BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,955	T→C	D143D (GAT→GAC)	yafA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,955	0	T	C	81.8%	517.8 / 116.3	252	D143D (GAT→GAC)	yafA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (26/20); new base C (118/88); total (144/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

Only 100 of 255 total aligned reads displayed.

GTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTT  >  NC_000913/256864‑257045

gtgCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATg                                                                                           >  1:5817786/1‑93 (MQ=255)

  gCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAgacgac                                                                                         <  2:6342383/93‑1 (MQ=255)

  gCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAgacgac                                                                                         <  2:4334323/93‑1 (MQ=255)

   cGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGAcc                                                                                        <  1:81567/93‑1 (MQ=255)

    ggAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCt                                                                                       >  2:4573542/1‑93 (MQ=255)

    ggAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCt                                                                                       <  2:2970363/93‑1 (MQ=255)

    ggAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCt                                                                                       <  2:1065231/93‑1 (MQ=255)

     gAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTg                                                                                      >  1:3881474/1‑93 (MQ=255)

     gAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTg                                                                                      >  1:1789030/1‑93 (MQ=255)

      aaGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTgg                                                                                     >  1:6362000/1‑93 (MQ=255)

       aGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGc                                                                                    <  2:6051048/93‑1 (MQ=255)

        gACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGcc                                                                                   <  2:6971957/93‑1 (MQ=255)

        gACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGcc                                                                                   >  2:1224357/1‑93 (MQ=255)

          cGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGTCCGa                                                                                 <  2:6571494/93‑1 (MQ=255)

            aTCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACATTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGc                                                                               >  2:191794/1‑93 (MQ=255)

               cGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAg                                                                            <  2:2708287/93‑1 (MQ=255)

                gCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAgc                                                                           >  2:1983504/1‑93 (MQ=255)

                  aaCTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAgcgc                                                                         >  2:5313451/1‑93 (MQ=255)

                  aaCTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAgcgc                                                                         >  2:5651634/1‑93 (MQ=255)

                   aCTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCa                                                                        <  2:4504581/93‑1 (MQ=255)

                   aCTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCa                                                                        >  1:3783503/1‑93 (MQ=255)

                    cTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAg                                                                       <  1:6881672/93‑1 (MQ=255)

                     tCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAgg                                                                      >  2:5712725/1‑93 (MQ=255)

                     tCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAgg                                                                      >  1:1762306/1‑93 (MQ=255)

                     tCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAgg                                                                      >  1:6814760/1‑93 (MQ=255)

                     tCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAgg                                                                      >  1:6994400/1‑93 (MQ=255)

                     tCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAgg                                                                      >  1:6499277/1‑93 (MQ=255)

                        gTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCtt                                                                   <  2:51688/93‑1 (MQ=255)

                        gTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCtt                                                                   >  2:6838028/1‑93 (MQ=255)

                         tGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCttt                                                                  <  1:3105259/93‑1 (MQ=255)

                         tGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCttt                                                                  >  1:5304996/1‑93 (MQ=255)

                                cACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAAc                                                           <  2:4703113/93‑1 (MQ=255)

                                cACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAAc                                                           <  2:3284420/93‑1 (MQ=255)

                                 aCTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAAcc                                                          >  1:3360488/1‑93 (MQ=255)

                                 aCTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAAcc                                                          <  2:3749626/93‑1 (MQ=255)

                                  cTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                                                         >  1:3489450/1‑93 (MQ=255)

                                  cTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                                                         >  1:270020/1‑93 (MQ=255)

                                   tGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc                                                        >  1:5591372/1‑93 (MQ=255)

                                   tGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc                                                        <  2:2934985/93‑1 (MQ=255)

                                   tGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc                                                        <  1:1445459/93‑1 (MQ=255)

                                   tGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcgc                                                        <  2:6492178/93‑1 (MQ=255)

                                     gCTGCATGCGGCTACGTTGTACAACATTGCTGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCgcgc                                                      <  2:6366416/93‑1 (MQ=255)

                                     gCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCgcgc                                                      >  2:1664805/1‑93 (MQ=255)

                                      cTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGcc                                                     <  2:2103230/93‑1 (MQ=255)

                                      cTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGcc                                                     <  2:3957038/93‑1 (MQ=255)

                                       tGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCt                                                    >  1:655335/1‑93 (MQ=255)

                                        gCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAGGCGCAGGCTTTGTCAAACCGCGCCTa                                                   <  2:823780/93‑1 (MQ=255)

                                         cATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTAt                                                  <  1:5479179/93‑1 (MQ=255)

                                         cATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTAt                                                  <  1:898704/93‑1 (MQ=255)

                                          aTGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATg                                                 >  1:2135703/1‑93 (MQ=255)

                                          aTGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATg                                                 <  2:1376863/93‑1 (MQ=255)

                                           tGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGa                                                >  1:6867964/1‑93 (MQ=255)

                                            gCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTGTGaa                                               <  2:3713342/93‑1 (MQ=255)

                                            gCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGaa                                               >  2:3997157/1‑93 (MQ=255)

                                               gCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAagag                                            <  1:1057471/93‑1 (MQ=255)

                                                cTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAgg                                           <  1:2258327/93‑1 (MQ=255)

                                                cTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAgg                                           >  2:5786250/1‑93 (MQ=255)

                                                 tACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                                          >  2:28768/1‑93 (MQ=255)

                                                 tACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                                          <  1:7368914/93‑1 (MQ=255)

                                                 tACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                                          >  2:149281/1‑93 (MQ=255)

                                                 tACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                                          >  1:4634447/1‑93 (MQ=255)

                                                 tACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGc                                          <  1:4877907/93‑1 (MQ=255)

                                                  aCGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGcc                                         <  2:5235448/93‑1 (MQ=255)

                                                    gTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGc                                       <  1:4084469/93‑1 (MQ=255)

                                                    gTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGc                                       >  1:2299399/1‑93 (MQ=255)

                                                      tGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTc                                     >  2:7279260/1‑93 (MQ=255)

                                                       gTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCa                                    >  1:3198863/1‑93 (MQ=255)

                                                       gTACAACATTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCa                                    >  1:5327947/1‑93 (MQ=255)

                                                         acaacaTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGc                                  <  2:132851/93‑1 (MQ=255)

                                                         acaacaTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGc                                  >  1:5728406/1‑93 (MQ=255)

                                                         acaacaTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGc                                  >  2:4969053/1‑93 (MQ=255)

                                                          caacaTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCg                                 >  1:2122163/1‑93 (MQ=255)

                                                            acaTTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTc                               <  1:5962378/93‑1 (MQ=255)

                                                            acaTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTc                               <  1:1549840/93‑1 (MQ=255)

                                                             caTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCt                              >  1:4567075/1‑93 (MQ=255)

                                                             caTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCt                              >  2:5433194/1‑93 (MQ=255)

                                                              aTTGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTa                             <  1:5009598/93‑1 (MQ=255)

                                                               ttGCCGCCTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTAc                            <  2:2280331/93‑1 (MQ=255)

                                                                  cctccTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCCCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCgg                         <  1:5018498/90‑1 (MQ=255)

                                                                  ccgccTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCgg                         <  2:707843/93‑1 (MQ=255)

                                                                     ccTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCa                      >  2:4264969/1‑93 (MQ=255)

                                                                     ccTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCa                      <  1:3853294/93‑1 (MQ=255)

                                                                     ccTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCa                      >  1:5976253/1‑93 (MQ=255)

                                                                     ccTATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCa                      >  2:2355441/1‑93 (MQ=255)

                                                                      cTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCAc                     >  1:6663399/1‑93 (MQ=255)

                                                                       tATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACg                    >  2:3409982/1‑93 (MQ=255)

                                                                       tATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACg                    >  1:6581251/1‑93 (MQ=255)

                                                                       tATCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACg                    >  1:6294513/1‑93 (MQ=255)

                                                                        aTCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGa                   >  1:1236681/1‑93 (MQ=255)

                                                                         tCCTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGAt                  >  1:317531/1‑93 (MQ=255)

                                                                           cTCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGc                <  1:5056488/93‑1 (MQ=255)

                                                                            tCATCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCg               >  1:2799418/1‑93 (MQ=255)

                                                                               tCTGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCa            >  2:5894558/1‑93 (MQ=255)

                                                                                 tGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGa          >  1:2888453/1‑93 (MQ=255)

                                                                                 tGAAAGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGa          >  1:4762923/1‑93 (MQ=255)

                                                                                    aaGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTAGGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATgg       >  1:7299608/1‑93 (MQ=255)

                                                                                    aaGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATgg       <  1:4359413/93‑1 (MQ=255)

                                                                                     aGGAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGa      >  2:4490220/1‑93 (MQ=255)

                                                                                      ggAGACGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAg     >  1:322197/1‑93 (MQ=255)

                                                                                         gaTGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGttt  >  2:2512586/1‑93 (MQ=255)

GTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTACCGGGCACGATGCGGCAGATGGAGTTT  >  NC_000913/256864‑257045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`