BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,920	G→A	N36N (AAC→AAT)	vacJ ←	lipoprotein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,920	0	G	A	87.9%	743.1 / 56.1	249	N36N (AAC→AAT)	vacJ	lipoprotein precursor
Reads supporting (aligned to +/- strand): ref base G (18/12); new base A (127/92); total (145/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

GCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGCAAGCGCCGA  >  NC_000913/2462828‑2463012

   gnTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTg                                                                                           <  1:3589324/93‑1 (MQ=255)

   ggTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTg                                                                                           >  2:3664514/1‑93 (MQ=255)

    gTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGa                                                                                          >  2:298548/1‑93 (MQ=255)

     ttGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGaa                                                                                         >  1:3069438/1‑93 (MQ=255)

     ttGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGaa                                                                                         >  1:4407560/1‑93 (MQ=255)

      tGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAAc                                                                                        <  1:5768089/93‑1 (MQ=255)

      tGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAAc                                                                                        <  1:4301731/93‑1 (MQ=255)

       gCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAAcc                                                                                       >  1:6233822/1‑93 (MQ=255)

         ggAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCt                                                                                     <  2:5560146/93‑1 (MQ=255)

         ggAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCt                                                                                     >  1:1164869/1‑93 (MQ=255)

         ggAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCt                                                                                     <  2:4629691/93‑1 (MQ=255)

           aaCATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTc                                                                                   >  2:4276528/1‑93 (MQ=255)

             cATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTa                                                                                 >  2:6200644/1‑93 (MQ=255)

              aTAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTaa                                                                                <  1:5354306/93‑1 (MQ=255)

              aTAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTaa                                                                                <  2:5028368/93‑1 (MQ=255)

               tAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAAc                                                                               <  2:6645221/93‑1 (MQ=255)

               tAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAAc                                                                               <  1:7510324/93‑1 (MQ=255)

               tAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAAc                                                                               <  1:1288022/93‑1 (MQ=255)

                aaTCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACg                                                                              >  1:1046961/1‑93 (MQ=255)

                aaTCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACg                                                                              <  2:7099258/93‑1 (MQ=255)

                 aTCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACgg                                                                             <  1:4941967/93‑1 (MQ=255)

                   cACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGt                                                                           <  2:7454556/93‑1 (MQ=255)

                    aCGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTc                                                                          >  2:2253615/1‑93 (MQ=255)

                      gCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAg                                                                        >  1:1996443/1‑93 (MQ=255)

                        cAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGag                                                                      >  2:7136298/1‑92 (MQ=255)

                          ggCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACg                                                                    >  1:2054569/1‑93 (MQ=255)

                             gACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGccc                                                                 >  1:386087/1‑93 (MQ=255)

                             gACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGccc                                                                 <  2:2726426/93‑1 (MQ=255)

                              aCAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCt                                                                >  2:4746763/1‑93 (MQ=255)

                              aCAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCt                                                                <  1:6218548/93‑1 (MQ=255)

                              aCAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCt                                                                <  2:2070868/93‑1 (MQ=255)

                              aCAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCt                                                                >  1:4483979/1‑93 (MQ=255)

                              aCAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCt                                                                >  1:4011222/1‑93 (MQ=255)

                               cAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCtt                                                               >  1:6527682/1‑93 (MQ=255)

                                 gCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGc                                                             >  1:3937202/1‑93 (MQ=255)

                                 gCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGc                                                             <  1:7151846/93‑1 (MQ=255)

                                   gACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTg                                                           >  2:6876816/1‑93 (MQ=255)

                                   gACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTg                                                           >  2:6023606/1‑93 (MQ=255)

                                    aCCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGa                                                          >  1:6675516/1‑93 (MQ=255)

                                    aCCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGa                                                          >  1:5168930/1‑93 (MQ=255)

                                    aCCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGa                                                          >  1:2860564/1‑93 (MQ=255)

                                      cGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATc                                                        >  2:5283423/1‑93 (MQ=255)

                                        gTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTg                                                      >  1:1725950/1‑93 (MQ=255)

                                        gTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTg                                                      <  1:4600904/93‑1 (MQ=255)

                                         tCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGt                                                     <  2:1105460/93‑1 (MQ=255)

                                           gAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc                                                   >  2:6993860/1‑93 (MQ=255)

                                            aaCAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAcc                                                  <  1:5972186/93‑1 (MQ=255)

                                            aaCAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAcc                                                  <  2:78131/93‑1 (MQ=255)

                                             aCAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCg                                                 >  2:4225589/1‑93 (MQ=255)

                                             aCAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCg                                                 <  1:7133131/93‑1 (MQ=255)

                                             aCAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCg                                                 <  1:3094649/93‑1 (MQ=255)

                                              cAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCgg                                                >  1:4116634/1‑93 (MQ=255)

                                               aaTATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGa                                               >  2:1580126/1‑93 (MQ=255)

                                               aaTATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGa                                               >  2:5359695/1‑93 (MQ=255)

                                               aaTATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGa                                               >  2:5918956/1‑93 (MQ=255)

                                                 tataCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAAc                                             >  1:7342558/1‑93 (MQ=255)

                                                 tataCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAAc                                             >  1:32684/1‑93 (MQ=255)

                                                 tataCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAAc                                             >  1:2901003/1‑93 (MQ=255)

                                                  ataCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACt                                            <  1:2385533/93‑1 (MQ=255)

                                                   taCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTc                                           <  2:4460187/93‑1 (MQ=255)

                                                   taCGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTc                                           >  2:6554276/1‑93 (MQ=255)

                                                       ggTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcac                                       >  1:2573899/1‑93 (MQ=255)

                                                        gTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcaca                                      <  1:4046569/93‑1 (MQ=255)

                                                        gTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcaca                                      >  2:2341492/1‑93 (MQ=255)

                                                         tCTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCacac                                     <  2:856399/93‑1 (MQ=255)

                                                          cTAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACAcc                                    >  2:5132174/1‑93 (MQ=255)

                                                           tAATACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACAccc                                   >  2:6625206/1‑93 (MQ=255)

                                                            aaTACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACAcccc                                  >  1:2661309/1‑93 (MQ=255)

                                                            aaTACATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACAcccc                                  >  2:1285180/1‑93 (MQ=255)

                                                              tACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCccac                                <  2:5493487/93‑1 (MQ=255)

                                                               aCATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCccacc                               <  1:5012727/93‑1 (MQ=255)

                                                                cATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCccacca                              >  2:3520319/1‑93 (MQ=255)

                                                                cATTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCccacca                              <  1:2778026/93‑1 (MQ=255)

                                                                 aTTGAAGTTGAAGTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAg                             >  2:1507531/1‑93 (MQ=255)

                                                                  ttgaagttgaagTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGa                            >  1:5430982/1‑93 (MQ=255)

                                                                  ttgaagttgaagTTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGa                            >  1:963748/1‑93 (MQ=255)

                                                                   tgaagttgaagtTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGaa                           <  1:1461746/93‑1 (MQ=255)

                                                                   tgaagttgaagtTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGaa                           >  1:6066791/1‑93 (MQ=255)

                                                                   tgaagttgaagtTGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGaa                           <  1:6851320/93‑1 (MQ=255)

                                                                    gaagttgaagttGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAg                          >  1:4748492/1‑93 (MQ=255)

                                                                    gaagttgaagttGTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAg                          >  2:3545964/1‑93 (MQ=255)

                                                                     aagttgaagttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGc                         <  2:695201/93‑1 (MQ=255)

                                                                      agttgaagttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCg                        >  2:2440679/1‑93 (MQ=255)

                                                                      agttgaagttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCg                        <  2:4566012/93‑1 (MQ=255)

                                                                          gaagttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGt                    >  1:3528506/1‑93 (MQ=255)

                                                                             gttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTcc                 >  2:3215453/1‑93 (MQ=255)

                                                                             gttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTcc                 <  1:1577973/93‑1 (MQ=255)

                                                                              ttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTccc                >  1:4840463/1‑93 (MQ=255)

                                                                              ttgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTccc                >  2:5227149/1‑93 (MQ=255)

                                                                               tgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTCCCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCa               <  1:3747165/93‑1 (MQ=255)

                                                                               tgTACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCa               <  2:2492973/93‑1 (MQ=255)

                                                                                 tACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCaga             >  2:2787682/1‑93 (MQ=255)

                                                                                 tACATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCaga             >  1:5494344/1‑93 (MQ=255)

                                                                                   cATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGc           <  2:3451168/93‑1 (MQ=255)

                                                                                   cATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGc           >  1:1640801/1‑93 (MQ=255)

                                                                                   cATGGTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGc           >  1:3375548/1‑93 (MQ=255)

                                                                                      ggTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGCAAg        <  1:1340639/93‑1 (MQ=255)

                                                                                       gTGCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGCAAgc       <  1:4775896/93‑1 (MQ=255)

                                                                                         gCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCATAAGCGTAATTCCCAGAGCAAgcgc     <  2:7362557/93‑1 (MQ=255)

                                                                                         gCGATTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGCAAgcgc     <  1:5269311/93‑1 (MQ=255)

GCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACCCCACCAGAAGCGTAGTTCCCAGAGCAAGCGCCGA  >  NC_000913/2462828‑2463012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`