BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,218	A→G	V132A (GTG→GCG)	yfbB ←	hyptothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,218	0	A	G	84.9%	584.6 / 88.1	253	V132A (GTG→GCG)	yfbB	hyptothetical protein
Reads supporting (aligned to +/- strand): ref base A (19/19); new base G (105/109); total (125/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 255 total aligned reads displayed.

CGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCC  >  NC_000913/2375128‑2375310

 ggCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGccc                                                                                           >  2:4917519/1‑93 (MQ=255)

 ggCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGccc                                                                                           <  1:7407183/93‑1 (MQ=255)

 ggCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTCCTGTGAGAAAACGCTGCGccc                                                                                           >  1:5465158/1‑93 (MQ=255)

  gCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCa                                                                                          <  2:1780102/93‑1 (MQ=255)

    gTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCAtt                                                                                        <  2:6469387/93‑1 (MQ=255)

    gTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCAtt                                                                                        <  1:3372467/93‑1 (MQ=255)

     ttGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTg                                                                                       >  2:2595135/1‑93 (MQ=255)

     ttGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTg                                                                                       <  2:5666812/93‑1 (MQ=255)

     ttGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTg                                                                                       >  1:7281124/1‑93 (MQ=255)

      tGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTgg                                                                                      <  1:1770388/93‑1 (MQ=255)

       gATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATTCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGc                                                                                     <  1:4600652/93‑1 (MQ=255)

       gATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGc                                                                                     <  2:5099120/93‑1 (MQ=255)

       gATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGc                                                                                     <  2:3201267/93‑1 (MQ=255)

       gATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGc                                                                                     >  1:2003633/1‑93 (MQ=255)

       gATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGc                                                                                     <  2:549600/93‑1 (MQ=255)

         tCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGa                                                                                   >  2:6198907/1‑93 (MQ=255)

          cGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGAt                                                                                  >  1:3438718/1‑93 (MQ=255)

           gTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATc                                                                                 <  1:4830551/93‑1 (MQ=255)

            tCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCg                                                                                >  2:658698/1‑93 (MQ=255)

              aTTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGa                                                                              >  1:4146254/1‑93 (MQ=255)

              aTTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGa                                                                              <  1:6490040/93‑1 (MQ=255)

              aTTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGa                                                                              >  2:4283282/1‑93 (MQ=255)

               ttGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGaa                                                                             >  2:717024/1‑93 (MQ=255)

               ttGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGaa                                                                             <  2:1254035/93‑1 (MQ=255)

                tgagtgagGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAAc                                                                            >  1:6121795/1‑93 (MQ=255)

                 gagtgagGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACg                                                                           >  1:3018421/1‑93 (MQ=255)

                   gtgagGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCt                                                                         >  2:833388/1‑93 (MQ=255)

                      agGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGAc                                                                      >  1:3136539/1‑93 (MQ=255)

                      agGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTCAc                                                                      <  2:4737337/93‑1 (MQ=255)

                          aaaaaCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTc                                                                  <  1:5595760/93‑1 (MQ=255)

                           aaaaCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTcc                                                                 <  2:4074876/93‑1 (MQ=255)

                            aaaCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCg                                                                >  2:1620114/1‑93 (MQ=255)

                              aCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCa                                                              <  1:4508340/93‑1 (MQ=255)

                              aCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCa                                                              >  1:5729836/1‑93 (MQ=255)

                              aCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCa                                                              <  2:5513254/93‑1 (MQ=255)

                              aCAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCa                                                              >  1:2876683/1‑93 (MQ=255)

                               cAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCAc                                                             <  2:7226791/93‑1 (MQ=255)

                               cAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCAc                                                             >  1:1406535/1‑93 (MQ=255)

                                aGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACg                                                            >  1:840379/1‑93 (MQ=255)

                                    tGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACgttgt                                                        <  2:4789022/93‑1 (MQ=255)

                                     gTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACgttgtt                                                       >  2:2358319/1‑93 (MQ=255)

                                     gTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACgttgtt                                                       >  1:6520167/1‑93 (MQ=255)

                                       tGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCa                                                     <  2:4421212/93‑1 (MQ=255)

                                        gATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAg                                                    <  2:1489372/93‑1 (MQ=255)

                                          tACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCa                                                  <  2:3819237/93‑1 (MQ=255)

                                          tACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCa                                                  >  2:128325/1‑93 (MQ=255)

                                          tACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCa                                                  >  1:4196124/1‑93 (MQ=255)

                                            ccAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtt                                                <  1:592895/93‑1 (MQ=255)

                                            ccAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtt                                                <  1:6769319/93‑1 (MQ=255)

                                            ccAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtt                                                <  2:5810730/93‑1 (MQ=255)

                                            ccAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtt                                                <  1:7451613/93‑1 (MQ=255)

                                             cAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAttt                                               >  1:4695734/1‑93 (MQ=255)

                                             cAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAttt                                               <  2:6141901/93‑1 (MQ=255)

                                              aGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtttt                                              <  1:7211378/93‑1 (MQ=255)

                                              aGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCAtttt                                              >  1:3757959/1‑93 (MQ=255)

                                                tCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGc                                            <  2:3454359/93‑1 (MQ=255)

                                                tCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGc                                            >  1:4273924/1‑93 (MQ=255)

                                                tCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGc                                            <  1:2699156/93‑1 (MQ=255)

                                                   gCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGc                                         >  2:2223158/1‑93 (MQ=255)

                                                    cAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGcc                                        >  2:7358503/1‑93 (MQ=255)

                                                    cAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGcc                                        <  1:6611644/93‑1 (MQ=255)

                                                     aaaTACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGccc                                       >  2:2116115/1‑93 (MQ=255)

                                                     aaaTACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGccc                                       >  2:366093/1‑93 (MQ=255)

                                                      aaTACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGcccc                                      <  2:5352703/93‑1 (MQ=255)

                                                      aaTACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGcccc                                      >  1:156642/1‑93 (MQ=255)

                                                      aaTACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTACGCACGTTGTTCAGCATTTTGCAGcacc                                      >  2:3784010/1‑93 (MQ=255)

                                                        tACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCgg                                    >  2:6836493/1‑93 (MQ=255)

                                                        tACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCgg                                    <  2:6666666/93‑1 (MQ=255)

                                                         aCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGa                                   <  1:4385/93‑1 (MQ=255)

                                                          ccgccgTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGAt                                  <  1:1133192/93‑1 (MQ=255)

                                                          ccgccgTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGAt                                  <  2:6957811/93‑1 (MQ=255)

                                                            gccgTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGc                                <  1:6052555/93‑1 (MQ=255)

                                                             ccgTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGcc                               <  2:5969993/93‑1 (MQ=255)

                                                             ccgTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGcc                               <  1:247711/93‑1 (MQ=255)

                                                             ccgTTAACGGATCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGcc                               >  2:7282850/1‑93 (MQ=255)

                                                              cgTTAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccc                              <  1:434117/93‑1 (MQ=255)

                                                                 tAACGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGcc                           <  1:2211636/93‑1 (MQ=255)

                                                                  aaCGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCt                          >  2:283972/1‑93 (MQ=255)

                                                                  aaCGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCt                          >  2:3097357/1‑93 (MQ=255)

                                                                   aCGGTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCtt                         <  2:192579/93‑1 (MQ=255)

                                                                     ggTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCg                       >  1:4986895/1‑93 (MQ=255)

                                                                     ggTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCg                       >  2:5598848/1‑93 (MQ=255)

                                                                      gTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGa                      >  1:1862636/1‑93 (MQ=255)

                                                                      gTTCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGa                      >  1:3988237/1‑93 (MQ=255)

                                                                      gTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGa                      >  1:2599714/1‑93 (MQ=255)

                                                                       ttCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGAc                     >  1:5842064/1‑93 (MQ=255)

                                                                       ttCTGTAAGAAAAAGCTACCCCCATTGGCGATCGAAACGCTGACGTTCCGCACGTTGATCAGCATTTTGCAAACCCGGATGCCCGCCTTCGAc                     >  1:3825591/1‑93 (MQ=255)

                                                                        tCTGTGAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGAGGCCCGCCTTCGACa                    >  2:4378065/1‑93 (MQ=255)

                                                                           gtgAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATa                 >  1:1108085/1‑93 (MQ=255)

                                                                           gtgAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATa                 <  2:2877151/93‑1 (MQ=255)

                                                                           gtgAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATa                 >  1:6232120/1‑93 (MQ=255)

                                                                           gtgAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATa                 <  2:1073015/93‑1 (MQ=255)

                                                                            tgAGAAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATaa                <  2:3467607/93‑1 (MQ=255)

                                                                             gagaAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAAc               >  2:1428968/1‑93 (MQ=255)

                                                                              agaAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAAcc              <  1:5074880/93‑1 (MQ=255)

                                                                               gaAAACGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAAccc             <  1:2497936/93‑1 (MQ=255)

                                                                                aaaaCGCTGCGCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAAcccc            <  1:6458582/93‑1 (MQ=255)

                                                                                aaaaCGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAAcccc            <  1:5249178/93‑1 (MQ=255)

                                                                                     gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACaaa       <  1:4448310/93‑1 (MQ=255)

                                                                                          aCCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGcccc  >  1:5270220/1‑93 (MQ=255)

CGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCC  >  NC_000913/2375128‑2375310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`