BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,463	T→C	V258A (GTG→GCG)	b2380 →	putative sensor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,463	0	T	C	85.7%	719.9 / 109.6	307	V258A (GTG→GCG)	b2380	putative sensor protein
Reads supporting (aligned to +/- strand): ref base T (23/21); new base C (115/148); total (138/169)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 311 total aligned reads displayed.

ATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACA  >  NC_000913/2497371‑2497552

aTTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGt                                                                                           <  2:7023676/93‑1 (MQ=255)

aTTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGt                                                                                           >  1:2807733/1‑93 (MQ=255)

    cGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGt                                                                                       <  2:6963538/93‑1 (MQ=255)

     gCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTg                                                                                      >  2:2498959/1‑93 (MQ=255)

     gCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTg                                                                                      <  2:2682406/93‑1 (MQ=255)

      cAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGa                                                                                     >  1:765644/1‑93 (MQ=255)

        aGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAAc                                                                                   >  2:3922286/1‑93 (MQ=255)

         ggTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACa                                                                                  >  2:4007065/1‑93 (MQ=255)

           tCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATa                                                                                >  2:3441199/1‑93 (MQ=255)

             tGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAAc                                                                              >  1:7329725/1‑93 (MQ=255)

             tGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAAc                                                                              >  2:3244609/1‑93 (MQ=255)

               cGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTa                                                                            <  1:5428259/93‑1 (MQ=255)

               cGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTa                                                                            >  1:6605994/1‑93 (MQ=255)

                gAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTAt                                                                           >  2:6002640/1‑93 (MQ=255)

                gAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTAt                                                                           >  1:5861800/1‑93 (MQ=255)

                gAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTAt                                                                           <  2:7270413/93‑1 (MQ=255)

                  aaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCa                                                                         <  1:4776043/93‑1 (MQ=255)

                  aaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCa                                                                         <  1:7181919/93‑1 (MQ=255)

                  aaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCa                                                                         >  1:2263637/1‑93 (MQ=255)

                  aaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCa                                                                         <  2:1390808/93‑1 (MQ=255)

                  aaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCa                                                                         <  1:6022433/93‑1 (MQ=255)

                   aTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAg                                                                        >  1:3130241/1‑93 (MQ=255)

                     tATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGaa                                                                      <  1:413424/93‑1 (MQ=255)

                       tCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATg                                                                    <  1:4823821/93‑1 (MQ=255)

                           cgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGa                                                                <  1:1622390/93‑1 (MQ=255)

                            ggtGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGAt                                                               >  2:3570547/3‑93 (MQ=255)

                            gATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGAt                                                               >  2:1816990/1‑93 (MQ=255)

                            gATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGAt                                                               <  1:1549759/93‑1 (MQ=255)

                              tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGa                                                             >  2:2860851/1‑93 (MQ=255)

                              tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGa                                                             <  2:2577035/93‑1 (MQ=255)

                              tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGa                                                             <  2:4501794/93‑1 (MQ=255)

                               gACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGAc                                                            <  2:4243829/93‑1 (MQ=255)

                                aCATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACt                                                           >  2:7531245/1‑93 (MQ=255)

                                 cATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACtt                                                          >  1:2389206/1‑93 (MQ=255)

                                 cATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACtt                                                          >  1:553132/1‑93 (MQ=255)

                                  aTTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTc                                                         <  2:1314191/93‑1 (MQ=255)

                                  aTTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTc                                                         <  2:3026680/93‑1 (MQ=255)

                                  aTTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTc                                                         >  1:7029940/1‑93 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        >  2:6281222/1‑93 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        <  2:5593468/93‑1 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        >  1:3808919/1‑93 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        >  2:913420/1‑93 (MQ=255)

                                    tCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAt                                                       <  1:4637520/93‑1 (MQ=255)

                                    tCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAt                                                       >  1:6693135/1‑93 (MQ=255)

                                    tCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAt                                                       <  2:6659312/93‑1 (MQ=255)

                                     cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAtt                                                      <  2:3658749/93‑1 (MQ=255)

                                      aCGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTa                                                     <  1:5292946/93‑1 (MQ=255)

                                      aCGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTa                                                     <  1:2881419/93‑1 (MQ=255)

                                      aCGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTa                                                     >  1:1998128/1‑93 (MQ=255)

                                      aCGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTa                                                     >  2:4914272/1‑93 (MQ=255)

                                        gCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGc                                                   <  1:6654581/93‑1 (MQ=255)

                                         ccGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGcc                                                  >  2:6860890/1‑93 (MQ=255)

                                          cGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGccc                                                 <  2:5911088/93‑1 (MQ=255)

                                           gATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCg                                                >  1:1887131/1‑93 (MQ=255)

                                             tGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGAc                                              <  1:1383438/93‑1 (MQ=255)

                                             tGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGAc                                              <  2:1938597/93‑1 (MQ=255)

                                                 gTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAcc                                          <  1:6241191/93‑1 (MQ=255)

                                                      gATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCa                                     >  2:7431628/1‑93 (MQ=255)

                                                      gATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCa                                     <  2:5223835/93‑1 (MQ=255)

                                                      gATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCa                                     <  1:2451687/93‑1 (MQ=255)

                                                       aTTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAg                                    <  1:5930903/93‑1 (MQ=255)

                                                       aTTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAg                                    <  1:7251840/93‑1 (MQ=255)

                                                         tACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGc                                  <  1:6295549/93‑1 (MQ=255)

                                                         tACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGc                                  <  2:5119745/93‑1 (MQ=255)

                                                         tACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGc                                  <  2:6399639/93‑1 (MQ=255)

                                                           cTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGa                                >  2:5933438/1‑93 (MQ=255)

                                                           cTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGa                                <  2:5306582/93‑1 (MQ=255)

                                                            tAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGAt                               >  1:6477806/1‑93 (MQ=255)

                                                             aaTACCGATGATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATg                              <  2:1148905/93‑1 (MQ=255)

                                                             aaTACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATg                              <  1:4404989/93‑1 (MQ=255)

                                                              aTACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGa                             <  1:3064652/93‑1 (MQ=255)

                                                              aTACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGa                             <  1:1682929/93‑1 (MQ=255)

                                                               tACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTTTCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa                            <  1:3446826/93‑1 (MQ=255)

                                                               tACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa                            <  1:1003426/93‑1 (MQ=255)

                                                                aCCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt                           <  1:2111918/93‑1 (MQ=255)

                                                                aCCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt                           <  2:5731588/93‑1 (MQ=255)

                                                                 ccGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAtt                          <  1:4528414/93‑1 (MQ=255)

                                                                 ccGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAtt                          >  1:6984319/1‑93 (MQ=255)

                                                                  cGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTa                         >  1:6817260/1‑93 (MQ=255)

                                                                   gATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTAc                        >  2:5020711/1‑93 (MQ=255)

                                                                   gATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTAc                        >  1:4029963/1‑93 (MQ=255)

                                                                   gATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTAc                        >  2:6142537/1‑93 (MQ=255)

                                                                    aTCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACg                       <  1:1329568/93‑1 (MQ=255)

                                                                     tCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACgg                      >  2:2614672/1‑93 (MQ=255)

                                                                      cATGTGCTGGCCTATGTTGGCACGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGa                     <  1:1517652/93‑1 (MQ=255)

                                                                          tgCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaaa                 <  1:5117770/93‑1 (MQ=255)

                                                                          tgCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaaa                 <  2:2378639/93‑1 (MQ=255)

                                                                             tGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatca              >  2:1002340/1‑93 (MQ=255)

                                                                              ggCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatcat             >  2:7196392/1‑93 (MQ=255)

                                                                               gCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatcatc            >  2:1027368/1‑93 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTCCCCGTCAGGCGATGAATTACGGAAAAAtcatca           <  2:4712990/93‑1 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAAtcatca           <  2:4992760/93‑1 (MQ=255)

                                                                                 cTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATcatcat          <  2:4610283/93‑1 (MQ=255)

                                                                                   aTGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTa        >  1:49568/1‑93 (MQ=255)

                                                                                   aTGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTa        >  2:378173/1‑93 (MQ=255)

                                                                                   aTGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTa        <  1:5217657/93‑1 (MQ=255)

                                                                                    tGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTaa       >  2:5440320/1‑93 (MQ=255)

                                                                                      ttGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTaaaa     >  2:2280155/1‑93 (MQ=255)

                                                                                        ggCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAAc   <  1:5618816/93‑1 (MQ=255)

                                                                                         gcgcGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTGCCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACa  <  1:3521387/93‑1 (MQ=255)

ATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACA  >  NC_000913/2497371‑2497552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`