BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,592	T→C	T214A (ACC→GCC)	b2248 ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,592	0	T	C	83.5%	600.1 / 108.5	274	T214A (ACC→GCC)	b2248	putative regulator
Reads supporting (aligned to +/- strand): ref base T (22/23); new base C (139/89); total (162/112)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 274 total aligned reads displayed.

CCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCG  >  NC_000913/2359502‑2359682

  cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGc                                                                                        >  1:5144777/1‑93 (MQ=255)

  cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGc                                                                                        >  2:4298428/1‑93 (MQ=255)

  cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGc                                                                                        <  1:2006191/93‑1 (MQ=255)

  cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGc                                                                                        >  2:6695479/1‑93 (MQ=255)

  cTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGc                                                                                        >  2:6230102/1‑93 (MQ=255)

   tGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCt                                                                                       >  2:1257351/1‑93 (MQ=255)

   tGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCt                                                                                       <  1:193512/93‑1 (MQ=255)

    ggCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTc                                                                                      <  1:476697/93‑1 (MQ=255)

      cgcAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGt                                                                                    <  1:4217843/93‑1 (MQ=255)

      cgcAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGt                                                                                    <  2:2464871/93‑1 (MQ=255)

      cgcAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGt                                                                                    <  1:77304/93‑1 (MQ=255)

          ggCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTAtt                                                                                <  2:2957257/93‑1 (MQ=255)

          ggCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTGAtt                                                                                >  2:4891752/1‑93 (MQ=255)

           gCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTg                                                                               >  2:7350302/1‑93 (MQ=255)

            cAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTgg                                                                              >  2:7080235/1‑93 (MQ=255)

            cAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTgg                                                                              >  1:3068820/1‑93 (MQ=255)

             aaTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGc                                                                             <  1:4333784/93‑1 (MQ=255)

             aaTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGc                                                                             >  2:6720073/1‑93 (MQ=255)

              aTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCg                                                                            >  1:7120208/1‑93 (MQ=255)

              aTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCg                                                                            >  1:6529529/1‑93 (MQ=255)

              aTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCg                                                                            >  2:3054481/1‑93 (MQ=255)

               tGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGt                                                                           <  1:3397854/93‑1 (MQ=255)

                ggCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGtt                                                                          >  1:1576366/1‑93 (MQ=255)

                 gCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTa                                                                         >  1:852365/1‑93 (MQ=255)

                 gCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTa                                                                         >  1:4890594/1‑93 (MQ=255)

                 gCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTa                                                                         >  1:1227711/1‑93 (MQ=255)

                  ccTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTaa                                                                        <  2:3230859/93‑1 (MQ=255)

                    ttGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAac                                                                      <  2:6699117/93‑1 (MQ=255)

                     ttgccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAaca                                                                     >  1:906129/4‑93 (MQ=255)

                       cccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACAcc                                                                   <  1:3477730/93‑1 (MQ=255)

                       cccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACAcc                                                                   <  2:5327346/93‑1 (MQ=255)

                        ccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCg                                                                  <  2:6857518/93‑1 (MQ=255)

                         cGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCgg                                                                 <  2:5709079/93‑1 (MQ=255)

                         cGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCgg                                                                 >  1:1053174/1‑93 (MQ=255)

                          gCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGgc                                                                >  2:1903792/1‑93 (MQ=255)

                          gCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGTCGTTAAACACCGgc                                                                >  2:4378283/1‑93 (MQ=255)

                          gCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGgc                                                                >  1:5655511/1‑93 (MQ=255)

                             aGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCa                                                             >  2:3128765/1‑93 (MQ=255)

                             aGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCa                                                             >  2:800007/1‑93 (MQ=255)

                              gATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAg                                                            <  1:3345293/93‑1 (MQ=255)

                               aTAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGc                                                           <  1:5838206/93‑1 (MQ=255)

                                   tCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGaca                                                       >  2:3882233/1‑93 (MQ=255)

                                   tCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGaca                                                       >  1:5236666/1‑93 (MQ=255)

                                      cGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACaa                                                    <  1:6148151/93‑1 (MQ=255)

                                      cGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACaa                                                    <  1:3214447/93‑1 (MQ=255)

                                      cGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACaa                                                    <  2:3629674/93‑1 (MQ=255)

                                      cGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACaa                                                    <  2:3199357/93‑1 (MQ=255)

                                       ggTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAAc                                                   >  2:416996/1‑93 (MQ=255)

                                        gTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACg                                                  >  2:2894068/1‑93 (MQ=255)

                                        gTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACg                                                  >  1:5089312/1‑93 (MQ=255)

                                        gTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACg                                                  >  1:1026087/1‑93 (MQ=255)

                                         tATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACgg                                                 >  1:142969/1‑93 (MQ=255)

                                         tATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACgg                                                 <  1:3695781/93‑1 (MQ=255)

                                         tATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGAAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACgg                                                 >  2:1292026/1‑93 (MQ=255)

                                          aTTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGa                                                >  1:2208366/1‑93 (MQ=255)

                                          aTTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGa                                                <  2:4809805/93‑1 (MQ=255)

                                          aTTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGa                                                <  1:2760890/93‑1 (MQ=255)

                                           ttcttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGAc                                               >  2:6527311/1‑93 (MQ=255)

                                           ttcttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGAc                                               >  2:3943618/1‑93 (MQ=255)

                                           ttcttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGAc                                               >  2:1660127/1‑93 (MQ=255)

                                              ttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATc                                            <  2:1353951/93‑1 (MQ=255)

                                              ttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATc                                            >  2:4991898/1‑93 (MQ=255)

                                              ttcATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATc                                            >  2:7190451/1‑93 (MQ=255)

                                                cATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCgg                                          <  1:6662706/93‑1 (MQ=255)

                                                 aTTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGc                                         >  2:513601/1‑93 (MQ=255)

                                                 aTTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGc                                         >  2:3728510/1‑93 (MQ=255)

                                                  ttGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCg                                        >  1:6753465/1‑93 (MQ=255)

                                                  ttGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCg                                        >  2:4624802/1‑93 (MQ=255)

                                                     aTTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTaa                                     >  2:1449635/1‑93 (MQ=255)

                                                     aTTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTaa                                     <  1:2502243/93‑1 (MQ=255)

                                                      tttGTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAAt                                    <  1:6099343/93‑1 (MQ=255)

                                                         gTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCtt                                 <  2:5451502/93‑1 (MQ=255)

                                                         gTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCtt                                 >  1:6192093/1‑93 (MQ=255)

                                                         gTAAGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCtt                                 <  1:3981988/93‑1 (MQ=255)

                                                          tAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTc                                <  1:7273060/93‑1 (MQ=255)

                                                           aaGCGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCg                               <  1:3000525/93‑1 (MQ=255)

                                                              cGGGTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCg                            >  1:3873313/1‑93 (MQ=255)

                                                               gggTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGcccg                           >  1:2427563/1‑91 (MQ=255)

                                                                 gTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTg                         >  2:2166353/1‑93 (MQ=255)

                                                                 gTACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTg                         <  2:6039083/93‑1 (MQ=255)

                                                                  tACCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGt                        >  2:3505146/1‑93 (MQ=255)

                                                                   aCCGACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTc                       >  1:7415405/1‑93 (MQ=255)

                                                                    ccGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCa                      <  1:7498824/93‑1 (MQ=255)

                                                                      gACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATa                    >  2:2596709/1‑93 (MQ=255)

                                                                      gACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATa                    >  1:6012193/1‑93 (MQ=255)

                                                                      gACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATa                    <  1:3137030/93‑1 (MQ=255)

                                                                      gACCACCGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATa                    >  1:1694082/1‑93 (MQ=255)

                                                                        ccaccGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGc                  >  2:2398362/1‑93 (MQ=255)

                                                                         caccGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCt                 >  1:2359465/1‑93 (MQ=255)

                                                                          accGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc                >  1:5362534/1‑93 (MQ=255)

                                                                           ccGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTcc               <  2:3309368/93‑1 (MQ=255)

                                                                           ccGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTcc               >  1:1454548/1‑93 (MQ=255)

                                                                            cGAGATCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCa              <  2:1069350/93‑1 (MQ=255)

                                                                                aTCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGccc          <  1:4534241/93‑1 (MQ=255)

                                                                                aTCGCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGccc          >  2:1698958/1‑93 (MQ=255)

                                                                                   gCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGa       <  2:6169426/93‑1 (MQ=255)

                                                                                   gCAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGa       >  2:2686499/1‑93 (MQ=255)

                                                                                    cAGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGAc      <  1:5625383/93‑1 (MQ=255)

                                                                                     aGCGGCAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACg     >  1:2242141/1‑93 (MQ=255)

                                                                                        ggTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCg  >  1:741130/1‑93 (MQ=255)

CCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCG  >  NC_000913/2359502‑2359682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`