843/1‑93 (MQ=255)

           cATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTa                                                                                 <  1:6332947/93‑1 (MQ=255)

            aTGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTag                                                                                <  1:3609429/93‑1 (MQ=255)

             tGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTaga                                                                               <  2:527410/93‑1 (MQ=255)

             tGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTaga                                                                               >  1:6672717/1‑93 (MQ=255)

                gNCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGcn                                                                            <  2:5695397/93‑2 (MQ=255)

                gCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCa                                                                            >  1:3247529/1‑93 (MQ=255)

                 ccTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCAt                                                                           <  1:2099183/93‑1 (MQ=255)

                 ccTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCAt                                                                           >  2:3997318/1‑93 (MQ=255)

                 ccTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCAt                                                                           <  2:718962/93‑1 (MQ=255)

                 ccTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCAt                                                                           >  2:1182300/1‑93 (MQ=255)

                   tttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCg                                                                         <  2:4821786/93‑1 (MQ=255)

                   tttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCg                                                                         >  1:6120083/1‑93 (MQ=255)

                    ttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCgt                                                                        <  2:4207311/93‑1 (MQ=255)

                     tGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCgtg                                                                       <  1:1216155/93‑1 (MQ=255)

                      gATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCgtgt                                                                      >  2:1626639/1‑93 (MQ=255)

                      gATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCgtgt                                                                      >  2:890857/1‑93 (MQ=255)

                        tatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCa                                                                    >  1:6706632/1‑93 (MQ=255)

                        tatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCa                                                                    >  2:5000525/1‑93 (MQ=255)

                         atCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCaa                                                                   <  1:5122205/93‑1 (MQ=255)

                          tCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCann                                                                  >  1:1518059/1‑91 (MQ=255)

                          tCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAAc                                                                  >  1:7464215/1‑93 (MQ=255)

                           cAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACg                                                                 <  2:664053/93‑1 (MQ=255)

                           cAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACg                                                                 >  2:5444861/1‑93 (MQ=255)

                           cAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAACATCGTGTCAACg                                                                 >  1:1351476/1‑93 (MQ=255)

                            aaTAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGc                                                                >  1:716354/1‑93 (MQ=255)

                              tAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCa                                                              >  2:3556792/1‑93 (MQ=255)

                              tAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCa                                                              >  1:5879902/1‑93 (MQ=255)

                              tAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCa                                                              >  2:4544068/1‑93 (MQ=255)

                               aaCTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCaa                                                             <  2:2823196/93‑1 (MQ=255)

                                aCTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAAc                                                            >  1:273638/1‑93 (MQ=255)

                                aCTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAAc                                                            <  2:4952822/93‑1 (MQ=255)

                                  tACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAg                                                          >  2:3046846/1‑93 (MQ=255)

                                  tACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAg                                                          >  2:1721846/1‑93 (MQ=255)

                                  tACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAg                                                          >  2:1029447/1‑93 (MQ=255)

                                   aCCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGc                                                         <  1:1066743/93‑1 (MQ=255)

                                    ccTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCg                                                        >  1:7382041/1‑93 (MQ=255)

                                     cTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGa                                                       <  1:6984381/93‑1 (MQ=255)

                                        ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACaa                                                    <  2:3732381/93‑1 (MQ=255)

                                         tATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCCGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACaaa                                                   >  1:49640/1‑93 (MQ=255)

                                         tATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACaaa                                                   <  2:1852053/93‑1 (MQ=255)

                                         tATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACaaa                                                   <  1:5831361/93‑1 (MQ=255)

                                          aTGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAg                                                  <  1:628238/93‑1 (MQ=255)

                                              gTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCg                                              >  1:3904190/1‑93 (MQ=255)

                                               tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGc                                             <  1:4592912/93‑1 (MQ=255)

                                               tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGc                                             >  2:7158497/1‑93 (MQ=255)

                                               tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGc                                             <  2:6459502/93‑1 (MQ=255)

                                                ggCCCATTTTTTCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCt                                            >  2:3678262/1‑93 (MQ=255)

                                                ggCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCt                                            >  2:7037590/1‑93 (MQ=255)

                                                 gCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTc                                           >  1:7033164/1‑93 (MQ=255)

                                                 gCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTc                                           <  1:2325176/93‑1 (MQ=255)

                                                  cccATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTcc                                          <  1:2257965/93‑1 (MQ=255)

                                                   ccATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTccc                                         >  1:6829244/1‑93 (MQ=255)

                                                    cATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTcccc                                        >  1:4406763/1‑93 (MQ=255)

                                                    cATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTcccc                                        >  1:4914853/1‑93 (MQ=255)

                                                    cATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTcccc                                        >  1:138955/1‑93 (MQ=255)

                                                      tttttGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTGGCTCCCCAc                                      >  2:5839714/1‑93 (MQ=255)

                                                          tGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCa                                  <  2:46144/93‑1 (MQ=255)

                                                              tGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCa                              <  2:6848005/93‑1 (MQ=255)

                                                               gATTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAt                             <  2:6528220/93‑1 (MQ=255)

                                                                aTTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAtt                            >  2:6997500/1‑93 (MQ=255)

                                                                aTTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAtt                            <  2:2149835/93‑1 (MQ=255)

                                                                aTTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAtt                            >  1:5934123/1‑93 (MQ=255)

                                                                aTTTAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAtt                            <  1:1623316/93‑1 (MQ=255)

                                                                aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCAtt                            >  2:4461787/1‑93 (MQ=255)

                                                                  ttAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATtgc                          >  2:2518387/1‑93 (MQ=255)

                                                                  ttAAACATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATtgc                          >  1:7219368/1‑93 (MQ=255)

                                                                  ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATtgc                          <  2:5251053/93‑1 (MQ=255)

                                                                     aaCATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATtgctgc                       >  2:6083422/1‑93 (MQ=255)

                                                                     aaCATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATtgctgc                       <  2:2293882/93‑1 (MQ=255)

                                                                      aCATCGCCAGTCGTTGATCCGGGGCATGGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTgcg                      >  2:5168264/1‑93 (MQ=255)

                                                                      aCATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTgcg                      >  1:5457214/1‑93 (MQ=255)

                                                                      aCATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTgcg                      >  2:1927398/1‑93 (MQ=255)

                                                                       cATCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTgcgc                     >  1:5083247/1‑93 (MQ=255)

                                                                        aTCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGcc                    >  1:2319319/1‑93 (MQ=255)

                                                                         tCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCa                   <  1:1994996/93‑1 (MQ=255)

                                                                         tCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCa                   >  2:3588607/1‑93 (MQ=255)

                                                                         tCGCCAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCa                   >  1:5668633/1‑93 (MQ=255)

                                                                            ccAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCatat                >  1:502672/1‑93 (MQ=255)

                                                                            ccAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCatat                >  1:2504594/1‑93 (MQ=255)

                                                                            ccAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCatat                >  2:2034414/1‑93 (MQ=255)

                                                                             cAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATc               >  1:3409418/1‑93 (MQ=255)

                                                                             cAGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATc               >  1:4129232/1‑93 (MQ=255)

                                                                             cAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATc               <  2:3126275/93‑1 (MQ=255)

                                                                              aGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCa              >  1:3135948/1‑93 (MQ=255)

                                                                              aGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCa              <  2:1693444/93‑1 (MQ=255)

                                                                              aGTCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCa              <  1:4025795/93‑1 (MQ=255)

                                                                                tCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAgg            <  2:6610164/93‑1 (MQ=255)

                                                                                tCGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAgg            <  1:5468142/93‑1 (MQ=255)

                                                                                 cGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGa           <  2:6167089/93‑1 (MQ=255)

                                                                                 cGTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGa           <  2:4323470/93‑1 (MQ=255)

                                                                                  gTTGATCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAg          >  2:6279868/1‑93 (MQ=255)

                                                                                       tCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCcaca     <  1:2708719/93‑1 (MQ=255)

                                                                                       tCCGGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCcaca     <  1:4576408/93‑1 (MQ=255)

CAGCCGATCCCCATGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCC  >  NC_000913/2356036‑2356218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`